Fungal hypaphorine reduces growth and induces cytosolic calcium increase in root hairs of Eucalyptus globulus.

abstract：:Through reverse transcription-polymerase chain reaction and Northern blot analysis, we identified TBK5, a novel plant-specific kinesin-like protein (KLP) that is highly expressed in interphase tobacco BY-2 cells. TBK5 mRNA was present at a high level throughout the growth cycle, even in cells that had entered the stat...

journal_title：Protoplasma

authors： Matsui K,Collings D,Asada T

更新日期：2001-01-01 00:00:00

abstract：:Syagrus coronata is an economically important palm tree grown as an ornament, for the oil extracted from its seeds, and the wax from its leaves which has several applications in industry. Silicon biocomposites were analyzed in leaves of S. coronata. Silica bodies were found as extracellular silica masses between the h...

journal_title：Protoplasma

authors： Lins U,Barros CF,da Cunha M,Miguens FC

更新日期：2002-10-01 00:00:00

abstract：:The nature and features of organization of reserve polysaccharides in three species of the genus Pelomyxa-P. palustris, P. belevskii, and P. stagnalis-were studied using light and transmission electron microscopy. We applied the periodic acid-Schiff reaction that is a highly selective method for detecting glycogen. Th...

journal_title：Protoplasma

authors： Chistyakova L,Bezborodkina N,Berdieva M,Radaev A,Goodkov A

更新日期：2020-11-01 00:00:00

abstract：:The wild grass species Brachypodium distachyon (L.) has been proposed as a new model for temperate grasses. Among the biotechnological tools already developed for the species, an efficient induction protocol of somatic embryogenesis (SE) using immature zygotic embryos has provided the basis for genetic transformation ...

journal_title：Protoplasma

authors： Oliveira EJ,Koehler AD,Rocha DI,Vieira LM,Pinheiro MVM,de Matos EM,da Cruz ACF,da Silva TCR,Tanaka FAO,Nogueira FTS,Otoni WC

更新日期：2017-09-01 00:00:00

abstract：:Heat shock protein 90 (Hsp90) is an abundant cytosolic protein. In higher eukaryotes two isoforms of Hsp90 exist, Hsp90 alpha and Hsp90 beta. Hsp90 was purified from rat liver and after sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed a double band at about 90 kDa. The two bands were separated and id...

journal_title：Protoplasma

authors： Langer T,Fasold H

更新日期：2001-01-01 00:00:00

abstract：:Single-molecule Förster resonance energy transfer (smFRET) has emerged as a powerful tool for elucidating biological structure and mechanisms on the molecular level. Here, we focus on applications of smFRET to study interactions between DNA and enzymes such as DNA and RNA polymerases. SmFRET, used as a nanoscopic rule...

journal_title：Protoplasma

authors： Farooq S,Fijen C,Hohlbein J

更新日期：2014-03-01 00:00:00

abstract：:The detergent Brij 58 has been introduced to reverse plasma membrane (PM) vesicles from the right-side-out to the inside-out form. The aim of the present work was to investigate the effect of Brij 58 on the formation of an ATP-dependent proton gradient and on the fluidity of the lipid phase of PM vesicles. PMs of corn...

journal_title：Protoplasma

authors： Behzadipour M,Kluge M,Lüthje S

更新日期：2001-01-01 00:00:00

abstract：:The inflorescence of Hedychium coccineum Smith is thyrse, and the primary bracts are initiated in a spiral phyllotactic pattern on the sides of the inflorescence dome. Cincinnus primordia are initiated on the flank of the inflorescence apex, in the axils of primary bracts. This primordium subsequently develops a bract...

journal_title：Protoplasma

authors： Kong JJ,Xia YM,Li QJ

更新日期：2010-11-01 00:00:00

abstract：:In this study, the filamentous green alga Zygogonium ericetorum (Zygnematales, Chlorophyta), collected at its natural habitat in the high alps, was investigated by light, scanning, and transmission electron microscopy. The field samples were separated into a moist fraction when wetted by splattering water of a nearby ...

journal_title：Protoplasma

authors： Holzinger A,Tschaikner A,Remias D

更新日期：2010-07-01 00:00:00

abstract：:We present a new Chlamydomonas reinhardtii flagellar mutant in which central pair projections are missing and the central pair microtubules are twisted along the length of the flagellum. We have named this mutant tcp1 for twisted central pair. Immunoblots using an antibody that recognizes the heavy chain of sea urchin...

