Nuclear matrix protein is released from apoptotic white cells during cold (1-6 degrees C) storage of concentrated red cell units and might induce antibody response in multiply transfused patients.

Abstract:

BACKGROUND:A previous study showed that white cells in blood units undergo apoptosis during storage. STUDY DESIGN AND METHODS:The present study attempts to show the release of nuclear matrix protein (NMP) in the supernatants of red cell units and to determine whether antibodies against nuclear components may be present in multiply transfused patients; the methods employed were enzyme-linked immunosorbent assay, flow cytometry, microscopy, immunoblotting, immunofluorescence, and confocal laser-scanning microscopy. RESULTS:NMP is released from white cells in the supernatant of packed red cell units upon cold storage (1-6 degrees C). The concentration of NMP correlates well with the degree of apoptosis, as analyzed by flow cytometry, nuclear dye staining, and DNA gel electrophoresis. Immunofluorescence also shows that white cells undergoing apoptosis (pre-G(1) peak, as seen by propidium iodide staining and flow cytometry) have an NMP content lower than control cells, which confirms an actual release of NMP. Moreover, immunoblotting analysis and immunofluorescent staining showed that, in 4 of 38 multiply transfused patients, autoantibodies against NMPs were present without any clinical or laboratory sign of autoimmune disease. One of the sera, recognizing a 64-kDa NMP, immunostained nuclear dots that were identified as coiled bodies because of their colocalization with p 80 coilin. CONCLUSION:NMP is released in the supernatant of red cell units. The results obtained from patients suggest that nuclear proteins released during apoptosis, once transfused, may induce an immune response in multiply transfused patients.

journal_name

Transfusion

journal_title

Transfusion

authors

Martelli AM,Tazzari PL,Bortul R,Riccio M,Tabellini G,Santi S,Frabetti F,Musiani D,Bareggi R,Conte R

doi

10.1046/j.1537-2995.2000.40020169.x

subject

Has Abstract

pub_date

2000-02-01 00:00:00

pages

169-77

issue

2

eissn

0041-1132

issn

1537-2995

journal_volume

40

pub_type

杂志文章
  • Storage of aliquots of apheresis platelets for neonatal use in syringes with and without agitation.

    abstract:BACKGROUND:To facilitate volume control in neonates, platelets (PLTs) are aliquoted and stored for short periods in non-gas-permeable syringes before infusion. Although agitation of PLTs during storage in gas-permeable bags is performed to maintain their quality, the effect of syringe agitation during storage is unknow...

    journal_title:Transfusion

    pub_type: 临床试验,杂志文章

    doi:10.1111/j.1537-2995.2011.03230.x

    authors: Diab Y,Wong E,Criss VR,Moroff G,Wagner SJ,Luban NL

    更新日期:2011-12-01 00:00:00

  • Blood use in the ambulatory setting among elderly in the United States.

    abstract:BACKGROUND:Our study characterizes blood use in the ambulatory setting by US elderly Medicare beneficiaries during 2001. As the US population ages and delivery of health care in outpatient settings is on the rise, ambulatory blood utilization is expected to increase. There is currently a lack of broad population-based ...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/j.1537-2995.2009.02114.x

    authors: Menis M,Burwen DR,Holness L,Anderson SA

    更新日期:2009-06-01 00:00:00

  • Autologous blood donation: hemodynamics in a high-risk patient population.

    abstract::Transfusion practices have changed dramatically in recent years as a result of the acquired immune deficiency syndrome crisis. The use of preoperative donation of autologous blood units has gained popularity. Healthy individuals tolerate phlebotomy well, experiencing a 2- to 5-percent incidence of vasovagal reactions....

    journal_title:Transfusion

    pub_type: 评论,杂志文章

    doi:10.1046/j.1537-2995.1992.32192116425.x

    authors: Spiess BD,Sassetti R,McCarthy RJ,Narbone RF,Tuman KJ,Ivankovich AD

    更新日期:1992-01-01 00:00:00

  • Trend in ABO-incompatible RBC transfusion-related fatalities reported to the FDA, 2000-2019.

