Abstract：
:Hyperhydricity is a physiological disorder associated with oxidative stress. Reactive oxygen species (ROS) generation in plants is initiated by various enzymatic sources, including plasma membrane-localized nicotinamide adenine dinucleotide phosphate (NADPH) oxidases, cell wall-bound peroxidase (POD), and apoplastic polyamine oxidase (PAO). The origin of the oxidative burst associated with hyperhydricity remains unknown. To investigate the role of NADPH oxidases, POD, and PAO in ROS production and hyperhydricity, exogenous hydrogen peroxide (H2O2) and inhibitors of each ROS-producing enzyme were applied to explore the mechanism of oxidative stress induction in garlic plantlets in vitro. A concentration of 1.5 mM H2O2 increased endogenous ROS production and hyperhydricity occurrence and enhanced the activities of NADPH oxidases, POD, and PAO. During the entire treatment period, NADPH oxidase activity increased continuously, whereas POD and PAO activities exhibited a transient increase and subsequently declined. Histochemical and cytochemical visualization demonstrated that specific inhibitors of each enzyme effectively suppressed ROS accumulation. Moreover, superoxide anion generation, H2O2 content, and hyperhydric shoot frequency in H2O2-stressed plantlets decreased significantly. The NADPH oxidase inhibitor was the most effective at suppressing superoxide anion production. The results suggested that NADPH oxidases, POD, and PAO were responsible for endogenous ROS induction. NADPH oxidase activation might play a pivotal role in the oxidative burst in garlic plantlets in vitro during hyperhydricity.
journal_name
Protoplasmajournal_title
Protoplasmaauthors
Tian J,Cheng Y,Kong X,Liu M,Jiang F,Wu Zdoi
10.1007/s00709-016-0957-zsubject
Has Abstractpub_date
2017-01-01 00:00:00pages
379-388issue
1eissn
0033-183Xissn
1615-6102pii
10.1007/s00709-016-0957-zjournal_volume
254pub_type
杂志文章相关文献
PROTOPLASMA文献大全abstract：:We used an ultraviolet microbeam to cut individual kinetochore spindle fibres in metaphase crane-fly spermatocytes. We then followed the growth of the "kinetochore stubs", the remnants of kinetochore fibres that remain attached to kinetochores. Kinetochore stubs elongate with constant velocity by adding tubulin subuni...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-007-0265-8
更新日期：2007-01-01 00:00:00
abstract：:Some species of Spirogyra form rosette-shaped or rod-shaped rhizoids in the terminal cell of the filaments. In the present study, we analyzed an involvement of microtubules (MTs) in rhizoid differentiation. Before rhizoid differentiation, cortical MTs were arranged transversely to the long axis of cylindrical cells, r...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-002-0078-8
更新日期：2003-06-01 00:00:00
abstract：:In this paper, two transgenic wheat lines, PC27 and PC51, containing the maize PEPC gene and its wild-type (WT) were used as experimental material to study the effects of high temperature on their photosynthetic physiological characteristics and metabolome. The results showed that transgenic wheat lines had higher pho...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-016-1010-y
更新日期：2017-03-01 00:00:00
abstract：:Two novel beta-expansin genes, TaEXPB1 and TaEXPB2, were isolated from wheat microspores by suppression subtractive hybridisation. Northern blot and reverse transcription PCR analyses showed that the expression of both genes was restricted to early stages of male gametophyte development (from microspores to immature p...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-006-0176-0
更新日期：2006-08-01 00:00:00
abstract：:Despite great efforts in tissue engineering of the ureter, urinary bladder, and urethra, further research is needed in order to improve the patient's quality of life and minimize the economic burden of different lower urinary tract disorders. The nanostructured titanium dioxide (TiO2) scaffolds have a wide range of cl...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-015-0896-0
更新日期：2016-11-01 00:00:00
abstract：:The effects of β-amino butyric acid (BABA) on abiotic stress tolerance potential of three Vigna radiata varieties were studied. The reduction in the growth of seedlings subjected to NaCl/polyethylene glycol (PEG) stress is alleviated by BABA seed priming, which also enhanced photosynthetic pigment content and photosyn...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-015-0804-7
更新日期：2016-03-01 00:00:00
abstract：:Plant development is regulated by numerous chemicals derived from a multitude of metabolic pathways. However, we know very little about the biological effects and functions of many of these metabolites in the cell. N-Acylethanolamines (NAEs) are a group of lipid mediators that play important roles in mammalian physiol...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-005-0124-4
更新日期：2005-12-01 00:00:00
abstract：:The present study investigated the impact of 1800-MHz electromagnetic field radiations (EMF-r), widely used in mobile communication, on the growth and activity of starch-, sucrose-, and phosphate-hydrolyzing enzymes in Zea mays seedlings. We exposed Z. mays to modulated continuous wave homogenous EMF-r at specific abs...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-015-0863-9
