Abstract:
BACKGROUND:Platelets (PLTs) are especially affected by the risk of bacterial contamination. Matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) is an accurate method for the routine identification of bacterial isolates in microbiology laboratories. We directly applied the MALDI-TOF method to bacterial detection in PLTs. In this study, we evaluated the sensitivity, specificity, and speed of a direct MALDI-TOF approach compared to the conventional method BACTEC. STUDY DESIGN AND METHODS:Eight bacteria associated with PLT contamination, cited by the ISBT on transfusion-transmitted infectious diseases, were spiked into PLTs for a final concentration of approximately 100 CFU/bag (n = 5 for each strain). The PLTs were then agitated for 24 hours. One milliliter of PLTs was incubated in a shaker incubator for 8 hours at 37°C with 1 mL of trypticase soy broth (TSB). The spectra were analyzed using the MALDI Biotyper software. As a control, 8 mL of PLTs incubated into BACTEC bottles and a positive bottle were subcultured to ensure identification of bacterial growth. RESULTS:Regardless of the strain of PLTs tested, MALDI-TOF analysis made detection and early identification possible at 8 hours. Analysis by BACTEC of PLTs infected with Escherichia coli, Bacillus cereus, and Providencia stuartii made early identification possible. For the remaining bacteria, the detection time by BACTEC was significantly longer than 8 hours. CONCLUSION:We demonstrated the possibility of detecting bacteria in PLTs using a standardized culture step in TSB with MALDI-TOF, regardless of the strain, with the same specificity and analytical sensitivity and with a time to results of 12 hours. This direct method presented rapid and reliable results.
journal_name
Transfusionjournal_title
Transfusionauthors
Chetouane Y,Dubourg G,Gallian P,Flaudrops C,Chiaroni J,Chabrière E,Raoult D,Camoin-Jau Ldoi
10.1111/trf.14430subject
Has Abstractpub_date
2018-03-01 00:00:00pages
766-773issue
3eissn
0041-1132issn
1537-2995journal_volume
58pub_type
杂志文章相关文献
TRANSFUSION文献大全abstract:BACKGROUND:Transfusion medicine lacks a standard method for the systematic collection and analysis of event reports. Review of event reports from the Food and Drug Administration (FDA) showed a relative paucity of information on event causation. Thus, a causal analysis method was developed as part of a prototype Medica...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1998.38111299056319.x
更新日期:1998-11-01 00:00:00
abstract:BACKGROUND:Antibodies to Mia , MUT, and Mur are among the most frequently identified alloantibodies in Southeast Asia. Understanding the characteristics of these antibodies in terms of induction and evanescence would aid in optimizing methods for their detection. STUDY DESIGN AND METHODS:Antibody testing results betwe...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.14538
更新日期:2018-05-01 00:00:00
abstract:BACKGROUND:Antigens of the human blood group system Gerbich (Ge) are located on sialoglycoproteins glycophorin C (GPC) and glycophorin D (GPD). CASE REPORT:The Ge+ proposita (RW) produced an alloanti-Ge after receiving 2 units of red cells (RBCs) during surgery. Further studies were carried out to characterize the ant...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1997.371098016440.x
更新日期:1997-10-01 00:00:00
abstract::Cell pheresis was employed in the initial management of a pregnant patient with chronic myelocytic leukemia (CML). Reduction in circulating leukocyte and platelet counts was evident after two weeks of therapy. This technique is an effective mode of management of the pregnant patient with CML and may play an important ...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1978.18178118555.x
更新日期:1978-01-01 00:00:00
abstract::Red cells from patients with sickle cell disease (HbSS and HbSC) are more resistant to lysis in hypotonic NaCl solutions than normal (HbAA) red cells. Taking advantage of this inherent resistance to osmotic stress, patient red cells (HbSS or HbSC) were rapidly isolated from donor red cells (HbAA or HbAS) by washing wi...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1988.28188127945.x
更新日期:1988-01-01 00:00:00
abstract:BACKGROUND:Hemolysis at the time of graft infusion is one of the immediate complications in major ABO-incompatible allogeneic hematopoietic stem cell transplants (HSCTs). We conducted a retrospective analysis to evaluate the efficacy of donor-type fresh frozen plasma (FFP) in reducing isohemagglutinin titer and prevent...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.15053
更新日期:2019-01-01 00:00:00
abstract:BACKGROUND:The use of blood-saving techniques in elective surgery can produce a favorable cost-benefit ratio only when there is a reasonable likelihood that transfusion will be required. To apply a targeted blood-sparing technique in lung cancer surgery, the patient's preoperative characteristics that predict the use o...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.2000.40101228.x
