Differential effects of two phospholipase D inhibitors, 1-butanol and N-acylethanolamine, on in vivo cytoskeletal organization and Arabidopsis seedling growth.

abstract：:Single-molecule Förster resonance energy transfer (smFRET) has emerged as a powerful tool for elucidating biological structure and mechanisms on the molecular level. Here, we focus on applications of smFRET to study interactions between DNA and enzymes such as DNA and RNA polymerases. SmFRET, used as a nanoscopic rule...

journal_title：Protoplasma

authors： Farooq S,Fijen C,Hohlbein J

更新日期：2014-03-01 00:00:00

abstract：:In the vascular plant Arabidopsis thaliana, synthesis of cysteine and its precursors O-acetylserine and sulfide is distributed between the cytosol, chloroplasts, and mitochondria. This compartmentation contributes to regulation of cysteine synthesis. In contrast to Arabidopsis, cysteine synthesis is exclusively restri...

journal_title：Protoplasma

authors： Birke H,Müller SJ,Rother M,Zimmer AD,Hoernstein SN,Wesenberg D,Wirtz M,Krauss GJ,Reski R,Hell R

更新日期：2012-06-01 00:00:00

abstract：:The structural changes occurred in differentiating olive cotyledon cells into mesophyll cells are described. Using histological and immunocytological methods as well as microscopic observations, we showed that in the cells of mature embryo, large electron-dense proteins bodies (PBs) are surrounded by numerous oil bodi...

journal_title：Protoplasma

authors： Zienkiewicz A,Jiménez-López JC,Zienkiewicz K,de Dios Alché J,Rodríguez-García MI

更新日期：2011-10-01 00:00:00

abstract：:ALTERED MERISTEM PROGRAM 1 (AMP1) encodes a putative glutamate-carboxypeptidase important for plant growth and development. In this study, by comparing the growth of Arabidopsis wild-type, amp1-10 and amp1-13 mutants, and AMP1-GFP/OX2- and AMP1-GFP/OX7-overexpressing seedlings in vitro and in soil, we uncover the role...

journal_title：Protoplasma

authors： López-García CM,Ruíz-Herrera LF,López-Bucio JS,Huerta-Venegas PI,Peña-Uribe CA,de la Cruz HR,López-Bucio J

更新日期：2020-03-01 00:00:00

abstract：:Through reverse transcription-polymerase chain reaction and Northern blot analysis, we identified TBK5, a novel plant-specific kinesin-like protein (KLP) that is highly expressed in interphase tobacco BY-2 cells. TBK5 mRNA was present at a high level throughout the growth cycle, even in cells that had entered the stat...

journal_title：Protoplasma

authors： Matsui K,Collings D,Asada T

更新日期：2001-01-01 00:00:00

abstract：:The effectiveness of 2,3-butanedione monoxime (BDM) as an inhibitor of plant myosins has been investigated. Three myosin-dependent motility phenomena in plants, namely cytoplasmic streaming in Chara corallina, light-dependent chloroplast repositioning in Elodea sp., and brefeldin A(BFA)-induced Golgi membrane dynamics...

journal_title：Protoplasma

authors： McCurdy DW

更新日期：1999-01-01 00:00:00

abstract：:Seed storage proteins (SSPs) have been studied for more than 250 years because of their nutritional value and their impact on the use of grain in food processing. More recently, the use of seeds for the production of recombinant proteins has rekindled interest in the behavior of SSPs and the question how they are able...

journal_title：Protoplasma

authors： Ibl V,Stoger E

更新日期：2012-04-01 00:00:00

abstract：:Cytoplasmic streaming is essential for intracellular communications but its specific functions are not well known. In Chara corallina internodes, long-distance interactions mediated by cyclosis are clearly evident with microscopy-pulse amplitude modulation (PAM) fluorometer under application of localized light (LL) pu...

journal_title：Protoplasma

authors： Bulychev AA,Komarova AV

更新日期：2017-01-01 00:00:00

abstract：:Microtubule dynamics were examined in live cells of the fungal plant pathogen Magnaporthe grisea transformed for constitutive expression of a fusion protein containing enhanced yellow-fluorescent protein and a Neurospora crassa benomyl-resistant allele of beta-tubulin. Transformants retained their ability to different...

journal_title：Protoplasma

authors： Czymmek KJ,Bourett TM,Shao Y,DeZwaan TM,Sweigard JA,Howard RJ

更新日期：2005-04-01 00:00:00

abstract：:Reactive carbonyl species methylglyoxal (MG) has always been regarded as a cytotoxic metabolite, but now is emerging to function as signal molecule in plants. However, whether MG can induce salt tolerance is elusive. In this study, treatment of wheat seeds with NaCl reduced seed germination, plant height, root length,...

