Abstract:
BACKGROUND:Human immunodeficiency virus type 1 (HIV-1) subtype O infections are not reliably detected by commonly used anti-HIV-1/2 screening assays. Therefore, anti-HIV-1/2 assays have been modified to increase their sensitivity in detecting antibodies to HIV-1 subtype O. STUDY DESIGN AND METHODS:Two new anti-HIV-1/2 enzyme-linked immunosorbent assays (ELISAs) (Abbott Plus and Ortho Enhanced) were compared with a currently used anti-HIV-1/2 ELISA (Abbott Recombinant) in various serum panels: 91 Western blot-confirmed anti-HIV-1-positive samples, 20 samples from Western blot-confirmed HIV-1-infected patients in log3 serial dilutions, and 1463 samples from consecutive, volunteer, nonremunerated blood donors. RESULTS:Among 91 anti-HIV-1 Western blot-positive samples, 2 (2.2%) were missed by the Abbott Recombinant ELISA, but all 91 were detected by the Abbott Plus and Ortho Enhanced ELISAs. In contrast, two discrepant samples were found to react in viral lysate-based assays. In serial dilutions, Ortho Enhanced ELISA was significantly less sensitive than the Abbott Recombinant and Abbott Plus ELISAs, with the latter two being of comparable sensitivity. The specificities of Abbott Recombinant, Abbott Plus, and Ortho Enhanced ELISAs in 1463 blood donors were 100, 99.93, and 99.86 percent, respectively. Routine testing of 29,102 donations with the enhanced Abbott Plus ELISA revealed a specificity of 99.93 percent. CONCLUSION:Two Western blot-confirmed anti-HIV-1-positive samples were missed by the Abbott Recombinant ELISA but detected by the Abbott Plus and Ortho Enhanced ELISAs. The analytic sensitivity of the Ortho Enhanced ELISA was inferior to that of both Abbott ELISAs. The specificities of the Abbott Recombinant, Abbott Plus, and Ortho Enhanced ELISAs were comparable.
journal_name
Transfusionjournal_title
Transfusionauthors
Jongerius JM,van der Poel CL,van Loon AM,van den Akker R,Schaasberg W,van Leeuwen EFdoi
10.1046/j.1537-2995.1997.37897424408.xsubject
Has Abstractpub_date
1997-08-01 00:00:00pages
841-4issue
8eissn
0041-1132issn
1537-2995journal_volume
37pub_type
杂志文章相关文献
TRANSFUSION文献大全abstract:BACKGROUND:Loss of blood group ABO antigens on red blood cells (RBCs) is well known in patients with leukemias, and such decreased ABO expression has been reported to be strongly associated with hypermethylation of the ABO promoter. We investigated the underlying mechanism responsible for A-antigen reduction on RBCs in...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.15628
更新日期:2020-01-01 00:00:00
abstract:BACKGROUND:Fetal and neonatal alloimmune thrombocytopenia (FNAIT) is caused by maternal alloantibodies against fetal human platelet antigens (HPAs), mostly caused by anti-HPA-1a. Population-based screening for FNAIT is still a topic of debate. Logistically and financially, the major challenge for implementation is the ...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.15454
更新日期:2019-09-01 00:00:00
abstract:BACKGROUND:The ABO blood group is a major determinant in living donor kidney transplantation since AB antigens are expressed on renal tissue. Little attention has been directed to the ABH-secretor status of the donor kidney. As renal tissue is capable of secreting soluble ABH antigens in secretors, we examined the infl...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.13711
更新日期:2016-09-01 00:00:00
abstract:BACKGROUND:Improving apheresis technology may lead to an efficient and safe peripheral blood stem cell (PBSC) collection. Recently, the Spectra Optia (Optia, Terumo BCT) was introduced as an automated apheresis instrument, but comparisons with other instruments have been few. This is the first randomized multicenter an...
journal_title:Transfusion
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1111/trf.13777
更新日期:2016-11-01 00:00:00
abstract:BACKGROUND:We present an analysis of the first 2 years of hepatitis B virus (HBV) nucleic acid testing (NAT) of the Australian donor population. STUDY DESIGN AND METHODS:Between July 5, 2010, and July 4, 2012, all blood donations were screened for HBV DNA and hepatitis B surface antigen (HBsAg). Donors who tested HBsA...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.12556
更新日期:2014-08-01 00:00:00
abstract:BACKGROUND:Eleven laboratories participated in a study investigating whether swirling can be used in monitoring platelet discoid morphology in platelet concentrates (PCs). STUDY DESIGN AND METHODS:In each laboratory, two readers observed the swirling pattern in 1- to 5-day-old PCs, in PCs containing spherical platelet...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1994.34994378282.x
更新日期:1994-09-01 00:00:00
abstract::ADAMTS13 is an enzyme that acts by cleaving prothrombotic von Willebrand factor (VWF) multimers from the vasculature in a highly regulated manner. In pathologic states such as thrombotic thrombocytopenic purpura (TTP) and other thrombotic microangiopathies (TMAs), VWF can bind to the endothelium and form large multime...
