PCL-PEG-PCL film promotes cartilage regeneration in vivo.


OBJECTIVE:Management of chondral defects has long been a challenge due to poor self-healing capacity of articular cartilage. Many approaches, ranging from symptomatic treatment to structural cartilage regeneration, have obtained very limited satisfactory results. Cartilage tissue engineering, which involves optimized combination of novel scaffolds, cell sources and growth factors, has emerged as a promising strategy for cartilage regeneration and repair. In this study, the aim was to investigate the role of poly(ε-caprolactone)-poly(ethylene glycol)-poly(ε-caprolactone) (PCL-PEG-PCL, PCEC) PCEC scaffold in cartilage repair. MATERIALS AND METHODS:First, PCEC film was fabricated, and its characteristics were tested using SEM and AFM. Cell (rASC - rat adipose-derived stem cells, and mASCs - green fluorescent mouse adipose-derived stem cells) morphologies on PCEC film were observed using SEM and fluorescence microscopy, after cell seeding. Tests of cell viability on PCEC film were conducted using the CCK-8 assay. Furthermore, full cartilage defects in rats were created, and PCEC films were implanted, to evaluate their healing effects, over 8 weeks. RESULTS:It was found that PCEC film, as a biomaterial implant, possessed good in vitro properties for cell adhesion, migration and proliferation. Importantly, in the in vivo experiment, PCEC film exhibited desirable healing outcomes. CONCLUSIONS:These results demonstrated that PCEC film was a good scaffold for cartilage tissue engineering for improving cell proliferation and adhesion and could lead to excellent repair of cartilage defects.


Cell Prolif


Cell proliferation


Fu N,Liao J,Lin S,Sun K,Tian T,Zhu B,Lin Y




Has Abstract


2016-12-01 00:00:00












  • Downregulation of USP32 inhibits cell proliferation, migration and invasion in human small cell lung cancer.

    abstract:OBJECTIVES:Ubiquitin specific protease 32 (USP32) is a highly conserved but uncharacterized gene, which has been reported to be associated with growth of breast cancer cells. However, the role of USP32 in human small cell lung cancer (SCLC) has not been uncovered. The aim of this study was to investigate and evaluate t...

    journal_title:Cell proliferation

    pub_type: 杂志文章


    authors: Hu W,Wei H,Li K,Li P,Lin J,Feng R

    更新日期:2017-08-01 00:00:00

  • SAV1 represses the development of human colorectal cancer by regulating the Akt-mTOR pathway in a YAP-dependent manner.

    abstract:OBJECTIVES:SAV1 is a human homologue of Salvador that contains two protein-protein interaction modules known as WW domains and acts as a scaffolding protein. SAV1 participates in the development of diverse types of cancer. We aimed to investigate the role of SAV1 in human colorectal cancer. MATERIALS AND METHODS:Human...

    journal_title:Cell proliferation

    pub_type: 杂志文章


    authors: Jiang J,Chang W,Fu Y,Gao Y,Zhao C,Zhang X,Zhang S

    更新日期:2017-08-01 00:00:00

  • In silico study of the impact of cancer stem cell dynamics and radiobiological hypoxia on tumour response to hyperfractionated radiotherapy.

    abstract:OBJECTIVES:Advanced head and neck carcinomas (HNCs) are aggressive tumours, mainly due to hypoxia and a cancer stem cell (CSC) subpopulation. The aim of this study was to simulate tumour growth and behaviour during radiotherapy of three HNC groups (governed by different growth kinetics, hypoxia levels and CSC division ...

    journal_title:Cell proliferation

    pub_type: 杂志文章


    authors: Marcu LG,Marcu D,Filip SM

    更新日期:2016-06-01 00:00:00

  • Induction of retinal-dependent calcium influx in human melanocytes by UVA or UVB radiation contributes to the stimulation of melanosome transfer.

    abstract:OBJECTIVES:The transfer of melanosomes from melanocytes to neighbouring keratinocytes is critical to protect the skin from the deleterious effects of ultraviolet A (UVA) and ultraviolet B (UVB) irradiation; however, the initial factor(s) that stimulates melanosome transfer remains unclear. In this study, we investigate...

