Abstract:
:This article deals with the distribution of callose and of the homogalacturonan (HG) epitopes recognized by LM20, JIM5, and 2F4 antibodies in cell walls of differentiating and functioning stomatal complexes of the monocotyledon Zea mays and the dicotyledon Vigna sinensis. The findings revealed that, during stomatal development, in these plant species, callose appears in an accurately spatially and timely controlled manner in cell walls of the guard cells (GCs). In functioning stomata of both plants, callose constitutes a dominant cell wall matrix material of the polar ventral cell wall ends and of the local GC cell wall thickenings. In Zea mays, the LM20, JIM5, or 2F4 antibody-recognized HG epitopes were mainly located in the expanding cell wall regions of the stomatal complexes, while in Vigna sinensis, they were deposited in the local cell wall thickenings of the GCs as well as at the ledges of the stomatal pore. Consideration of the presented data favors the view that in the stomatal complexes of the monocotyledon Z. mays and the dicotyledon V. sinensis, the esterified HGs contribute to the cell wall expansion taking place during GC morphogenesis and the opening of the stomatal pore. Besides, callose and the highly de-esterified HGs allow to GC cell wall regions to withstand the mechanical stresses exerted during stomatal function.
journal_name
Protoplasmajournal_title
Protoplasmaauthors
Giannoutsou E,Sotiriou P,Nikolakopoulou TL,Galatis B,Apostolakos Pdoi
10.1007/s00709-019-01425-8subject
Has Abstractpub_date
2020-01-01 00:00:00pages
141-156issue
1eissn
0033-183Xissn
1615-6102pii
10.1007/s00709-019-01425-8journal_volume
257pub_type
杂志文章相关文献
PROTOPLASMA文献大全abstract::Genetically encoded biosensors are increasingly used in visualising signalling processes in different organisms. Sensors based on green fluorescent protein technology are providing a great opportunity for using Förster resonance energy transfer (FRET) as a tool that allows for monitoring dynamic processes in living ce...
journal_title:Protoplasma
pub_type: 杂志文章,评审
doi:10.1007/s00709-013-0590-z
更新日期:2014-03-01 00:00:00
abstract::Cytomixis is a poorly studied process of nuclear migration between plant cells, discovered in microsporogenesis of several hundreds of plant species. The chromosomes that migrate between tobacco microsporocytes have been for the first time identified using fluorescence in situ hybridization (FISH), and the question wh...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-016-1028-1
更新日期:2017-05-01 00:00:00
abstract::We used a noninvasive microprobe technique to record in substomatal cavities of barley leaves the apoplastic pH response to different stress situations. When K+ (or Na+) activity at the roots of intact plants was increased from 1 to 50 mM, the leaf apoplastic pH increased by 0.4 to 0.6 units within 8 to 12 min when st...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-005-0131-5
更新日期:2005-12-01 00:00:00
abstract::A survey is presented of the architecture of secondary wall ingrowths in transfer cells from various taxa based on scanning electron microscopy. Wall ingrowths are a distinguishing feature of transfer cells and serve to amplify the plasma membrane surface area available for solute transport. Morphologically, two categ...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s007090200021
更新日期:2002-05-01 00:00:00
abstract::The ultrastructure of the several micrometers thick cytoplasmic layer of the giant marine alga Valonia utricularis displays characteristics which are apparently linked with the capability of this alga to regulate turgor pressure. Transmission and scanning electron microscopy of cells prefixed in different ways, includ...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-003-0021-7
更新日期:2003-01-01 00:00:00
abstract::Chlamydomonas acidophila LAFIC-004 is an acidophilic strain of green microalgae isolated from coal mining drainage. In the present work, this strain was cultivated in acidic medium (pH 3.6) under phototrophic, mixotrophic, and heterotrophic regimes to determine the best condition for growth and lipid production, simul...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-016-1030-7
更新日期:2017-05-01 00:00:00
abstract::We used an ultraviolet microbeam to cut individual kinetochore spindle fibres in metaphase crane-fly spermatocytes. We then followed the growth of the "kinetochore stubs", the remnants of kinetochore fibres that remain attached to kinetochores. Kinetochore stubs elongate with constant velocity by adding tubulin subuni...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-007-0265-8
更新日期:2007-01-01 00:00:00
abstract::Catchweed bedstraw is famous for its ability to adhere to other objects due to the presence of numerous trichomes surrounding the stem and mericarps and on the surfaces of the leaves. These trichomes serve as an efficient vector for the movement of the propagules via animals. In this study, we examined the structure a...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-008-0006-7
更新日期:2008-11-01 00:00:00
abstract::Palaeontologically, eubacteria are > 3× older than neomura (eukaryotes, archaebacteria). Cell biology contrasts ancestral eubacterial murein peptidoglycan walls and derived neomuran N-linked glycoprotein coats/walls. Misinterpreting long stems connecting clade neomura to eubacteria on ribosomal sequence trees (plus mi...
