Ultrastructural analysis of flagellar development in plurilocular sporangia of Ectocarpus siliculosus (Phaeophyceae).

Abstract:

:Flagellar development in the plurilocular zoidangia of sporophytes of the brown alga Ectocarpus siliculosus was analyzed in detail using transmission electron microscopy and electron tomography. A series of cell divisions in the plurilocular zoidangia produced the spore-mother cells. In these cells, the centrioles differentiated into flagellar basal bodies with basal plates at their distal ends and attached to the plasma membrane. The plasma membrane formed a depression (flagellar pocket) into where the flagella elongated and in which variously sized vesicles and cytoplasmic fragments accumulated. The anterior and posterior flagella started elongating simultaneously, and the vesicles and cytoplasmic fragments in the flagellar pocket fused to the flagellar membranes. The two flagella (anterior and posterior) could be clearly distinguished from each other at the initial stage of their development by differences in length, diameter and the appendage flagellar rootlets. Flagella continued to elongate in the flagellar pocket and maintained their mutually parallel arrangement as the flagellar pocket gradually changed position. In mature zoids, the basal part of the posterior flagellum (paraflagellar body) characteristically became swollen and faced the eyespot region. Electron dense materials accumulated between the axoneme and the flagellar membrane, and crystallized materials could also be observed in the swollen region. Before liberation of the zoospores from the plurilocular zoidangia, mastigoneme attachment was restricted to the distal region of the anterior flagellum. Structures just below the flagellar membrane that connected to the mastigonemes were clearly visible by electron tomography.

journal_name

Protoplasma

journal_title

Protoplasma

authors

Fu G,Nagasato C,Ito T,Müller DG,Motomura T

doi

10.1007/s00709-012-0405-7

subject

Has Abstract

pub_date

2013-02-01 00:00:00

pages

261-72

issue

1

eissn

0033-183X

issn

1615-6102

journal_volume

250

pub_type

杂志文章
  • Advances in Boerhaavia diffusa hairy root technology: a valuable pursuit for identifying strain sensitivity and up-scaling factors to refine metabolite yield and bioactivity potentials.

    abstract::The present study reports the Agrobacterium rhizogenes-mediated hairy root induction in of an ethno-medicinally significant herb-Boerhaavia diffusa L., for elucidating the underlying competence regarding its biosynthetic (i.e. boeravinone B and eupalitin) and bioactivity (antibacterial, antioxidant and anti-inflammato...

    journal_title:Protoplasma

    pub_type: 杂志文章

    doi:10.1007/s00709-015-0875-5

    authors: Gupta R,Pandey P,Singh S,Singh DK,Saxena A,Luqman S,Bawankule DU,Banerjee S

    更新日期:2016-07-01 00:00:00

  • Molecular diffusion and binding analyzed with FRAP.

    abstract::Intracellular molecular transport and localization are crucial for cells (plant cells as much as mammalian cells) to proliferate and to adapt to diverse environmental conditions. Here, some aspects of the microscopy-based method of fluorescence recovery after photobleaching (FRAP) are introduced. In the course of the ...

    journal_title:Protoplasma

    pub_type: 杂志文章,评审

    doi:10.1007/s00709-013-0604-x

    authors: Wachsmuth M

    更新日期:2014-03-01 00:00:00

  • Comparison between the effects of fusicoccin, Tunicamycin, and Brefeldin A on programmed cell death of cultured sycamore (Acer pseudoplatanus L.) cells.

    abstract::Programmed cell death occurs in plants during several developmental processes and during the expression of resistance to pathogen attack (i.e., the hypersensitive response). An unsolved question of plant programmed cell death is whether a unique signaling pathway or different, possibly convergent pathways exist. This ...

    journal_title:Protoplasma

    pub_type: 杂志文章

    doi:10.1007/s00709-004-0053-7

    authors: Malerba M,Cerana R,Crosti P

    更新日期:2004-10-01 00:00:00

  • Protective effect of glutamine on the main and adjacent organs damaged by ischemia-reperfusion in rats.

    abstract::Intestinal ischemia and reperfusion (I/R) causes cellular and tissue damage to the intestine and remote organs such as the liver. Increased production of ROS and nitric oxide and dysregulation of cytoprotective enzymes may be involved in intestinal I/R. The aim was to evaluate the protective effects of glutamine on th...