    journal_title:Cell proliferation

    pub_type: 杂志文章


    authors: Hu QM,Yi WJ,Su MY,Jiang S,Xu SZ,Lei TC

    更新日期:2017-12-01 00:00:00

  • Computer-aided identification of a novel pyruvate kinase M2 activator compound.

    abstract:OBJECTIVES:The aim of this study was to obtain antitumour molecules targeting to activate PKM2 through adequate computational methods combined with biological activity experiments. METHODS:The structure-based virtual screening was utilized to screen effective activator targeting PKM2 from ZINC database. Molecular dyna...

    journal_title:Cell proliferation

    pub_type: 杂志文章


    authors: Li Y,Bao M,Yang C,Chen J,Zhou S,Sun R,Wu C,Li X,Bao J

    更新日期:2018-12-01 00:00:00

  • Characterization and enumeration of spontaneously proliferating human leucocytes by multiparameter flow cytometry.

    abstract::Spontaneously proliferating human leucocytes have been characterized and enumerated using multiparameter flow cytometry. The frequency of spontaneously proliferating cells amongst human peripheral blood mononuclear cells, was determined on the basis of BrdUrd incorporation and total DNA content in samples of cells inc...

    journal_title:Cell proliferation

    pub_type: 杂志文章


    authors: Johannisson A,Amnéus H

    更新日期:1993-07-01 00:00:00

  • Expression and role of Notch signalling in the regeneration of rat tracheal epithelium.

    abstract:OBJECTIVES:This study is to explore the role of Notch signalling during the regeneration of rat tracheal epithelium after injury induced by 5-fluorouracil (5-FU). MATERIALS AND METHODS:We developed an ex vivo model of rat tracheal epithelial regeneration using 5-FU to induce injury. Expression levels of members of the...

    journal_title:Cell proliferation

    pub_type: 杂志文章


    authors: Ma XB,Jia XS,Liu YL,Wang LL,Sun SL,Song N,Wang EH,Li F

    更新日期:2009-02-01 00:00:00

  • Chondrocyte proliferation in a new culture system.

    abstract:OBJECTIVE:This study has aimed to study different culture systems that might stimulate an increase in cell proliferation of normal and osteoarthritis chondrocytes from articular cartilage in rat model. MATERIAL AND METHODS:Three culture systems using chondrocytes embedded in alginate beads were tested: chondrocytes cu...

    journal_title:Cell proliferation

    pub_type: 杂志文章


    authors: Gomez-Camarillo MA,Almonte-Becerril M,Vasquez Tort M,Tapia-Ramirez J,Kouri Flores JB

    更新日期:2009-04-01 00:00:00

  • Nicotine-induced proliferation of isolated rat pancreatic acinar cells: effect on cell signalling and function.

    abstract:OBJECTIVES:The aim of the current study was to investigate whether nicotine treatment would induce the proliferation of isolated rat primary pancreatic acinar cells in culture by activating mitogen-activated protein kinase (MAPK) signalling and exocrine secretion. MATERIALS AND METHODS:A nicotine dose- and time-respon...

    journal_title:Cell proliferation

    pub_type: 杂志文章


    authors: Chowdhury P,Bose C,Udupa KB

    更新日期:2007-02-01 00:00:00

  • Towards responsible cord blood banking models.

    abstract::On 31 May 2010, 14 072 567 bone marrow/apheresis donors registered in 44 countries and 426 501 cord blood units banked in 26 countries for public use were available to treat candidates to haemopoietic stem cell transplant lacking a family related compatible donor. Despite these impressive numbers, additional efforts a...

    journal_title:Cell proliferation

    pub_type: 杂志文章


    authors: Rebulla P,Lecchi L

    更新日期:2011-04-01 00:00:00

  • Clinical-scale in vitro expansion preserves biological characteristics of cardiac atrial appendage stem cells.

    abstract:OBJECTIVES:Cardiac atrial appendage stem cells (CASCs) have recently emerged as an attractive candidate for cardiac regeneration after myocardial infarction. As with other cardiac stem cells, CASCs have to be expanded ex vivo to obtain clinically relevant cell numbers. However, foetal calf serum (FCS), which is routine...

    journal_title:Cell proliferation

    pub_type: 杂志文章


    authors: Windmolders S,Willems L,Daniëls A,Linsen L,Fanton Y,Hendrikx M,Koninckx R,Rummens JL,Hensen K

