Abstract:
OBJECTIVES:Accumulating evidence demonstrated that the long noncoding RNA (lncRNA) HOTAIR (Hox transcript antisense intergenic RNA) plays key role in renal cell carcinoma (RCC) malignancy, while microRNA-124 (miR-124) is a tumour suppressor in RCC. The aim of this work was to assess the biological function of HOTAIR and to explore underlying mechanism involved in HOTAIR/miR-124/alpha-2, 8-sialyltransferase 4 (ST8SIA4) axis-regulated progression in RCC. MATERIALS AND METHODS:Real-time PCR analyses and western blots were performed to the levels of HOTAIR, miR-124 and ST8SIA4 expression in human RCC tissues and RCC cell lines (ACHN and 786-O). Bioinformatics analysis and dual-luciferase reporter assay were used to illustrate relationship between HOTAIR and miR-124 in RCC. Colony formation assays, EdU assays, Ki67 assays and apoptosis assays were taken to evaluate cell proliferation. Tumour xenograft was created to explore the functions of HOTAIR and ST8SIA4 in tumorigenesis in vivo. Migration assays, invasion assays and cell adhesion assays and were also taken to analyse the carcinoma progression. RESULTS:In this study, HOTAIR level was confirmed to be significantly upregulated in RCC samples and RCC cell lines compared with those in the paired adjacent tissues and normal renal cell line. Overexpression of HOTAIR promoted the capability of proliferation, migration and invasion in RCC cell lines. HOTAIR directly bound to miR-124, while miR-124 mediated the expression of ST8SIA4 in RCC cell lines. ST8SIA4 was upregulated in RCC tissues and RCC cell lines. Ectopic expression of ST8SIA4 modulated the proliferation, migration and invasion of RCC cells. Further results indicated that HOTAIR promoted the proliferation and metastasis as a competing endogenous RNA to regulate ST8SIA4 expression by sponging miR-124 in RCC. CONCLUSIONS:Our results demonstrated that HOTAIR mediated RCC progression in part through miR-124/ST8SIA4 axis, which functioned as a new prognostic biomarker in RCC.
journal_name
Cell Prolifjournal_title
Cell proliferationauthors
Pan Y,Wu Y,Hu J,Shan Y,Ma J,Ma H,Qi X,Jia Ldoi
10.1111/cpr.12507subject
Has Abstractpub_date
2018-12-01 00:00:00pages
e12507issue
6eissn
0960-7722issn
1365-2184journal_volume
51pub_type
杂志文章abstract:OBJECTIVES:Histone deacetylase (HDAC) is an important therapeutic target in cancer. Two of the main anticancer mechanisms of HDAC inhibitors are induction of terminal differentiation and inhibition of cell proliferation. To investigate the role of HDAC in maintenance of self-renewal and cell proliferation, we treated m...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.2009.00633.x
更新日期:2009-12-01 00:00:00
abstract:OBJECTIVES:Panax ginseng, a well-known traditional Chinese medicine with multiple pharmacological activities, plays a crucial role in modulating mood disorders. Several recent studies have identified an underlying role of Panax ginseng in the prevention and treatment of depression. However, the cellular and molecular m...
journal_title:Cell proliferation
pub_type: 杂志文章,评审
doi:10.1111/cpr.12696
更新日期:2019-11-01 00:00:00
abstract:OBJECTIVES:To evaluate the effects of rRNA synthesis inhibition on cell cycle progression and cell population growth according to the RB and p53 status. MATERIAL AND METHODS:RB- and p53-proficient U2OS cells and the RB- and p53-deficient SAOS-2 cells were used, rRNA transcription hindered by actinomycin D, and cell cy...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.2007.00448.x
更新日期:2007-08-01 00:00:00
abstract::Autophagy follows a lysosomal degradation pathway in which a cell digests its own components. It is highly regulated by a limited number of autophagy-related genes (Atg) and the proteins they encode, that are crucial for cells to undergo the process via modulating autophagsome formation. Recently, accumulating evidenc...
journal_title:Cell proliferation
pub_type: 杂志文章,评审
doi:10.1111/cpr.12095
更新日期:2014-04-01 00:00:00
abstract::Laminin-2 (LN-2, alpha2beta1gamma1) is a basement membrane-associated laminin isoform usually considered in the context of muscle and nerve tissues. To test the hypothesis that LN-2 can additionally modulate epithelial cell biology, an analysis of the role of LN-2 in cell adhesion, activation of signalling intermediat...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.2004.00292.x
更新日期:2004-04-01 00:00:00
abstract:OBJECTIVES:We previously reported that Golgi phosphoprotein 3 (GOLPH3) promotes glioma progression by inhibiting EGFR endocytosis and degradation, leading to EGFR accumulation and PI3K-AKT pathway over-activation. In the current study, we examine whether GOLPH3 affects the response of glioma cells to gefitinib, an EGFR...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12636
更新日期:2019-07-01 00:00:00
abstract::Breast cancer has seriously been threatening physical and mental health of women in the world, and its morbidity and mortality also show clearly upward trend in China over time. Through inquiry, we find that survival rate of patients with early-stage breast cancer is significantly higher than those with middle- and la...