    journal_title:Protoplasma

    pub_type: 杂志文章

    doi:10.1007/s00709-017-1102-3

    authors: Hartmann RM,Licks F,Schemitt EG,Colares JR,do Couto Soares M,Zabot GP,Fillmann HS,Marroni NP

    更新日期:2017-11-01 00:00:00

  • Fungal hypaphorine reduces growth and induces cytosolic calcium increase in root hairs of Eucalyptus globulus.

    abstract::Root hairs are tubular cells resulting from a tip-localized growth in which calcium ions play a key role. Hypaphorine, an indole alkaloid secreted by the fungus Pisolithus microcarpus during the formation of ectomycorrhizae with the host plant Eucalyptus globulus, inhibits root hair tip growth. Hypaphorine-induced inh...

    journal_title:Protoplasma

    pub_type: 杂志文章

    doi:10.1007/s00709-006-0240-9

    authors: Dauphin A,Gérard J,Lapeyrie F,Legué V

    更新日期:2007-01-01 00:00:00

  • Crosstalk of cell polarity signaling pathways.

    abstract::Cell polarity, the asymmetric organization of cellular components along one or multiple axes, is present in most cells. From budding yeast cell polarization induced by pheromone signaling, oocyte polarization at fertilization to polarized epithelia and neuronal cells in multicellular organisms, similar mechanisms are ...

    journal_title:Protoplasma

    pub_type: 杂志文章,评审

    doi:10.1007/s00709-017-1075-2

    authors: Mazel T

    更新日期:2017-05-01 00:00:00

  • Cytoarchitecture of the desiccation-tolerant green alga Zygogonium ericetorum.

    abstract::In this study, the filamentous green alga Zygogonium ericetorum (Zygnematales, Chlorophyta), collected at its natural habitat in the high alps, was investigated by light, scanning, and transmission electron microscopy. The field samples were separated into a moist fraction when wetted by splattering water of a nearby ...

    journal_title:Protoplasma

    pub_type: 杂志文章

    doi:10.1007/s00709-009-0048-5

    authors: Holzinger A,Tschaikner A,Remias D

    更新日期:2010-07-01 00:00:00

  • Effect of Rho family GTP-binding proteins on Amoeba proteus.

    abstract::While there is a number of studies on the effects of Rho GTPases on the actin-based cytoskeleton in higher eukaryotes, studies in protozoans are rather limited. The problem seems to be intriguing since the structure of protozoan cytoskeletons is distinct from most vertebrate cells. By blocking endogenous Rho family pr...

    journal_title:Protoplasma

    pub_type: 杂志文章

    doi:10.1007/s00709-002-0040-9

    authors: Kłopocka W,Redowicz MJ

    更新日期:2003-03-01 00:00:00

  • Ultrastructural aspects of pollen ontogeny in an endangered plant species, Pancratium maritimum L. (Amaryllidaceae).

    abstract::Pollen ontogeny in Pancratium maritimum L. was studied from the sporogenous cell to mature pollen grain stages using transmission electron, scanning electron, and light microscopy to determine whether the pollen development in P. maritimum follows the basic scheme in angiosperms or not. In the course of microsporogene...

    journal_title:Protoplasma

    pub_type: 杂志文章

    doi:10.1007/s00709-016-0998-3

    authors: Tütüncü Konyar S

    更新日期:2017-03-01 00:00:00

  • Chromosomes selectively detach at one pole and quickly move towards the opposite pole when kinetochore microtubules are depolymerized in Mesostoma ehrenbergii spermatocytes.

    abstract::In a typical cell division, chromosomes align at the metaphase plate before anaphase commences. This is not the case in Mesostoma spermatocytes. Throughout prometaphase, the three bivalents persistently oscillate towards and away from either pole, at average speeds of 5-6 μm/min, without ever aligning at a metaphase p...

    journal_title:Protoplasma

    pub_type: 杂志文章

    doi:10.1007/s00709-018-1214-4

    authors: Fegaras E,Forer A

    更新日期:2018-07-01 00:00:00

  • Unusual microtubular cytoskeleton of apomictic embryo sac of Chondrilla juncea L.

    abstract::The mature apomictic embryo sac of Chondrilla juncea is highly vacuolated and demonstrates a polarization similar to that of the amphimictic gametophyte. The microtubule cytoskeleton of this embryo sac is uncharacteristic and relatively weak. The microtubules are positioned along cell walls and resemble cortical micro...

