Abstract:
:Isolated microspores of B. napus in culture change their developmental pathway from gametophytic to sporophytic and form embryo-like structures (ELS) upon prolonged heat shock treatment (5 days at 32 °C). ELS express polarity during the initial days of endosporic development. In this study, we focussed on the analysis of polarity development of ELS without suspensor. Fluorescence microscopy and 3-D confocal laser scanning microscopy (CLSM) without tissue interfering enabled us to get a good insight in the distribution of nuclei, mitochondria and endoplasmic reticulum (ER), the architecture of microtubular (MT) cytoskeleton and the places of 5-bromo-2'-deoxy-uridine (BrdU) incorporation in successive stages of microspore embryogenesis. Scanning electron microscopy (SEM) analysis revealed, for the first time, the appearance of a fibrillar extracellular matrix-like structure (ECM-like structure) in androgenic embryos without suspensor. Two types of endosporic development were distinguished based upon the initial location of the microspore nucleus. The polarity of dividing and growing cells was recognized by the differential distributions of organelles, by the organization of the MT cytoskeleton and by the visualization of DNA synthesis in the cell cycle. The directional location of nuclei, ER, mitochondria and starch grains in relation to the MTs configurations were early polarity indicators. Both exine rupture and ECM-like structure on the outer surfaces of ELS are supposed to stabilize ELS's morphological polarity. As the role of cell polarity during early endosporic microspore embryogenesis in apical-basal cell fate determination remains unclear, microspore culture system provides a powerful in vitro tool for studying the developmental processes that take place during the earliest stages of plant embryogenesis.
journal_name
Protoplasmajournal_title
Protoplasmaauthors
Dubas E,Custers J,Kieft H,Wędzony M,van Lammeren AAdoi
10.1007/s00709-013-0530-ysubject
Has Abstractpub_date
2014-01-01 00:00:00pages
103-13issue
1eissn
0033-183Xissn
1615-6102journal_volume
251pub_type
杂志文章相关文献
PROTOPLASMA文献大全abstract::Chlamydomonas acidophila LAFIC-004 is an acidophilic strain of green microalgae isolated from coal mining drainage. In the present work, this strain was cultivated in acidic medium (pH 3.6) under phototrophic, mixotrophic, and heterotrophic regimes to determine the best condition for growth and lipid production, simul...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-016-1030-7
更新日期:2017-05-01 00:00:00
abstract::Hyperhydricity is a physiological disorder associated with oxidative stress. Reactive oxygen species (ROS) generation in plants is initiated by various enzymatic sources, including plasma membrane-localized nicotinamide adenine dinucleotide phosphate (NADPH) oxidases, cell wall-bound peroxidase (POD), and apoplastic p...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-016-0957-z
更新日期:2017-01-01 00:00:00
abstract::In this study, we surveyed the bacteriome-associated microbiota of the corn leafhopper Dalbulus maidis by means of histological, ultrastructural, and molecular analyses. Amplification and sequencing of 16S rDNA genes revealed that the endosymbiont "Candidatus Sulcia muelleri" (Phylum Bacteroidetes) resides in bacterio...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-016-1033-4
更新日期:2017-05-01 00:00:00
abstract::The extent to which the actomyosin motor responsible for cytoplasmic streaming contributes to the translational diffusion of water in Elodea canadensis cells was studied by a nuclear magnetic resonance (NMR) spin-echo technique. The relative contribution of the actomyosin motor was determined from the corresponding ap...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-004-0067-1
更新日期:2004-12-01 00:00:00
abstract::In the grass snake (Natrix natrix), the newly developed somites form vesicles that are located on both sides of the neural tube. The walls of the vesicles are composed of tightly connected epithelial cells surrounding the cavity (the somitocoel). Also, in the newly formed somites, the Pax3 protein can be observed in t...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-016-1040-5
更新日期:2017-07-01 00:00:00
abstract::Podophyllum species (Podophyllum hexandrum Royle and Podophyllum peltatum) are a major source of deriving anticancer drugs from their major chemical constituent, podophyllotoxin. However, information lacks on regulatory components of podophyllotoxin biosynthesis; therefore, different classes of transcription factors w...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-015-0938-7
更新日期:2017-01-01 00:00:00
abstract::Despite great efforts in tissue engineering of the ureter, urinary bladder, and urethra, further research is needed in order to improve the patient's quality of life and minimize the economic burden of different lower urinary tract disorders. The nanostructured titanium dioxide (TiO2) scaffolds have a wide range of cl...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-015-0896-0
