Abstract:
OBJECTIVES:Based on in vivo studies, low-intensity pulsed ultrasound (LIPUS) stimulation has been widely used in the clinic for advancing bone growth during healing of non-union alignment, fractures and other osseous defects. In this study, we have investigated osteogenic differentiation of adipose stem cells (ASCs) regulated by LIPUS, and also in a preliminarily manner, we have discussed diverse effects of different duty ratio parameters. MATERIALS AND METHODS:Mouse adipose stem cells were isolated and osteogenically induced. Then they were treated with LIPUS for 10 min/day for 3 days, 5 days and 7 days, respectively. Finally, effects of LIPUS on osteogenic differentiation of the ASCs were analysed by real-time PCR, western blotting and immunofluorescence. RESULTS:Our data indicated that LIPUS promoted mRNA levels of runt-related transcription factor 2, osteopontin and osterix in the presence of osteo-induction medium; moreover, protein levels of runt-related transcription factor 2 and osteopontin were upregulated. CONCLUSIONS:We successfully demonstrated that LIPUS enhanced osteogenesis of ASCs, specially at the duty ratio of 20%.
journal_name
Cell Prolifjournal_title
Cell proliferationauthors
Yue Y,Yang X,Wei X,Chen J,Fu N,Fu Y,Ba K,Li G,Yao Y,Liang C,Zhang J,Cai X,Wang Mdoi
10.1111/cpr.12035subject
Has Abstractpub_date
2013-06-01 00:00:00pages
320-7issue
3eissn
0960-7722issn
1365-2184journal_volume
46pub_type
杂志文章abstract:OBJECTIVES:The aim of this study was to investigate the role of insulin-like growth factor-1 (IGF-1) and crosstalk between endothelial cells (ECs) and adipose-derived stem cells (ASCs) in the process of angiogenesis. METHODS:A three-dimensional collagen gel used to culture mouse ASCs and mouse ECs in vitro was establi...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12390
更新日期:2017-12-01 00:00:00
abstract:OBJECTIVES:Nonylphenol (NP) is present ubiquitously in both aquatic and terrestrial environments. This compound is considered an important endocrine disruptor and its toxic/oestrogenic activity has been investigated in a number of in vitro and in vivo studies. Human exposure to NP may occur by cutaneous absorption, ing...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.2011.00774.x
更新日期:2011-10-01 00:00:00
abstract:OBJECTIVE:Curcumin (capable of inhibiting angiogenic growth of human umbilical vein endothelial cells [HUVECs]), can be employed in vitro as a model of pathogenesis of corneal neovascularization (CRNV). The aim of this study was to explore regulatory mechanisms of microRNA (miR) levels after curcumin treatment. MATERI...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12289
更新日期:2016-12-01 00:00:00
abstract:OBJECTIVES:The aim of this study was to develop functionalized nanofibres as a simple delivery system for growth factors (GFs) and make nanofibre cell-seeded scaffold implants a one-step intervention. MATERIALS AND METHODS:We have functionalized polycaprolactone (PCL) nanofibres with thrombocytes adherent on them. Imm...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.2011.00737.x
更新日期:2011-04-01 00:00:00
abstract:OBJECTIVES:Osteopetrosis is a rare inherited skeletal disease characterized by increased bone mineral density due to the loss of osteoclast function or differentiation potential. MATERIALS AND METHODS:The study involved a Chinese patient with osteopetrosis (the proband) and her immediate family members and 180 control...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12693
更新日期:2019-11-01 00:00:00
abstract::The R- cell line is a 3T3-like cell line originating from mouse embryos with a homozygous disruption of the type 1 insulin-like growth factor receptor (IGF-IR) genes. Although R- cells cannot grow at all in serum-free medium (SFM) supplemented by several known growth factors, either singly or in combination, they are ...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:
更新日期:1997-06-01 00:00:00
abstract:OBJECTIVES:Small aromatic compounds like flavonoids can intercalate with DNA molecules bringing about conformational changes leading to reduced replication and transcription. Here, we have examined one dietary flavonoid, quercetin (found in many fruit and vegetables), for possible anti-cancer effects, on HeLa cells ori...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12017
更新日期:2013-04-01 00:00:00