journal_title:Cell proliferation
pub_type: 杂志文章,评审
doi:10.1111/cpr.12822
更新日期:2020-07-01 00:00:00
abstract:OBJECTIVES:Nonylphenol (NP) is present ubiquitously in both aquatic and terrestrial environments. This compound is considered an important endocrine disruptor and its toxic/oestrogenic activity has been investigated in a number of in vitro and in vivo studies. Human exposure to NP may occur by cutaneous absorption, ing...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.2011.00774.x
更新日期:2011-10-01 00:00:00
abstract:OBJECTIVES:Based on in vivo studies, low-intensity pulsed ultrasound (LIPUS) stimulation has been widely used in the clinic for advancing bone growth during healing of non-union alignment, fractures and other osseous defects. In this study, we have investigated osteogenic differentiation of adipose stem cells (ASCs) re...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12035
更新日期:2013-06-01 00:00:00
abstract:OBJECTIVES:Vascular disorders are associated with phenotypical switching of vascular smooth muscle cells (VSMCs). We investigated the effect of bone morphogenetic protein (BMP)-2 in controlling VSMC phenotype and vascular disorder progression. Lysine (K)-specific demethylase 1A (KDM1A) has been identified to target BMP...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12711
更新日期:2020-01-01 00:00:00
abstract:OBJECTIVES:Small aromatic compounds like flavonoids can intercalate with DNA molecules bringing about conformational changes leading to reduced replication and transcription. Here, we have examined one dietary flavonoid, quercetin (found in many fruit and vegetables), for possible anti-cancer effects, on HeLa cells ori...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12017
更新日期:2013-04-01 00:00:00
abstract:OBJECTIVE:It is not known whether or not epithelial progenitors of the pyloric antrum are involved in gastric carcinogenesis. Normally, these progenitors give rise to two main cell lineages: pit and gland mucous cells. This study was designed to examine the changes that occur in pyloric antral mucous cell lineages and ...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.2008.00562.x
更新日期:2008-12-01 00:00:00
abstract:OBJECTIVES:Translational research using adult stem cells derived from various tissues has been highlighted in cell-based therapy. However, there are many limitations to using conventional culture systems of adult stem cells for clinically applicability, including limited combinations of cytokines and use of nutrients d...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.2010.00693.x
更新日期:2010-10-01 00:00:00
abstract:OBJECTIVE:Serum is usually added to growth media when mammalian cells are cultured in vitro to supply the cells with growth factors, hormones, nutrients and trace elements. Defined proteins and metal ions, such as insulin, growth factors, transferrin and sodium selenite, are sometimes also included and can in some case...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.2007.00500.x
更新日期:2008-02-01 00:00:00
abstract::We have developed a mathematical model based on proliferation and infiltration of neoplastic cells that allows predictions to be made concerning the life expectancies following various extents of surgical resection of gliomas of all grades of malignancy. The key model parameters are the growth rate and the diffusion r...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.1996.tb01580.x
更新日期:1996-06-01 00:00:00
abstract::Reports on the p53-related cell cycle and apoptotic responses of EBV-transformed lymphoblastoid cell lines to DNA damage have led to some confusion. This may be due to differences in the nature of the specific p53 mutations under examination, but it can also be partly attributed to methodological and analytical proble...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:
更新日期:1999-02-01 00:00:00
abstract:OBJECTIVES:High mobility group protein2 (HMGA2) and epithelial-to-mesenchymal transition are both related to progress of bladder cancer, however, the relationship between HMGA2, E-cadherin and vimentin in bladder cancer is not yet known. Thus, this study has examined expression of HMGA2, E-cadherin and vimentin in blad...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12096
更新日期:2014-04-01 00:00:00
abstract:OBJECTIVE:MicroRNAs (miRNAs) are negative regulators of gene expression that play important roles in cell processes such as proliferation, development and differentiation. Recently, it has been reported that miRNAs are related to development of carcinogenesis. The aim of this study was to identify miRNAs associated wit...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.2010.00717.x
更新日期:2011-02-01 00:00:00