    abstract:BACKGROUND:ABO-incompatible red blood cell (RBC) transfusions and acute hemolytic reactions occur infrequently, yet resultant fatalities are reported to the US Food and Drug Administration (FDA) every year. We describe a 20-year retrospective study of reported mistransfusion cases to identify temporal trends, common ca...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/trf.16121

    authors: Storch EK,Rogerson B,Eder AF

    更新日期:2020-12-01 00:00:00

  • Evaluation of combined idarucizumab and prothrombin complex concentrate treatment for bleeding related to dabigatran in a lethal porcine model of double trauma.

    abstract:BACKGROUND:Idarucizumab (IDA) is approved for emergency reversal of dabigatran; prothrombin complex concentrates (PCCs) are recommended in the absence of specific antidote. The combined effects of IDA and PCC in trauma-related bleeding are unknown. The efficacy and safety of combined IDA + PCC were assessed in a lethal...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/trf.15117

    authors: Honickel M,Braunschweig T,Rossaint R,Schöchl H,Grottke O

    更新日期:2019-04-01 00:00:00

  • Frequency of glucose-6-phosphate dehydrogenase-deficient red blood cell units in a metropolitan transfusion service.

    abstract:BACKGROUND:Glucose-6-phosphate dehydrogenase (G6PD) deficiency is characterized by red blood cell (RBC) destruction in response to oxidative stress. Although blood donors are not routinely screened for G6PD deficiency, the transfusion of stored G6PD-deficient RBCs may have serious adverse outcomes. By measuring G6PD en...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/j.1537-2995.2012.03765.x

    authors: Francis RO,Jhang J,Hendrickson JE,Zimring JC,Hod EA,Spitalnik SL

    更新日期:2013-03-01 00:00:00

  • A "new" low-incidence red cell antigen, LOCR, associated with altered expression of Rh antigens.

    abstract:BACKGROUND:Mild cases of hemolytic disease of the newborn were being studied when it was recognized that the propositi's red cells carried a novel antigen. STUDY DESIGN AND METHODS:Serologic and genetic studies of a new low-incidence antigen, LOCR (International Society of Blood Transfusion series number 700.53), were...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1994.34694295063.x

    authors: Coghlan G,McCreary J,Underwood V,Zelinski T

    更新日期:1994-06-01 00:00:00

  • A multicenter evaluation of reproducibility of swirling in platelet concentrates. Biomedical Excellence for Safer Transfusion (BEST) Working Party of the International Society of Blood Transfusion.

    abstract:BACKGROUND:Eleven laboratories participated in a study investigating whether swirling can be used in monitoring platelet discoid morphology in platelet concentrates (PCs). STUDY DESIGN AND METHODS:In each laboratory, two readers observed the swirling pattern in 1- to 5-day-old PCs, in PCs containing spherical platelet...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1994.34994378282.x

    authors: Bertolini F,Murphy S

    更新日期:1994-09-01 00:00:00

  • Importance of ionized magnesium measurement for monitoring of citrate-anticoagulated plateletpheresis.

    abstract:BACKGROUND:The infusion of citrate during apheresis may affect the levels of ionized magnesium in the blood. Hypomagnesemia and concomitant hypocalcemia could influence the parathormone response and could be responsible for some of the symptoms observed during apheresis. STUDY DESIGN AND METHODS:The study reports meas...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1997.37497265343.x

    authors: Mercan D,Bastin G,Lambermont M,Dupont E

    更新日期:1997-04-01 00:00:00

  • Cellular therapy services provided by blood centers and hospitals in the United States, 1999: an analysis from the Nationwide Blood Collection and Utilization Survey.

    abstract:BACKGROUND:The 2000 Nationwide Blood Collection and Utilization Survey was designed to assess cellular therapy product services in US blood centers and hospitals. STUDY DESIGN AND METHODS:Questionnaires were returned by 2,040 institutions. Data were analyzed for 30 quantitative variables related to cellular therapy pr...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/j.1537-2995.2003.03286.x

    authors: Read EJ,Sullivan MT

    更新日期:2004-04-01 00:00:00

  • Evaluation and comparison of coagulation factor activity in fresh-frozen plasma and 24-hour plasma at thaw and after 120 hours of 1 to 6°C storage.

