Abstract：
:We present a new Chlamydomonas reinhardtii flagellar mutant in which central pair projections are missing and the central pair microtubules are twisted along the length of the flagellum. We have named this mutant tcp1 for twisted central pair. Immunoblots using an antibody that recognizes the heavy chain of sea urchin kinesin reveal that a 70 kDa protein present in wild-type and pf18 (central pairless) axonemes is absent in tcp1, suggesting the presence of an uncharacterized kinesin associated with the central pair apparatus. We demonstrate that the kinesin-like protein Klp1 is not attached to central pair microtubules in tcp1, but rather is located in, or is part of, a region we have termed the internal axonemal matrix. It is proposed that this matrix acts as a scaffold for axonemal proteins that may also be associated with the central pair apparatus.
journal_name
Protoplasmajournal_title
Protoplasmaauthors
Vucica Y,Diener DR,Rosenbaum JL,Koutoulis Adoi
10.1007/s00709-007-0273-8subject
Has Abstractpub_date
2007-01-01 00:00:00pages
121-30issue
1-2eissn
0033-183Xissn
1615-6102journal_volume
232pub_type
杂志文章相关文献
PROTOPLASMA文献大全abstract：:Ripening which leads to fruit senescence is an inimitable process characterized by vivid changes in color, texture, flavor, and aroma of the fleshy fruits. Our understanding of the mechanisms underlying the regulation of fruit ripening and senescence is far from complete. Molecular and biochemical studies on tomato (S...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-015-0836-z
更新日期：2016-03-01 00:00:00
abstract：:The cryptomonad nucleomorph is a vestigial nucleus of a eukaryotic red alga engulfed by a phagotrophic protist and retained as a photosynthetic endosymbiont. This review recounts the initial discovery and subsequent characterisation of the cryptomonad nucleomorph focusing on the key role of Peter Sitte and his protégé...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-017-1153-5
更新日期：2017-09-01 00:00:00
abstract：:Superoxide dismutase (SOD) activities of the oomycete Phytophthora cinnamomi were examined. Five polypeptides with manganese superoxide dismutase (MnSOD) activity were found in mycelium growing in liquid culture with relative molecular weights ranging from approximately 25 to 100 kDa. Comparison with characterized avo...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-012-0464-9
更新日期：2013-06-01 00:00:00
abstract：:Developing flowers are important sinks in Arabidopsis thaliana. Their energy demand is covered by assimilates which are synthesized in source leaves and transported via the vasculature. Assimilates are unloaded either symplastically through plasmodesmata or apoplastically by specific transport proteins. Here we studie...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-010-0223-8
更新日期：2011-01-01 00:00:00
abstract：:Characean internodal cells develop alternating patterns of acid and alkaline zones along their surface in order to facilitate uptake of carbon required for photosynthesis. In this study, we used a pH-indicating membrane dye, 4-heptadecylumbiliferone, to study the kinetics of alkaline band formation and decomposition. ...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-019-01392-0
更新日期：2019-11-01 00:00:00
abstract：:Radial oxygen loss is a physical phenomenon that occurs naturally in aquatic plants. Typha domingensis was chosen as a model plant because it possesses basic morphological characteristics, such as a stem (rhizome) that produces leaves and adventitious roots, which are present in many aquatic plants. This study aimed t...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-020-01597-8
更新日期：2021-01-06 00:00:00
abstract：:Isolated microspores of B. napus in culture change their developmental pathway from gametophytic to sporophytic and form embryo-like structures (ELS) upon prolonged heat shock treatment (5 days at 32 °C). ELS express polarity during the initial days of endosporic development. In this study, we focussed on the analysis...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-013-0530-y
更新日期：2014-01-01 00:00:00
abstract：:The actin cytoskeleton is involved in the positioning of statoliths in tip growing Chara rhizoids. The balance between the acropetally acting gravity force and the basipetally acting net outcome of cytoskeletal force results in the dynamically stable position of the statoliths 10-30 micrometers above the cell tip. A c...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/BF01404042
更新日期：1993-01-01 00:00:00