更新日期:2000-10-01 00:00:00
abstract:BACKGROUND:Understanding the donor base, infectious disease prevalence, and donation loss at various blood donation sites will help maximize blood collection efforts and blood availability. STUDY DESIGN AND METHODS:Using donation data collected at five US blood centers, the prevalence of HIV, HTLV, HBsAg, and HCV in f...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.2003.00399.x
更新日期:2003-06-01 00:00:00
abstract::The immediate-spin (IS) crossmatch is a method that detects ABO incompatibility. However, under certain circumstances, this test may show unwanted negative or weak results, even though the red cells (RBCs) and serum being tested are ABO incompatible with each other. The present study investigated the potential effect ...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1991.31391165166.x
更新日期:1991-03-01 00:00:00
abstract:BACKGROUND:In 2014, we experienced the first isolation of Lactococcus garvieae from a platelet concentrate (PC). Thereafter, L. garvieae contamination of PCs occurred in two more cases in Japan. It is rare that bacterial contamination with uncommon strains like this species occurs frequently within a short period. Ther...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.15836
更新日期:2020-07-01 00:00:00
abstract:BACKGROUND:In sub-Saharan Africa (SSA) confirmed viral marker prevalence between family donors (FDs) and first-time volunteer nonremunerated donors (VNRDs) is similar. In a blood service collecting 10 units/1000 inhabitants, a questionnaire examined FD donation conditions and willingness of becoming repeat VNRDs. STUD...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.12216
更新日期:2014-03-01 00:00:00
abstract::Electrocardiographic (ECG) monitoring was performed on 291 donors during apheresis. Twenty-one donors (7.2%) had clinical symptoms such as discomfort, nausea, chill, numbness, and paresthesia, and 13 of this group exhibited ECG abnormalities, such as tachycardia, bradycardia, and other abnormal wave patterns. The dono...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1988.28488265270.x
更新日期:1988-07-01 00:00:00
abstract:BACKGROUND:On rare occasions, upon thawing of stored cryoprecipitate components, clots are observed on visual inspection. Although it has been assumed that the clot reflects fibrinogen to fibrin conversion, there are few published studies that document that this assumption is correct. Our studies were conducted to furt...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2012.03778.x
更新日期:2013-03-01 00:00:00
abstract:BACKGROUND:A quality monitoring program (QMP) for platelet concentrates (PCs) was implemented at Canadian Blood Services (CBS) to improve standards and to better understand platelet (PLT) products by supplementing routine quality control (QC). STUDY DESIGN AND METHODS:Annual surveys of PCs from CBS production sites we...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2011.03402.x
更新日期:2012-04-01 00:00:00
abstract::The ability of the monocyte monolayer assay (MMA) and the chemiluminescence test (CLT) to predict the clinical significance of alloantibodies associated with hemolytic disease of the newborn (HDN) was assessed by the use of 22 well-characterized antisera--predominantly anti-D--from alloimmunized pregnant women. Sevent...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1993.33693296810.x
更新日期:1993-06-01 00:00:00
abstract:BACKGROUND:All studied photosensitizers for virus inactivation impair RBCs. To reduce damage to the RBCs without affecting virucidal activity, selective protection of the RBCs is necessary. The ability of the band 3 ligand, dipyridamole, to react with singlet oxygen and to increase the selectivity of photosterilization...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.2000.40111330.x
更新日期:2000-11-01 00:00:00
abstract::Exchange transfusions using whole blood less than 24 hours old and deglycerolized red cells reconstituted with fresh-frozen plasma were performed in two premature infants for the treatment of hyperkalemia and cardiac arrhythmia. Improvement of their condition was immediate. ...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1985.25485273824.x
更新日期:1985-07-01 00:00:00
abstract:BACKGROUND:RhIG is obtained from hyperimmunized healthy anti-D donors (HIDs) boosted with D+ red blood cells (RBCs). One hypothesis for its mechanism of action is fast clearance of opsonized D+ RBCs through Fcγ receptor (FcγR)III. Levels of immunoglobulin (Ig)G Fc-fucosylation influence interactions with FcγRIII, with ...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.12880
更新日期:2015-03-01 00:00:00