    abstract:BACKGROUND:Current transfusion-related acute lung injury reduction strategies include avoiding transfusion of plasma products collected from female donors or female donors that have been pregnant to reduce transfusion of plasma-containing HLA antibodies. Such a policy considerably decreases the number of donors availab...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/j.1537-2995.2009.02198.x

    authors: Scott E,Puca K,Heraly J,Gottschall J,Friedman K

    更新日期:2009-08-01 00:00:00

  • Abnormal electrocardiographic findings in apheresis donors.

    abstract::Electrocardiographic (ECG) monitoring was performed on 291 donors during apheresis. Twenty-one donors (7.2%) had clinical symptoms such as discomfort, nausea, chill, numbness, and paresthesia, and 13 of this group exhibited ECG abnormalities, such as tachycardia, bradycardia, and other abnormal wave patterns. The dono...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1988.28488265270.x

    authors: Komatsu F,Shikata M

    更新日期:1988-07-01 00:00:00

  • Cytokines and clinical predictors in distinguishing pulmonary transfusion reactions.

    abstract:BACKGROUND:Pulmonary transfusion reactions are important complications of blood transfusion, yet differentiating these clinical syndromes is diagnostically challenging. We hypothesized that biologic markers of inflammation could be used in conjunction with clinical predictors to distinguish transfusion-related acute lu...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/trf.13021

    authors: Roubinian NH,Looney MR,Kor DJ,Lowell CA,Gajic O,Hubmayr RD,Gropper MA,Koenigsberg M,Wilson GA,Matthay MA,Toy P,Murphy EL,TRALI Study Group.

    更新日期:2015-08-01 00:00:00

  • Toward extended phenotype matching: a new operational paradigm for the transfusion service.

    abstract:BACKGROUND:Conventional pretransfusion testing uses hemagglutination to ensure donor-recipient compatibility for ABO/D status and recipient alloantibodies. While screening large numbers of donor units for multiple antigens by hemagglutination is impractical, novel methods of DNA analysis permit the rapid determination ...

    journal_title:Transfusion

    pub_type: 杂志文章,多中心研究

    doi:10.1111/j.1537-2995.2009.02462.x

    authors: Klapper E,Zhang Y,Figueroa P,Ness P,Stubbs J,Abumuhor I,Bailey J,Epperson L,Tauscher C,Enriquez E,Hashmi G,Seul M

    更新日期:2010-03-01 00:00:00

  • Cases of transfusion-transmitted babesiosis occurring in nonendemic areas: a diagnostic dilemma.

    abstract:BACKGROUND:Transfusion-transmitted babesiosis (TTB) has been rapidly increasing in incidence since the beginning of the 21st century. Asymptomatic individuals with Babesia infection are able to donate blood in the United States because of the lack of specific blood donation testing. Blood products collected in Babesia-...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/trf.14246

    authors: LeBel DP 2nd,Moritz ED,O'Brien JJ,Lazarchick J,Tormos LM,Duong A,Fontaine MJ,Squires JE,Stramer SL

    更新日期:2017-10-01 00:00:00

  • Development of a real-time polymerase chain reaction assay for sensitive detection and quantitation of Babesia microti infection.

    abstract:BACKGROUND:Babesia microti, the most frequently implicated pathogen in transfusion-transmitted babesiosis, is widely endemic in the Northeast and upper Midwestern United States. High seroprevalence in endemic areas limits antibody-based donor screening. A high-performance molecular test is needed to identify donors in ...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/trf.12098

    authors: Bloch EM,Lee TH,Krause PJ,Telford SR 3rd,Montalvo L,Chafets D,Usmani-Brown S,Lepore TJ,Busch MP

    更新日期:2013-10-01 00:00:00

  • The antiglobulin reaction with native and enzyme-modified red cells.

