Performance and suitability of polymerase chain reaction for early detection of bacteria in platelet concentrates.

Abstract:

BACKGROUND:In this study the applicability of a 16S rRNA real-time reverse transcriptase polymerase chain reaction (RT-PCR) and a Staphylococcus genus-specific PCR for screening of bacterial contamination in platelet concentrates (PCs) was determined. STUDY DESIGN AND METHODS:A total of 336 sample bags, from PCs that were routinely tested in the BacT/ALERT (bioMérieux), were collected and frozen until testing by the PCR assays. Based on the BacT/ALERT results, 107 PCs were positive and 229 were negative for bacterial growth. RESULTS:The analytical sensitivity of the 16S rRNA real-time RT-PCR ranged from 5 to 40 colony-forming units (CFUs)/mL. The PCR detected five positive samples, four of which were also positive in the BacT/ALERT. The sensitivity of the test was 3.8%, and the specificity was 99.5%. The analytical sensitivity of the Staphylococcus genus-specific PCR ranged from 5 to 15 CFUs/mL. Thirty-nine units that were BacT/ALERT positive for staphylococci were tested with this PCR. Six samples were positive with the PCR, five of which were also BacT/ALERT positive. The sensitivity of the Staphylococcus genus-specific PCR was 12.8%, and the specificity was 98.8%. CONCLUSION:Despite the rapid availability of results compared to the BacT/ALERT, the analytical sensitivity of a generic or specific PCR assay is not high enough to be an alternative for the BacT/ALERT when PCs are screened on the day of production.

journal_name

Transfusion

journal_title

Transfusion

authors

Rood IG,Pettersson A,Savelkoul PH,de Korte D

doi

10.1111/j.1537-2995.2011.03090.x

subject

Has Abstract

pub_date

2011-09-01 00:00:00

pages

2006-11

issue

9

eissn

0041-1132

issn

1537-2995

journal_volume

51

pub_type

杂志文章
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    pub_type: 杂志文章

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    更新日期:2018-01-01 00:00:00

  • Enhanced HPC recruitment in children using LVL and a new automated apheresis system.

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    doi:10.1046/j.1537-2995.2000.40040404.x

    authors: Torrabadella M,Olivé T,Ortega JJ,Massuet L

    更新日期:2000-04-01 00:00:00

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    pub_type: 杂志文章

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    authors: Bertolini F,Murphy S,Rebulla P,Sirchia G

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    pub_type: 杂志文章,多中心研究,随机对照试验

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  • Murine monoclonal antibodies associated with Rh17, Rh29, and Rh46 antigens.

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    pub_type: 杂志文章

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    authors: Rouger P,Edelman L

    更新日期:1988-01-01 00:00:00

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    pub_type: 杂志文章

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    更新日期:1991-07-01 00:00:00

  • The effects of combined platelet and leukapheresis on the blood coagulation system.

    abstract::Analysis of blood coagulation was done on samples of blood collected from ten donors undergoing combined platelet and leukapheresis using the Haemonetics Model 30 Blood Processor. Blood samples were obtained from the donors prior to, during, and following pheresis. Blood was also obtained from the blood-return line af...

    journal_title:Transfusion

    pub_type: 杂志文章

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    authors: Kisker CT,Strauss RG,Koepke JA,Maguire LC,Thompson JS

    更新日期:1979-03-01 00:00:00

  • Treatment of an acquired Factor XIII inhibitor in an adolescent with systemic lupus erythematosus and renal failure.

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    pub_type:

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    authors: Rabik CA,Atkinson MA,Sule S,Strouse JJ

    更新日期:2017-09-01 00:00:00

  • The symbolic roots of blood donation.

    abstract:BACKGROUND:Throughout history, blood has been embedded with powerful and paradoxical connotations. In the last decades, a more rational conception based on scientific knowledge has emerged, portraying blood as a "disenchanted" and utilitarian object, hence more apt for donation between strangers. This study aims to exa...

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    pub_type: 杂志文章

    doi:10.1111/trf.12477

    authors: Charbonneau J,Tran NY

    更新日期:2013-12-01 00:00:00

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    pub_type: 杂志文章,随机对照试验

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    authors: Hanson SA,France CR

    更新日期:2009-05-01 00:00:00

  • The Brazilian experience of nucleic acid testing to detect human immunodeficiency virus, hepatitis C virus, and hepatitis B virus infections in blood donors.

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    pub_type: 杂志文章

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    authors: Rocha D,Andrade E,Godoy DT,Fontana-Maurell M,Costa E,Ribeiro M,Ferreira AGP,Brindeiro R,Tanuri A,Alvarez P

    更新日期:2018-04-01 00:00:00

  • Hematology patterns after hemoglobin-based oxygen carrier resuscitation from severe controlled hemorrhage with prolonged delayed definitive care.

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    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/j.1537-2995.2007.01435.x

    authors: Arnaud F,Fasipe D,Philbin N,Rice J,Flournoy WS,Ahlers S,McCarron R,Freilich D

    更新日期:2007-11-01 00:00:00

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    pub_type: 杂志文章

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    更新日期:1989-06-01 00:00:00

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    journal_title:Transfusion

    pub_type: 杂志文章,评审

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    authors: Łętowska M,Przybylska Z,Piotrowski D,Lachert E,Rosiek A,Rzymkiewicz L,Cardoso M

    更新日期:2016-03-01 00:00:00

  • Prevalence and quantitation of parvovirus B19 DNA levels in blood donors with a sensitive polymerase chain reaction screening assay.