更新日期:2016-11-01 00:00:00
abstract::Reactive carbonyl species methylglyoxal (MG) has always been regarded as a cytotoxic metabolite, but now is emerging to function as signal molecule in plants. However, whether MG can induce salt tolerance is elusive. In this study, treatment of wheat seeds with NaCl reduced seed germination, plant height, root length,...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-017-1094-z
更新日期:2017-09-01 00:00:00
abstract::Many studies have been carried out to investigate the histological structure of the trachea in many species of birds. However, the cellular organization of the trachea in the mallard duck is still unclear. This study was performed on 12 sexually mature male Mallard duck to demonstrate the cellular organization of the ...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-019-01444-5
更新日期:2020-03-01 00:00:00
abstract::Fragile sites (FSs) in plants have been described for species like Lolium and other grasses. Whereas in humans FSs were shown to be involved in genome instabilities; the consequences of FSs expression in plants are not known yet. To evaluate whether FSs cause karyotype instabilities, we assessed the frequency of micro...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-016-0942-6
更新日期:2017-01-01 00:00:00
abstract::Through reverse transcription-polymerase chain reaction and Northern blot analysis, we identified TBK5, a novel plant-specific kinesin-like protein (KLP) that is highly expressed in interphase tobacco BY-2 cells. TBK5 mRNA was present at a high level throughout the growth cycle, even in cells that had entered the stat...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/BF01280307
更新日期:2001-01-01 00:00:00
abstract::In this paper, two transgenic wheat lines, PC27 and PC51, containing the maize PEPC gene and its wild-type (WT) were used as experimental material to study the effects of high temperature on their photosynthetic physiological characteristics and metabolome. The results showed that transgenic wheat lines had higher pho...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-016-1010-y
更新日期:2017-03-01 00:00:00
abstract::Radial oxygen loss is a physical phenomenon that occurs naturally in aquatic plants. Typha domingensis was chosen as a model plant because it possesses basic morphological characteristics, such as a stem (rhizome) that produces leaves and adventitious roots, which are present in many aquatic plants. This study aimed t...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-020-01597-8
更新日期:2021-01-06 00:00:00
abstract::Fluorescence in situ hybridization revealed the presence of ribosomal RNA genes in paracentromeric regions of all A chromosomes and in the distal half of B chromosomes in embryonic cells from Moroccan specimens of the grasshopper Eyprepocnemis plorans. The expression of these genes was monitored by the presence of nuc...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/BF01283399
更新日期:2001-01-01 00:00:00
abstract::Sugarcane shows reduced crop stand under relatively suboptimal conditions; the main reason for this is its sensitivity to ionic stress in the soil solution. This research was performed to explore some physiological and developmental changes in the immature sugarcane buds submitted to salt stress and possible role of g...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-015-0841-2
更新日期:2016-05-01 00:00:00
abstract::The histochemical studies that search for osmophores differ in the protocol they follow for fixation and discoloration of the samples, and also in the type and number of stains utilized. Despite these differences, all the studies have one point in common: the use of fresh material either collected directly in the fiel...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-019-01398-8
更新日期:2019-11-01 00:00:00
abstract::In the Cape Fynbos of South Africa, Psoralea pinnata (L.) plants occur naturally in both wetland and well-drained soils and yet effectively fix N2 under the two contrasting conditions. In this study, nodule structure and functioning in P. pinnata plants from the two habitats were evaluated using light and transmission...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-015-0922-2
更新日期:2017-01-01 00:00:00
abstract::Vacuoles are ubiquitous organelles in the fungal and plant kingdoms. They serve a variety of functions and are important for cell homeostasis. A constant turnover of proteins and membranes makes vacuoles dynamic organelles. Various transport pathways share the vacuole as their joint destination. The trafficking pathwa...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/BF01415696
更新日期:1999-01-01 00:00:00
