Characterization of surface sugars on algal cells with fluorescein isothiocyanate-conjugated lectins.

abstract：:Lemna species are reported to accumulate a variety of metals from contaminated/polluted sites. Cadmium is a nonessential element for plant metabolism. In this work, we aimed to investigate physiological responses to low doses of cadmium (up to 100 microM). From exposure to the lowest Cd concentration (1 microM) to the...

journal_title：Protoplasma

authors： Malec P,Maleva MG,Prasad MN,Strzałka K

更新日期：2010-04-01 00:00:00

abstract：:In the present investigation, we have used adenosine triphosphatase (ATPase) activity as biochemical test of toxic action of lindane that was explained by lipid peroxidation model. Study was also undertaken to ascertain the potential protective role of alpha-lipoic acid (ALA) and vitamin E on the same parameters. High...

journal_title：Protoplasma

authors： Bist R,Misra S,Bhatt DK

更新日期：2010-06-01 00:00:00

abstract：:Heat shock protein 90 (Hsp90) is an abundant cytosolic protein. In higher eukaryotes two isoforms of Hsp90 exist, Hsp90 alpha and Hsp90 beta. Hsp90 was purified from rat liver and after sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed a double band at about 90 kDa. The two bands were separated and id...

journal_title：Protoplasma

authors： Langer T,Fasold H

更新日期：2001-01-01 00:00:00

abstract：:Narcissus tazetta is one of the major geophyte crops worldwide, but little is known about its cell biology. The narcissus storage organ was studied by monitoring scale cell biology during the growth stage and dormancy, and it was found that amyloplasts gradually increased in size and reached a maximum at dormancy. In ...

journal_title：Protoplasma

authors： Zaffryar S,Zimerman B,Abu-Abied M,Belausov E,Lurya G,Vainstein A,Kamenetsky R,Sadot E

更新日期：2007-01-01 00:00:00

abstract：:In the Cape Fynbos of South Africa, Psoralea pinnata (L.) plants occur naturally in both wetland and well-drained soils and yet effectively fix N2 under the two contrasting conditions. In this study, nodule structure and functioning in P. pinnata plants from the two habitats were evaluated using light and transmission...

journal_title：Protoplasma

authors： Kanu SA,Dakora FD

更新日期：2017-01-01 00:00:00

abstract：:The osmotic potential and effects of plasmolysis were investigated in two different Klebsormidium strains from alpine habitats by incubation in 300-2,000 (3,000) mM sorbitol. Several members of this genus were previously found to tolerate desiccation in the vegetative state yet information was lacking on the osmotic p...

journal_title：Protoplasma

authors： Kaplan F,Lewis LA,Wastian J,Holzinger A

更新日期：2012-07-01 00:00:00

abstract：:Kinesin superfamily proteins are important microtubule-based motor proteins with a kinesin motor domain that is conserved among all eukaryotic organisms. They are responsible for unidirectionally transporting various cargoes, including membranous organelles, protein complexes, and mRNAs. They also play critical roles ...

journal_title：Protoplasma

authors： Li J,Xu Y,Chong K

更新日期：2012-06-01 00:00:00

abstract：:Present communication reports laboratory and pot experiments conducted to study the influence of water and osmotic stress on nitrogen uptake and metabolism in two wheat (Triticum aestivum L) cultivars with and without potassium supplementation. Polyethylene glycol 6000-induced osmotic stress/restricted irrigation caus...

journal_title：Protoplasma

authors： Ahanger MA,Tittal M,Mir RA,Agarwal RM

更新日期：2017-09-01 00:00:00

abstract：:In order to corroborate the hypothesis that variations in the rate of spontaneous ultra-weak photon emission (UPE) from germinating seedlings are related to local variations of the lunisolar tidal force, a series of simultaneous tests was performed using the time courses of UPE collected from three plant species-corn,...

journal_title：Protoplasma

authors： Gallep CM,Barlow PW,Burgos RC,van Wijk EP

更新日期：2017-01-01 00:00:00

abstract：:Tradescantia virginiana leaf epidermal cells were plasmolysed by sequential treatment with 0.8 M and 0.3 M sucrose. Plasmolysis revealed adhesion of the plasma membrane to the cell wall at sites coinciding with cytoskeletal arrays involved in the polarisation of cells undergoing asymmetric divisions--cortical actin pa...

