Chloramphenicol acetyltransferase-a new selectable marker in stable nuclear transformation of the red alga Cyanidioschyzon merolae.

abstract：:Palaeontologically, eubacteria are > 3× older than neomura (eukaryotes, archaebacteria). Cell biology contrasts ancestral eubacterial murein peptidoglycan walls and derived neomuran N-linked glycoprotein coats/walls. Misinterpreting long stems connecting clade neomura to eubacteria on ribosomal sequence trees (plus mi...

journal_title：Protoplasma

authors： Cavalier-Smith T,Chao EE

更新日期：2020-05-01 00:00:00

abstract：:The structure of the digestive gland epithelium of a terrestrial isopod Porcellio scaber has been investigated by conventional scanning electron microscopy (SEM), focused ion beam-scanning electron microscopy (FIB/SEM), and light microscopy in order to provide evidence on morphology of the gland epithelial surface in ...

journal_title：Protoplasma

authors： Millaku A,Leser V,Drobne D,Godec M,Torkar M,Jenko M,Milani M,Tatti F

更新日期：2010-05-01 00:00:00

abstract：:Genetically encoded biosensors are increasingly used in visualising signalling processes in different organisms. Sensors based on green fluorescent protein technology are providing a great opportunity for using Förster resonance energy transfer (FRET) as a tool that allows for monitoring dynamic processes in living ce...

journal_title：Protoplasma

authors： Hamers D,van Voorst Vader L,Borst JW,Goedhart J

更新日期：2014-03-01 00:00:00

abstract：:The couplons of the cardiomyocyte form nanospaces within the cell that place the L-type calcium channel (Ca(v)1.2), situated on the plasmalemma, in opposition to the type 2 ryanodine receptor (RyR2), situated on the sarcoplasmic reticulum. These two molecules, which form the basis of excitation-contraction coupling, a...

journal_title：Protoplasma

authors： Asghari P,Scriven DR,Hoskins J,Fameli N,van Breemen C,Moore ED

更新日期：2012-02-01 00:00:00

abstract：:The effect of cytochalasin B (CB; 25 micrograms ml-1 in 1% dimethylsulfoxide, DMSO) upon the structural polarity of statocytes in cress roots is demonstrated. If normal, vertically grown roots are incubated in CB, the structural polarity of the statocytes is altered according to the developmental stage of the root. St...

journal_title：Protoplasma

authors： Hensel W

更新日期：1985-01-01 00:00:00

abstract：:Agrobacterium-mediated transformation of chickpea (Cicer arietinum L.) has been performed using cotyledonary node explants (CNs), which release phenolics upon excision that are detrimental to the viability of Agrobacterium tumefaciens and result in low transformation frequency. Twelve low molecular weight phenolic com...

journal_title：Protoplasma

authors： Yadav R,Mehrotra M,Singh AK,Niranjan A,Singh R,Sanyal I,Lehri A,Pande V,Amla DV

更新日期：2017-01-01 00:00:00

abstract：:The present investigation analyzes the in vitro P solubilization [Ca-P, Al-P, Fe(II)-P, and Fe(III)-P] efficiency of native PSB strains from acid soils of Odisha and exploitation of the same through biofertilization in peanut (Arachis hypogaea L.) growth and P acquisition. One hundred six numbers of soil samples with ...

journal_title：Protoplasma

authors： Pradhan M,Sahoo RK,Pradhan C,Tuteja N,Mohanty S

更新日期：2017-11-01 00:00:00

abstract：:The present study reports the Agrobacterium rhizogenes-mediated hairy root induction in of an ethno-medicinally significant herb-Boerhaavia diffusa L., for elucidating the underlying competence regarding its biosynthetic (i.e. boeravinone B and eupalitin) and bioactivity (antibacterial, antioxidant and anti-inflammato...

journal_title：Protoplasma

authors： Gupta R,Pandey P,Singh S,Singh DK,Saxena A,Luqman S,Bawankule DU,Banerjee S

更新日期：2016-07-01 00:00:00

abstract：:The small GTPase CDC42 is ubiquitously expressed in eukaryotes, where it participates in the regulation of the cytoskeleton and a wide range of cellular processes, including cytokinesis, gene expression, cell cycle progression, apoptosis, and tumorigenesis. As very little is known on the molecular level about mycorrhi...

