Involvement of microtubules in rhizoid differentiation of Spirogyra species.

abstract：:Features of programmed cell death (PCD) and dynamic changes of starch accumulation in developing pericarp cells of wheat (Triticum aestivum L.) were observed and analyzed by periodic acid-Schiff/toluidine blue O double staining, fluorescence staining, terminal deoxynucleotidyl transferase-mediated fluorescein deoxyuri...

journal_title：Protoplasma

authors： Zhou Z,Wang L,Li J,Song X,Yang C

更新日期：2009-07-01 00:00:00

abstract：:Previous complementation of a chitin synthase class Vb null mutant (Colletotrichum graminicola chsA) indicated that the encoded protein is responsible for approximately 30% of the conidial chitin, is essential for conidial wall strength in media with high water potential, and contributes to strength of hyphal tips. We...

journal_title：Protoplasma

authors： Amnuaykanjanasin A,Epstein L

更新日期：2006-05-01 00:00:00

abstract：:Vacuoles are ubiquitous organelles in the fungal and plant kingdoms. They serve a variety of functions and are important for cell homeostasis. A constant turnover of proteins and membranes makes vacuoles dynamic organelles. Various transport pathways share the vacuole as their joint destination. The trafficking pathwa...

journal_title：Protoplasma

authors： Götte M,Lazar T

更新日期：1999-01-01 00:00:00

abstract：:Formation of intra-molecular disulfides and concomitant oxidative protein folding is essential for stability and catalytic function of many soluble and membrane-bound proteins in the endomembrane system, the mitochondrial inter-membrane space and the thylakoid lumen. Disulfide generation from free cysteines in nascent...

journal_title：Protoplasma

authors： Aller I,Meyer AJ

更新日期：2013-08-01 00:00:00

abstract：:We used qualitative and quantitative fluorescence microscopy of the fluorescein isothiocyanate-conjugated lectins Concanavalin A, phytohaemagglutinin-erythroagglutinin, pokeweed mitogen, and peanut agglutinin to examine sugar composition on the cell surface and cell-associated mucilage (where present) in a number of c...

journal_title：Protoplasma

authors： Tien CJ,Sigee DC,White KN

更新日期：2005-10-01 00:00:00

abstract：:We present a new Chlamydomonas reinhardtii flagellar mutant in which central pair projections are missing and the central pair microtubules are twisted along the length of the flagellum. We have named this mutant tcp1 for twisted central pair. Immunoblots using an antibody that recognizes the heavy chain of sea urchin...

journal_title：Protoplasma

authors： Vucica Y,Diener DR,Rosenbaum JL,Koutoulis A

更新日期：2007-01-01 00:00:00

abstract：:Mechanisms of transport of secretory products across the Golgi apparatus (GA) as well as of scale formation in prymnesiophytes have remained controversial. We have used a quantitative morphological approach to study formation and transport of scales across the GA in haploid cells of Pleurochrysis sp. The GA of these c...

journal_title：Protoplasma

authors： Hawkins EK,Lee JJ

更新日期：2001-01-01 00:00:00

abstract：:Characean internodal cells develop alternating patterns of acid and alkaline zones along their surface in order to facilitate uptake of carbon required for photosynthesis. In this study, we used a pH-indicating membrane dye, 4-heptadecylumbiliferone, to study the kinetics of alkaline band formation and decomposition. ...

journal_title：Protoplasma

authors： Eremin A,Bulychev AA,Kluge C,Harbinson J,Foissner I

更新日期：2019-11-01 00:00:00

abstract：:Despite great efforts in tissue engineering of the ureter, urinary bladder, and urethra, further research is needed in order to improve the patient's quality of life and minimize the economic burden of different lower urinary tract disorders. The nanostructured titanium dioxide (TiO2) scaffolds have a wide range of cl...

journal_title：Protoplasma

authors： Imani R,Pazoki M,Zupančič D,Kreft ME,Kralj-Iglič V,Veranič P,Iglič A

更新日期：2016-11-01 00:00:00

abstract：:Mastigonemes, tripartite tubular hairs on the anterior flagellum of Phytophthora zoospores, are instrumental for disease dissemination to new host plants. A previous study showed that PnMas2 was part of the tubular shaft of Phytophthora parasitica mastigonemes. In the current study, genes encoding two related proteins...