journal_title：Protoplasma

authors： Vucica Y,Diener DR,Rosenbaum JL,Koutoulis A

更新日期：2007-01-01 00:00:00

abstract：:The effects of arsenite treatment on generation of reactive oxygen species, induction of oxidative stress, response of antioxidative system, and synthesis of phytochelatins were investigated in two indica rice (Oryza sativa L.) cvs. Malviya-36 and Pant-12 grown in sand cultures for a period of 5-20 days. Arsenite (As(...

journal_title：Protoplasma

authors： Mishra S,Jha AB,Dubey RS

更新日期：2011-07-01 00:00:00

abstract：:The structural changes occurred in differentiating olive cotyledon cells into mesophyll cells are described. Using histological and immunocytological methods as well as microscopic observations, we showed that in the cells of mature embryo, large electron-dense proteins bodies (PBs) are surrounded by numerous oil bodi...

journal_title：Protoplasma

authors： Zienkiewicz A,Jiménez-López JC,Zienkiewicz K,de Dios Alché J,Rodríguez-García MI

更新日期：2011-10-01 00:00:00

abstract：:The terrestrial carnivorous species Utricularia dichotoma is known for a great phenotypic plasticity and unusual vegetative organs. Our investigation on 22 sources/populations revealed that after initiation of a leaf and two bladders on a stolon, a bud was formed in the proximal axil of the leaf, developing into a ros...

journal_title：Protoplasma

authors： Reut MS,Płachno BJ

更新日期：2020-03-01 00:00:00

abstract：:Pollen development and germination were adversely affected by the presence of mercury, whereas low-concentrations stimulated the whole procedure. Mercury caused morphological anomalies during the tube growth, characterized by irregularly increasing diameters and swelling tips. The main effect was the anomalous cell wa...

journal_title：Protoplasma

authors： Sawidis T,Baycu G,Cevahir-Öz G,Weryszko-Chmielewska E

更新日期：2018-05-01 00:00:00

abstract：:The cereal starch endosperm is the central part of endosperm, and it is rich in starch and protein which are the important resources for human food. The starch and protein are separately accumulated in starch granules and protein bodies. Content and configuration of starch granules and protein bodies affect the qualit...

journal_title：Protoplasma

authors： Zheng Y,Wang Z

更新日期：2015-01-01 00:00:00

abstract：:The actin-nucleating and -organizing Arp2/3 protein complex is well known to be conserved throughout the eukaryotic kingdom. For higher plants, however, only limited evidence is available for the presence of the Arp2/3 complex so far. Using heterologous antibodies against the Dictyostelium discoideum and Schizosacchar...

journal_title：Protoplasma

authors： Van Gestel K,Slegers H,Von Witsch M,Samaj J,Baluska F,Verbelen JP

更新日期：2003-09-01 00:00:00

abstract：:Nematocysts are the taxon-defining features of all cnidarians including jellyfish, sea anemones, and corals. They are highly sophisticated organelles used for the capture of prey and defense. The nematocyst capsule is produced within a giant post-Golgi vesicle, which is continuously fed by proteins from the secretory ...

journal_title：Protoplasma

authors： Ozbek S

更新日期：2011-10-01 00:00:00

abstract：:Kinesin superfamily proteins are important microtubule-based motor proteins with a kinesin motor domain that is conserved among all eukaryotic organisms. They are responsible for unidirectionally transporting various cargoes, including membranous organelles, protein complexes, and mRNAs. They also play critical roles ...

journal_title：Protoplasma

authors： Li J,Xu Y,Chong K

更新日期：2012-06-01 00:00:00

abstract：:Characean internodal cells develop alternating patterns of acid and alkaline zones along their surface in order to facilitate uptake of carbon required for photosynthesis. In this study, we used a pH-indicating membrane dye, 4-heptadecylumbiliferone, to study the kinetics of alkaline band formation and decomposition. ...

journal_title：Protoplasma

authors： Eremin A,Bulychev AA,Kluge C,Harbinson J,Foissner I

更新日期：2019-11-01 00:00:00

abstract：:The effect of cytochalasin B (CB; 25 micrograms ml-1 in 1% dimethylsulfoxide, DMSO) upon the structural polarity of statocytes in cress roots is demonstrated. If normal, vertically grown roots are incubated in CB, the structural polarity of the statocytes is altered according to the developmental stage of the root. St...