journal_title：Protoplasma

authors： Menotta M,Amicucci A,Basili G,Rivero F,Polidori E,Sisti D,Stocchi V

更新日期：2007-01-01 00:00:00

abstract：:In the grass snake (Natrix natrix), the newly developed somites form vesicles that are located on both sides of the neural tube. The walls of the vesicles are composed of tightly connected epithelial cells surrounding the cavity (the somitocoel). Also, in the newly formed somites, the Pax3 protein can be observed in t...

journal_title：Protoplasma

authors： Lewandowski D,Dubińska-Magiera M,Posyniak E,Rupik W,Daczewska M

更新日期：2017-07-01 00:00:00

abstract：:The effects of arsenite treatment on generation of reactive oxygen species, induction of oxidative stress, response of antioxidative system, and synthesis of phytochelatins were investigated in two indica rice (Oryza sativa L.) cvs. Malviya-36 and Pant-12 grown in sand cultures for a period of 5-20 days. Arsenite (As(...

journal_title：Protoplasma

authors： Mishra S,Jha AB,Dubey RS

更新日期：2011-07-01 00:00:00

abstract：:Intracellular molecular transport and localization are crucial for cells (plant cells as much as mammalian cells) to proliferate and to adapt to diverse environmental conditions. Here, some aspects of the microscopy-based method of fluorescence recovery after photobleaching (FRAP) are introduced. In the course of the ...

journal_title：Protoplasma

authors： Wachsmuth M

更新日期：2014-03-01 00:00:00

abstract：:Capitate glandular trichomes (CGT) of sunflower, Helianthus annuus, synthesize bioactive sesquiterpene lactones (STLs) within a short period of only a few days during trichome development. In the current project, the subcellular localization of H. annuus germacrene A monooxygenase (HaGAO), a key enzyme of the STL bios...

journal_title：Protoplasma

authors： Amrehn E,Aschenbrenner AK,Heller A,Spring O

更新日期：2016-03-01 00:00:00

abstract：:Endoplasmic reticulum (ER) immunolabeling in developing stomatal complexes and in the intervening cells of the stomatal rows (ICSRs) of Zea mays revealed that the cortical-ER forms distinct aggregations lining locally expanding wall regions. The polarized subsidiary cell mother cells (SMCs), displayed a cortical-ER-pa...

journal_title：Protoplasma

authors： Giannoutsou EP,Apostolakos P,Galatis B

更新日期：2011-04-01 00:00:00

abstract：:In a typical cell division, chromosomes align at the metaphase plate before anaphase commences. This is not the case in Mesostoma spermatocytes. Throughout prometaphase, the three bivalents persistently oscillate towards and away from either pole, at average speeds of 5-6 μm/min, without ever aligning at a metaphase p...

journal_title：Protoplasma

authors： Fegaras E,Forer A

更新日期：2018-07-01 00:00:00

abstract：:Determinations of plant or algal cell density (cell mass divided by volume) have rarely accounted for the extracellular matrix or shrinkage during isolation. Three techniques were used to indirectly estimate the density of intact apical cells from protonemata of the moss Ceratodon purpureus. First, the volume fraction...

journal_title：Protoplasma

authors： Schwuchow JM,Kern VD,Wagner T,Sack FD

更新日期：2000-01-01 00:00:00

abstract：:Pongamia pinnata L. is a multipurpose versatile legume that is well known as a prospective feedstock biodiesel species. However, to date, there has been little genomic research aimed at the exploitation of the biotechnological potential of this species. Genetic characterization of any plant is a challenging task when ...

journal_title：Protoplasma

authors： Choudhury RR,Basak S,Ramesh AM,Rangan L

更新日期：2014-05-01 00:00:00

abstract：:Plant growth and development are intimately attuned to fluctuations in environmental variables such as light, temperature and water availability. A broad range of signalling and dynamic response mechanisms allows them to adjust their physiology so that growth and reproductive capacity are optimised for the prevailing ...

journal_title：Protoplasma

authors： Seung D,Risopatron JP,Jones BJ,Marc J

更新日期：2012-07-01 00:00:00

abstract：:Abiotic stresses, such as drought and high salinity, are major factors that limit plant growth and productivity. Late embryogenesis abundant (LEA) proteins are members of a diverse, multigene family closely associated with tolerance to abiotic stresses in numerous organisms. We examined the function of SmLEA2, previou...

journal_title：Protoplasma

authors： Wang H,Wu Y,Yang X,Guo X,Cao X

更新日期：2017-03-01 00:00:00

abstract：:Lichen secondary metabolites can function as allelochemicals and affect the development and growth of neighboring bryophytes, fungi, vascular plants, microorganisms, and even other lichens. Lichen overgrowth on bryophytes is frequently observed in nature even though mosses grow faster than lichens, but there is still ...