    更新日期:2015-04-01 00:00:00

  • Glucagon-like peptide 2 decreases osteoclasts by stimulating apoptosis dependent on nitric oxide synthase.

    abstract:OBJECTIVES:Glucagon-like peptide 2 (GLP2) is involved in the regulation of energy absorption and metabolism. Despite the importance of the GLP2 signalling mechanisms on osteoclast, little has been studied on how GLP2 works during osteoclastogenesis. MATERIALS AND METHODS:RAW264.7 cells were infected with rLV-Green-GLP...

    journal_title:Cell proliferation

    pub_type: 杂志文章


    authors: Lu Y,Lu D,Hu Y

    更新日期:2018-08-01 00:00:00

  • MicroRNA-21 affects mechanical force-induced midpalatal suture remodelling.

    abstract:OBJECTIVES:miR-21 can promote osteoblast differentiation of periodontal ligament stem cells. However, the effect of miR-21 on bone remodelling in the midpalatal suture is unclear. This study aimed to elucidate the effects of miR-21 on the midpalatal suture bone remodelling by expanding the palatal sutures. MATERIALS A...

    journal_title:Cell proliferation

    pub_type: 杂志文章


    authors: Li M,Zhang Z,Gu X,Jin Y,Feng C,Yang S,Wei F

    更新日期:2020-01-01 00:00:00

  • Gamabufotalin induces a negative feedback loop connecting ATP1A3 expression and the AQP4 pathway to promote temozolomide sensitivity in glioblastoma cells by targeting the amino acid Thr794.

    abstract:OBJECTIVES:Temozolomide (TMZ) is one of the most commonly used clinical drugs for glioblastoma (GBM) treatment, but its drug sensitivity needs to be improved. Gamabufotalin (CS-6), the primary component of the traditional Chinese medicine "ChanSu," was shown to have strong anti-cancer activity. However, more efforts sh...

    journal_title:Cell proliferation

    pub_type: 杂志文章


    authors: Lan YL,Chen C,Wang X,Lou JC,Xing JS,Zou S,Hu JL,Lyu W,Zhang B

    更新日期:2020-01-01 00:00:00

  • IGF-1 promotes angiogenesis in endothelial cells/adipose-derived stem cells co-culture system with activation of PI3K/Akt signal pathway.

    abstract:OBJECTIVES:The aim of this study was to investigate the role of insulin-like growth factor-1 (IGF-1) and crosstalk between endothelial cells (ECs) and adipose-derived stem cells (ASCs) in the process of angiogenesis. METHODS:A three-dimensional collagen gel used to culture mouse ASCs and mouse ECs in vitro was establi...

    journal_title:Cell proliferation

    pub_type: 杂志文章


    authors: Lin S,Zhang Q,Shao X,Zhang T,Xue C,Shi S,Zhao D,Lin Y

    更新日期:2017-12-01 00:00:00

  • Expression of the 170-kDa and 180-kDa isoforms of DNA topoisomerase II in resting and proliferating human lymphocytes.

    abstract::The expression of the 170-kDa (alpha) and the 180-kDa (beta) isoforms of DNA topoisomerase II (topo II) was investigated with two specific monoclonal antibodies (MoAbs) in human peripheral blood lymphocytes (PBL), before and after phytohaemoagglutinin (PHA) stimulation. Binding of each MoAb was detected by indirect im...

    journal_title:Cell proliferation

    pub_type: 杂志文章


    authors: Prosperi E,Negri C,Marchese G,Ricotti GC

    更新日期:1994-05-01 00:00:00

  • Osteoclastogenesis inhibition by mutated IGSF23 results in human osteopetrosis.

    abstract:OBJECTIVES:Osteopetrosis is a rare inherited skeletal disease characterized by increased bone mineral density due to the loss of osteoclast function or differentiation potential. MATERIALS AND METHODS:The study involved a Chinese patient with osteopetrosis (the proband) and her immediate family members and 180 control...

    journal_title:Cell proliferation

    pub_type: 杂志文章


    authors: Yuan Y,Yang L,Liu T,Zhang H,Lu Q

    更新日期:2019-11-01 00:00:00

  • miR-137 and its target T-type CaV 3.1 channel modulate dedifferentiation and proliferation of cerebrovascular smooth muscle cells in simulated microgravity rats by regulating calcineurin/NFAT pathway.