journal_title：Protoplasma

authors： Cleary AL

更新日期：2001-01-01 00:00:00

abstract：:The present study focuses on the responses of floating laminae of the Mn-tolerant hydrophyte Trapa natans L. to 1 mM Mn and their ability to accumulate the metal. Studies were carried out first on young floating laminae belonging to the second verticil of 30-day-old plants which originated from fruits that had been ma...

journal_title：Protoplasma

authors： Baldisserotto C,Ferroni L,Anfuso E,Pagnoni A,Fasulo MP,Pancaldi S

更新日期：2007-01-01 00:00:00

abstract：:Mechanisms of transport of secretory products across the Golgi apparatus (GA) as well as of scale formation in prymnesiophytes have remained controversial. We have used a quantitative morphological approach to study formation and transport of scales across the GA in haploid cells of Pleurochrysis sp. The GA of these c...

journal_title：Protoplasma

authors： Hawkins EK,Lee JJ

更新日期：2001-01-01 00:00:00

abstract：:The presence of specific protein types in claws and beaks of turtles is poorly known. The present immunological study describes the localization of some of the main corneous beta proteins (CBPs) coded in the epidermal differentiation complex of turtles. Three antibodies here utilized revealed that glycine-, cysteine-,...

journal_title：Protoplasma

authors： Alibardi L

更新日期：2020-07-01 00:00:00

abstract：:Features of programmed cell death (PCD) and dynamic changes of starch accumulation in developing pericarp cells of wheat (Triticum aestivum L.) were observed and analyzed by periodic acid-Schiff/toluidine blue O double staining, fluorescence staining, terminal deoxynucleotidyl transferase-mediated fluorescein deoxyuri...

journal_title：Protoplasma

authors： Zhou Z,Wang L,Li J,Song X,Yang C

更新日期：2009-07-01 00:00:00

abstract：:Sugarcane shows reduced crop stand under relatively suboptimal conditions; the main reason for this is its sensitivity to ionic stress in the soil solution. This research was performed to explore some physiological and developmental changes in the immature sugarcane buds submitted to salt stress and possible role of g...

journal_title：Protoplasma

authors： Rasheed R,Wahid A,Hussain I,Mahmood S,Parveen A

更新日期：2016-05-01 00:00:00

abstract：:The couplons of the cardiomyocyte form nanospaces within the cell that place the L-type calcium channel (Ca(v)1.2), situated on the plasmalemma, in opposition to the type 2 ryanodine receptor (RyR2), situated on the sarcoplasmic reticulum. These two molecules, which form the basis of excitation-contraction coupling, a...

journal_title：Protoplasma

authors： Asghari P,Scriven DR,Hoskins J,Fameli N,van Breemen C,Moore ED

更新日期：2012-02-01 00:00:00

abstract：:The dinoflagellate Crypthecodinium cohnii Biecheler propagates by both binary and multiple fission. By a newly developed mutagenesis protocol based on using ethyl methanesulfonate and a cell size screening method, a cell cycle mutant, mf2, was isolated with giant cells which predominantly divide by multiple fission. T...

journal_title：Protoplasma

authors： Lam CM,Chong C,Wong JT

更新日期：2001-01-01 00:00:00

abstract：:Through reverse transcription-polymerase chain reaction and Northern blot analysis, we identified TBK5, a novel plant-specific kinesin-like protein (KLP) that is highly expressed in interphase tobacco BY-2 cells. TBK5 mRNA was present at a high level throughout the growth cycle, even in cells that had entered the stat...

journal_title：Protoplasma

authors： Matsui K,Collings D,Asada T

更新日期：2001-01-01 00:00:00

abstract：:Unconventional protein secretion (UPS) is a collective term for mechanisms by which cytosolic proteins that lack a signal peptide ("leaderless secretory proteins" (LSPs)) can gain access to the cell exterior. Numerous examples of UPS have been well documented in animal and yeast cells. In contrast, our understanding o...

journal_title：Protoplasma

authors： Robinson DG,Ding Y,Jiang L

更新日期：2016-01-01 00:00:00

abstract：:Earwigs (Dermaptera) use different strategies to increase their reproductive success. Most species lay eggs; however, viviparity of the matrotrophic type has been reported in two groups: Hemimeridae and Arixeniidae. In Arixeniidae, offspring develop in two separate places: inside an ovary (the intraovarian phase) and ...

