Abstract:
BACKGROUND:In the past 10 years, PCR-based methods have been described to allow the detection of gene polymorphisms responsible for many blood group antigens. These methods are routinely used to test samples of fetal origin and to resolve serologic discrepancies. Another interesting application of blood group genotyping could be the extended typing of blood donors for minor antigens to facilitate the procurement of compatible blood for alloimmunized patients. STUDY DESIGN AND METHODS:PCR-based tests have been modified to allow multiplex amplification of specific fragments of blood group genes and the convenient detection of hybridized amplicons by ELISA in a microplate format. RESULTS:The results obtained show that fragments of the Rh (D, c, C, e, E), Kell (K, k), Duffy (Fya, Fyb), and Kidd (Jka, Jkb) genes could be amplified along with controls in multiplex PCR reactions. Labeling of amplicons with digoxigenin allowed their solid-phase detection in microplate wells previously coated with individual blood group-specific oligonucleotides. A comparative study performed with 100 individuals showed a 99.7 percent concordance between genotypes and phenotypes for the 11 antigens assayed, with only three discrepant Fyb genotypes. CONCLUSION:Extended genotyping could be performed once on regular donors and confirmed when needed by standard serologic RBC assays. The format of these tests will allow easy automation of the procedure including the interpretation and downloading of the results with existing ELISA software.
journal_name
Transfusionjournal_title
Transfusionauthors
St-Louis M,Perreault J,Lemieux Rdoi
10.1046/j.1537-2995.2003.00474.xsubject
Has Abstractpub_date
2003-08-01 00:00:00pages
1126-32issue
8eissn
0041-1132issn
1537-2995pii
474journal_volume
43pub_type
临床试验,杂志文章,随机对照试验相关文献
TRANSFUSION文献大全abstract:BACKGROUND:Historically, paid blood donors were found to transmit hepatitis at higher rates than volunteers. In those older studies, paid donors frequently were recruited from prisons or slum areas--a finding consistent with the belief that monetary payment in itself did not necessarily lead to the high-risk status of ...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1994.34294143937.x
更新日期:1994-02-01 00:00:00
abstract::An exchange transfusion was performed preoperatively on a patient with sickle cell disease using a continuous-flow blood cell separator. An exchange of 2,825 ml red blood cells achieved a hemoglobin A level of 90.8 per cent. The continuous-flow blood cell separator appears to offer a safe and effective method of excha...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1977.17377196364.x
更新日期:1977-05-01 00:00:00
abstract:BACKGROUND:An influx of African migrants and refugees can strain a host country's blood services, because often migrants have unique blood needs that cannot be sourced from local donors. To increase blood donation by the new migrants, host country blood services need to understand how blood and blood donations are view...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2010.03053.x
更新日期:2011-08-01 00:00:00
abstract:BACKGROUND:Beriplex P/N/Kcentra/Coaplex/Confidex is a four-factor human prothrombin complex concentrate (PCC). Here, we describe the pathogen safety profile and biochemical characteristics of an improved manufacturing process that further enhances the virus safety of Beriplex P/N. STUDY DESIGN AND METHODS:Samples of p...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.14010
更新日期:2017-05-01 00:00:00
abstract::The quantity of anti-IgG bound at equilibrium to cell-bound anti-D failed to increase with enzymatic modification of intact red blood cells. This contrasts with results obtained by immunoelectronmicroscopy with unsealed red blood cell ghosts. Ghosts derived from enzyme-modified, anti-D-sensitized intact red blood cell...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1980.20480260268.x
更新日期:1980-07-01 00:00:00
abstract:BACKGROUND:Plasma is commonly used for vitamin K antagonist (VKA) reversal, but observational studies suggest that it is associated with transfusion-related adverse reactions (e.g., volume overload). However, this issue has not previously been addressed in a randomized controlled trial (RCT). STUDY DESIGN AND METHODS:...
journal_title:Transfusion
pub_type: 杂志文章,随机对照试验
doi:10.1111/trf.13191
更新日期:2015-11-01 00:00:00
abstract::The In(Lu) gene, which inhibits the expression of Lutheran blood group antigens by red cells (RBCs), also down-regulates the expression of an 80-kD glycoprotein, In(Lu)-related p80, by both RBCs and a subset of white cells. This study examined the expression of multiple-RBC p80 epitopes by autosomal and X-linked reces...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1988.28588337330.x
更新日期:1988-09-01 00:00:00
abstract::The US Food and Drug Administration (FDA) held a workshop on red blood cell (RBC) product regulatory science on October 6 and 7, 2016, at the Natcher Conference Center on the National Institutes of Health (NIH) Campus in Bethesda, Maryland. The workshop was supported by the National Heart, Lung, and Blood Institute, N...