    abstract:OBJECTIVES:Postflight orthostatic intolerance has been regarded as a major adverse effect after microgravity exposure, in which cerebrovascular adaptation plays a critical role. Our previous finding suggested that dedifferentiation of vascular smooth muscle cells (VSMCs) might be one of the key contributors to cerebrov...

    journal_title:Cell proliferation

    pub_type: 杂志文章


    authors: Zhang B,Chen L,Bai YG,Song JB,Cheng JH,Ma HZ,Ma J,Xie MJ

    更新日期:2020-03-01 00:00:00

  • Cell kinetic studies in mouse tongue mucosa by autoradiographic, immunohistochemical and flow cytometric techniques.

    abstract::A number of techniques, including autoradiography after in vivo administration of tritiated thymidine ([3H]dT), immunohistochemistry after in vivo administration of bromodeoxyuridine (BrdUrd), and flow cytometry (FCM) with and without BrdUrd detection were compared in the epithelium of ventral mouse tongue. Investigat...

    journal_title:Cell proliferation

    pub_type: 杂志文章


    authors: Dörr W,Arui J,Beisker W,Schultz-Hector S

    更新日期:1994-06-01 00:00:00

  • Measurement of cell proliferation by enzyme-linked immunosorbent assay (ELISA) using a monoclonal antibody to bromodeoxyuridine.

    abstract::An ELISA was developed and optimized to measure cell proliferation using a monoclonal antibody to bromodeoxyuridine (BrdUrd). Incorporation of BrdUrd into myoblast monolayers, measured as the optical density at 492 nm, increased in response to fetal calf serum, IGF-I and EGF, the ELISA data correlated closely with dat...

    journal_title:Cell proliferation

    pub_type: 杂志文章


    authors: Perros P,Weightman DR

    更新日期:1991-09-01 00:00:00

  • Different effects of ribosome biogenesis inhibition on cell proliferation in retinoblastoma protein- and p53-deficient and proficient human osteosarcoma cell lines.

    abstract:OBJECTIVES:To evaluate the effects of rRNA synthesis inhibition on cell cycle progression and cell population growth according to the RB and p53 status. MATERIAL AND METHODS:RB- and p53-proficient U2OS cells and the RB- and p53-deficient SAOS-2 cells were used, rRNA transcription hindered by actinomycin D, and cell cy...

    journal_title:Cell proliferation

    pub_type: 杂志文章


    authors: Montanaro L,Mazzini G,Barbieri S,Vici M,Nardi-Pantoli A,Govoni M,Donati G,Treré D,Derenzini M

    更新日期:2007-08-01 00:00:00

  • Histone deacetylase inhibitors decrease proliferation potential and multilineage differentiation capability of human mesenchymal stem cells.

    abstract:OBJECTIVES:Histone deacetylase (HDAC) is an important therapeutic target in cancer. Two of the main anticancer mechanisms of HDAC inhibitors are induction of terminal differentiation and inhibition of cell proliferation. To investigate the role of HDAC in maintenance of self-renewal and cell proliferation, we treated m...

    journal_title:Cell proliferation

    pub_type: 杂志文章


    authors: Lee S,Park JR,Seo MS,Roh KH,Park SB,Hwang JW,Sun B,Seo K,Lee YS,Kang SK,Jung JW,Kang KS

    更新日期:2009-12-01 00:00:00

  • Effects of serum reduction and VEGF supplementation on angiogenic potential of human adipose stromal cells in vitro.

    abstract:OBJECTIVES:This study investigated effects of reduced serum condition and vascular endothelial growth factor (VEGF) on angiogenic potential of adipose stromal cells (ASCs) in vitro. MATERIALS AND METHODS:Adipose stromal cells were cultured in three different types of medium: (i) F12/DMEM (FD) supplemented with 10% FBS...

    journal_title:Cell proliferation

    pub_type: 杂志文章


    authors: Chua KH,Raduan F,Wan Safwani WK,Manzor NF,Pingguan-Murphy B,Sathapan S

    更新日期:2013-06-01 00:00:00

  • Labelling DNA strand breaks with BrdUTP. Detection of apoptosis and cell proliferation.