journal_title：Protoplasma

authors： Jaglarz MK,Tworzydlo W,Rak A,Kotula-Balak M,Sekula M,Bilinski SM

更新日期：2019-11-01 00:00:00

abstract：:Salinity is a major stress that adversely affects plant growth and crop production. Understanding the cellular responses and molecular mechanisms by which plants perceive and adopt salinity stress is of fundamental importance. In this work, some of the cellular signaling events including cell death, reactive oxygen sp...

journal_title：Protoplasma

authors： Li Z,Yang H,Wu X,Guo K,Li J

更新日期：2015-05-01 00:00:00

abstract：:With the goal of evaluating the factors that may be influencing the reproductive success of an endangered, terrestrial orchid Cypripedium japonicum Thunb., the anatomical and morphological characteristics of seed development were assessed, as were the levels of endogenous hormones to investigate the possible relations...

journal_title：Protoplasma

authors： Yan X,Tian M,Liu F,Wang C,Zhang Y

更新日期：2017-11-01 00:00:00

abstract：:Octanal is a potential alternative to chemical fungicides in controlling postharvest disease of citrus fruit. In this study, the antifungal activity and the underlying mechanism of octanal against spore germination of Penicillium digitatum, one of the main postharvest pathogens in citrus, were investigated. Results sh...

journal_title：Protoplasma

authors： Dou S,Liu S,Xu X,OuYang Q,Tao N

更新日期：2017-07-01 00:00:00

abstract：:The effect of cytochalasin B (CB; 25 micrograms ml-1 in 1% dimethylsulfoxide, DMSO) upon the structural polarity of statocytes in cress roots is demonstrated. If normal, vertically grown roots are incubated in CB, the structural polarity of the statocytes is altered according to the developmental stage of the root. St...

journal_title：Protoplasma

authors： Hensel W

更新日期：1985-01-01 00:00:00

abstract：:Microtubule dynamics were examined in live cells of the fungal plant pathogen Magnaporthe grisea transformed for constitutive expression of a fusion protein containing enhanced yellow-fluorescent protein and a Neurospora crassa benomyl-resistant allele of beta-tubulin. Transformants retained their ability to different...

journal_title：Protoplasma

authors： Czymmek KJ,Bourett TM,Shao Y,DeZwaan TM,Sweigard JA,Howard RJ

更新日期：2005-04-01 00:00:00

abstract：:Phenolics are involved in many of plants' biological functions. In particular, they play important roles in determining the quality of grape berries and the wine made from them, and can also act as antioxidants with beneficial effects for human health. Several enzymes are involved in the synthesis of phenolic compound...

journal_title：Protoplasma

authors： Molero de Ávila ME,Alarcón MV,Uriarte D,Mancha LA,Moreno D,Francisco-Morcillo J

更新日期：2019-01-01 00:00:00

abstract：:Indirect immunofluorescence and confocal microscopy were used to study the nucleation and organization of microtubules during meiosis in two species of leafy liverworts, Cephalozia macrostachya and Telaranea longifolia. This is the first such study of sporogenesis in the largest group of liverworts important as living...

journal_title：Protoplasma

authors： Brown RC,Lemmon BE

更新日期：2009-10-01 00:00:00

abstract：:Previous complementation of a chitin synthase class Vb null mutant (Colletotrichum graminicola chsA) indicated that the encoded protein is responsible for approximately 30% of the conidial chitin, is essential for conidial wall strength in media with high water potential, and contributes to strength of hyphal tips. We...

journal_title：Protoplasma

authors： Amnuaykanjanasin A,Epstein L

更新日期：2006-05-01 00:00:00

abstract：:Fruit shape and ripening are major horticultural traits for many fruits and vegetable crops. Changes in fruit shape and ripening are often accomplished by altered cell division or cell expansion patterns. Gibberellic acids (GAs) are essential for tomato fruit development; however, the exact role and the underlying mec...

journal_title：Protoplasma

authors： Chen S,Wang XJ,Tan GF,Zhou WQ,Wang GL

更新日期：2020-05-01 00:00:00

abstract：:We have shown previously that the durum wheat TdSOS1 excludes Na+ and Li+ ions outside cells. Moreover, this protein is activated by Arabidopsis kinase SOS2 through phosphorylation. The elimination of both SOS2 phosphorylation sites and the auto-inhibitory domain produces a hyperactive TdSOS1∆972 form, which have a ma...

journal_title：Protoplasma

authors： Feki K,Tounsi S,Masmoudi K,Brini F

更新日期：2017-07-01 00:00:00