abstract:OBJECTIVE:Spinal cord tumours are highly malignant and often lead to paralysis and death due to their infiltrative nature, high recurrence rate and limited treatment options. In this study, we measured antitumour efficacy of the Salmonella typhimurium A1-R tumour-targeting bacterium strain, administered systemically or...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.2009.00652.x
更新日期:2010-02-01 00:00:00
abstract::We previously found that the stable overexpression of oestrogen receptor-alpha in the human endothelial cell line ECV304* inhibits its growth in vitro, and that this inhibition is possibly mediated through a down-regulation of the vasoactive agents endothelin-1 and vascular endothelial growth factor. Here we show an i...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1046/j.0960-7722.2001.00207.x
更新日期:2001-08-01 00:00:00
abstract:OBJECTIVES:Intracellular reactive oxygen species (ROS) induced by receptor activator of NF-kB ligand (RANKL) has been proven to be a critical factor in the development of osteoclasts. This study aimed to prove that schisandrin A (Sch), a novel anti-oxidant compound, is able to suppress osteoclastogenesis and prevent bo...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12882
更新日期:2020-10-01 00:00:00
abstract:OBJECTIVES:Oestrogen is known to inhibit osteoclastogenesis, and numerous studies have identified it as an autophagic activator. To date, the role of oestrogen in the autophagy of osteoclast precursors (OCPs) during osteoclastogenesis remains unclear. This study aimed to determine the effect of autophagy regulated by t...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12789
更新日期:2020-04-01 00:00:00
abstract:OBJECTIVES:This study was designed to investigate the role of circ_0078767/miR-330-3p/RASSF1A in non-small-cell lung cancer (NSCLC). Bioinformatic analysis was performed to screen for the differentially expressed genes in NSCLC tissues from adjacent lung tissues. MATERIALS AND METHODS:qRT-PCR was used to detect the RN...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12548
更新日期:2019-03-01 00:00:00
abstract::The effect of various GnRH analogues, and their conjugates on proliferation, clonogenicity and cell cycle phase distribution of MCF-7 and Ishikawa human cancer cell lines was studied. GnRH-III, a sea lamprey GnRH analogue reduced cell proliferation by 35% and clonogenicity by 55%. Structural modifications either decre...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1046/j.1365-2184.2000.00180.x
更新日期:2000-10-01 00:00:00
abstract:OBJECTIVES:Induced pluripotent stem cell (iPSC)-derived cardiomyocytes are a promising source of cells for regenerative heart disease therapies, but progress towards their use has been limited by their low differentiation efficiency and high cellular heterogeneity. Previous studies have demonstrated expression of adren...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12310
更新日期:2017-02-01 00:00:00
abstract:OBJECTIVES:The aim of this study was to obtain antitumour molecules targeting to activate PKM2 through adequate computational methods combined with biological activity experiments. METHODS:The structure-based virtual screening was utilized to screen effective activator targeting PKM2 from ZINC database. Molecular dyna...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12509
更新日期:2018-12-01 00:00:00
abstract:OBJECTIVES:Restoring a functional beta-cell mass is a fundamental goal in treating diabetes. A complex signalling pathway network coordinates the regulation of beta-cell proliferation, although a role for ERK5 in this network has not been reported. This question was addressed in this study. MATERIALS AND METHODS:We st...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12410
更新日期:2018-06-01 00:00:00
abstract::In the past few years, the paramount role of cancer stem cells (CSCs), in terms of cancer initiation, proliferation, metastasis, invasion and chemoresistance, has been revealed by accumulating studies. However, this level of cellular plasticity cannot be entirely explained by genetic mutations. Research on epigenetic ...
journal_title:Cell proliferation
pub_type: 杂志文章,评审
doi:10.1111/cpr.12963
更新日期:2020-12-13 00:00:00
abstract::Studies to examine the percentages of proliferating plasma cells (PPC) in the Harderian gland (HG) were carried out in chicks between 5 and 12 weeks of age. Two methods, 5-bromo-2'-deoxyuridine (BrdUrd) incorporation into DNA and flow cytometric analysis of propidium iodide (PI) stained cells, were employed in control...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.1992.tb01444.x
更新日期:1992-07-01 00:00:00
abstract:OBJECTIVES:During human pregnancy, the endothelial cells of the uterine spiral arteries (SPA) are extensively replaced by a subtype of placental trophoblasts, endovascular extravillous trophoblasts (enEVTs), thus establishing a placental-maternal circulation. On this pathway, foetus-derived placental villi and enEVTs b...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12802
更新日期:2020-05-01 00:00:00