journal_title:Transfusion
pub_type:
doi:10.1111/trf.14435
更新日期:2018-01-01 00:00:00
abstract:BACKGROUND:The absence of hybrid Rhesus boxes denotes an RHD homozygous status and helps to detect the presence of Dce haplotypes instead of dce. RHCE exon 1 C48, characteristic of RHC alleles, and RHCE exon 5 G733, responsible for VS antigenicity, have been noted in many RHce alleles but it was not clearly established...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2006.01140.x
更新日期:2007-03-01 00:00:00
abstract:BACKGROUND:Qualification of a cord blood bank (CBB) is a complex process that includes evaluation of multiple aspects of donor screening and testing, processing, accreditation and approval by professional cell therapy groups, and results of received cord blood units. The University of Minnesota Medical Center Cell Ther...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.12085
更新日期:2013-10-01 00:00:00
abstract:BACKGROUND:The AABB Clinical Transfusion Medicine Committee (CTMC) compiles an annual synopsis of the published literature covering important developments in the field of transfusion medicine (TM) for the board of director's review. This synopsis is now made available as a manuscript published in TRANSFUSION. STUDY DE...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.15848
更新日期:2020-07-01 00:00:00
abstract::The morphology of granulocytes collected by continuous-flow centrifugation (CFC), discontinuous-flow centrifugation (DFC), and continuous-flow filtration (CFF) was investigated in 18 healthy donors by means of light microscopy and transmission electron microscopy. Light microscopy study of semithin sections of granulo...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1981.21582040813.x
更新日期:1981-09-01 00:00:00
abstract:BACKGROUND:Bacterial contamination or platelet (PLT) metabolism can change the pH of stored PLT concentrates (PCs). Measurement of pH for quality control is currently done on a limited basis. An easy noninvasive method was developed to obtain sequential pH measurements over time, without risking contamination and/or co...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2010.02727.x
更新日期:2010-12-01 00:00:00
abstract:BACKGROUND:A novel nonenveloped single-stranded circular DNA virus (TTV) was recently identified. The prevalence of TTV in blood donors in the United States is, however, still unclear. STUDY DESIGN AND METHODS:Viral DNA was detected in US blood donors from five cities by using two sets of TTV primers: NG059/NG061/NG06...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.2000.40020245.x
更新日期:2000-02-01 00:00:00
abstract::Quality control to detect inadequately deglycerolized red blood cells can be easily and inexpensively accomplished by suspending the deglycerolized cells in either recipient serum or normal saline in the same way in which the routine crossmatch is performed. In vitro hemolysis is readily and consistently apparent when...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1982.22382224955.x
更新日期:1982-05-01 00:00:00
abstract:BACKGROUND:If anti-hepatitis B core antibody testing is not mandated blood donors with occult hepatitis B infection (OBI) may transmit hepatitis B virus (HBV) to a recipient in spite of the use of nucleic acid amplification technology (NAT) or pathogen inactivation (PI). STUDY DESIGN AND METHODS:We developed a model t...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.14050
更新日期:2017-03-01 00:00:00
abstract:BACKGROUND:RBC components collected from donors with sickle cell trait frequently occlude WBC-reduction filters. In vitro, sickle trait RBCs have the potential for sickle Hb (Hb S) polymerization at low oxygen saturations and high Hb concentrations. STUDY DESIGN AND METHOD:To determine if the low pH and high osmolarit...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.2002.00206.x
更新日期:2002-11-01 00:00:00
abstract:BACKGROUND:The factors contributing to RBC agglutination are complex. The RBC cytoskeleton's participation in and contribution to this phenomenon are difficult to separate from those of the plasma membrane. Immunoreactive, cytoskeleton-free, band 3-enriched microvesicles can be generated from normal RBCs. Band 3 has be...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.2000.40050566.x
更新日期:2000-05-01 00:00:00
abstract:BACKGROUND:Recently, long-standing elevations of soluble growth factors released from platelets (PLTs) after contact with artificial surfaces during dialysis were described. They could be jointly responsible for the high frequency of death from cardiovascular diseases in dialysis patients. There are no comparable data ...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2005.04269.x
更新日期:2005-03-01 00:00:00
abstract::Before the advent of electronic means of communication, postal stamps were one of the main international promotion tools on the planet and still remains an important support for the wide diffusion of a message. Since 1942, the promotion of blood donation has been one of them. To highlight these recurring or specific c...