abstract：:In the present investigation, we have used adenosine triphosphatase (ATPase) activity as biochemical test of toxic action of lindane that was explained by lipid peroxidation model. Study was also undertaken to ascertain the potential protective role of alpha-lipoic acid (ALA) and vitamin E on the same parameters. High...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-010-0121-0
更新日期：2010-06-01 00:00:00
abstract：:The wild grass species Brachypodium distachyon (L.) has been proposed as a new model for temperate grasses. Among the biotechnological tools already developed for the species, an efficient induction protocol of somatic embryogenesis (SE) using immature zygotic embryos has provided the basis for genetic transformation ...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-017-1089-9
更新日期：2017-09-01 00:00:00
abstract：:Lemna species are reported to accumulate a variety of metals from contaminated/polluted sites. Cadmium is a nonessential element for plant metabolism. In this work, we aimed to investigate physiological responses to low doses of cadmium (up to 100 microM). From exposure to the lowest Cd concentration (1 microM) to the...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-009-0091-2
更新日期：2010-04-01 00:00:00
abstract：:The dinoflagellate Crypthecodinium cohnii Biecheler propagates by both binary and multiple fission. By a newly developed mutagenesis protocol based on using ethyl methanesulfonate and a cell size screening method, a cell cycle mutant, mf2, was isolated with giant cells which predominantly divide by multiple fission. T...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/BF02680134
更新日期：2001-01-01 00:00:00
abstract：:The aim of the study was to elucidate the effect of cadmium ions on the arrangement of the actin and tubulin cytoskeleton, as well as the relationships between cytoskeletal changes and growth processes in the green filamentous alga Spirogyra decimina. Batch cultures of algae were carried out under defined conditions i...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-005-0123-5
更新日期：2005-12-01 00:00:00
abstract：:A comparative analysis of fluorochrome-binding pattern in nine taxa of Abelmoschus had shown that the type, amount and distribution pattern of heterochromatin were characteristic for each taxa. The fluorescent chromosome-binding sites obtained by chromomycin A3 (CMA) and 4',6-diamidino-2-phenylindole (DAPI) staining i...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-014-0712-2
更新日期：2015-03-01 00:00:00
abstract：:In order to gain more insight into the involvement of mitochondrial complex III in the Cd-induced stress, we studied the effect of complex III inhibitors, antimycin A (AA), and myxothiazol (MYXO), on the Cd-induced ROS and NO generation in the barley root tip. Short-term exposure of barley roots to either MYXO or AA p...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-019-01389-9
更新日期：2019-09-01 00:00:00
abstract：:Podophyllum species (Podophyllum hexandrum Royle and Podophyllum peltatum) are a major source of deriving anticancer drugs from their major chemical constituent, podophyllotoxin. However, information lacks on regulatory components of podophyllotoxin biosynthesis; therefore, different classes of transcription factors w...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-015-0938-7
更新日期：2017-01-01 00:00:00
abstract：:Many studies have been carried out to investigate the histological structure of the trachea in many species of birds. However, the cellular organization of the trachea in the mallard duck is still unclear. This study was performed on 12 sexually mature male Mallard duck to demonstrate the cellular organization of the ...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-019-01444-5
更新日期：2020-03-01 00:00:00
abstract：:Salinity is the major environmental constraint that affects legume productivity by inducing oxidative stress. Individually, both silicon (Si) nutrition and mycorrhization have been reported to alleviate salt stress. However, the mechanisms adopted by both in mediating stress responses are poorly understood. Thus, pot ...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-015-0892-4
更新日期：2016-09-01 00:00:00
abstract：:Pollen developmental pathway in plants involving synchronized transferal of cellular divisions from meiosis (microsporogenesis) to mitosis (pollen mitosis I/II) eventually offers a unique "meiosis-mitosis shift" at pollen mitosis I. Since the cell type (haploid microspore) and fate of pollen mitosis I differ from typi...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-015-0925-z
更新日期：2017-01-01 00:00:00
