Effects of colloidal resuscitation fluids on the neutrophil respiratory burst.

Abstract:

BACKGROUND:Experimental studies have revealed that gelatin and HES produce increased neutrophil respiratory burst activity. It was investigated whether 3-percent gelatin (MW 35,000) and three types of 6-percent HES (MW 70,000; degree of substitution, 0.5; 200,000/0.5; 450,000/0.7) preparations can influence superoxide anion production during respiratory burst under clinical conditions. STUDY DESIGN AND METHODS:Blood samples were obtained from 40 patients before and 1 hour after the infusion, before anesthesia and surgical treatment. After stimulation with bacteria (Escherichia coli), the respiratory burst was measured by oxidation of nonfluorescent dihydrorhodamine 123 to the fluorescent rhodamine 123 by the use of flow cytometry. RESULTS:Respiratory burst activity decreased significantly (p = 0.004) from the baseline (60.0 +/- 6.5%) to 1 hour after the administration of the low-molecular-weight HES preparation (55.0 +/- 6.8%). No significant differences in respiratory burst activity could be found after the administration of gelatin or medium-molecular-weight or high- molecular-weight HES solution. CONCLUSION:The investigated administration of gelatin and medium- and high-molecular-weight HES preparations did not influence respiratory burst activity under clinical conditions. However, the neutrophil respiratory burst was impaired after the administration of low-molecular-weight HES. Neutrophil respiratory burst activity may vary according to the type of colloidal plasma substitutes administered.

journal_name

Transfusion

journal_title

Transfusion

authors

Jaeger K,Heine J,Ruschulte H,Jüttner B,Scheinichen D,Kuse ER,Piepenbrock S

doi

10.1046/j.1537-2995.2001.41081064.x

subject

Has Abstract

pub_date

2001-08-01 00:00:00

pages

1064-8

issue

8

eissn

0041-1132

issn

1537-2995

journal_volume

41

pub_type

临床试验,杂志文章,随机对照试验
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    pub_type: 杂志文章

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    pub_type: 临床试验,杂志文章,随机对照试验

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    pub_type: 杂志文章

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