journal_title:Transfusion
pub_type: 杂志文章,评审
doi:10.1111/j.1537-2995.2010.02671.x
更新日期:2010-08-01 00:00:00
abstract:BACKGROUND:Chronic transfusion in sickle cell disease (SCD) remains the gold standard therapy for stroke prevention and for patients with severe disease despite adequate hydroxyurea treatment. The aim of our study was to assess the safety and efficacy of automated red blood cell exchange (aRBX) in patients with SCD pre...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.14575
更新日期:2018-06-01 00:00:00
abstract:BACKGROUND:By NAT, HBV DNA is occasionally detectable in blood donors with past HBV infection but negative for HBsAg. Whether or not these donors can cause transfusion-transmitted HBV infections is uncertain. STUDY DESIGN AND METHODS:To determine whether or not donors with past HBV infection but negative for HbsAg can...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.2002.00274.x
更新日期:2002-12-01 00:00:00
abstract:BACKGROUND:Unexpected antibodies are frequently associated with hemolytic disease of the fetus and newborn (HDFN) and hemolytic transfusion reactions (HTRs), and screening for those unexpected antibodies is critical for the safety and effectiveness of transfusion. Different populations differ in the prevalence of signi...
journal_title:Transfusion
pub_type: 杂志文章,评审
doi:10.1111/trf.13430
更新日期:2016-04-01 00:00:00
abstract:BACKGROUND:Sickle cell disease (SCD) is associated with hematologic complications including delayed hemolytic transfusion reactions (DHTRs) and pregnancy-related morbidity and mortality. Hyperhemolysis syndrome (HS) is the most severe form of DHTR in patients with SCD, in which both transfused and native red blood cell...
journal_title:Transfusion
pub_type:
doi:10.1111/trf.16003
更新日期:2020-08-26 00:00:00
abstract:BACKGROUND:Human immunodeficiency virus type 1 (HIV-1) subtype O infections are not reliably detected by commonly used anti-HIV-1/2 screening assays. Therefore, anti-HIV-1/2 assays have been modified to increase their sensitivity in detecting antibodies to HIV-1 subtype O. STUDY DESIGN AND METHODS:Two new anti-HIV-1/2...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1997.37897424408.x
更新日期:1997-08-01 00:00:00
abstract:BACKGROUND:Red blood cell (RBC) alloantibodies to nonself antigens may develop after transfusion or pregnancy, leading to morbidity and mortality in the form of hemolytic transfusion reactions or hemolytic disease of the newborn. A better understanding of the mechanisms of RBC alloantibody induction, or strategies to m...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.12217
更新日期:2014-01-01 00:00:00
abstract:BACKGROUND:In cases of massive hemorrhage in the US military, improved outcomes have been reported with the use of warm, fresh whole blood transfusions. Cold-stored low-titer type O whole blood (LTOWB) has become the preferred product for resuscitation of severe bleeding in deployed surgical units. Reports of LTOWB use...
journal_title:Transfusion
pub_type:
doi:10.1111/trf.15492
更新日期:2019-10-01 00:00:00
abstract:BACKGROUND:Screening for human T-lymphotropic virus type I (HTLV-I) infection became systematic in 1989 in the French West Indies for blood from all donors and in France for blood from natives of endemic areas; in 1990, it was extended to blood from donors with at-risk sex partners and in July 1991 to blood from all do...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1994.34394196615.x
更新日期:1994-03-01 00:00:00
abstract::Simple methods have been developed for adding and removing glycerol during freeze-preservation with 20 per cent W/V glycerol at minus 150 C, or with 40 per cent W/V glycerol at minus 80 C. A one-step method with a 35 per cent W/V glycerol solution is used to prepare 20 per cent W/V glycerolized red blood cells, and a ...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1975.15375160354.x
更新日期:1975-05-01 00:00:00
abstract::Ultraviolet B (UVB) irradiation of platelet concentrates (PCs) may prevent the development of posttransfusion HLA alloimmunization. As irradiation performed in a blood center or a hospital will probably be associated with a variable postirradiation delay before transfusion, the ability to store PCs after UVB irradiati...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1991.31691306243.x
更新日期:1991-07-01 00:00:00