abstract：:The purpose of the main research was to investigate the effects of methyl jasmonate (MeJA) (0.05, 0.25, 0.5, and 2.5 mM) on the pollen germination and tube elongation of Pinus nigra. Total pollen germination rate increased after MeJA treatments while the most enhancement was observed at 0.05-mM MeJA. No germination wa...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-020-01539-4
更新日期：2020-11-01 00:00:00
abstract：:By a detailed ontogenetic study of Polemonium caeruleum pollen, tracing each stage of development at high TEM resolution, we aim to understand the establishment of the pollen wall and to unravel the mechanisms underlying sporoderm development. The main steps of exine ontogeny in Polemonium caeruleum, observed in the m...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-017-1121-0
更新日期：2018-01-01 00:00:00
abstract：:Abiotic stresses, such as drought and high salinity, are major factors that limit plant growth and productivity. Late embryogenesis abundant (LEA) proteins are members of a diverse, multigene family closely associated with tolerance to abiotic stresses in numerous organisms. We examined the function of SmLEA2, previou...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-016-0981-z
更新日期：2017-03-01 00:00:00
abstract：:Here, we describe the development of chloroplasts and the buildup of the thylakoid membranes in growing Arabidopsis leaves. Organelles were analyzed from three distinct positions, namely, at the tip, the upper leaf margin, and the base from leaves 1, 3, 5, and 7 of 14-day-old plants. Clear developmental gradients are ...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-016-1057-9
更新日期：2017-09-01 00:00:00
abstract：:Cell polarity, the asymmetric organization of cellular components along one or multiple axes, is present in most cells. From budding yeast cell polarization induced by pheromone signaling, oocyte polarization at fertilization to polarized epithelia and neuronal cells in multicellular organisms, similar mechanisms are ...
journal_title：Protoplasma
pub_type： 杂志文章,评审
doi：10.1007/s00709-017-1075-2
更新日期：2017-05-01 00:00:00
abstract：:Endoplasmic reticulum (ER) immunolabeling in developing stomatal complexes and in the intervening cells of the stomatal rows (ICSRs) of Zea mays revealed that the cortical-ER forms distinct aggregations lining locally expanding wall regions. The polarized subsidiary cell mother cells (SMCs), displayed a cortical-ER-pa...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-010-0180-2
更新日期：2011-04-01 00:00:00
abstract：:The present study reports the Agrobacterium rhizogenes-mediated hairy root induction in of an ethno-medicinally significant herb-Boerhaavia diffusa L., for elucidating the underlying competence regarding its biosynthetic (i.e. boeravinone B and eupalitin) and bioactivity (antibacterial, antioxidant and anti-inflammato...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-015-0875-5
更新日期：2016-07-01 00:00:00
abstract：:In this study, we have shown the applicability of chloramphenicol acetyltransferase as a new and convenient selectable marker for stable nuclear transformation as well as potential chloroplast transformation of Cyanidioschyzon merolae-a new model organism, which offers unique opportunities for studding the mitochondri...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-015-0936-9
更新日期：2017-01-01 00:00:00
abstract：:Ascochyta pisi is a necrotrophic pathogenic fungus, which mainly survives between seasons through infected seeds. Defence responses of pea embryo axes to A. pisi were investigated in the heterotrophic phase of seed germination and during the transition from the heterotrophic to the autotrophic phase. Germinated pea se...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-012-0374-x
更新日期：2013-02-01 00:00:00
abstract：:Interactive effects of two heavy metal pollutants Cd and Pb in the growth medium were examined on their uptake, production of reactive oxygen species (ROS), induction of oxidative stress and antioxidative defence responses in Indica rice (Oryza sativa L.) seedlings. When rice seedlings in sand culture were exposed to ...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-014-0614-3
更新日期：2014-09-01 00:00:00
abstract：:HLA-B associated transcript 1 (BAT1) protein, also named as spliceosome RNA helicase UAP56, is a member of the DExD/H-box family of helicases. However, regulation under stress, biochemical properties, and functions of plant homologue of BAT1 are poorly understood. Here, we report the purification and detailed biochemi...
journal_title：Protoplasma
pub_type： 杂志文章
doi：10.1007/s00709-015-0791-8
更新日期：2015-11-01 00:00:00