journal_title：Protoplasma

authors： Hee WY,Blackman LM,Hardham AR

更新日期：2019-03-01 00:00:00

abstract：:We used a noninvasive microprobe technique to record in substomatal cavities of barley leaves the apoplastic pH response to different stress situations. When K+ (or Na+) activity at the roots of intact plants was increased from 1 to 50 mM, the leaf apoplastic pH increased by 0.4 to 0.6 units within 8 to 12 min when st...

journal_title：Protoplasma

authors： Felle HH,Herrmann A,Hückelhoven R,Kogel KH

更新日期：2005-12-01 00:00:00

abstract：:A survey is presented of the architecture of secondary wall ingrowths in transfer cells from various taxa based on scanning electron microscopy. Wall ingrowths are a distinguishing feature of transfer cells and serve to amplify the plasma membrane surface area available for solute transport. Morphologically, two categ...

journal_title：Protoplasma

authors： Talbot MJ,Offler CE,McCurdy DW

更新日期：2002-05-01 00:00:00

abstract：:The osmotic potential and effects of plasmolysis were investigated in two different Klebsormidium strains from alpine habitats by incubation in 300-2,000 (3,000) mM sorbitol. Several members of this genus were previously found to tolerate desiccation in the vegetative state yet information was lacking on the osmotic p...

journal_title：Protoplasma

authors： Kaplan F,Lewis LA,Wastian J,Holzinger A

更新日期：2012-07-01 00:00:00

abstract：:Ripening which leads to fruit senescence is an inimitable process characterized by vivid changes in color, texture, flavor, and aroma of the fleshy fruits. Our understanding of the mechanisms underlying the regulation of fruit ripening and senescence is far from complete. Molecular and biochemical studies on tomato (S...

journal_title：Protoplasma

authors： Kumar V,Irfan M,Ghosh S,Chakraborty N,Chakraborty S,Datta A

更新日期：2016-03-01 00:00:00

abstract：:Through reverse transcription-polymerase chain reaction and Northern blot analysis, we identified TBK5, a novel plant-specific kinesin-like protein (KLP) that is highly expressed in interphase tobacco BY-2 cells. TBK5 mRNA was present at a high level throughout the growth cycle, even in cells that had entered the stat...

journal_title：Protoplasma

authors： Matsui K,Collings D,Asada T

更新日期：2001-01-01 00:00:00

abstract：:Heat shock protein 90 (Hsp90) is an abundant cytosolic protein. In higher eukaryotes two isoforms of Hsp90 exist, Hsp90 alpha and Hsp90 beta. Hsp90 was purified from rat liver and after sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed a double band at about 90 kDa. The two bands were separated and id...

journal_title：Protoplasma

authors： Langer T,Fasold H

更新日期：2001-01-01 00:00:00

abstract：:Iron (Fe) toxicity is one of the major abiotic stresses which limits the yield of lowland rice. This study aims to investigate the physiological, biochemical, and molecular aspects of two contrasting aromatic Joha rice, viz., Keteki and Kola Joha of Assam. Oxidative damage caused due to Fe2+ toxicity was quantitativel...

journal_title：Protoplasma

authors： Regon P,Dey S,Chowardhara B,Saha B,Kar S,Tanti B,Panda SK

更新日期：2020-10-17 00:00:00

abstract：:Pollen ontogeny in Pancratium maritimum L. was studied from the sporogenous cell to mature pollen grain stages using transmission electron, scanning electron, and light microscopy to determine whether the pollen development in P. maritimum follows the basic scheme in angiosperms or not. In the course of microsporogene...

journal_title：Protoplasma

authors： Tütüncü Konyar S

更新日期：2017-03-01 00:00:00

abstract：:Root hairs are tubular cells resulting from a tip-localized growth in which calcium ions play a key role. Hypaphorine, an indole alkaloid secreted by the fungus Pisolithus microcarpus during the formation of ectomycorrhizae with the host plant Eucalyptus globulus, inhibits root hair tip growth. Hypaphorine-induced inh...

journal_title：Protoplasma

authors： Dauphin A,Gérard J,Lapeyrie F,Legué V

更新日期：2007-01-01 00:00:00

abstract：:Treatment of 14-day-old maize cultivars (Hybrid351 and Giza2) with 250 mM NaCl significantly reduced shoot fresh and dry weights and protein content during the subsequent 12 days. The magnitude of reduction was more pronounced in Giza than Hybrid. Both cultivars contained converging levels of protein for the enzymes p...

