Abstract:
OBJECTIVES:Limbal stem cells (LSC) are self-renewing, highly proliferative cells in vitro, which express a set of specific markers and in vivo have the capacity to reconstruct the entire corneal epithelium in cases of ocular surface injury. Currently, LSC transplantation is a commonly used procedure in patients with either uni- or bilateral total limbal stem cells deficiency (TLSCD). Although LSC transplantation holds great promise for patients, several problems need to be overcome. In order to find an alternative source of cells that can partially substitute LSC in cornea epithelium reconstruction, we aimed at investigating whether human immature dental pulp stem cells (hIDPSC) would present similar key characteristics as LSC and whether they could be used for corneal surface reconstruction in a rabbit TLSCD model. MATERIALS:We used hIDPSC, which co-express mesenchymal and embryonic stem cell markers and present the capacity to differentiate into derivative cells of the three germinal layers. TLSCD was induced by chemical burn in one eye of rabbits. After 30 days, the opaque tissue formed was removed by superficial keratectomy. Experimental group received undifferentiated hIDPSC, while control group only received amniotic membrane (AM). Both groups were sacrificed after 3 months. RESULTS AND CONCLUSIONS:We have demonstrated, using immunohistochemistry and reverse transcription-polymerase chain reaction, that hIDPSCs express markers in common with LSC, such as ABCG2, integrin beta1, vimentin, p63, connexin 43 and cytokeratins 3/12. They were also capable of reconstructing the eye surface after induction of unilateral TLSCD in rabbits, as shown by morphological and immunohistochemical analysis using human-specific antibodies against limbal and corneal epithelium. Our data suggest that hIDPSCs share similar characteristics with LSC and might be used as a potential alternative source of cells for corneal reconstruction.
journal_name
Cell Prolifjournal_title
Cell proliferationauthors
Monteiro BG,Serafim RC,Melo GB,Silva MC,Lizier NF,Maranduba CM,Smith RL,Kerkis A,Cerruti H,Gomes JA,Kerkis Idoi
10.1111/j.1365-2184.2009.00623.xsubject
Has Abstractpub_date
2009-10-01 00:00:00pages
587-94issue
5eissn
0960-7722issn
1365-2184pii
CPR623journal_volume
42pub_type
杂志文章abstract::The R- cell line is a 3T3-like cell line originating from mouse embryos with a homozygous disruption of the type 1 insulin-like growth factor receptor (IGF-IR) genes. Although R- cells cannot grow at all in serum-free medium (SFM) supplemented by several known growth factors, either singly or in combination, they are ...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:
更新日期:1997-06-01 00:00:00
abstract:OBJECTIVES:Oestrogen is known to inhibit osteoclastogenesis, and numerous studies have identified it as an autophagic activator. To date, the role of oestrogen in the autophagy of osteoclast precursors (OCPs) during osteoclastogenesis remains unclear. This study aimed to determine the effect of autophagy regulated by t...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12789
更新日期:2020-04-01 00:00:00
abstract:OBJECTIVES:Accumulating evidence demonstrated that the long noncoding RNA (lncRNA) HOTAIR (Hox transcript antisense intergenic RNA) plays key role in renal cell carcinoma (RCC) malignancy, while microRNA-124 (miR-124) is a tumour suppressor in RCC. The aim of this work was to assess the biological function of HOTAIR an...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12507
更新日期:2018-12-01 00:00:00
abstract:OBJECTIVE:MicroRNAs (miRNAs) are negative regulators of gene expression that play important roles in cell processes such as proliferation, development and differentiation. Recently, it has been reported that miRNAs are related to development of carcinogenesis. The aim of this study was to identify miRNAs associated wit...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.2010.00717.x
更新日期:2011-02-01 00:00:00
abstract:OBJECTIVES:Based on in vivo studies, low-intensity pulsed ultrasound (LIPUS) stimulation has been widely used in the clinic for advancing bone growth during healing of non-union alignment, fractures and other osseous defects. In this study, we have investigated osteogenic differentiation of adipose stem cells (ASCs) re...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12035
更新日期:2013-06-01 00:00:00
abstract:OBJECTIVES:Mesenchymal stem cells (MSCs) are frequently used for bone regeneration, however, they are limited in quantity. Moreover, their proliferation and differentiation capabilities reduce during cell culture expansion. Potential application of induced pluripotent stem cells (iPSCs) has been reported as a promising...