    abstract::The quantity of anti-IgG bound at equilibrium to cell-bound anti-D failed to increase with enzymatic modification of intact red blood cells. This contrasts with results obtained by immunoelectronmicroscopy with unsealed red blood cell ghosts. Ghosts derived from enzyme-modified, anti-D-sensitized intact red blood cell...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1980.20480260268.x

    authors: Rearden A,Masouredis SP

    更新日期:1980-07-01 00:00:00

  • Probability of receiving testing in a national lookback program: the English HCV experience.

    abstract:BACKGROUND:The HCV lookback program was designed to trace and offer testing to recipients who received transfusion of blood components from donors subsequently found to be anti-HCV positive. Only approximately 20 percent of transfusable components entering lookback did result in a recipient obtaining testing through th...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.2002.00171.x

    authors:

    更新日期:2002-09-01 00:00:00

  • Collection and transfusion of blood and blood components in the United States, 1994.

    abstract:BACKGROUND:Collections and transfusions of blood in the United States in 1994 were measured and compared with those in 1992. STUDY DESIGN AND METHODS:Completed survey questionnaires were returned by all 147 regional blood centers, 1340 American Association of Blood Banks (AABB) member hospitals, and 523 non-AABB hospi...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1998.38798346630.x

    authors: Wallace EL,Churchill WH,Surgenor DM,Cho GS,McGurk S

    更新日期:1998-07-01 00:00:00

  • Systematic review of rituximab for autoimmune diseases: a potential alternative to intravenous immune globulin.

    abstract:BACKGROUND:The anti-CD20 monoclonal antibody rituximab has immune-modulatory effects similar to intravenous immunoglobulin (IVIG). We performed a systematic review and meta-analysis to determine the efficacy and safety of rituximab in autoimmune diseases that are also treated with IVIG. STUDY DESIGN AND METHODS:The mo...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/trf.14841

    authors: MacIsaac J,Siddiqui R,Jamula E,Li N,Baker S,Webert KE,Evanovitch D,Heddle NM,Arnold DM

    更新日期:2018-11-01 00:00:00

  • Semiautomated data analysis of flow cytometric estimation of fetomaternal hemorrhage in D- women.

    abstract:BACKGROUND:Accurate and reliable measurement of the volume of fetal D+ cells in D- women is required for adequate anti-D prophylaxis. A semiautomated flow cytometry assay based on a standardized calibration curve that was created with simulated fetomatemal hemorrhage (FMH) mixtures was developed. STUDY DESIGN AND METH...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.2002.00159.x

    authors: Greiss MA,Armstrong-Isher SS,Perera WS,Brown PM,Urbaniak SJ

    更新日期:2002-08-01 00:00:00

  • Red blood cell minor antigen mismatches during chronic transfusion therapy for sickle cell anemia.

    abstract:BACKGROUND:Red blood cell (RBC) alloimmunization occurs at a high frequency in sickle cell anemia (SCA) despite serologic matching for Rh (C/c, E/e) and K antigens. RBC minor antigen genotyping allows for prediction of antigens and RH variants that may lead to alloimmunization. STUDY DESIGN AND METHODS:RBC antigen gen...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/trf.14282

    authors: Yee MEM,Josephson CD,Winkler AM,Webb J,Luban NLC,Leong T,Stowell SR,Fasano RM

    更新日期:2017-11-01 00:00:00

  • Retrograde transmission of Proteus mirabilis during platelet transfusion and the use of arbitrarily primed polymerase chain reaction for bacteria typing in suspected cases of transfusion transmission of infection.

    abstract:BACKGROUND:When bacteria are found, after a platelet transfusion, in the recipient's blood as well as in the platelet concentrate (PC), a causal relationship is normally suspected, with the PC as the causative agent. The other alternative, that the patient has bacteremia and contaminated the PC, is less well documented...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1995.351096026371.x

    authors: Engstrand M,Engstrand L,Högman CF,Hambraeus A,Branth S

    更新日期:1995-10-01 00:00:00

  • Plasma ectonucleotidases prevent desensitization of purinergic receptors in stored platelets: importance for platelet activity during thrombus formation.