abstract::Anti-D sensitized red cells were converted to stroma, either by digitonin or hypotonic lysis, and eluates prepared by the modified rapid acid-stromal method. SDS-PAGE and both rocket- and crossed-immunoelectrophoresis showed that, of the total protein content of the eluates, 11 to 25 percent was IgG, 18 to 45 percent ...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1983.23383224906.x
更新日期:1983-05-01 00:00:00
abstract:BACKGROUND:Neutrophil specific Fcγ receptor IIIb (CD16b) is a low-affinity IgG receptor. Its polymorphic variants are associated with human neutrophil antigens (HNA). HNA-1a and HNA-1b differ in four amino acids. Immunization can lead to the production of alloantibodies. The exact contribution of four amino acid exchan...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.15707
更新日期:2020-04-01 00:00:00
abstract::A prospective double-blind study compared a manual hexadimethrine bromide (Polybrene) antiglobulin antibody detection test (P-AHG) and crossmatch with the albumin-antiglobulin antibody detection test and saline-antiglobulin crossmatch routinely used in our laboratory. A total of 10,084 pretransfusion blood samples fro...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1987.27287150183.x
更新日期:1987-03-01 00:00:00
abstract:BACKGROUND:During the use of commercial red cell (RBC) acid-elution kits for adsorption and elution (adsorption/elution) studies with anti-D, unexpected reactive eluates (anti-D) were obtained from D- RBCs. Such results were not obtained with a parallel xylene method or, historically, with heat and ether methods. STUD...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1998.38698326336.x
更新日期:1998-06-01 00:00:00
abstract:BACKGROUND:Dengue virus transmission by blood transfusion is a rarely reported event. CASE REPORT:During a dengue outbreak in São Paulo city, a regular plateletpheresis donor informed the blood bank of being diagnosed a few days after donation. The recipient was hospitalized and displayed symptoms and laboratory evide...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.12944
更新日期:2015-05-01 00:00:00
abstract:BACKGROUND:The FcgammaRIIA gene is expressed in two polymorphic forms, R131 and H131, which differ by the replacement of histidine by arginine at position 131. The FCGR3B (FcgammaRIIIB) gene exists in two allelic isoforms, known as FCGR3B1 (FcgammaRIIIB-NA1) and FCGR3B2 (FcgammaRIIIB-NA2), which differ in nucleotides 1...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.2000.40111388.x
更新日期:2000-11-01 00:00:00
abstract::As an adjunct to antibiotic therapy, granulocytes for infusion often are required within hours of the diagnosis of neonatal sepsis. The logistics of obtaining granulocytes by leukapheresis were evaluated in order to provide, at short notice, a low volume, highly concentrated product. An average of 4.0 X 10(9) granuloc...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1984.24384225030.x
更新日期:1984-05-01 00:00:00
abstract:BACKGROUND:The Am phenotype has been characterized as a weak expression of the A antigen on red blood cells but the presence of a normal quantity of the A antigen in saliva. This study describes a molecular genetic analysis of members of an Am family. STUDY DESIGN AND METHODS:The eight exon regions of the ABO genes of...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2005.04132.x
更新日期:2005-01-01 00:00:00
abstract::Cryoprecipitate is used infrequently in home therapy for patients with hemophilia A since freezer storage is required and resuspension and pooling of thawed cryoprecipitate is cumbersome. We evaluated procedures for preparation of cryoprecipitate in an "open system" so that four to six bags of cryoprecipitate could be...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1984.24685066816.x
更新日期:1984-11-01 00:00:00
abstract:BACKGROUND:AABB Standard 5.27 requires transfusion services to have a process for urgent release of blood before completion of compatibility testing. Our institution endorses a policy for the emergency release of group O, D+ red blood cells (RBC; O+ RBC) to males and females at least 50 years of age. Our emergency depa...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.12925
更新日期:2015-04-01 00:00:00
abstract:BACKGROUND:Aprotinin has been reapproved for use in Europe and in Canada. We sought to determine if its reintroduction was still pertinent given the widespread administration of tranexamic acid, another antifibrinolytic shown to reduce bleeding and transfusions. STUDY DESIGN AND METHODS:After institutional review boar...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.13615
更新日期:2016-07-01 00:00:00
abstract::Donation willingness is generally high but registering with donor centers lags behind. We need to understand why and when during the life course which groups of individuals are motivated to donate. With this knowledge, we can design targeted recruitment and retention strategies and improve evidence-based donor managem...
journal_title:Transfusion
pub_type: 杂志文章,评审
doi:10.1111/trf.15268
更新日期:2019-06-01 00:00:00