journal_title：Protoplasma

authors： Li ZG,Duan XQ,Min X,Zhou ZH

更新日期：2017-09-01 00:00:00

abstract：:The structure of the digestive gland epithelium of a terrestrial isopod Porcellio scaber has been investigated by conventional scanning electron microscopy (SEM), focused ion beam-scanning electron microscopy (FIB/SEM), and light microscopy in order to provide evidence on morphology of the gland epithelial surface in ...

journal_title：Protoplasma

authors： Millaku A,Leser V,Drobne D,Godec M,Torkar M,Jenko M,Milani M,Tatti F

更新日期：2010-05-01 00:00:00

abstract：:Endoplasmic reticulum (ER) immunolabeling in developing stomatal complexes and in the intervening cells of the stomatal rows (ICSRs) of Zea mays revealed that the cortical-ER forms distinct aggregations lining locally expanding wall regions. The polarized subsidiary cell mother cells (SMCs), displayed a cortical-ER-pa...

journal_title：Protoplasma

authors： Giannoutsou EP,Apostolakos P,Galatis B

更新日期：2011-04-01 00:00:00

abstract：:Tradescantia virginiana leaf epidermal cells were plasmolysed by sequential treatment with 0.8 M and 0.3 M sucrose. Plasmolysis revealed adhesion of the plasma membrane to the cell wall at sites coinciding with cytoskeletal arrays involved in the polarisation of cells undergoing asymmetric divisions--cortical actin pa...

journal_title：Protoplasma

authors： Cleary AL

更新日期：2001-01-01 00:00:00

abstract：:Lichen secondary metabolites can function as allelochemicals and affect the development and growth of neighboring bryophytes, fungi, vascular plants, microorganisms, and even other lichens. Lichen overgrowth on bryophytes is frequently observed in nature even though mosses grow faster than lichens, but there is still ...

journal_title：Protoplasma

authors： Goga M,Antreich SJ,Bačkor M,Weckwerth W,Lang I

更新日期：2017-05-01 00:00:00

abstract：:Indirect immunofluorescence and confocal microscopy were used to study the nucleation and organization of microtubules during meiosis in two species of leafy liverworts, Cephalozia macrostachya and Telaranea longifolia. This is the first such study of sporogenesis in the largest group of liverworts important as living...

journal_title：Protoplasma

authors： Brown RC,Lemmon BE

更新日期：2009-10-01 00:00:00

abstract：:We used a noninvasive microprobe technique to record in substomatal cavities of barley leaves the apoplastic pH response to different stress situations. When K+ (or Na+) activity at the roots of intact plants was increased from 1 to 50 mM, the leaf apoplastic pH increased by 0.4 to 0.6 units within 8 to 12 min when st...

journal_title：Protoplasma

authors： Felle HH,Herrmann A,Hückelhoven R,Kogel KH

更新日期：2005-12-01 00:00:00

abstract：:A comparative analysis of fluorochrome-binding pattern in nine taxa of Abelmoschus had shown that the type, amount and distribution pattern of heterochromatin were characteristic for each taxa. The fluorescent chromosome-binding sites obtained by chromomycin A3 (CMA) and 4',6-diamidino-2-phenylindole (DAPI) staining i...

journal_title：Protoplasma

authors： Merita K,Kattukunnel JJ,Yadav SR,Bhat KV,Rao SR

更新日期：2015-03-01 00:00:00

abstract：:Pongamia pinnata L. is a multipurpose versatile legume that is well known as a prospective feedstock biodiesel species. However, to date, there has been little genomic research aimed at the exploitation of the biotechnological potential of this species. Genetic characterization of any plant is a challenging task when ...

journal_title：Protoplasma

authors： Choudhury RR,Basak S,Ramesh AM,Rangan L

更新日期：2014-05-01 00:00:00

abstract：:The paper reports studies, including histological and ultrastructural analyses, of in vitro cell proliferation and development of immature endosperm tissue isolated from caryopses of Triticum aestivum, Triticum durum, and Triticosecale plants. Endosperm isolated at 7-10 days post-anthesis developed well on MS medium s...

journal_title：Protoplasma

authors： Popielarska-Konieczna M,Kozieradzka-Kiszkurno M,Tuleja M,Ślesak H,Kapusta P,Marcińska I,Bohdanowicz J

更新日期：2013-02-01 00:00:00

abstract：:In the grass snake (Natrix natrix), the newly developed somites form vesicles that are located on both sides of the neural tube. The walls of the vesicles are composed of tightly connected epithelial cells surrounding the cavity (the somitocoel). Also, in the newly formed somites, the Pax3 protein can be observed in t...