journal_title：Protoplasma

authors： Alla MM,Hassan NM

更新日期：2012-10-01 00:00:00

abstract：:The cereal starch endosperm is the central part of endosperm, and it is rich in starch and protein which are the important resources for human food. The starch and protein are separately accumulated in starch granules and protein bodies. Content and configuration of starch granules and protein bodies affect the qualit...

journal_title：Protoplasma

authors： Zheng Y,Wang Z

更新日期：2015-01-01 00:00:00

abstract：:While there is a number of studies on the effects of Rho GTPases on the actin-based cytoskeleton in higher eukaryotes, studies in protozoans are rather limited. The problem seems to be intriguing since the structure of protozoan cytoskeletons is distinct from most vertebrate cells. By blocking endogenous Rho family pr...

journal_title：Protoplasma

authors： Kłopocka W,Redowicz MJ

更新日期：2003-03-01 00:00:00

abstract：:Programmed cell death occurs in plants during several developmental processes and during the expression of resistance to pathogen attack (i.e., the hypersensitive response). An unsolved question of plant programmed cell death is whether a unique signaling pathway or different, possibly convergent pathways exist. This ...

journal_title：Protoplasma

authors： Malerba M,Cerana R,Crosti P

更新日期：2004-10-01 00:00:00

abstract：:Drought tolerance is a complex trait that is governed by multiple genes. The study presents differential transcriptome analysis between drought-tolerant (Triticum aestivum Cv. C306) and drought-sensitive (Triticum aestivum Cv. WL711) genotypes, using Affymetrix GeneChip® Wheat Genome Array. Both genotypes exhibited di...

journal_title：Protoplasma

authors： Kumar J,Gunapati S,Kianian SF,Singh SP

更新日期：2018-09-01 00:00:00

abstract：:The actin cytoskeleton is involved in the positioning of statoliths in tip growing Chara rhizoids. The balance between the acropetally acting gravity force and the basipetally acting net outcome of cytoskeletal force results in the dynamically stable position of the statoliths 10-30 micrometers above the cell tip. A c...

journal_title：Protoplasma

authors： Braun M,Sievers A

更新日期：1993-01-01 00:00:00

abstract：:In a typical cell division, chromosomes align at the metaphase plate before anaphase commences. This is not the case in Mesostoma spermatocytes. Throughout prometaphase, the three bivalents persistently oscillate towards and away from either pole, at average speeds of 5-6 μm/min, without ever aligning at a metaphase p...

journal_title：Protoplasma

authors： Fegaras E,Forer A

更新日期：2018-07-01 00:00:00

abstract：:In the present study energy-filtering transmission electron microscopy by use of an in-column spectrometer is employed as a powerful tool for ultrastructural analysis of plant cells. Images of unstained very thin (50 nm) and thick (140 nm) sections of the unicellular green alga Micrasterias denticulata, as a model sys...

journal_title：Protoplasma

authors： Lütz-Meindl U,Aichinger N

更新日期：2004-06-01 00:00:00

abstract：:Octanal is a potential alternative to chemical fungicides in controlling postharvest disease of citrus fruit. In this study, the antifungal activity and the underlying mechanism of octanal against spore germination of Penicillium digitatum, one of the main postharvest pathogens in citrus, were investigated. Results sh...

journal_title：Protoplasma

authors： Dou S,Liu S,Xu X,OuYang Q,Tao N

更新日期：2017-07-01 00:00:00

abstract：:Understanding the mechanisms that microbes exploit to invade host cells and cause disease is crucial if we are to eliminate their threat. Although pathogens use a variety of microbial factors to trigger entry into non-phagocytic cells, their targeting of the host cell process of endocytosis has emerged as a common the...

journal_title：Protoplasma

authors： Lin AE,Guttman JA

更新日期：2010-08-01 00:00:00

abstract：:This paper reports an efficient protoplast culture technique, the "extra thin alginate film" technique. The development of this improved method of protoplast culture was an outcome of an assessment of the efficiency and shortcomings of various protoplast culture techniques. The efficiency of this technique was evaluat...

journal_title：Protoplasma

authors： Pati PK,Sharma M,Ahuja PS

更新日期：2005-12-01 00:00:00