    journal_title:Protoplasma

    pub_type: 杂志文章

    doi:10.1007/s00709-006-0147-5

    authors: Kościńska-Pajak M,Bednara J

    更新日期:2006-05-01 00:00:00

  • Pollen wall ontogeny in Polemonium caeruleum (Polemoniaceae) and suggested underlying mechanisms of development.

    abstract::By a detailed ontogenetic study of Polemonium caeruleum pollen, tracing each stage of development at high TEM resolution, we aim to understand the establishment of the pollen wall and to unravel the mechanisms underlying sporoderm development. The main steps of exine ontogeny in Polemonium caeruleum, observed in the m...

    journal_title:Protoplasma

    pub_type: 杂志文章

    doi:10.1007/s00709-017-1121-0

    authors: Grigorjeva VV,Gabarayeva N

    更新日期:2018-01-01 00:00:00

  • Comparative biochemical characterization of peroxidases (class III) tightly bound to the maize root cell walls and modulation of the enzyme properties as a result of covalent binding.

    abstract::Comparative biochemical characterization of class III peroxidase activity tightly bound to the cell walls of maize roots was performed. Ionically bound proteins were solubilized from isolated walls by salt washing, and the remaining covalently bound peroxidases were released, either by enzymatic digestion or by a nove...

    journal_title:Protoplasma

    pub_type: 杂志文章

    doi:10.1007/s00709-014-0684-2

    authors: Hadži-Tašković Šukalović V,Vuletić M,Marković K,Cvetić Antić T,Vučinić Ž

    更新日期:2015-01-01 00:00:00

  • Critically reduced frost resistance of Picea abies during sprouting could be linked to cytological changes.

    abstract::Frost resistance of sprouting Picea abies shoots is insufficient for survival of naturally occurring late frosts. The cellular changes during sprouting appeared to be responsible for frost damage as frost events that damaged sprouting shoots did not damage older needles and stems. Whilst resting buds showed initial fr...

    journal_title:Protoplasma

    pub_type: 杂志文章

    doi:10.1007/s00709-009-0052-9

    authors: Neuner G,Beikircher B

    更新日期:2010-07-01 00:00:00

  • Cytological cycles and fates in Psidium myrtoides are altered towards new cell metabolism and functionalities by the galling activity of Nothotrioza myrtoidis.

    abstract::The morphogenesis of galls occurs by the redifferentiation of cells that assume new functions in the modified host plant organs. The redifferentiated cells in the galls of Nothotrioza myrtoidis on Psidium myrtoides have low complexity metabolism and are photosynthesis-deficient. These galls were studied in search for ...

    journal_title:Protoplasma

    pub_type: 杂志文章

    doi:10.1007/s00709-014-0709-x

    authors: Carneiro RG,Isaias RM

    更新日期:2015-03-01 00:00:00

  • Covisualization by computational optical-sectioning microscopy of integrin and associated proteins at the cell membrane of living onion protoplasts.

    abstract::Using higher-resolution wide-field computational optical-sectioning fluorescence microscopy, the distribution of antigens recognized by antibodies against animal beta 1 integrin, fibronectin, and vitronectin has been visualized at the outer surface of enzymatically protoplasted onion epidermis cells and in depectinate...

    journal_title:Protoplasma

    pub_type: 杂志文章

    doi:10.1007/BF01882029

    authors: Gens JS,Reuzeau C,Doolittle KW,McNally JG,Pickard BG

    更新日期:1996-01-01 00:00:00

  • A promising approach on biomass accumulation and withanolides production in cell suspension culture of Withania somnifera (L.) Dunal.

    abstract::Withanolide is one of the most extensively exploited steroidal lactones, which are biosynthesized in Withania somnifera. Its production from cell suspension culture was analyzed to defeat limitations coupled with its regular supply from the plant organs. In order to optimize the different factors for sustainable produ...

    journal_title:Protoplasma

    pub_type: 杂志文章

    doi:10.1007/s00709-012-0471-x

    authors: Sivanandhan G,Kapil Dev G,Jeyaraj M,Rajesh M,Muthuselvam M,Selvaraj N,Manickavasagam M,Ganapathi A

    更新日期:2013-08-01 00:00:00

  • Multivesicular bodies differentiate exclusively in nutritive fast-dividing cells in Marcetia taxifolia galls.

