Abstract:
OBJECTIVES:Secondary bacterial pneumonia is common following influenza infection. However, it remains unclear about the underlying molecular mechanisms. MATERIALS AND METHODS:We established a mouse model of post-influenza S aureus pneumonia using conditional Shp2 knockout mice (LysMCre/+ :Shp2flox/flox ). The survival, bacterial clearance, pulmonary histology, phenotype of macrophages, and expression of type I interferons and chemokines were assessed between SHP2 deletion and control mice (Shp2flox/flox ). We infused additional KC and MIP-2 to examine the reconstitution of antibacterial immune response in LysMCre/+ :Shp2flox/flox mice. The effect of SHP2 on signal molecules including MAPKs (JNK, p38 and Erk1/2), NF-κB p65 and IRF3 was further detected. RESULTS:LysMCre/+ :Shp2flox/flox mice displayed impaired antibacterial immunity and high mortality compared with control mice in post-influenza S aureus pneumonia. The attenuated antibacterial ability was associated with the induction of type I interferon and suppression of chemo-attractants KC and MIP-2, which reduced the infiltration of neutrophils into the lung upon secondary bacterial invasion. In additional, Shp2 knockout mice displayed enhanced polarization to alternatively activated macrophages (M2 phenotype). Further in vitro analyses consistently demonstrated that SHP2-deficient macrophages were skewed towards an M2 phenotype and had a decreased antibacterial capacity. Moreover, SHP2 modulated the inflammatory response to secondary bacterial infection via interfering with NF-κB and IRF3 signalling in macrophages. CONCLUSIONS:Our findings reveal that the SHP2 expression enhances the host immune response and prompts bacterial clearance in post-influenza S aureus pneumonia.
journal_name
Cell Prolifjournal_title
Cell proliferationauthors
Ouyang W,Liu C,Pan Y,Han Y,Yang L,Xia J,Xu Fdoi
10.1111/cpr.12721subject
Has Abstractpub_date
2020-01-01 00:00:00pages
e12721issue
1eissn
0960-7722issn
1365-2184journal_volume
53pub_type
杂志文章abstract::Several methods to synchronize cultured cells in the cell cycle are based on temporary inhibition of DNA replication. Previously it has been reported that cells synchronized this way exhibited significant growth imbalance and unscheduled expression of cyclins A and B1. We have now observed that HL-60 cells exposed to ...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.2006.00380.x
更新日期:2006-06-01 00:00:00
abstract:OBJECTIVES:Silicate bioactive glass (BG) has been widely demonstrated to stimulate both of the hard and soft tissue regeneration, in which ion products released from BG play important roles. However, the mechanism by which ion products act on cells on cells is unclear. MATERIALS AND METHODS:Human umbilical vein endoth...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12906
更新日期:2020-11-01 00:00:00
abstract:OBJECTIVES:Oral cancer represents one of the most common malignancies in humans. Its prognosis is still poor, despite the most recent improvements in therapies. An increasing attention is placed on the role of programmed death ligand 1 (PD-L1) in the tumour immunity and its potential function as a marker for tumour pro...
journal_title:Cell proliferation
pub_type: 杂志文章,meta分析
doi:10.1111/cpr.12537
更新日期:2019-03-01 00:00:00
abstract:OBJECTIVES:Limbal stem cells (LSC) are self-renewing, highly proliferative cells in vitro, which express a set of specific markers and in vivo have the capacity to reconstruct the entire corneal epithelium in cases of ocular surface injury. Currently, LSC transplantation is a commonly used procedure in patients with ei...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.2009.00623.x
更新日期:2009-10-01 00:00:00
abstract:OBJECTIVE:It is not known whether or not epithelial progenitors of the pyloric antrum are involved in gastric carcinogenesis. Normally, these progenitors give rise to two main cell lineages: pit and gland mucous cells. This study was designed to examine the changes that occur in pyloric antral mucous cell lineages and ...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.2008.00562.x
更新日期:2008-12-01 00:00:00
abstract:OBJECTIVES:Both interleukin-6 (IL-6) and transforming growth factor-beta (TGF-beta) are crucially involved in fibrotic events that characterize interstitial lung diseases (ILD). Therefore, the aim of this study was to investigate in primary cultures of normal and fibrotic human lung fibroblasts (HLF), exposed to either...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.2008.00538.x
更新日期:2008-06-01 00:00:00
abstract:OBJECTIVES:Accumulating evidence demonstrated that the long noncoding RNA (lncRNA) HOTAIR (Hox transcript antisense intergenic RNA) plays key role in renal cell carcinoma (RCC) malignancy, while microRNA-124 (miR-124) is a tumour suppressor in RCC. The aim of this work was to assess the biological function of HOTAIR an...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12507
更新日期:2018-12-01 00:00:00
abstract::Breast cancer has seriously been threatening physical and mental health of women in the world, and its morbidity and mortality also show clearly upward trend in China over time. Through inquiry, we find that survival rate of patients with early-stage breast cancer is significantly higher than those with middle- and la...