journal_title：Protoplasma

authors： Goga M,Antreich SJ,Bačkor M,Weckwerth W,Lang I

更新日期：2017-05-01 00:00:00

abstract：:Short-time temperature effects (34-40 °C) on microtubule (MT) organization and on cell structure of young epidermal leaf cells of the seagrass Cymodocea nodosa were investigated under laboratory conditions using transmission electron microscopy (TEM) and tubulin immunofluorescence. The interphase MT network was affect...

journal_title：Protoplasma

authors： Koutalianou M,Orfanidis S,Katsaros C

更新日期：2016-03-01 00:00:00

abstract：:The original version of this article unfortunately contains a mistake. ...

journal_title：Protoplasma

authors： Asada T

更新日期：2019-05-01 00:00:00

abstract：:Frost resistance of sprouting Picea abies shoots is insufficient for survival of naturally occurring late frosts. The cellular changes during sprouting appeared to be responsible for frost damage as frost events that damaged sprouting shoots did not damage older needles and stems. Whilst resting buds showed initial fr...

journal_title：Protoplasma

authors： Neuner G,Beikircher B

更新日期：2010-07-01 00:00:00

abstract：:In this study, tansy (Tanacetum vulgare L.) in vitro culture was established from seeds collected from natural populations. The multiplication of plantlets was conducted through shoot tips that exhibited potent apical growth and regeneration capacities on basal medium (BM), without the addition of any plant growth reg...

journal_title：Protoplasma

authors： Devrnja N,Krstić-Milošević D,Janošević D,Tešević V,Vinterhalter B,Savić J,Ćalić D

更新日期：2020-11-27 00:00:00

abstract：:Intestinal ischemia and reperfusion (I/R) causes cellular and tissue damage to the intestine and remote organs such as the liver. Increased production of ROS and nitric oxide and dysregulation of cytoprotective enzymes may be involved in intestinal I/R. The aim was to evaluate the protective effects of glutamine on th...

journal_title：Protoplasma

authors： Hartmann RM,Licks F,Schemitt EG,Colares JR,do Couto Soares M,Zabot GP,Fillmann HS,Marroni NP

更新日期：2017-11-01 00:00:00

abstract：:Chlamydomonas acidophila LAFIC-004 is an acidophilic strain of green microalgae isolated from coal mining drainage. In the present work, this strain was cultivated in acidic medium (pH 3.6) under phototrophic, mixotrophic, and heterotrophic regimes to determine the best condition for growth and lipid production, simul...

journal_title：Protoplasma

authors： Souza LD,Simioni C,Bouzon ZL,Schneider RC,Gressler P,Miotto MC,Rossi MJ,Rörig LR

更新日期：2017-05-01 00:00:00

abstract：:Many studies have been carried out to investigate the histological structure of the trachea in many species of birds. However, the cellular organization of the trachea in the mallard duck is still unclear. This study was performed on 12 sexually mature male Mallard duck to demonstrate the cellular organization of the ...

journal_title：Protoplasma

authors： Mokhtar DM,Hussien MM

更新日期：2020-03-01 00:00:00

abstract：:The wild grass species Brachypodium distachyon (L.) has been proposed as a new model for temperate grasses. Among the biotechnological tools already developed for the species, an efficient induction protocol of somatic embryogenesis (SE) using immature zygotic embryos has provided the basis for genetic transformation ...

journal_title：Protoplasma

authors： Oliveira EJ,Koehler AD,Rocha DI,Vieira LM,Pinheiro MVM,de Matos EM,da Cruz ACF,da Silva TCR,Tanaka FAO,Nogueira FTS,Otoni WC

更新日期：2017-09-01 00:00:00

abstract：:Through reverse transcription-polymerase chain reaction and Northern blot analysis, we identified TBK5, a novel plant-specific kinesin-like protein (KLP) that is highly expressed in interphase tobacco BY-2 cells. TBK5 mRNA was present at a high level throughout the growth cycle, even in cells that had entered the stat...

journal_title：Protoplasma

authors： Matsui K,Collings D,Asada T

更新日期：2001-01-01 00:00:00

abstract：:Understanding the mechanisms that microbes exploit to invade host cells and cause disease is crucial if we are to eliminate their threat. Although pathogens use a variety of microbial factors to trigger entry into non-phagocytic cells, their targeting of the host cell process of endocytosis has emerged as a common the...

journal_title：Protoplasma

authors： Lin AE,Guttman JA

更新日期：2010-08-01 00:00:00