    abstract::Marcetia taxifolia (A. St.-Hil.) DC. hosts two gall morphotypes, a pistil-shaped gall induced by a Cecidomyiidae (Diptera) and a fusiform stem gall induced by a Lepidoptera. The cytological study of these galls aimed to answer how the difference in nutritive tissues of Diptera and Lepidoptera galls could be explained ...

    journal_title:Protoplasma

    pub_type: 杂志文章

    doi:10.1007/s00709-015-0759-8

    authors: Ferreira BG,Carneiro RG,Isaias RM

    更新日期:2015-09-01 00:00:00

  • Gibberellin and the plant growth retardant Paclobutrazol altered fruit shape and ripening in tomato.

    abstract::Fruit shape and ripening are major horticultural traits for many fruits and vegetable crops. Changes in fruit shape and ripening are often accomplished by altered cell division or cell expansion patterns. Gibberellic acids (GAs) are essential for tomato fruit development; however, the exact role and the underlying mec...

    journal_title:Protoplasma

    pub_type: 杂志文章

    doi:10.1007/s00709-019-01471-2

    authors: Chen S,Wang XJ,Tan GF,Zhou WQ,Wang GL

    更新日期:2020-05-01 00:00:00

  • The garlic NF-YC gene, AsNF-YC8, positively regulates non-ionic hyperosmotic stress tolerance in tobacco.

    abstract::To investigate the relationship between nuclear factor Y (NF-Y) and stress tolerance in garlic, we cloned a NF-Y family gene AsNF-YC8 from garlic, which was largely upregulated at dehydrate stage. Expression pattern analyses in garlic revealed that AsNF-YC8 is induced through abscisic acid (ABA) and abiotic stresses, ...

    journal_title:Protoplasma

    pub_type: 杂志文章

    doi:10.1007/s00709-016-1026-3

    authors: Sun X,Lian H,Liu X,Zhou S,Liu S

    更新日期:2017-05-01 00:00:00

  • Differential effects of two phospholipase D inhibitors, 1-butanol and N-acylethanolamine, on in vivo cytoskeletal organization and Arabidopsis seedling growth.

    abstract::Plant development is regulated by numerous chemicals derived from a multitude of metabolic pathways. However, we know very little about the biological effects and functions of many of these metabolites in the cell. N-Acylethanolamines (NAEs) are a group of lipid mediators that play important roles in mammalian physiol...

    journal_title:Protoplasma

    pub_type: 杂志文章

    doi:10.1007/s00709-005-0124-4

    authors: Motes CM,Pechter P,Yoo CM,Wang YS,Chapman KD,Blancaflor EB

    更新日期:2005-12-01 00:00:00

  • Exposure of Vicia faba and Pisum sativum to copper-induced genotoxicity.

    abstract::The potential genotoxicity of Cu(2+) was investigated in Vicia faba and Pisum sativum seedlings in hydroponic culture conditions. Cu(2+) caused a dose-dependent increase in micronuclei frequencies in both plant models. Cytological analysis of root tips cells showed clastogenic and aneugenic effects of this heavy metal...

    journal_title:Protoplasma

    pub_type: 杂志文章

    doi:10.1007/s00709-008-0004-9

    authors: Souguir D,Ferjani E,Ledoigt G,Goupil P

    更新日期:2008-11-01 00:00:00

  • Pre-meiotic bands and novel meiotic spindle ontogeny in quadrilobed sporocytes of leafy liverworts (Jungermannidae, Bryophyta).

    abstract::Indirect immunofluorescence and confocal microscopy were used to study the nucleation and organization of microtubules during meiosis in two species of leafy liverworts, Cephalozia macrostachya and Telaranea longifolia. This is the first such study of sporogenesis in the largest group of liverworts important as living...

    journal_title:Protoplasma

    pub_type: 杂志文章

    doi:10.1007/s00709-009-0073-4

    authors: Brown RC,Lemmon BE

    更新日期:2009-10-01 00:00:00

  • What generates flux of tubulin in kinetochore microtubules?

    abstract::We discuss models for production of tubulin flux in kinetochore microtubules. Current models concentrate solely on microtubules and their associated motors and enzymes. For example, in some models the driving force for flux is enzymes at the poles and the kinetochores; in others the driving force is motor molecules th...