journal_title:Cell proliferation
pub_type: 杂志文章,评审
doi:10.1111/cpr.12321
更新日期:2017-04-01 00:00:00
abstract:OBJECTIVES:The role of stem cells in regenerative medicine is evolving rapidly. Here, we describe the application, for kidney regeneration, of a novel non-genetically modified stem cell, derived from human amniotic fluid. We show that these pluripotent cells can develop and differentiate into de novo kidney structures ...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.2007.00478.x
更新日期:2007-12-01 00:00:00
abstract:OBJECTIVES:To investigate the role of hierarchical micro/nanoscale topography of direct metal laser sintering (DMLS) titanium surfaces in osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs), as well as the possible underlying epigenetic mechanism. MATERIALS AND METHODS:Three groups of titanium spe...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12460
更新日期:2018-08-01 00:00:00
abstract::Studies on growth regulation in vitro to a large extent rely on comparison of growth curves. However, these do not discriminate between the relative contributions of the mitotic rate and the apoptotic rate to the net growth rate. In the present study, differential effects of 17beta-oestradiol (E2, 10(-8) M) and/or tam...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1046/j.1365-2184.1999.3250289.x
更新日期:1999-10-01 00:00:00
abstract:OBJECTIVES:The transfer of melanosomes from melanocytes to neighbouring keratinocytes is critical to protect the skin from the deleterious effects of ultraviolet A (UVA) and ultraviolet B (UVB) irradiation; however, the initial factor(s) that stimulates melanosome transfer remains unclear. In this study, we investigate...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12372
更新日期:2017-12-01 00:00:00
abstract::The epithelium of the oral cavity and small intestine of the gastrointestinal tract have a high rate of cell renewal and as such, are sensitive to cytotoxic therapies that kill rapidly dividing cells. Mucositis is a complication of cancer therapy where impairment of the regenerative capacity of the epithelium leads to...
journal_title:Cell proliferation
pub_type: 杂志文章,评审
doi:10.1046/j.1365-2184.35.s1.8.x
更新日期:2002-08-01 00:00:00
abstract::RAIDD (RIP-associated ICH-1 homologous protein with a death domain) is an adaptor molecule that mediates the action of cysteine proteases involved in apoptosis. To study the possibility of a novel system of cell ablation mediated by RAIDD, a preadipocyte cell line (3T3L1) was stably transfected with a plasmid containi...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1046/j.1365-2184.2003.00253.x
更新日期:2003-02-01 00:00:00
abstract::A mathematical model for the cell cycle is proposed that incorporates the known biochemical reactions involving both cyclin A and cyclin B, the interactions of these cyclins with cdc2 and cdk2, and the controlling effects of cdc25 and weel. The model also postulates the existence of an as yet unknown phosphatase invol...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.1994.tb01409.x
更新日期:1994-02-01 00:00:00
abstract::In the past few years, the paramount role of cancer stem cells (CSCs), in terms of cancer initiation, proliferation, metastasis, invasion and chemoresistance, has been revealed by accumulating studies. However, this level of cellular plasticity cannot be entirely explained by genetic mutations. Research on epigenetic ...
journal_title:Cell proliferation
pub_type: 杂志文章,评审
doi:10.1111/cpr.12963
更新日期:2020-12-13 00:00:00
abstract:OBJECTIVES:Histone deacetylase (HDAC) is an important therapeutic target in cancer. Two of the main anticancer mechanisms of HDAC inhibitors are induction of terminal differentiation and inhibition of cell proliferation. To investigate the role of HDAC in maintenance of self-renewal and cell proliferation, we treated m...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.2009.00633.x
更新日期:2009-12-01 00:00:00
abstract::Atherosclerosis is an inflammatory-fibroproliferative response of the arterial wall involving a complex set of interconnected events where cell proliferation (lymphomonocytes, and endothelial and smooth-muscle cells) and substantial perturbations of intracellular cholesterol metabolism are considered to be among the m...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1046/j.1365-2184.2002.00231.x
更新日期:2002-06-01 00:00:00
abstract:OBJECTIVES:To assess the expression, prognostic value, and functionality of T-lymphokine-activated killer (T-LAK) cell-originated protein kinase (TOPK) in chordoma pathogenesis. MATERIALS AND METHODS:TOPK expression in chordoma was assessed via immunohistochemical staining of a tissue microarray (TMA) and correlated w...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12901
更新日期:2020-10-01 00:00:00
abstract:OBJECTIVE:Accumulating data show that dysregulation of long noncoding RNAs (lncRNAs) acts a critical role in a variety of malignancies. Among these lncRNAs, small nucleolar RNA host genes (SNHGs) are associated with tumour growth and progression. But, the molecular mechanisms by which SNHG4 contributes to osteosarcoma ...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12515
更新日期:2018-12-01 00:00:00
abstract:OBJECTIVES:Enterochromaffin (EC) cells have been associated with functional gastrointestinal disorders such as IBS. Recently, we found that glial cell-derived neurotrophic factor (GDNF)-rearranged during transfection (RET) localized in EC cells in human colonic epithelia. Here, we examine the role of GDNF-RET in the pa...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12889
更新日期:2020-10-01 00:00:00
abstract:OBJECTIVES:MicroRNAs (miRNAs) as small non-coding RNA molecules act by negatively regulating their target genes. Recent studies have shown that protein phosphatase Mg2+/Mn2+-dependent 1F (PPM1F) plays a critical role in cancer metastasis. But, the regulation mechanisms of PPM1F by miRNAs in gastric cancer (GC) remain u...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12444
更新日期:2018-08-01 00:00:00
abstract::Triple-negative breast cancer (TNBC) is one of the most aggressive subtypes of breast cancer with negativity for oestrogen receptor (ER), progesterone receptor (PR) and human epidermal growth factor receptor (HER2). Non-coding RNAs (ncRNAs) make up most of the transcriptome and are widely present in eukaryotic cells. ...