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    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/j.1537-2995.2007.01341.x

    authors: Kleinman SH,Glynn SA,Lee TH,Tobler L,Montalvo L,Todd D,Kiss JE,Shyamala V,Busch MP,National Heart, Lung, Blood Institute Retrovirus Epidemiology Donor Study (REDS-II).

    更新日期:2007-10-01 00:00:00

  • In vitro safety profile of G-CSF-mobilized whole blood after storage for 7 days in an infusable-grade L15 medium.

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    pub_type: 杂志文章

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    更新日期:2002-04-01 00:00:00

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    journal_title:Transfusion

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    authors: Staples S,O'Callaghan C,Pavord S,Staves J,Murphy MF

    更新日期:2020-09-01 00:00:00

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    journal_title:Transfusion

    pub_type: 杂志文章

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    authors: Atance R,Pereira A,Ramírez B

    更新日期:2001-12-01 00:00:00

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    journal_title:Transfusion

    pub_type: 杂志文章

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    更新日期:1977-07-01 00:00:00

  • Long-term production of pre-existing alloantibodies to E and c after allogenic BMT in a patient with aplastic anemia resulting in delayed hemolytic anemia.

    abstract:BACKGROUND:Delayed hemolysis mediated by long-term production of pre-existing recipient-derived antibodies directed against donor RBC antigens after allogenic BMT is an unusual complication of hematopoietic transplantation. CASE REPORT:A 26-year-old man with aplastic anemia had pre-existing alloantibodies to E and c. ...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.2003.00303.x

    authors: Izumi N,Fuse I,Furukawa T,Uesugi Y,Tsuchiyama J,Toba K,Togashi K,Yamada K,Ohtake S,Saitoh Y,Yanagisawa N,Aizawa Y

    更新日期:2003-02-01 00:00:00

  • Prevalence, incidence, and residual risk of human immunodeficiency virus and hepatitis C virus infections among United States blood donors since the introduction of nucleic acid testing.

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    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/j.1537-2995.2010.02622.x

    authors: Zou S,Dorsey KA,Notari EP,Foster GA,Krysztof DE,Musavi F,Dodd RY,Stramer SL

    更新日期:2010-07-01 00:00:00

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    journal_title:Transfusion

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1046/j.1537-2995.2001.41081064.x

    authors: Jaeger K,Heine J,Ruschulte H,Jüttner B,Scheinichen D,Kuse ER,Piepenbrock S

    更新日期:2001-08-01 00:00:00

  • A chloroquine elution technique for platelet serology.

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    pub_type: 杂志文章

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    authors: Masel DS,Blumberg N,Heal JM

    更新日期:1988-03-01 00:00:00

  • Antibodies to co-trimoxazole (trimethoprim and/or sulfamethoxazole) related to the presence of the drug in a commercial low-ionic-strength solution.

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    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/j.1537-2995.2011.03327.x

    authors: Pham BN,Gien D,Bensaad F,Babinet J,Dubeaux I,Rouger P,Le Pennec PY

    更新日期:2012-04-01 00:00:00

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    pub_type: 杂志文章

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    authors: Meryman HT,Hornblower M

    更新日期:1977-09-01 00:00:00

  • Comparative evaluation of supplemental hepatitis C virus antibody test systems.

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    pub_type: 杂志文章

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    authors: Evans CS,Tobler L,Polito A,Stewart J,Chien D,Wilber J,Quan S,Delaney S,Kuo G,Busch MP

    更新日期:1992-06-01 00:00:00

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    doi:10.1111/j.1537-2995.2011.03450.x

    authors: Dodd RY,Hackett J Jr,Linnen JM,Dorsey K,Wu Y,Zou S,Qiu X,Swanson P,Schochetman G,Gao K,Carrick JM,Krysztof DE,Stramer SL

    更新日期:2012-02-01 00:00:00

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    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/trf.14264

    authors: Jhang JS,Francis RO,Winkler A,Tormey C

    更新日期:2017-10-01 00:00:00

  • The risk of transfusion-transmitted infections in sub-Saharan Africa.

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    journal_title:Transfusion

    pub_type: 杂志文章,评审

    doi:10.1111/j.1537-2995.2009.002402.x

    authors: Jayaraman S,Chalabi Z,Perel P,Guerriero C,Roberts I

    更新日期:2010-02-01 00:00:00

  • Coagulation parameters of CPD fresh-frozen plasma and CPD cryoprecipitate-poor plasma after storage at 4 degrees C for 28 days.

    abstract::A pilot study was performed on the storage of plasma and cryosupernatant plasma at 4 degrees C for up to 28 days. Eight bags, four of CPD fresh-frozen plasma (FFP) and four of CPD cryosupernatant plasma (CSP, plasma without cryoprecipitate), were sampled during storage for assays of pH; factors V, VIII, IX, and XI; fi...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1993.33994025023.x

    authors: Smak Gregoor PJ,Harvey MS,Briët E,Brand A

    更新日期:1993-09-01 00:00:00