    journal_title:Protoplasma

    pub_type: 杂志文章

    doi:10.1007/s00709-008-0286-y

    authors: Forer A,Pickett-Heaps JD,Spurck T

    更新日期:2008-01-01 00:00:00

  • A possible role for C4 photosynthetic enzymes in tolerance of Zea mays to NaCl.

    abstract::Treatment of 14-day-old maize cultivars (Hybrid351 and Giza2) with 250 mM NaCl significantly reduced shoot fresh and dry weights and protein content during the subsequent 12 days. The magnitude of reduction was more pronounced in Giza than Hybrid. Both cultivars contained converging levels of protein for the enzymes p...

    journal_title:Protoplasma

    pub_type: 杂志文章

    doi:10.1007/s00709-011-0356-4

    authors: Alla MM,Hassan NM

    更新日期:2012-10-01 00:00:00

  • Changes in plasma membrane fluidity of corn (Zea mays L.) roots after Brij 58 treatment.

    abstract::The detergent Brij 58 has been introduced to reverse plasma membrane (PM) vesicles from the right-side-out to the inside-out form. The aim of the present work was to investigate the effect of Brij 58 on the formation of an ATP-dependent proton gradient and on the fluidity of the lipid phase of PM vesicles. PMs of corn...

    journal_title:Protoplasma

    pub_type: 杂志文章

    doi:10.1007/BF01289415

    authors: Behzadipour M,Kluge M,Lüthje S

    更新日期:2001-01-01 00:00:00

  • Characterization of surface sugars on algal cells with fluorescein isothiocyanate-conjugated lectins.

    abstract::We used qualitative and quantitative fluorescence microscopy of the fluorescein isothiocyanate-conjugated lectins Concanavalin A, phytohaemagglutinin-erythroagglutinin, pokeweed mitogen, and peanut agglutinin to examine sugar composition on the cell surface and cell-associated mucilage (where present) in a number of c...

    journal_title:Protoplasma

    pub_type: 杂志文章

    doi:10.1007/s00709-005-0092-8

    authors: Tien CJ,Sigee DC,White KN

    更新日期:2005-10-01 00:00:00

  • Callose and homogalacturonan epitope distribution in stomatal complexes of Zea mays and Vigna sinensis.

    abstract::This article deals with the distribution of callose and of the homogalacturonan (HG) epitopes recognized by LM20, JIM5, and 2F4 antibodies in cell walls of differentiating and functioning stomatal complexes of the monocotyledon Zea mays and the dicotyledon Vigna sinensis. The findings revealed that, during stomatal de...

    journal_title:Protoplasma

    pub_type: 杂志文章

    doi:10.1007/s00709-019-01425-8

    authors: Giannoutsou E,Sotiriou P,Nikolakopoulou TL,Galatis B,Apostolakos P

    更新日期:2020-01-01 00:00:00

  • Effect of mercury on pollen germination and tube growth in Lilium longiflorum.

    abstract::Pollen development and germination were adversely affected by the presence of mercury, whereas low-concentrations stimulated the whole procedure. Mercury caused morphological anomalies during the tube growth, characterized by irregularly increasing diameters and swelling tips. The main effect was the anomalous cell wa...

    journal_title:Protoplasma

    pub_type: 杂志文章

    doi:10.1007/s00709-017-1192-y

    authors: Sawidis T,Baycu G,Cevahir-Öz G,Weryszko-Chmielewska E

    更新日期:2018-05-01 00:00:00

  • ALTERED MERISTEM PROGRAM 1 promotes growth and biomass accumulation influencing guard cell aperture and photosynthetic efficiency in Arabidopsis.

    abstract::ALTERED MERISTEM PROGRAM 1 (AMP1) encodes a putative glutamate-carboxypeptidase important for plant growth and development. In this study, by comparing the growth of Arabidopsis wild-type, amp1-10 and amp1-13 mutants, and AMP1-GFP/OX2- and AMP1-GFP/OX7-overexpressing seedlings in vitro and in soil, we uncover the role...

    journal_title:Protoplasma

    pub_type: 杂志文章

    doi:10.1007/s00709-019-01458-z

    authors: López-García CM,Ruíz-Herrera LF,López-Bucio JS,Huerta-Venegas PI,Peña-Uribe CA,de la Cruz HR,López-Bucio J

    更新日期:2020-03-01 00:00:00