journal_title:Protoplasma
pub_type: 杂志文章,评审
doi:10.1007/s00709-019-01442-7
更新日期:2020-05-01 00:00:00
abstract::While there is a number of studies on the effects of Rho GTPases on the actin-based cytoskeleton in higher eukaryotes, studies in protozoans are rather limited. The problem seems to be intriguing since the structure of protozoan cytoskeletons is distinct from most vertebrate cells. By blocking endogenous Rho family pr...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-002-0040-9
更新日期:2003-03-01 00:00:00
abstract::Developing flowers are important sinks in Arabidopsis thaliana. Their energy demand is covered by assimilates which are synthesized in source leaves and transported via the vasculature. Assimilates are unloaded either symplastically through plasmodesmata or apoplastically by specific transport proteins. Here we studie...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-010-0223-8
更新日期:2011-01-01 00:00:00
abstract::The present investigation analyzes the in vitro P solubilization [Ca-P, Al-P, Fe(II)-P, and Fe(III)-P] efficiency of native PSB strains from acid soils of Odisha and exploitation of the same through biofertilization in peanut (Arachis hypogaea L.) growth and P acquisition. One hundred six numbers of soil samples with ...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-017-1112-1
更新日期:2017-11-01 00:00:00
abstract::Superoxide dismutase (SOD) activities of the oomycete Phytophthora cinnamomi were examined. Five polypeptides with manganese superoxide dismutase (MnSOD) activity were found in mycelium growing in liquid culture with relative molecular weights ranging from approximately 25 to 100 kDa. Comparison with characterized avo...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-012-0464-9
更新日期:2013-06-01 00:00:00
abstract::The potential genotoxicity of Cu(2+) was investigated in Vicia faba and Pisum sativum seedlings in hydroponic culture conditions. Cu(2+) caused a dose-dependent increase in micronuclei frequencies in both plant models. Cytological analysis of root tips cells showed clastogenic and aneugenic effects of this heavy metal...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-008-0004-9
更新日期:2008-11-01 00:00:00
abstract::Fragile sites (FSs) in plants have been described for species like Lolium and other grasses. Whereas in humans FSs were shown to be involved in genome instabilities; the consequences of FSs expression in plants are not known yet. To evaluate whether FSs cause karyotype instabilities, we assessed the frequency of micro...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-016-0942-6
更新日期:2017-01-01 00:00:00
abstract::Hyperhydricity is a physiological disorder associated with oxidative stress. Reactive oxygen species (ROS) generation in plants is initiated by various enzymatic sources, including plasma membrane-localized nicotinamide adenine dinucleotide phosphate (NADPH) oxidases, cell wall-bound peroxidase (POD), and apoplastic p...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-016-0957-z
更新日期:2017-01-01 00:00:00
abstract::Various stresses such as starvation and cold or heat shocks have been identified as triggers in the induction of the microspore embryogenesis. This study attempts to quantify the effects of different pretreatment conditions for successful microspore culture of malting barley (cv. Scarlett). While the sporophytic micro...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-006-0179-x
更新日期:2006-08-01 00:00:00
abstract::In the present study energy-filtering transmission electron microscopy by use of an in-column spectrometer is employed as a powerful tool for ultrastructural analysis of plant cells. Images of unstained very thin (50 nm) and thick (140 nm) sections of the unicellular green alga Micrasterias denticulata, as a model sys...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-003-0033-3
更新日期:2004-06-01 00:00:00
abstract::The purpose of the main research was to investigate the effects of methyl jasmonate (MeJA) (0.05, 0.25, 0.5, and 2.5 mM) on the pollen germination and tube elongation of Pinus nigra. Total pollen germination rate increased after MeJA treatments while the most enhancement was observed at 0.05-mM MeJA. No germination wa...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-020-01539-4
更新日期:2020-11-01 00:00:00