journal_title:Cell proliferation
pub_type: 杂志文章,评审
doi:10.1111/cpr.12822
更新日期:2020-07-01 00:00:00
abstract:OBJECTIVES:Curcumin, a natural compound, is a potent anti-cancer agent, which inhibits cell division and/or induces cell death. It is believed that normal cells are less sensitive to curcumin than malignant cells; however, the mechanism(s) responsible for curcumin's effect on normal cells are poorly understood. The aim...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.2010.00684.x
更新日期:2010-08-01 00:00:00
abstract::Aberrant crypt foci (ACF) have been identified on the colonic mucosal surface of rodents treated with colon carcinogens and of humans after methylene-blue staining and observation under a light microscope. Several lines of evidence strongly suggest that ACF with certain morphological, histological, cell kinetics, and ...
journal_title:Cell proliferation
pub_type: 杂志文章,评审
doi:10.1046/j.1365-2184.2000.00159.x
更新日期:2000-02-01 00:00:00
abstract:OBJECTIVES:Cardiac atrial appendage stem cells (CASCs) have recently emerged as an attractive candidate for cardiac regeneration after myocardial infarction. As with other cardiac stem cells, CASCs have to be expanded ex vivo to obtain clinically relevant cell numbers. However, foetal calf serum (FCS), which is routine...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12166
更新日期:2015-04-01 00:00:00
abstract:OBJECTIVES:miR-21 can promote osteoblast differentiation of periodontal ligament stem cells. However, the effect of miR-21 on bone remodelling in the midpalatal suture is unclear. This study aimed to elucidate the effects of miR-21 on the midpalatal suture bone remodelling by expanding the palatal sutures. MATERIALS A...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12697
更新日期:2020-01-01 00:00:00
abstract:OBJECTIVES:Fibrotic cataract, including posterior capsule opacification (PCO) and anterior subcapsular cataract (ASC), renders millions of people visually impaired worldwide. However, the underlying mechanism remains poorly understood. Here, we report a miRNA-based regulatory pathway that controls pathological fibrosis...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12911
更新日期:2020-11-01 00:00:00
abstract::Telomere length plays an important role in regulating the proliferative capacity of cells, and serves as a marker for cell cycle history and also for their remaining replicative potential. Mesenchymal stromal cells (MSC) are known to be a significant cell source for therapeutic intervention and tissue engineering. To ...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.2006.00397.x
更新日期:2006-12-01 00:00:00
abstract:OBJECTIVE:Curcumin (capable of inhibiting angiogenic growth of human umbilical vein endothelial cells [HUVECs]), can be employed in vitro as a model of pathogenesis of corneal neovascularization (CRNV). The aim of this study was to explore regulatory mechanisms of microRNA (miR) levels after curcumin treatment. MATERI...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12289
更新日期:2016-12-01 00:00:00
abstract:OBJECTIVES:Beryllium salts (here, beryllium sulphate) can produce a cytostatic effect in some cell types. The basis for this effect may include increased expression of proliferation inhibitors, reduced expression of proliferation promoters, or both. This study sought to determine the role of p53, the tumour-suppressing...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12291
更新日期:2016-12-01 00:00:00
abstract::In situ presence of numerous DNA strand breaks is a typical feature of apoptotic cells. Selective DNA strand break induction by photolysis (SBIP) at sites that contain incorporated halogenated DNA precursors has recently been proposed as a method of analysing DNA replication. Detection of DNA strand breaks, thus, enab...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.1995.tb00045.x
更新日期:1995-11-01 00:00:00
abstract::The R- cell line is a 3T3-like cell line originating from mouse embryos with a homozygous disruption of the type 1 insulin-like growth factor receptor (IGF-IR) genes. Although R- cells cannot grow at all in serum-free medium (SFM) supplemented by several known growth factors, either singly or in combination, they are ...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:
更新日期:1997-06-01 00:00:00
abstract:OBJECTIVES:The main purpose of current study was to explore the effects of tetrahedral DNA nanostructures (TDNs) on neuroectodermal (NE-4C) stem cells migration and unveil the potential mechanisms. MATERIALS AND METHODS:The successfully self-assembled TDNs were also determined by dynamic light scattering (DLS). A bidi...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12503
