Abstract：
:We have shown previously that the durum wheat TdSOS1 excludes Na+ and Li+ ions outside cells. Moreover, this protein is activated by Arabidopsis kinase SOS2 through phosphorylation. The elimination of both SOS2 phosphorylation sites and the auto-inhibitory domain produces a hyperactive TdSOS1∆972 form, which have a maximal activity independent from the regulatory SOS2/SOS3 complex. We demonstrated that the expression of TdSOS1 enhances salt tolerance of the transgenic Arabidopsis plants. In this study, we analyzed the response to H2O2-induced oxidative stress of the transgenic Arabidopsis expressing one of the two TdSOS1 forms. Firstly, we showed that the exogenous H2O2 treatment leads to an accumulation of SOS1 transcripts in leaves and roots of the durum wheat and also in the transgenic plants. These transgenic plants showed significant oxidative stress tolerance compared to control plants, especially the plants expressing the hyperactive form. This tolerance was manifested by high proline accumulation and low malonyldialdehyde (MDA), O2˙- and H2O2 contents. Furthermore, the activities of three essential ROS scavenging enzymes (SOD, CAT, and POD) were higher in the transgenic plants under oxidative stress, as compared to control plants. Taken together, these data suggested that TdSOS1 plays a crucial role in response to oxidative stress.
journal_name
Protoplasmajournal_title
Protoplasmaauthors
Feki K,Tounsi S,Masmoudi K,Brini Fdoi
10.1007/s00709-016-1066-8subject
Has Abstractpub_date
2017-07-01 00:00:00pages
1725-1734issue
4eissn
0033-183Xissn
1615-6102pii
10.1007/s00709-016-1066-8journal_volume
254pub_type
杂志文章相关文献
PROTOPLASMA文献大全abstract：:Flagellar development in the plurilocular zoidangia of sporophytes of the brown alga Ectocarpus siliculosus was analyzed in detail using transmission electron microscopy and electron tomography. A series of cell divisions in the plurilocular zoidangia produced the spore-mother cells. In these cells, the centrioles dif...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-012-0405-7
更新日期：2013-02-01 00:00:00
abstract：:In this study, we have shown the applicability of chloramphenicol acetyltransferase as a new and convenient selectable marker for stable nuclear transformation as well as potential chloroplast transformation of Cyanidioschyzon merolae-a new model organism, which offers unique opportunities for studding the mitochondri...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-015-0936-9
更新日期：2017-01-01 00:00:00
abstract：:Investigation of 67 gymnosperm and angiosperm species belonging to 25 orders shows that epistomatal mucilage plugs are a widespread phenomenon. Measurements of the leaf water status by using the leaf patch clamp pressure technique suggest that the mucilage plugs are involved in moisture uptake and buffering leaf cells...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-008-0029-0
更新日期：2009-03-01 00:00:00
abstract：:Salt-stress-induced alterations in osmotic, ionic, and redox responses were studied in the early period of treatment (30 min to 5 days) in seedlings of Brassica juncea L. Roots and shoots under mild (50 mM) and severe (250 mM) NaCl stress were analyzed for growth, oxidative stress, osmolyte accumulation, antioxidant d...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-015-0792-7
更新日期：2016-01-01 00:00:00
abstract：:This article deals with the distribution of callose and of the homogalacturonan (HG) epitopes recognized by LM20, JIM5, and 2F4 antibodies in cell walls of differentiating and functioning stomatal complexes of the monocotyledon Zea mays and the dicotyledon Vigna sinensis. The findings revealed that, during stomatal de...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-019-01425-8
更新日期：2020-01-01 00:00:00
abstract：:Yr36 is an important gene conferring resistance to stripe rust of wheat caused by Puccinia striiformis f. sp. tritici (Pst). To determine if the Yr36 resistance is correlated to reactive oxygen species (ROS) burst and cell death, wheat near-isogenic lines with (UC1041 + Yr36) and without (UC1041) the gene were histolo...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-015-0833-2
更新日期：2016-05-01 00:00:00
abstract：:The classification of order Nostocales (Cyanobacteria) and inter relationships of morphologically similar taxa is still debatable due to ever changing morphological features. No attempt has been made to improve the morphological taxonomy despite the fact that it is the morphology that represents the totality of genes....
