Abstract:
:The intestinal microbiota is important for induction of inflammatory bowel disease (IBD). IBD is associated with complex shifts in microbiota composition, but it is unclear whether specific bacterial subsets induce IBD and, if so, whether their proportions in the microbiota are altered during disease. Here, we fulfilled Koch's postulates in host-genotype-specific fashion using a mouse model of IBD with human-relevant disease-susceptibility mutations. From screening experiments we isolated common commensal Bacteroides species, introduced them into antibiotic-pretreated mice, and quantitatively reisolated them in culture. The bacteria colonized IBD-susceptible and -nonsusceptible mice equivalently, but induced disease exclusively in susceptible animals. Conversely, commensal Enterobacteriaceae were >100-fold enriched during spontaneous disease, but an Enterobacteriaceae isolate failed to induce disease in antibiotic-pretreated mice despite robust colonization. We thus demonstrate that IBD-associated microbiota alterations do not necessarily reflect underlying disease etiology. These findings establish important experimental criteria and a conceptual framework for understanding microbial contributions to IBD.
journal_name
Cell Host Microbejournal_title
Cell host & microbeauthors
Bloom SM,Bijanki VN,Nava GM,Sun L,Malvin NP,Donermeyer DL,Dunne WM Jr,Allen PM,Stappenbeck TSdoi
10.1016/j.chom.2011.04.009subject
Has Abstractpub_date
2011-05-19 00:00:00pages
390-403issue
5eissn
1931-3128issn
1934-6069pii
S1931-3128(11)00131-4journal_volume
9pub_type
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