更新日期:2018-12-01 00:00:00
abstract::In this paper we describe the application of a non-radioactive DNA double labelling and staining method to an analysis of cell proliferation kinetics by flow cytometry, aimed at the direct measurement of recruitment rates in cell cultures. The method is based on the application of two halogenated deoxyuridines: iodode...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.1993.tb00009.x
更新日期:1993-01-01 00:00:00
abstract:OBJECTIVES:The bone tissue engineering primarily focuses on three-dimensional co-culture systems, which physical and biological properties resemble the cell matrix of actual tissues. The complex dialogue between bone-forming and endothelial cells (ECs) in a tissue-engineered construct will directly regulate angiogenesi...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12658
更新日期:2019-09-01 00:00:00
abstract:OBJECTIVES:Worldwide, lung cancer accounts for the majority of cancer-related deaths. Aberrant expression of miRNAs has increasingly been reported to be associated with tumour progression. This study aimed to explore the role of miR-301b in regulating apoptosis in lung cancer. MATERIALS AND METHODS:Expression of miR-3...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12264
更新日期:2016-08-01 00:00:00
abstract:OBJECTIVES:Articular cartilage plays a vital role in bearing and buffering. Injured cartilage and subchondral bone repair is a crucial challenge in cartilage tissue engineering due to the peculiar structure of osteochondral unit and the requirement of osteogenic/chondrogenic bi-directional differentiation. Based on the...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12917
更新日期:2020-11-01 00:00:00
abstract:OBJECTIVES:BCL2 family proteins have been widely studied over the past decade due to their essential roles in apoptosis, oncogenesis and anti-cancer therapy. However, the similarities and differences in the spatial pattern of the BCL2 gene family within the context of chromatin have not been well characterized. We soug...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12826
更新日期:2020-06-01 00:00:00
abstract::Extracellular vesicles (EVs), a heterogeneous group of vesicles differing in size and shape, cargo content and function, are membrane-bound and nano-sized vesicles that could be released by nearly all variations of cells. EVs have gained considerable attention in the past decades for their functions in modulating inte...
journal_title:Cell proliferation
pub_type: 杂志文章,评审
doi:10.1111/cpr.12659
更新日期:2019-11-01 00:00:00
abstract:OBJECTIVES:Often, non-small cell lung cancers (NSCLC) respond only poorly to the tyrosine kinase inhibitor (TKI) gefitinib, which targets the epidermal growth factor receptor (EGFR), these poor responders EGFRs lacking activating mutations. In this study, we have attempted to improve TKI response of NSCLC cell lines (A...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12040
更新日期:2013-08-01 00:00:00
abstract:OBJECTIVE:Management of chondral defects has long been a challenge due to poor self-healing capacity of articular cartilage. Many approaches, ranging from symptomatic treatment to structural cartilage regeneration, have obtained very limited satisfactory results. Cartilage tissue engineering, which involves optimized c...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12295
更新日期:2016-12-01 00:00:00
abstract:OBJECTIVES:Advanced head and neck carcinomas (HNCs) are aggressive tumours, mainly due to hypoxia and a cancer stem cell (CSC) subpopulation. The aim of this study was to simulate tumour growth and behaviour during radiotherapy of three HNC groups (governed by different growth kinetics, hypoxia levels and CSC division ...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12251
更新日期:2016-06-01 00:00:00
abstract:OBJECTIVES:To assess the expression, prognostic value, and functionality of T-lymphokine-activated killer (T-LAK) cell-originated protein kinase (TOPK) in chordoma pathogenesis. MATERIALS AND METHODS:TOPK expression in chordoma was assessed via immunohistochemical staining of a tissue microarray (TMA) and correlated w...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12901
更新日期:2020-10-01 00:00:00
abstract::Triple-negative breast cancer (TNBC) is one of the most aggressive subtypes of breast cancer with negativity for oestrogen receptor (ER), progesterone receptor (PR) and human epidermal growth factor receptor (HER2). Non-coding RNAs (ncRNAs) make up most of the transcriptome and are widely present in eukaryotic cells. ...
journal_title:Cell proliferation
pub_type: 杂志文章,评审
doi:10.1111/cpr.12801
更新日期:2020-05-01 00:00:00