更新日期：2016-07-01 00:00:00
abstract：:The detergent Brij 58 has been introduced to reverse plasma membrane (PM) vesicles from the right-side-out to the inside-out form. The aim of the present work was to investigate the effect of Brij 58 on the formation of an ATP-dependent proton gradient and on the fluidity of the lipid phase of PM vesicles. PMs of corn...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/BF01289415
更新日期：2001-01-01 00:00:00
abstract：:The structural changes occurred in differentiating olive cotyledon cells into mesophyll cells are described. Using histological and immunocytological methods as well as microscopic observations, we showed that in the cells of mature embryo, large electron-dense proteins bodies (PBs) are surrounded by numerous oil bodi...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-010-0242-5
更新日期：2011-10-01 00:00:00
abstract：:Telocytes (TCs), a novel type of interstitial cells, are involved in tissue homeostasis maintenance. This study aimed to investigate TC presence in the interstitium of mouse testis. Additionally, inactivation of the G-coupled membrane estrogen receptor (GPER) in the testis was performed to obtain insight into TC funct...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-018-1305-2
更新日期：2019-03-01 00:00:00
abstract：:The subtribe Cajaninae of papilionoid legumes has a pantropical distribution and comprises approximately 490 species. These species have diversified throughout dry environments where there are high temperatures and strong light. The subtribe stands out because all its representatives have vesicular glands. In addition...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-018-1307-0
更新日期：2019-03-01 00:00:00
abstract：:We present a new Chlamydomonas reinhardtii flagellar mutant in which central pair projections are missing and the central pair microtubules are twisted along the length of the flagellum. We have named this mutant tcp1 for twisted central pair. Immunoblots using an antibody that recognizes the heavy chain of sea urchin...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-007-0273-8
更新日期：2007-01-01 00:00:00
abstract：:In this study, the filamentous green alga Zygogonium ericetorum (Zygnematales, Chlorophyta), collected at its natural habitat in the high alps, was investigated by light, scanning, and transmission electron microscopy. The field samples were separated into a moist fraction when wetted by splattering water of a nearby ...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-009-0048-5
更新日期：2010-07-01 00:00:00
abstract：:The ultrastructure of the several micrometers thick cytoplasmic layer of the giant marine alga Valonia utricularis displays characteristics which are apparently linked with the capability of this alga to regulate turgor pressure. Transmission and scanning electron microscopy of cells prefixed in different ways, includ...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-003-0021-7
更新日期：2003-01-01 00:00:00
abstract：:The plant root tip represents a fascinating model system for studying changes in Golgi stack architecture associated with the developmental progression of meristematic cells to gravity sensing columella cells, and finally to "young" and "old", polysaccharide-slime secreting peripheral cells. To this end we have used ...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/BF01322640
更新日期：1990-01-01 00:00:00
abstract：:Short-time temperature effects (34-40 °C) on microtubule (MT) organization and on cell structure of young epidermal leaf cells of the seagrass Cymodocea nodosa were investigated under laboratory conditions using transmission electron microscopy (TEM) and tubulin immunofluorescence. The interphase MT network was affect...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-015-0809-2
更新日期：2016-03-01 00:00:00
abstract：:Genetically encoded biosensors are increasingly used in visualising signalling processes in different organisms. Sensors based on green fluorescent protein technology are providing a great opportunity for using Förster resonance energy transfer (FRET) as a tool that allows for monitoring dynamic processes in living ce...
journal_title：Protoplasma
pub_type： 杂志文章,评审
doi：10.1007/s00709-013-0590-z
更新日期：2014-03-01 00:00:00
abstract：:Ascorbate has been related to the differentiation of several mesenchymal cells including haematopoietic cells. We have previously demonstrated that ascorbate enhances the activity of 1 alpha,25-dihydroxyvitamin D3 (1 alpha,25(OH)2D3) on monocytic differentiation of HL-60 cells. Here, we show that ascorbate-mediated mo...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/BF01289422
更新日期：2001-01-01 00:00:00
abstract：:Here we present an overview of what is known about endogenous plant compounds that act as inhibitors of hormonal transport processes in plants, about their identity and mechanism of action. We have also summarized commonly and less commonly used compounds of non-plant origin and synthetic drugs that show at least part...
