Abstract:
:The aim of this work was to study the activity of NAD(P)H:(quinone acceptor) oxidoreductase 1 (EC 1.6.99.2) in the regeneration of lipophilic antioxidants, alpha-tocopherol, and reduced-coenzyme Q analogs. First, we tested whether or not two isoforms of the NAD(P)H:(quinone acceptor) oxidoreductase 1 designated as "hydrophilic" and "hydrophobic" (H. J. Prochaska and P. Talalay, Journal of Biological Chemistry 261: 1372-1378, 1986) show differential enzyme activities towards hydrophilic or hydrophobic ubiquinone homologs. By chromatography on phenyl Sepharose, we purified the two isoforms from pig liver cytosol and measured their reduction of several ubiquinone homologs of different side chain length. We also studied by electron paramagnetic resonance the effect of NAD(P)H:(quinone acceptor) oxidoreductase 1 on steady-state levels of chromanoxyl radicals generated by linoleic acid and lipooxygenase and confirmed the enzyme's ability to protect alpha-tocopherol against oxidation induced with H(2)O(2)-Fe(2+). Our results demonstrated that the different hydrophobicities of the isoforms do not reflect different reactivities towards ubiquinones of different side chain length. In addition, electron paramagnetic resonance studies showed that in systems containing the reductase plus NADH, levels of chromanoxyl radicals were dramatically reduced. Morever, in the presence of oxidants, alpha-tocopherol was preserved by NAD(P)H:(quinone acceptor) oxidoreductase 1, supporting our hypothesis that regeneration of alpha-tocopherol may be one of the physiologic functions of this enzyme.
journal_name
Protoplasmajournal_title
Protoplasmaauthors
Bello RI,Kagan VE,Tyurin V,Navarro F,Alcaín FJ,Villalba JMdoi
10.1007/s00709-002-0068-xsubject
Has Abstractpub_date
2003-05-01 00:00:00pages
129-35issue
1-2eissn
0033-183Xissn
1615-6102journal_volume
221pub_type
杂志文章相关文献
PROTOPLASMA文献大全abstract::We used an ultraviolet microbeam to cut individual kinetochore spindle fibres in metaphase crane-fly spermatocytes. We then followed the growth of the "kinetochore stubs", the remnants of kinetochore fibres that remain attached to kinetochores. Kinetochore stubs elongate with constant velocity by adding tubulin subuni...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-007-0265-8
更新日期:2007-01-01 00:00:00
abstract::Abiotic stresses, especially salinity and drought, are major limiting factors for plant growth and crop productivity. In an attempt to develop salt and drought tolerant tomato, a DNA cassette containing tobacco osmotin gene driven by a cauliflower mosaic virus 35S promoter was transferred to tomato (Solanum lycopersic...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-010-0158-0
更新日期:2010-09-01 00:00:00
abstract::Cell walls and protoplast may work together or distinctly in the establishment of the functional profiles of gall tissue compartments. This presumption is herein evaluated in three gall systems by immunocytochemical and ultrastructural analyses. The common storage tissues (CSTs) of leaf galls induced by Eriophyidae on...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-019-01431-w
更新日期:2020-01-01 00:00:00
abstract::Marcetia taxifolia (A. St.-Hil.) DC. hosts two gall morphotypes, a pistil-shaped gall induced by a Cecidomyiidae (Diptera) and a fusiform stem gall induced by a Lepidoptera. The cytological study of these galls aimed to answer how the difference in nutritive tissues of Diptera and Lepidoptera galls could be explained ...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-015-0759-8
更新日期:2015-09-01 00:00:00
abstract::Ripening which leads to fruit senescence is an inimitable process characterized by vivid changes in color, texture, flavor, and aroma of the fleshy fruits. Our understanding of the mechanisms underlying the regulation of fruit ripening and senescence is far from complete. Molecular and biochemical studies on tomato (S...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-015-0836-z
更新日期:2016-03-01 00:00:00
abstract::The distribution and development of secretory cells and crystal cells in young shoot apexes of water hyacinth were investigated through morphological and cytological analysis. The density of secretory cells and crystal cells were high in parenchyma tissues around the vascular bundles of shoot apexes. Three development...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-010-0157-1
更新日期:2011-04-01 00:00:00
abstract::The cryptomonad nucleomorph is a vestigial nucleus of a eukaryotic red alga engulfed by a phagotrophic protist and retained as a photosynthetic endosymbiont. This review recounts the initial discovery and subsequent characterisation of the cryptomonad nucleomorph focusing on the key role of Peter Sitte and his protégé...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-017-1153-5
更新日期:2017-09-01 00:00:00
abstract::The present study investigated the impact of 1800-MHz electromagnetic field radiations (EMF-r), widely used in mobile communication, on the growth and activity of starch-, sucrose-, and phosphate-hydrolyzing enzymes in Zea mays seedlings. We exposed Z. mays to modulated continuous wave homogenous EMF-r at specific abs...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-015-0863-9
更新日期:2016-07-01 00:00:00
abstract::The LIM domain is an evolutionary conserved double-zinc finger motif found in a variety of proteins exhibiting diverse biological roles. LIM domains have been observed to act as modular protein-binding interfaces mediating protein-protein interactions in the cytoplasm and the nucleus. Interaction of LIM domains with s...