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-014-0629-9
更新日期：2015-01-01 00:00:00
abstract：:Endoplasmic reticulum (ER) is a major site for secretory protein folding and lipid synthesis. Since ER cannot be synthesized de novo, it must be inherited during the cell cycle. Studying ER inheritance can however be difficult because the ER of typical plant and animal cells is morphologically complex. Therefore, our ...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-011-0359-1
更新日期：2012-10-01 00:00:00
abstract：:We used an ultraviolet microbeam to cut individual kinetochore spindle fibres in metaphase crane-fly spermatocytes. We then followed the growth of the "kinetochore stubs", the remnants of kinetochore fibres that remain attached to kinetochores. Kinetochore stubs elongate with constant velocity by adding tubulin subuni...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-007-0265-8
更新日期：2007-01-01 00:00:00
abstract：:We have analyzed the expression of key genes orchestrating tail regeneration in lizard under normal and scarring conditions after cauterization. At 1-day post-cauterization (1 dpc), the injured blastema contains degenerating epithelial and mesenchymal cells, numerous mast cells, and immune cells. At 3 and 7 dpc, a str...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-020-01545-6
更新日期：2021-01-01 00:00:00
abstract：:Phenolics are involved in many of plants' biological functions. In particular, they play important roles in determining the quality of grape berries and the wine made from them, and can also act as antioxidants with beneficial effects for human health. Several enzymes are involved in the synthesis of phenolic compound...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-018-1278-1
更新日期：2019-01-01 00:00:00
abstract：:Hepatocellular carcinoma (HCC) is the most common primary malignancy of the liver and is characterized by multistage formation. The presence of ductular reaction, cytokeratin 7 positivity (PCK7), and increased levels of gamma glutamyltransferase (γGT) has been observed during liver carcinogenesis and contribute to tum...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-016-1000-0
更新日期：2017-03-01 00:00:00
abstract：:Cell polarity, the asymmetric organization of cellular components along one or multiple axes, is present in most cells. From budding yeast cell polarization induced by pheromone signaling, oocyte polarization at fertilization to polarized epithelia and neuronal cells in multicellular organisms, similar mechanisms are ...
journal_title：Protoplasma
pub_type： 杂志文章,评审
doi：10.1007/s00709-017-1075-2
更新日期：2017-05-01 00:00:00
abstract：:In the present study energy-filtering transmission electron microscopy by use of an in-column spectrometer is employed as a powerful tool for ultrastructural analysis of plant cells. Images of unstained very thin (50 nm) and thick (140 nm) sections of the unicellular green alga Micrasterias denticulata, as a model sys...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-003-0033-3
更新日期：2004-06-01 00:00:00
abstract：:The aim of this work was to study the activity of NAD(P)H:(quinone acceptor) oxidoreductase 1 (EC 1.6.99.2) in the regeneration of lipophilic antioxidants, alpha-tocopherol, and reduced-coenzyme Q analogs. First, we tested whether or not two isoforms of the NAD(P)H:(quinone acceptor) oxidoreductase 1 designated as "hy...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-002-0068-x
更新日期：2003-05-01 00:00:00
abstract：:Puccinia striiformis f. sp. hordei, the causal organism of stripe rust in barley poses serious threats to its production. The present study examined the seedling response and changes in antioxidant defence system along with NADPH oxidase, hydrogen peroxide, and lipid peroxidation marker-malondialdehyde (MDA) in the fo...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-019-01428-5
更新日期：2020-01-01 00:00:00
abstract：:Here we present an overview of what is known about endogenous plant compounds that act as inhibitors of hormonal transport processes in plants, about their identity and mechanism of action. We have also summarized commonly and less commonly used compounds of non-plant origin and synthetic drugs that show at least part...