abstract::The present study focuses on the responses of floating laminae of the Mn-tolerant hydrophyte Trapa natans L. to 1 mM Mn and their ability to accumulate the metal. Studies were carried out first on young floating laminae belonging to the second verticil of 30-day-old plants which originated from fruits that had been ma...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-007-0242-2
更新日期:2007-01-01 00:00:00
abstract::Endoplasmic reticulum (ER) is a major site for secretory protein folding and lipid synthesis. Since ER cannot be synthesized de novo, it must be inherited during the cell cycle. Studying ER inheritance can however be difficult because the ER of typical plant and animal cells is morphologically complex. Therefore, our ...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-011-0359-1
更新日期:2012-10-01 00:00:00
abstract::Brachypodium distachyon has emerged as a model plant for the improvement of grain crops such as wheat, barley and oats and for understanding basic biological processes to facilitate the development of grasses as superior energy crops. Brachypodium is also the first species of the grass subfamily Pooideae with a sequen...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-014-0689-x
更新日期:2015-03-01 00:00:00
abstract::Short-time temperature effects (34-40 °C) on microtubule (MT) organization and on cell structure of young epidermal leaf cells of the seagrass Cymodocea nodosa were investigated under laboratory conditions using transmission electron microscopy (TEM) and tubulin immunofluorescence. The interphase MT network was affect...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-015-0809-2
更新日期:2016-03-01 00:00:00
abstract::The presence of specific protein types in claws and beaks of turtles is poorly known. The present immunological study describes the localization of some of the main corneous beta proteins (CBPs) coded in the epidermal differentiation complex of turtles. Three antibodies here utilized revealed that glycine-, cysteine-,...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-020-01494-0
更新日期:2020-07-01 00:00:00
abstract::Drought tolerance is a complex trait that is governed by multiple genes. The study presents differential transcriptome analysis between drought-tolerant (Triticum aestivum Cv. C306) and drought-sensitive (Triticum aestivum Cv. WL711) genotypes, using Affymetrix GeneChip® Wheat Genome Array. Both genotypes exhibited di...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-018-1237-x
更新日期:2018-09-01 00:00:00
abstract::In the present investigation, we have used adenosine triphosphatase (ATPase) activity as biochemical test of toxic action of lindane that was explained by lipid peroxidation model. Study was also undertaken to ascertain the potential protective role of alpha-lipoic acid (ALA) and vitamin E on the same parameters. High...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-010-0121-0
更新日期:2010-06-01 00:00:00
abstract::Programmed cell death occurs in plants during several developmental processes and during the expression of resistance to pathogen attack (i.e., the hypersensitive response). An unsolved question of plant programmed cell death is whether a unique signaling pathway or different, possibly convergent pathways exist. This ...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-004-0053-7
更新日期:2004-10-01 00:00:00
abstract::This article deals with the distribution of callose and of the homogalacturonan (HG) epitopes recognized by LM20, JIM5, and 2F4 antibodies in cell walls of differentiating and functioning stomatal complexes of the monocotyledon Zea mays and the dicotyledon Vigna sinensis. The findings revealed that, during stomatal de...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-019-01425-8
更新日期:2020-01-01 00:00:00
abstract::Pongamia pinnata L. is a multipurpose versatile legume that is well known as a prospective feedstock biodiesel species. However, to date, there has been little genomic research aimed at the exploitation of the biotechnological potential of this species. Genetic characterization of any plant is a challenging task when ...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-013-0545-4
更新日期:2014-05-01 00:00:00
abstract::Indirect immunofluorescence and confocal microscopy were used to study the nucleation and organization of microtubules during meiosis in two species of leafy liverworts, Cephalozia macrostachya and Telaranea longifolia. This is the first such study of sporogenesis in the largest group of liverworts important as living...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-009-0073-4
更新日期:2009-10-01 00:00:00
abstract::Temperatures above the optimum are sensed as heat stress (HS) by all living organisms and represent one of the major environmental challenges for plants. Plants can cope with HS by activating specific defense mechanisms to minimize damage and ensure cellular functionality. One of the most common effects of HS is the o...
journal_title:Protoplasma
pub_type: 杂志文章
doi:10.1007/s00709-017-1200-2
更新日期:2018-07-01 00:00:00