journal_title:Protoplasma
pub_type: 杂志文章,评审
doi:10.1007/s007090200000
更新日期:2002-02-01 00:00:00
abstract::Approximately 20,000 mammalian genes are estimated to encode between 250 thousand and 1 million different proteins. This enormous diversity of the mammalian proteome is caused by the ability of a single-gene locus to encode multiple protein isoforms. Protein isoforms encoded by one gene locus can be functionally disti...
journal_title:Protoplasma
pub_type: 杂志文章,评审
doi:10.1007/s00709-016-1071-y
更新日期:2017-05-01 00:00:00
abstract::Fibrillarin and the plant nucleolin homolog NopA64 are two important nucleolar proteins involved in pre-rRNA processing. In order to determine the effects of the altered gravity environment on the nucleolus, we have investigated the location of fibrillarin and NopA64 in nucleolar subcomponents of cress (Lepidium sativ...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-006-0157-3
更新日期:2006-09-01 00:00:00
abstract::In order to corroborate the hypothesis that variations in the rate of spontaneous ultra-weak photon emission (UPE) from germinating seedlings are related to local variations of the lunisolar tidal force, a series of simultaneous tests was performed using the time courses of UPE collected from three plant species-corn,...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-016-0947-1
更新日期:2017-01-01 00:00:00
abstract::Lichen secondary metabolites can function as allelochemicals and affect the development and growth of neighboring bryophytes, fungi, vascular plants, microorganisms, and even other lichens. Lichen overgrowth on bryophytes is frequently observed in nature even though mosses grow faster than lichens, but there is still ...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-016-1022-7
更新日期:2017-05-01 00:00:00
abstract::In the vascular plant Arabidopsis thaliana, synthesis of cysteine and its precursors O-acetylserine and sulfide is distributed between the cytosol, chloroplasts, and mitochondria. This compartmentation contributes to regulation of cysteine synthesis. In contrast to Arabidopsis, cysteine synthesis is exclusively restri...
journal_title:Protoplasma
pub_type: 杂志文章,评审
doi:10.1007/s00709-012-0411-9
更新日期:2012-06-01 00:00:00
abstract::Using higher-resolution wide-field computational optical-sectioning fluorescence microscopy, the distribution of antigens recognized by antibodies against animal beta 1 integrin, fibronectin, and vitronectin has been visualized at the outer surface of enzymatically protoplasted onion epidermis cells and in depectinate...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/BF01882029
更新日期:1996-01-01 00:00:00
abstract::HLA-B associated transcript 1 (BAT1) protein, also named as spliceosome RNA helicase UAP56, is a member of the DExD/H-box family of helicases. However, regulation under stress, biochemical properties, and functions of plant homologue of BAT1 are poorly understood. Here, we report the purification and detailed biochemi...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-015-0791-8
更新日期:2015-11-01 00:00:00
abstract::Here, we describe the development of chloroplasts and the buildup of the thylakoid membranes in growing Arabidopsis leaves. Organelles were analyzed from three distinct positions, namely, at the tip, the upper leaf margin, and the base from leaves 1, 3, 5, and 7 of 14-day-old plants. Clear developmental gradients are ...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-016-1057-9
更新日期:2017-09-01 00:00:00
abstract::Autophagy is an important mechanism for recycling cell materials upon encountering stress conditions. Our previous studies had shown that TMV infection could lead to systemic PCD in the distal uninfected tissues, including root tip and shoot tip tissues. But it is not clear whether there is autophagy in the distal api...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-017-1125-9
更新日期:2018-01-01 00:00:00
abstract::Sugarcane shows reduced crop stand under relatively suboptimal conditions; the main reason for this is its sensitivity to ionic stress in the soil solution. This research was performed to explore some physiological and developmental changes in the immature sugarcane buds submitted to salt stress and possible role of g...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-015-0841-2
更新日期:2016-05-01 00:00:00
abstract::The presence of specific protein types in claws and beaks of turtles is poorly known. The present immunological study describes the localization of some of the main corneous beta proteins (CBPs) coded in the epidermal differentiation complex of turtles. Three antibodies here utilized revealed that glycine-, cysteine-,...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-020-01494-0
更新日期:2020-07-01 00:00:00
abstract::The couplons of the cardiomyocyte form nanospaces within the cell that place the L-type calcium channel (Ca(v)1.2), situated on the plasmalemma, in opposition to the type 2 ryanodine receptor (RyR2), situated on the sarcoplasmic reticulum. These two molecules, which form the basis of excitation-contraction coupling, a...