abstract::In plants, glucose (Glc) acts as a crucial signaling molecule in mediating metabolism, growth, stress tolerance mechanism, etc. However, little is known about Glc supplementation in salinity tolerance. This experiment was designed to study the ameliorative effect of Glc in mustard under salt stress. The seeds were soa...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-020-01600-2
更新日期:2021-01-03 00:00:00
abstract::The distribution and development of secretory cells and crystal cells in young shoot apexes of water hyacinth were investigated through morphological and cytological analysis. The density of secretory cells and crystal cells were high in parenchyma tissues around the vascular bundles of shoot apexes. Three development...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-010-0157-1
更新日期:2011-04-01 00:00:00
abstract::Abiotic stresses, such as drought and high salinity, are major factors that limit plant growth and productivity. Late embryogenesis abundant (LEA) proteins are members of a diverse, multigene family closely associated with tolerance to abiotic stresses in numerous organisms. We examined the function of SmLEA2, previou...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-016-0981-z
更新日期:2017-03-01 00:00:00
abstract::Ascochyta pisi is a necrotrophic pathogenic fungus, which mainly survives between seasons through infected seeds. Defence responses of pea embryo axes to A. pisi were investigated in the heterotrophic phase of seed germination and during the transition from the heterotrophic to the autotrophic phase. Germinated pea se...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-012-0374-x
更新日期:2013-02-01 00:00:00
abstract::In the genus Genlisea as well as in its sister genus Utricularia, the palate probably plays a key role in providing the colour, mechanical and olfactory stimuli to attract insect pollinators and to guide them to the generative structures and the nectary spur. However, information about the micro-morphology of the pala...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-018-1220-6
更新日期:2018-07-01 00:00:00
abstract::The wild grass species Brachypodium distachyon (L.) has been proposed as a new model for temperate grasses. Among the biotechnological tools already developed for the species, an efficient induction protocol of somatic embryogenesis (SE) using immature zygotic embryos has provided the basis for genetic transformation ...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-017-1089-9
更新日期:2017-09-01 00:00:00
abstract::In this study, tansy (Tanacetum vulgare L.) in vitro culture was established from seeds collected from natural populations. The multiplication of plantlets was conducted through shoot tips that exhibited potent apical growth and regeneration capacities on basal medium (BM), without the addition of any plant growth reg...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-020-01588-9
更新日期:2020-11-27 00:00:00
abstract::Agrobacterium-mediated transformation of chickpea (Cicer arietinum L.) has been performed using cotyledonary node explants (CNs), which release phenolics upon excision that are detrimental to the viability of Agrobacterium tumefaciens and result in low transformation frequency. Twelve low molecular weight phenolic com...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-015-0940-0
更新日期:2017-01-01 00:00:00
abstract::The inflorescence of Hedychium coccineum Smith is thyrse, and the primary bracts are initiated in a spiral phyllotactic pattern on the sides of the inflorescence dome. Cincinnus primordia are initiated on the flank of the inflorescence apex, in the axils of primary bracts. This primordium subsequently develops a bract...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-010-0145-5
更新日期:2010-11-01 00:00:00
abstract::Radial oxygen loss is a physical phenomenon that occurs naturally in aquatic plants. Typha domingensis was chosen as a model plant because it possesses basic morphological characteristics, such as a stem (rhizome) that produces leaves and adventitious roots, which are present in many aquatic plants. This study aimed t...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-020-01597-8
更新日期:2021-01-06 00:00:00
abstract::Formation of intra-molecular disulfides and concomitant oxidative protein folding is essential for stability and catalytic function of many soluble and membrane-bound proteins in the endomembrane system, the mitochondrial inter-membrane space and the thylakoid lumen. Disulfide generation from free cysteines in nascent...
journal_title:Protoplasma
pub_type: 杂志文章,评审
doi:10.1007/s00709-012-0463-x
更新日期:2013-08-01 00:00:00