    abstract:BACKGROUND:Platelets (PLTs) contain purinergic receptors for ATP (P2X1) and ADP (P2Y1 and P2Y12) that rapidly desensitize upon stimulation with these nucleotides. In vivo, this is antagonized by ectonucleotidases on the surface of endothelial cells and white blood cells (WBCs). The receptor desensitization of ATP- and ...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/j.1537-2995.2006.00837.x

    authors: Cauwenberghs S,Feijge MA,Hageman G,Hoylaerts M,Akkerman JW,Curvers J,Heemskerk JW

    更新日期:2006-06-01 00:00:00

  • A general method for concentrating blood samples in preparation for counting very low numbers of white cells.

    abstract:BACKGROUND:To count extremely low levels of white cells (WBCs) in WBC-reduced blood components, a larger volume of sample must be processed. The goal was to develop an all-purpose method for concentrating the samples obtained from WBC-reduced red cells or platelets. The method was designed to be compatible with a varie...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1997.37397240209.x

    authors: Szuflad P,Dzik WH

    更新日期:1997-03-01 00:00:00

  • Computerized selection of donors for inclusion in red cell antibody panels.

    abstract::This study evaluated various algorithms for the generation of a red cell antibody identification panel by computer. The algorithms depended on schemes that optimized Fisher's exact p value for each specificity in the panel rather than using predetermined formulations of desired phenotypic combinations. Panels produced...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1987.27187121480.x

    authors: AuBuchon JP,Roberts GC

    更新日期:1987-01-01 00:00:00

  • Sensitivity of HCV RNA and HIV RNA blood screening assays.

    abstract:BACKGROUND:The FDA requirement for sensitivity of viral NAT methods used in blood screening is a 95-percent detection limit of 100 copies per mL, whereas the NAT screening system should have a sensitivity of at least 5000 copies per mL per individual donation. According to the Common Technical Specifications of the Eur...

    journal_title:Transfusion

    pub_type: 杂志文章,多中心研究

    doi:10.1046/j.1537-2995.2002.00101.x

    authors: Lelie PN,van Drimmelen HA,Cuypers HT,Best SJ,Stramer SL,Hyland C,Allain JP,Moncharmont P,Defer C,Nübling M,Glauser A,da Silva Cardoso M,Viret JF,Lankinen MH,Grillner L,Wirthmüller U,Coste J,Schottstedt V,Masecar B,D

    更新日期:2002-05-01 00:00:00

  • Functional antibodies to Haemophilus influenzae type B, Neisseria meningitidis, and Streptococcus pneumoniae contained in intravenous immunoglobulin products.

    abstract:BACKGROUND:Intravenous immunoglobulin G (IVIG) replacement therapy is used to prevent invasive infections in patients with primary antibody deficiency (PAD). However, few studies have functionally evaluated specific antibodies against encapsulated bacteria that cause invasive infection in patients with PAD. In this stu...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/trf.13869

    authors: Lee S,Kim HW,Kim KH

    更新日期:2017-01-01 00:00:00

  • Circulating red cells usually remain of host origin after bone marrow transplantation for severe combined immunodeficiency.

    abstract:BACKGROUND:Patients with severe combined immunodeficiency (SCID) treated with allogeneic bone marrow transplantation often receive a milder conditioning regimen than patients who undergo transplantation for hematologic malignancy, and they regularly retain circulating white cells of host origin. The origin of circulati...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1996.36496226143.x

    authors: Brady KA,Cowan MJ,Leavitt AD

    更新日期:1996-04-01 00:00:00

  • Freeze-dried plasma stability under prehospital field conditions.

    abstract:BACKGROUND:This study evaluated the effect of routine, uncontrolled, Israeli field storage conditions on the stability and efficacy of Lyo-Plas N freeze-dried plasma (FDP). We evaluated clotting factors V, VIII, and XI; proteins S and C; fibrinogen; partial thromboplastin time (PTT); antithrombin III (ATIII); von Wille...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/trf.15533

    authors: Zur M,Glassberg E,Gorenbein P,Epstein E,Eisenkraft A,Misgav M,Avramovich E

    更新日期:2019-11-01 00:00:00