journal_title：Protoplasma

authors： Hensel W

更新日期：1985-01-01 00:00:00

abstract：:The small GTPase CDC42 is ubiquitously expressed in eukaryotes, where it participates in the regulation of the cytoskeleton and a wide range of cellular processes, including cytokinesis, gene expression, cell cycle progression, apoptosis, and tumorigenesis. As very little is known on the molecular level about mycorrhi...

journal_title：Protoplasma

authors： Menotta M,Amicucci A,Basili G,Rivero F,Polidori E,Sisti D,Stocchi V

更新日期：2007-01-01 00:00:00

abstract：:Telocytes (TCs), a novel type of interstitial cells, are involved in tissue homeostasis maintenance. This study aimed to investigate TC presence in the interstitium of mouse testis. Additionally, inactivation of the G-coupled membrane estrogen receptor (GPER) in the testis was performed to obtain insight into TC funct...

journal_title：Protoplasma

authors： Pawlicki P,Hejmej A,Milon A,Lustofin K,Płachno BJ,Tworzydlo W,Gorowska-Wojtowicz E,Pawlicka B,Kotula-Balak M,Bilinska B

更新日期：2019-03-01 00:00:00

abstract：:The mature apomictic embryo sac of Chondrilla juncea is highly vacuolated and demonstrates a polarization similar to that of the amphimictic gametophyte. The microtubule cytoskeleton of this embryo sac is uncharacteristic and relatively weak. The microtubules are positioned along cell walls and resemble cortical micro...

journal_title：Protoplasma

authors： Kościńska-Pajak M,Bednara J

更新日期：2006-05-01 00:00:00

abstract：:The osmotic potential and effects of plasmolysis were investigated in two different Klebsormidium strains from alpine habitats by incubation in 300-2,000 (3,000) mM sorbitol. Several members of this genus were previously found to tolerate desiccation in the vegetative state yet information was lacking on the osmotic p...

journal_title：Protoplasma

authors： Kaplan F,Lewis LA,Wastian J,Holzinger A

更新日期：2012-07-01 00:00:00

abstract：:Tradescantia virginiana leaf epidermal cells were plasmolysed by sequential treatment with 0.8 M and 0.3 M sucrose. Plasmolysis revealed adhesion of the plasma membrane to the cell wall at sites coinciding with cytoskeletal arrays involved in the polarisation of cells undergoing asymmetric divisions--cortical actin pa...

journal_title：Protoplasma

authors： Cleary AL

更新日期：2001-01-01 00:00:00

abstract：:In order to gain more insight into the involvement of mitochondrial complex III in the Cd-induced stress, we studied the effect of complex III inhibitors, antimycin A (AA), and myxothiazol (MYXO), on the Cd-induced ROS and NO generation in the barley root tip. Short-term exposure of barley roots to either MYXO or AA p...

journal_title：Protoplasma

authors： Zelinová V,Demecsová L,Tamás L

更新日期：2019-09-01 00:00:00

abstract：:Radial oxygen loss is a physical phenomenon that occurs naturally in aquatic plants. Typha domingensis was chosen as a model plant because it possesses basic morphological characteristics, such as a stem (rhizome) that produces leaves and adventitious roots, which are present in many aquatic plants. This study aimed t...

journal_title：Protoplasma

authors： Duarte VP,Pereira MP,Corrêa FF,de Castro EM,Pereira FJ

更新日期：2021-01-06 00:00:00

abstract：:We discuss models for production of tubulin flux in kinetochore microtubules. Current models concentrate solely on microtubules and their associated motors and enzymes. For example, in some models the driving force for flux is enzymes at the poles and the kinetochores; in others the driving force is motor molecules th...

journal_title：Protoplasma

authors： Forer A,Pickett-Heaps JD,Spurck T

更新日期：2008-01-01 00:00:00

abstract：:A convenient experimental system was established to test how cells derived from higher-plant internal tissues respond to mechanical stimulation. Short-term culture of tobacco ovules in vitro led to the generation of bar-shaped cells from the parenchyma tissue of the ovule funicle. These cells are still connected to th...

journal_title：Protoplasma

authors： Qu LH,Sun MX

更新日期：2008-01-01 00:00:00

abstract：:Frost resistance of sprouting Picea abies shoots is insufficient for survival of naturally occurring late frosts. The cellular changes during sprouting appeared to be responsible for frost damage as frost events that damaged sprouting shoots did not damage older needles and stems. Whilst resting buds showed initial fr...

journal_title：Protoplasma

authors： Neuner G,Beikircher B

更新日期：2010-07-01 00:00:00