journal_title：Protoplasma
pub_type： 杂志文章,评审
doi：10.1007/s00709-015-0897-z
更新日期：2016-11-01 00:00:00
abstract：:Syagrus coronata is an economically important palm tree grown as an ornament, for the oil extracted from its seeds, and the wax from its leaves which has several applications in industry. Silicon biocomposites were analyzed in leaves of S. coronata. Silica bodies were found as extracellular silica masses between the h...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-002-0036-5
更新日期：2002-10-01 00:00:00
abstract：:While there is a number of studies on the effects of Rho GTPases on the actin-based cytoskeleton in higher eukaryotes, studies in protozoans are rather limited. The problem seems to be intriguing since the structure of protozoan cytoskeletons is distinct from most vertebrate cells. By blocking endogenous Rho family pr...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-002-0040-9
更新日期：2003-03-01 00:00:00
abstract：:The mature apomictic embryo sac of Chondrilla juncea is highly vacuolated and demonstrates a polarization similar to that of the amphimictic gametophyte. The microtubule cytoskeleton of this embryo sac is uncharacteristic and relatively weak. The microtubules are positioned along cell walls and resemble cortical micro...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-006-0147-5
更新日期：2006-05-01 00:00:00
abstract：:Narcissus tazetta is one of the major geophyte crops worldwide, but little is known about its cell biology. The narcissus storage organ was studied by monitoring scale cell biology during the growth stage and dormancy, and it was found that amyloplasts gradually increased in size and reached a maximum at dormancy. In ...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-006-0238-3
更新日期：2007-01-01 00:00:00
abstract：:The present study investigated the impact of 1800-MHz electromagnetic field radiations (EMF-r), widely used in mobile communication, on the growth and activity of starch-, sucrose-, and phosphate-hydrolyzing enzymes in Zea mays seedlings. We exposed Z. mays to modulated continuous wave homogenous EMF-r at specific abs...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-015-0863-9
更新日期：2016-07-01 00:00:00
abstract：:In order to gain more insight into the involvement of mitochondrial complex III in the Cd-induced stress, we studied the effect of complex III inhibitors, antimycin A (AA), and myxothiazol (MYXO), on the Cd-induced ROS and NO generation in the barley root tip. Short-term exposure of barley roots to either MYXO or AA p...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-019-01389-9
更新日期：2019-09-01 00:00:00
abstract：:In addition to small molecules such as sugars and amino acids, phloem sap contains macromolecules, including mRNA and proteins. It is generally assumed that all molecules in the phloem sap are on the move from source to sink, but recent evidence suggests that the macromolecules' direction of movement can be controlled...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-010-0215-8
更新日期：2011-01-01 00:00:00
abstract：:This paper reports an efficient protoplast culture technique, the "extra thin alginate film" technique. The development of this improved method of protoplast culture was an outcome of an assessment of the efficiency and shortcomings of various protoplast culture techniques. The efficiency of this technique was evaluat...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-005-0096-4
更新日期：2005-12-01 00:00:00
abstract：:Isolated microspores of B. napus in culture change their developmental pathway from gametophytic to sporophytic and form embryo-like structures (ELS) upon prolonged heat shock treatment (5 days at 32 °C). ELS express polarity during the initial days of endosporic development. In this study, we focussed on the analysis...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-013-0530-y
更新日期：2014-01-01 00:00:00
abstract：:The distribution and development of secretory cells and crystal cells in young shoot apexes of water hyacinth were investigated through morphological and cytological analysis. The density of secretory cells and crystal cells were high in parenchyma tissues around the vascular bundles of shoot apexes. Three development...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-010-0157-1
更新日期：2011-04-01 00:00:00
abstract：:The extent to which the actomyosin motor responsible for cytoplasmic streaming contributes to the translational diffusion of water in Elodea canadensis cells was studied by a nuclear magnetic resonance (NMR) spin-echo technique. The relative contribution of the actomyosin motor was determined from the corresponding ap...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-004-0067-1
更新日期：2004-12-01 00:00:00
abstract：:In the present investigation, we have used adenosine triphosphatase (ATPase) activity as biochemical test of toxic action of lindane that was explained by lipid peroxidation model. Study was also undertaken to ascertain the potential protective role of alpha-lipoic acid (ALA) and vitamin E on the same parameters. High...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-010-0121-0
更新日期：2010-06-01 00:00:00
abstract：:Drought tolerance is a complex trait that is governed by multiple genes. The study presents differential transcriptome analysis between drought-tolerant (Triticum aestivum Cv. C306) and drought-sensitive (Triticum aestivum Cv. WL711) genotypes, using Affymetrix GeneChip® Wheat Genome Array. Both genotypes exhibited di...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-018-1237-x
更新日期：2018-09-01 00:00:00