abstract:OBJECTIVE:Serum is usually added to growth media when mammalian cells are cultured in vitro to supply the cells with growth factors, hormones, nutrients and trace elements. Defined proteins and metal ions, such as insulin, growth factors, transferrin and sodium selenite, are sometimes also included and can in some case...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.2007.00500.x
更新日期:2008-02-01 00:00:00
abstract::On 31 May 2010, 14 072 567 bone marrow/apheresis donors registered in 44 countries and 426 501 cord blood units banked in 26 countries for public use were available to treat candidates to haemopoietic stem cell transplant lacking a family related compatible donor. Despite these impressive numbers, additional efforts a...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.2010.00720.x
更新日期:2011-04-01 00:00:00
abstract:OBJECTIVES:In humans, non-obstructive azoospermia (NOA) is a major cause of male infertility. However, the aetiology of NOA is largely unknown. Previous studies reported that protein CK2β was abundantly and broadly expressed in spermatogenic cells. Here, we investigate whether protein CK2β participates in spermatogenes...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12726
更新日期:2020-01-01 00:00:00
abstract:OBJECTIVE:Five-year survival rate at early lung tumour was about 70%; however, its early diagnosis rate was still at a low level, so the enhancement of diagnosis level for early lung tumour is the key factor to increase the survival rate. Diagnosis and therapy of early lung tumour are still challenged. METHODS:The mag...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12486
更新日期:2018-12-01 00:00:00
abstract:OBJECTIVES:Oral cancer represents one of the most common malignancies in humans. Its prognosis is still poor, despite the most recent improvements in therapies. An increasing attention is placed on the role of programmed death ligand 1 (PD-L1) in the tumour immunity and its potential function as a marker for tumour pro...
journal_title:Cell proliferation
pub_type: 杂志文章,meta分析
doi:10.1111/cpr.12537
更新日期:2019-03-01 00:00:00
abstract:OBJECTIVES:Beryllium salts (here, beryllium sulphate) can produce a cytostatic effect in some cell types. The basis for this effect may include increased expression of proliferation inhibitors, reduced expression of proliferation promoters, or both. This study sought to determine the role of p53, the tumour-suppressing...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12291
更新日期:2016-12-01 00:00:00
abstract:OBJECTIVES:The present study aimed to investigate overall effect of quercetin on proliferation and osteogenic differentiation of mouse adipose stem cells (mASCs) in vitro. MATERIALS AND METHODS:Mouse adipose stem cells were isolated from subcutaneous fat pads and induced into the osteogenic lineage. Effects of quercet...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12097
更新日期:2014-04-01 00:00:00
abstract::Intra-erythrocytic Plasmodium species can be stained with the DNA binding dye, Hoechst 33342, and the distribution of DNA content determined for parasite populations by flow cytometric measurement of fluorescence. Analysis of this distribution will determine the parasitaemia (percentage of erythrocytes infected), and ...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.1992.tb01452.x
更新日期:1992-09-01 00:00:00
abstract:OBJECTIVES:This study focuses on experimental analysis and corresponding mathematical simulation of in vitro HUVECs (human umbilical vein endothelial cells) proliferation in the presence of various types of drugs. MATERIALS AND METHODS:HUVECs, once seeded in Petri dishes, were expanded to confluence. Temporal profiles...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.2010.00711.x
更新日期:2010-12-01 00:00:00
abstract:OBJECTIVES:Advanced head and neck carcinomas (HNCs) are aggressive tumours, mainly due to hypoxia and a cancer stem cell (CSC) subpopulation. The aim of this study was to simulate tumour growth and behaviour during radiotherapy of three HNC groups (governed by different growth kinetics, hypoxia levels and CSC division ...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12251
更新日期:2016-06-01 00:00:00
abstract:OBJECTIVES:Panax ginseng, a well-known traditional Chinese medicine with multiple pharmacological activities, plays a crucial role in modulating mood disorders. Several recent studies have identified an underlying role of Panax ginseng in the prevention and treatment of depression. However, the cellular and molecular m...