journal_title：Protoplasma

authors： Lewandowski D,Dubińska-Magiera M,Posyniak E,Rupik W,Daczewska M

更新日期：2017-07-01 00:00:00

abstract：:We have shown previously that the durum wheat TdSOS1 excludes Na+ and Li+ ions outside cells. Moreover, this protein is activated by Arabidopsis kinase SOS2 through phosphorylation. The elimination of both SOS2 phosphorylation sites and the auto-inhibitory domain produces a hyperactive TdSOS1∆972 form, which have a ma...

journal_title：Protoplasma

authors： Feki K,Tounsi S,Masmoudi K,Brini F

更新日期：2017-07-01 00:00:00

abstract：:The detergent Brij 58 has been introduced to reverse plasma membrane (PM) vesicles from the right-side-out to the inside-out form. The aim of the present work was to investigate the effect of Brij 58 on the formation of an ATP-dependent proton gradient and on the fluidity of the lipid phase of PM vesicles. PMs of corn...

journal_title：Protoplasma

authors： Behzadipour M,Kluge M,Lüthje S

更新日期：2001-01-01 00:00:00

abstract：:In this paper, we investigated whether nitric oxide (NO) participated in the regulation of the ascorbate-glutathione (AsA-GSH) cycle by exogenous jasmonic acid (JA) in the leaves of wheat seedlings under drought stress. The findings of our study showed that drought stress significantly enhanced the AsA-GSH cycle by up...

journal_title：Protoplasma

authors： Shan C,Zhou Y,Liu M

更新日期：2015-09-01 00:00:00

abstract：:While there is a number of studies on the effects of Rho GTPases on the actin-based cytoskeleton in higher eukaryotes, studies in protozoans are rather limited. The problem seems to be intriguing since the structure of protozoan cytoskeletons is distinct from most vertebrate cells. By blocking endogenous Rho family pr...

journal_title：Protoplasma

authors： Kłopocka W,Redowicz MJ

更新日期：2003-03-01 00:00:00

abstract：:The present investigation analyzes the in vitro P solubilization [Ca-P, Al-P, Fe(II)-P, and Fe(III)-P] efficiency of native PSB strains from acid soils of Odisha and exploitation of the same through biofertilization in peanut (Arachis hypogaea L.) growth and P acquisition. One hundred six numbers of soil samples with ...

journal_title：Protoplasma

authors： Pradhan M,Sahoo RK,Pradhan C,Tuteja N,Mohanty S

更新日期：2017-11-01 00:00:00

abstract：:Wheat contains the largest number of miR396 family with 17 miR396 in Poaceae. MiR396 regulatory network underlying wheat grain development has not comprehensively been explored. Our results showed that precursor miR396 family in Poaceae exhibited not only conservativeness but also diversification especially in wheat. ...

journal_title：Protoplasma

authors： Yu Y,Sun F,Chen N,Sun G,Wang CY,Wu DX

更新日期：2021-01-01 00:00:00

abstract：:The wild grass species Brachypodium distachyon (L.) has been proposed as a new model for temperate grasses. Among the biotechnological tools already developed for the species, an efficient induction protocol of somatic embryogenesis (SE) using immature zygotic embryos has provided the basis for genetic transformation ...

journal_title：Protoplasma

authors： Oliveira EJ,Koehler AD,Rocha DI,Vieira LM,Pinheiro MVM,de Matos EM,da Cruz ACF,da Silva TCR,Tanaka FAO,Nogueira FTS,Otoni WC

更新日期：2017-09-01 00:00:00

abstract：:In the genus Genlisea as well as in its sister genus Utricularia, the palate probably plays a key role in providing the colour, mechanical and olfactory stimuli to attract insect pollinators and to guide them to the generative structures and the nectary spur. However, information about the micro-morphology of the pala...

journal_title：Protoplasma

authors： Płachno BJ,Świątek P,Stpiczyńska M,Miranda VFO

更新日期：2018-07-01 00:00:00

abstract：:Pollen developmental pathway in plants involving synchronized transferal of cellular divisions from meiosis (microsporogenesis) to mitosis (pollen mitosis I/II) eventually offers a unique "meiosis-mitosis shift" at pollen mitosis I. Since the cell type (haploid microspore) and fate of pollen mitosis I differ from typi...

journal_title：Protoplasma

authors： Sharma SK,Yamamoto M,Mukai Y

更新日期：2017-01-01 00:00:00

abstract：:The cryptomonad nucleomorph is a vestigial nucleus of a eukaryotic red alga engulfed by a phagotrophic protist and retained as a photosynthetic endosymbiont. This review recounts the initial discovery and subsequent characterisation of the cryptomonad nucleomorph focusing on the key role of Peter Sitte and his protégé...

journal_title：Protoplasma

authors： McFadden GI

更新日期：2017-09-01 00:00:00