    abstract::In situ presence of numerous DNA strand breaks is a typical feature of apoptotic cells. Selective DNA strand break induction by photolysis (SBIP) at sites that contain incorporated halogenated DNA precursors has recently been proposed as a method of analysing DNA replication. Detection of DNA strand breaks, thus, enab...

    journal_title:Cell proliferation

    pub_type: 杂志文章


    authors: Li X,Darzynkiewicz Z

    更新日期:1995-11-01 00:00:00

  • Hypoxia-induced microRNA-301b regulates apoptosis by targeting Bim in lung cancer.

    abstract:OBJECTIVES:Worldwide, lung cancer accounts for the majority of cancer-related deaths. Aberrant expression of miRNAs has increasingly been reported to be associated with tumour progression. This study aimed to explore the role of miR-301b in regulating apoptosis in lung cancer. MATERIALS AND METHODS:Expression of miR-3...

    journal_title:Cell proliferation

    pub_type: 杂志文章


    authors: Wu D,Chen B,Cui F,He X,Wang W,Wang M

    更新日期:2016-08-01 00:00:00

  • Insulin promotes invasion and migration of KRASG12D mutant HPNE cells by upregulating MMP-2 gelatinolytic activity via ERK- and PI3K-dependent signalling.

    abstract:OBJECTIVES:Hyperinsulinemia is a risk factor for pancreatic cancer, but the function of insulin in carcinogenesis is unclear, so this study aimed to elucidate the carcinogenic effects of insulin and the synergistic effect with the KRAS mutation in the early stage of pancreatic cancer. MATERIALS AND METHODS:A pair of i...

    journal_title:Cell proliferation

    pub_type: 杂志文章


    authors: Wang G,Yin L,Peng Y,Gao Y,Gao H,Zhang J,Lv N,Miao Y,Lu Z

    更新日期:2019-05-01 00:00:00

  • Culture and in vitro hepatogenic differentiation of placenta-derived stem cells, using placental extract as an alternative to serum.

    abstract:OBJECTIVES:Translational research using adult stem cells derived from various tissues has been highlighted in cell-based therapy. However, there are many limitations to using conventional culture systems of adult stem cells for clinically applicability, including limited combinations of cytokines and use of nutrients d...

    journal_title:Cell proliferation

    pub_type: 杂志文章


    authors: Shin KS,Lee HJ,Jung J,Cha DH,Kim GJ

    更新日期:2010-10-01 00:00:00

  • Possibility of selection of chondrogenic progenitor cells by telomere length in FGF-2-expanded mesenchymal stromal cells.

    abstract::Telomere length plays an important role in regulating the proliferative capacity of cells, and serves as a marker for cell cycle history and also for their remaining replicative potential. Mesenchymal stromal cells (MSC) are known to be a significant cell source for therapeutic intervention and tissue engineering. To ...

    journal_title:Cell proliferation

    pub_type: 杂志文章


    authors: Yanada S,Ochi M,Kojima K,Sharman P,Yasunaga Y,Hiyama E

    更新日期:2006-12-01 00:00:00

  • Schisandrin A restrains osteoclastogenesis by inhibiting reactive oxygen species and activating Nrf2 signalling.

    abstract:OBJECTIVES:Intracellular reactive oxygen species (ROS) induced by receptor activator of NF-kB ligand (RANKL) has been proven to be a critical factor in the development of osteoclasts. This study aimed to prove that schisandrin A (Sch), a novel anti-oxidant compound, is able to suppress osteoclastogenesis and prevent bo...

    journal_title:Cell proliferation

    pub_type: 杂志文章


    authors: Ni S,Qian Z,Yuan Y,Li D,Zhong Z,Ghorbani F,Zhang X,Zhang F,Zhang Z,Liu Z,Yu B

    更新日期:2020-10-01 00:00:00

  • Mechanisms of Panax ginseng action as an antidepressant.

    abstract:OBJECTIVES:Panax ginseng, a well-known traditional Chinese medicine with multiple pharmacological activities, plays a crucial role in modulating mood disorders. Several recent studies have identified an underlying role of Panax ginseng in the prevention and treatment of depression. However, the cellular and molecular m...

    journal_title:Cell proliferation

    pub_type: 杂志文章,评审


    authors: Jin Y,Cui R,Zhao L,Fan J,Li B

    更新日期:2019-11-01 00:00:00