journal_title:Protoplasma
pub_type: 杂志文章,评审
doi:10.1007/s00709-011-0347-5
更新日期:2012-02-01 00:00:00
abstract::The inflorescence of Hedychium coccineum Smith is thyrse, and the primary bracts are initiated in a spiral phyllotactic pattern on the sides of the inflorescence dome. Cincinnus primordia are initiated on the flank of the inflorescence apex, in the axils of primary bracts. This primordium subsequently develops a bract...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-010-0145-5
更新日期:2010-11-01 00:00:00
abstract::The life of plants growing in cold extreme environments has been well investigated in terms of morphological, anatomical, and ecophysiological adaptations. In contrast, long-term cellular or metabolic studies have been performed by only a few groups. Moreover, a number of single reports exist, which often represent ju...
journal_title:Protoplasma
pub_type: 杂志文章,评审
doi:10.1007/s00709-010-0161-5
更新日期:2010-08-01 00:00:00
abstract::Kinesin superfamily proteins are important microtubule-based motor proteins with a kinesin motor domain that is conserved among all eukaryotic organisms. They are responsible for unidirectionally transporting various cargoes, including membranous organelles, protein complexes, and mRNAs. They also play critical roles ...
journal_title:Protoplasma
pub_type: 杂志文章,评审
doi:10.1007/s00709-011-0357-3
更新日期:2012-06-01 00:00:00
abstract::The present study reports the Agrobacterium rhizogenes-mediated hairy root induction in of an ethno-medicinally significant herb-Boerhaavia diffusa L., for elucidating the underlying competence regarding its biosynthetic (i.e. boeravinone B and eupalitin) and bioactivity (antibacterial, antioxidant and anti-inflammato...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-015-0875-5
更新日期:2016-07-01 00:00:00
abstract::Cytoplasmic streaming is essential for intracellular communications but its specific functions are not well known. In Chara corallina internodes, long-distance interactions mediated by cyclosis are clearly evident with microscopy-pulse amplitude modulation (PAM) fluorometer under application of localized light (LL) pu...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-016-0975-x
更新日期:2017-01-01 00:00:00
abstract::Characean internodal cells develop alternating patterns of acid and alkaline zones along their surface in order to facilitate uptake of carbon required for photosynthesis. In this study, we used a pH-indicating membrane dye, 4-heptadecylumbiliferone, to study the kinetics of alkaline band formation and decomposition. ...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-019-01392-0
更新日期:2019-11-01 00:00:00
abstract::Yr36 is an important gene conferring resistance to stripe rust of wheat caused by Puccinia striiformis f. sp. tritici (Pst). To determine if the Yr36 resistance is correlated to reactive oxygen species (ROS) burst and cell death, wheat near-isogenic lines with (UC1041 + Yr36) and without (UC1041) the gene were histolo...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-015-0833-2
更新日期:2016-05-01 00:00:00
abstract::Cytomixis is a poorly studied process of nuclear migration between plant cells, discovered in microsporogenesis of several hundreds of plant species. The chromosomes that migrate between tobacco microsporocytes have been for the first time identified using fluorescence in situ hybridization (FISH), and the question wh...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-016-1028-1
更新日期:2017-05-01 00:00:00
abstract::Indeterminate growth and the production of new organs in plants require a constant supply of new cells. The majority of these cells are produced in mitotic regions called meristems. For primary or tip growth of the roots and shoots, the meristems are located in the apices. These apical meristems have been shown to fun...
journal_title:Protoplasma
pub_type: 杂志文章,评审
doi:10.1007/s00709-010-0211-z
更新日期:2010-12-01 00:00:00