journal_title：Protoplasma
pub_type： 杂志文章,评审
doi：10.1007/s00709-015-0897-z
更新日期：2016-11-01 00:00:00
abstract：:Mastigonemes, tripartite tubular hairs on the anterior flagellum of Phytophthora zoospores, are instrumental for disease dissemination to new host plants. A previous study showed that PnMas2 was part of the tubular shaft of Phytophthora parasitica mastigonemes. In the current study, genes encoding two related proteins...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-018-1314-1
更新日期：2019-03-01 00:00:00
abstract：:In the grass snake (Natrix natrix), the newly developed somites form vesicles that are located on both sides of the neural tube. The walls of the vesicles are composed of tightly connected epithelial cells surrounding the cavity (the somitocoel). Also, in the newly formed somites, the Pax3 protein can be observed in t...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-016-1040-5
更新日期：2017-07-01 00:00:00
abstract：:The aim of this work was to study the activity of NAD(P)H:(quinone acceptor) oxidoreductase 1 (EC 1.6.99.2) in the regeneration of lipophilic antioxidants, alpha-tocopherol, and reduced-coenzyme Q analogs. First, we tested whether or not two isoforms of the NAD(P)H:(quinone acceptor) oxidoreductase 1 designated as "hy...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-002-0068-x
更新日期：2003-05-01 00:00:00
abstract：:This micromorphological, chemical and ultrastructural study is a continuation of research conducted on the section Lepidorhiza. The Bulbophyllum echinolabium flowers comprised features that characterize a sapromyophilous syndrome, having large, glistening parts that emit an intense scent of rotten meat. The secretory ...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-019-01372-4
更新日期：2019-09-01 00:00:00
abstract：:Despite more than 130 years of research, phloem loading is far from being understood in gymnosperms. In part this is due to the special architecture of their leaves. They differ from angiosperm leaves among others by having a transfusion tissue between bundle sheath and the axial vascular elements. This article review...
journal_title：Protoplasma
pub_type： 杂志文章,评审
doi：10.1007/s00709-010-0239-0
更新日期：2011-01-01 00:00:00
abstract：:Various stresses such as starvation and cold or heat shocks have been identified as triggers in the induction of the microspore embryogenesis. This study attempts to quantify the effects of different pretreatment conditions for successful microspore culture of malting barley (cv. Scarlett). While the sporophytic micro...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-006-0179-x
更新日期：2006-08-01 00:00:00
abstract：:Through reverse transcription-polymerase chain reaction and Northern blot analysis, we identified TBK5, a novel plant-specific kinesin-like protein (KLP) that is highly expressed in interphase tobacco BY-2 cells. TBK5 mRNA was present at a high level throughout the growth cycle, even in cells that had entered the stat...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/BF01280307
更新日期：2001-01-01 00:00:00
abstract：:Chlamydomonas acidophila LAFIC-004 is an acidophilic strain of green microalgae isolated from coal mining drainage. In the present work, this strain was cultivated in acidic medium (pH 3.6) under phototrophic, mixotrophic, and heterotrophic regimes to determine the best condition for growth and lipid production, simul...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-016-1030-7
更新日期：2017-05-01 00:00:00
abstract：:ALTERED MERISTEM PROGRAM 1 (AMP1) encodes a putative glutamate-carboxypeptidase important for plant growth and development. In this study, by comparing the growth of Arabidopsis wild-type, amp1-10 and amp1-13 mutants, and AMP1-GFP/OX2- and AMP1-GFP/OX7-overexpressing seedlings in vitro and in soil, we uncover the role...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-019-01458-z
更新日期：2020-03-01 00:00:00
abstract：:Capitate glandular trichomes (CGT) of sunflower, Helianthus annuus, synthesize bioactive sesquiterpene lactones (STLs) within a short period of only a few days during trichome development. In the current project, the subcellular localization of H. annuus germacrene A monooxygenase (HaGAO), a key enzyme of the STL bios...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-015-0823-4
更新日期：2016-03-01 00:00:00