journal_title:Cell proliferation
pub_type: 杂志文章,评审
doi:10.1111/cpr.12696
更新日期:2019-11-01 00:00:00
abstract:OBJECTIVES:Thyroid carcinoma (TC) represents a malignant neoplasm affecting the thyroid. Current treatment strategies include the removal of part of the thyroid; however, this approach is associated with a significant risk of developing hypothyroidism. In order to adequately understand the expression profiles of TNRC6C...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12564
更新日期:2019-05-01 00:00:00
abstract:OBJECTIVES:Topographic cues can modulate morphology and differentiation of mesenchymal stem cells. This study aimed to determine how topographic cues of a novel bilayered poly (lactic-co-glycolic acid) (PLGA) scaffold affect osteogenic/odontogenic differentiation of dental pulp stem cells (DPSCs). METHODS:The surface ...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12676
更新日期:2019-11-01 00:00:00
abstract:OBJECTIVES:Intracellular reactive oxygen species (ROS) induced by receptor activator of NF-kB ligand (RANKL) has been proven to be a critical factor in the development of osteoclasts. This study aimed to prove that schisandrin A (Sch), a novel anti-oxidant compound, is able to suppress osteoclastogenesis and prevent bo...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12882
更新日期:2020-10-01 00:00:00
abstract:OBJECTIVES:We have evaluated the physiological roles of transforming growth factor-beta1 (TGF-beta1) on differentiation, migration, proliferation and anti-apoptosis characteristics of cultured spinal cord-derived neural progenitor cells. METHODS:We have used neural progenitor cells that had been isolated and cultured ...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.2008.00514.x
更新日期:2008-04-01 00:00:00
abstract:OBJECTIVE:Low levels of adiponectin (APN), a biomarker of diabetes mellitus, have been implicated in the poor outcome of intracerebral haemorrhage (ICH). Herein, we aimed to demonstrate the neuroprotective effects of a blood-brain barrier-permeable APN peptide (APNp) on ICH injury in diabetic mice and explore the under...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12759
更新日期:2020-02-01 00:00:00
abstract:OBJECTIVES: The focus of this study was to determine the dedicator of cytokinesis 2 (DOCK2), extracellular signal-regulated kinase 1/2 (ERK1/2), c-Jun N-terminal kinase-1 (JNK) and Akt signals involved in CXCL13-mediated prostate cancer (PCa) cell invasion and proliferation. MATERIALS AND METHODS: Androgen-sensitive...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.2011.00757.x
更新日期:2011-08-01 00:00:00
abstract:OBJECTIVES:Steroid-induced osteonecrosis of the femoral head (ONFH) is a common orthopaedic disease of which early detection remains clinically challenging. Accumulating evidences indicated that circulating microRNAs (miRNAs) plays vital roles in the development of several bone diseases. However, the association betwee...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12418
更新日期:2018-02-01 00:00:00
abstract::The human skin equivalent (HSE) is an in vitro reconstructed model that resembles skin morphologically and biochemically. The HSE is formed by overlaying a fibroblast-populated collagen matrix with a suspension of epidermal cells. Basal keratinocytes attach to the dermal equivalent via a newly formed basement membrane...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.1996.tb00981.x
更新日期:1996-12-01 00:00:00
abstract:OBJECTIVES:Accumulating evidence demonstrated that the long noncoding RNA (lncRNA) HOTAIR (Hox transcript antisense intergenic RNA) plays key role in renal cell carcinoma (RCC) malignancy, while microRNA-124 (miR-124) is a tumour suppressor in RCC. The aim of this work was to assess the biological function of HOTAIR an...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12507
更新日期:2018-12-01 00:00:00
abstract:OBJECTIVES:To investigate the role of hierarchical micro/nanoscale topography of direct metal laser sintering (DMLS) titanium surfaces in osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs), as well as the possible underlying epigenetic mechanism. MATERIALS AND METHODS:Three groups of titanium spe...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12460
更新日期:2018-08-01 00:00:00
abstract:OBJECTIVES:This study investigated effects of reduced serum condition and vascular endothelial growth factor (VEGF) on angiogenic potential of adipose stromal cells (ASCs) in vitro. MATERIALS AND METHODS:Adipose stromal cells were cultured in three different types of medium: (i) F12/DMEM (FD) supplemented with 10% FBS...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12029
更新日期:2013-06-01 00:00:00
abstract:OBJECTIVES:To evaluate the rapid repair potential of adipose-derived stem cells (ADSCs) co-overexpressing VEGF and GDNF on bilateral cavernous nerve injury (BCNI) in rat models. Progressive fibrosis of the penis that occurs shortly after BCNI is a key cause of clinical treatment difficulty of erectile dysfunction (ED)....
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12756
更新日期:2020-02-01 00:00:00