journal_title:Transfusion
pub_type: 杂志文章,评审
doi:10.1111/trf.14804
更新日期:2018-10-01 00:00:00
abstract:BACKGROUND:Platelets (PLTs) are especially affected by the risk of bacterial contamination. Matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) is an accurate method for the routine identification of bacterial isolates in microbiology laboratories. We directly applied the MALDI-TOF method to bacteria...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.14430
更新日期:2018-03-01 00:00:00
abstract:BACKGROUND:Red blood cell (RBC) product wastage in hospitals is reported to range from 0.1% to 6.7%. Wastage at our institution averaged 4.4% of 63,000 issued RBC products. Data indicated that approximately 87% of wasted RBC units were either individual units that were out of blood bank for more than 30 minutes (dispen...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2010.02679.x
更新日期:2010-09-01 00:00:00
abstract::It has previously been shown that buffy coat platelet concentrates (BC-PCs) stored in a medium made up of approximately 70 percent platelet storage medium (Plasma-Lyte A, PL) and 30 percent plasma (BC-PC-P) are effective in vivo after 9 to 12 days of storage. In addition to sodium, potassium, magnesium, and chloride, ...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1992.32292180145.x
更新日期:1992-02-01 00:00:00
abstract:BACKGROUND:Cardiothoracic surgery places significant demands on blood bank resources. Measures aimed at reducing intraoperative hemodilution were initiated as part of a blood conservation program. STUDY DESIGN AND METHODS:We initiated a series of measures aimed at reducing hemodilution volume: 1) reduction of intraven...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.12745
更新日期:2014-10-01 00:00:00
abstract:BACKGROUND:In 5 percent of paternity determination cases, only DNA samples from the alleged father and child pairs are tested. The absence of the mother's DNA increases the probability of false paternity inclusions, which affects laboratories that use a limited number of DNA markers. The effect of coincidental matches ...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.2002.00152.x
更新日期:2002-07-01 00:00:00
abstract::Alloagglutinins of M and N specificites with unique characteristics found in normal sera are described. Specific M or N agglutination by these sera occurred only when erythrocytes had been exposed to glucose-containing solutions. Conversion to the form of MN recognized by these sera required incubation of the cells in...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1981.21682085751.x
更新日期:1981-11-01 00:00:00
abstract:BACKGROUND:TRALI is a serious adverse effect of blood transfusion. There is evidence that the condition is underrecognized and underreported. STUDY DESIGN AND METHODS:This study was an observational study carried out in a single hospital. RESULTS:Eleven cases of TRALI were recognized over 12 years. In 10 cases the im...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.2003.00466.x
更新日期:2003-08-01 00:00:00
abstract:BACKGROUND:Reports of Monosomy 7 in patients receiving granulocyte-colony-stimulating factor (G-CSF) have raised concerns that this cytokine may promote genomic instability. However, there are no studies addressing whether repeated administration of G-CSF produces Monosomy 7 aneuploidy in healthy donors. STUDY DESIGN ...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2011.03313.x
更新日期:2012-03-01 00:00:00
abstract:BACKGROUND:Sensitive and accurate counting methods are required to assess the residual white cells (WBCs) in WBC-reduced blood components. The Nageotte hemocytometer, widely used for this purpose, is cumbersome, and its efficacy is dependent upon the skill of the operator. The performance of a simple fluorochromatic as...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1997.37697335154.x
更新日期:1997-06-01 00:00:00
abstract:BACKGROUND:The hemoglobin-based oxygen carrier (HBOC-201) resuscitation fluid improves outcome in hemorrhagic shock swine models with minimal coagulopathy. Herein, coagulation parameters were evaluated after resuscitation with HBOC-201 after severe bleeding and prolonged delay to definitive care. STUDY DESIGN AND METH...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2007.01435.x
更新日期:2007-11-01 00:00:00
abstract:BACKGROUND:This study reports the incidence of Wr(a) antigen and anti-Wr(a) in Valencia, Spain. STUDY DESIGN AND METHODS:The incidence of the Wr(a) antigen in 110,000 healthy blood donors was estimated. Likewise, the incidence of anti-Wr(a) was analyzed in a population consisting of 730 healthy blood donors, 356 pregn...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2005.00196.x
更新日期:2005-08-01 00:00:00