journal_title:Cell proliferation
pub_type: 杂志文章,评审
doi:10.1111/cpr.12801
更新日期:2020-05-01 00:00:00
abstract::The cell cycle is controlled by numerous mechanisms ensuring correct cell division. This review will focus on these mechanisms, i.e. regulation of cyclin-dependent kinases (CDK) by cyclins, CDK inhibitors and phosphorylating events. The quality checkpoints activated after DNA damage are also discussed. The complexity ...
journal_title:Cell proliferation
pub_type: 杂志文章,评审
doi:10.1046/j.1365-2184.2003.00266.x
更新日期:2003-06-01 00:00:00
abstract::Chronic kidney disease (CKD) is a severe health problem worldwide, and vascular calcification (VC) contributes substantially to the cardiovascular morbidity and high mortality of CKD. CKD is often accompanied by a variety of pathophysiological states, such as inflammation, oxidative stress, hyperglycaemia, hyperparath...
journal_title:Cell proliferation
pub_type: 杂志文章,评审
doi:10.1111/cpr.12980
更新日期:2021-01-27 00:00:00
abstract::The CD133 glycoprotein is a controversial cancer stem cell marker in the field of neuro-oncology, based largely on the now considerable experimental evidence for the existence of both CD133+ve and CD133-ve populations as tumour-initiating cells. It is thought that decreasing oxygen tension enhances the complex regulat...
journal_title:Cell proliferation
pub_type: 杂志文章,评审
doi:10.1111/j.1365-2184.2012.00842.x
更新日期:2012-12-01 00:00:00
abstract:OBJECTIVES:Beryllium salts (here, beryllium sulphate) can produce a cytostatic effect in some cell types. The basis for this effect may include increased expression of proliferation inhibitors, reduced expression of proliferation promoters, or both. This study sought to determine the role of p53, the tumour-suppressing...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12291
更新日期:2016-12-01 00:00:00
abstract:OBJECTIVES:Exposure to microgravity induces many adaptive and pathological changes in human bone marrow mesenchymal stem cells (hBMSCs). However, the underlying mechanisms of these changes are poorly understood. We revealed the gene expression patterns of hBMSCs under normal ground (NG) and simulated microgravity (SMG)...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12539
更新日期:2019-03-01 00:00:00
abstract:OBJECTIVES:Temozolomide (TMZ) is one of the most commonly used clinical drugs for glioblastoma (GBM) treatment, but its drug sensitivity needs to be improved. Gamabufotalin (CS-6), the primary component of the traditional Chinese medicine "ChanSu," was shown to have strong anti-cancer activity. However, more efforts sh...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12732
更新日期:2020-01-01 00:00:00
abstract::Autophagy follows a lysosomal degradation pathway in which a cell digests its own components. It is highly regulated by a limited number of autophagy-related genes (Atg) and the proteins they encode, that are crucial for cells to undergo the process via modulating autophagsome formation. Recently, accumulating evidenc...
journal_title:Cell proliferation
pub_type: 杂志文章,评审
doi:10.1111/cpr.12095
更新日期:2014-04-01 00:00:00
abstract:OBJECTIVES:To investigate behaviour and osteogenic cytokine expression of RAW264.7 macrophages grown on TiO2 nanotube layers. MATERIALS AND METHODS:The murine macrophage cell line RAW 264.7 was cultured on TiO2 nanotubes of varying diameter; macrophage morphology was examined using scanning electron microscopy. Cell a...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12072
更新日期:2013-12-01 00:00:00
abstract::The human skin equivalent (HSE) is an in vitro reconstructed model that resembles skin morphologically and biochemically. The HSE is formed by overlaying a fibroblast-populated collagen matrix with a suspension of epidermal cells. Basal keratinocytes attach to the dermal equivalent via a newly formed basement membrane...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.1996.tb00981.x
更新日期:1996-12-01 00:00:00