abstract::Two blood samples containing autoagglutinins reactive in room temperature tests using low-ionic strength salt (LISS) reagents, but not by conventional saline-albumin or enzyme tests, are reported. The anti-P specificity of both sera, when tested near or diluted in group AB serum, was evident by their failure to agglut...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1982.22382224937.x
更新日期:1982-05-01 00:00:00
abstract:BACKGROUND:Recently, long-standing elevations of soluble growth factors released from platelets (PLTs) after contact with artificial surfaces during dialysis were described. They could be jointly responsible for the high frequency of death from cardiovascular diseases in dialysis patients. There are no comparable data ...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2005.04269.x
更新日期:2005-03-01 00:00:00
abstract:BACKGROUND:Dengue virus transmission by blood transfusion is a rarely reported event. CASE REPORT:During a dengue outbreak in São Paulo city, a regular plateletpheresis donor informed the blood bank of being diagnosed a few days after donation. The recipient was hospitalized and displayed symptoms and laboratory evide...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.12944
更新日期:2015-05-01 00:00:00
abstract::Female blood donors with low hematocrit levels detected by copper sulfate screening were selected randomly to receive either 75 mg of iron per day, as ferrous gluconate, or a calcium phosphate placebo. Their ferritin, serum iron, total iron-binding capacity, zinc protoporphyrin, and hemoglobin values, as well as their...
journal_title:Transfusion
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1046/j.1537-2995.1988.28588337328.x
更新日期:1988-09-01 00:00:00
abstract:BACKGROUND:Techniques for banking cord blood units (CBUs) as source for hematopoietic stem cell transplantation have been developed over the past 20 years, aimed to improve laboratory efficiency without altering the biologic properties of the graft. A large-scale, registry-based assessment of the impact of the banking ...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.13661
更新日期:2016-08-01 00:00:00
abstract:BACKGROUND:Whole blood donors may experience post-donation symptoms such as fatigue, dizziness, or headache after blood donation, which could influence donor retention. We aimed to examine post-donation symptoms during 1 week after whole blood donation, investigate donor characteristics associated with symptoms, and ev...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.16220
更新日期:2021-01-23 00:00:00
abstract:BACKGROUND:To investigate how a delay between pretransfusion platelet count measurement and actual platelet transfusion affects the assessment of platelet transfusion responses, the rate of reduction in platelet counts was determined in 30 patients with relatively uncomplicated thrombocytopenia. STUDY DESIGN AND METHO...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1997.37111298088046.x
更新日期:1997-11-01 00:00:00
abstract:BACKGROUND:A system has been developed to inactivate a wide spectrum of blood-borne pathogens in red blood cells (RBCs) before transfusion. The system utilizes S-303 to target nucleic acids of pathogens and white blood cells. The safety of pathogen inactivated RBC was assessed using S-303-treated RBCs (S-303 RBCs) and ...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2011.03132.x
更新日期:2011-10-01 00:00:00
abstract::The platelets used in this study were collected by serial centrifugation, and within four hours of collection were frozen with 5% dimethylsulfoxide (DMSO) at an overall rate of 2 to 3 C per minute by storage in a mechanical refrigerator at -80 C. The frozen platelets were stored for four to ten weeks before thawing an...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1979.19479250173.x
更新日期:1979-07-01 00:00:00
abstract:BACKGROUND:This study investigated the effects of a 6-month deferral due to low hemoglobin (Hb) on the subsequent donation patterns of Australian whole blood donors. STUDY DESIGN AND METHODS:The study was a retrospective cohort study of the donation patterns of all whole blood donors deferred for low Hb during a 2-mon...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2010.02881.x
更新日期:2011-03-01 00:00:00
abstract::Anti-Ytb was readily eluted from the cord red blood cells of the second child of a mother with both anti-Ytb and anti-Kell in her serum. The baby was Yt (a+b+) and Kell negative. The cord red blood cells were found to have a weakly positive direct antiglobulin test. Clinically evident hemolytic disease of the newborn ...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1979.19580059814.x
更新日期:1979-09-01 00:00:00
abstract:BACKGROUND:A previous study showed that white cells in blood units undergo apoptosis during storage. STUDY DESIGN AND METHODS:The present study attempts to show the release of nuclear matrix protein (NMP) in the supernatants of red cell units and to determine whether antibodies against nuclear components may be presen...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.2000.40020169.x
更新日期:2000-02-01 00:00:00