Abstract:
:Studies to examine the percentages of proliferating plasma cells (PPC) in the Harderian gland (HG) were carried out in chicks between 5 and 12 weeks of age. Two methods, 5-bromo-2'-deoxyuridine (BrdUrd) incorporation into DNA and flow cytometric analysis of propidium iodide (PI) stained cells, were employed in control and emetine dihydrochloride treated birds. Flow cytometric analysis of PI stained cells showed the percentages of plasma cells in S phase were highest between 6 and 8 weeks of age. After this period of time, the number of S phase plasma cells decreased and remained low through 12 weeks of age. The lowest percentages of plasma cells in G0 + G1 were found at 6 and 8 weeks of age, and all ages had equal percentages of plasma cells in G2 + M phase. After administration of the protein synthesis inhibitor emetine dihydrochloride a common pattern of plasma cell depletion and repopulation in the HG was observed. At 3 and 5 days post-treatment the plasma cell population in the gland decreased and by 7 days post-treatment repopulation of the gland with plasma cells had taken place. Anti-BrdUrd staining of frozen sections revealed that the number of PPC were decreased at 3 days after emetine treatment but were as high as, or higher than, controls at 5 and 7 days post-treatment. Flow cytometric analysis indicated that some birds were more severely affected by emetine. Namely, the percentages of plasma cells in S phase were lower at 3 and 5 days post-treatment. Even though most birds were severely affected by emetine treatment during the experiments, they possessed a cell population with the proliferative capacity to quickly repopulate the HG by 7 days post-emetine treatment.
journal_name
Cell Prolifjournal_title
Cell proliferationauthors
Savage ML,Oláh I,Scott TRdoi
10.1111/j.1365-2184.1992.tb01444.xsubject
Has Abstractpub_date
1992-07-01 00:00:00pages
337-44issue
4eissn
0960-7722issn
1365-2184journal_volume
25pub_type
杂志文章abstract:OBJECTIVE:Low levels of adiponectin (APN), a biomarker of diabetes mellitus, have been implicated in the poor outcome of intracerebral haemorrhage (ICH). Herein, we aimed to demonstrate the neuroprotective effects of a blood-brain barrier-permeable APN peptide (APNp) on ICH injury in diabetic mice and explore the under...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12759
更新日期:2020-02-01 00:00:00
abstract:OBJECTIVES:Translational research using adult stem cells derived from various tissues has been highlighted in cell-based therapy. However, there are many limitations to using conventional culture systems of adult stem cells for clinically applicability, including limited combinations of cytokines and use of nutrients d...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.2010.00693.x
更新日期:2010-10-01 00:00:00
abstract:OBJECTIVE:MicroRNAs (miRNAs) are negative regulators of gene expression that play important roles in cell processes such as proliferation, development and differentiation. Recently, it has been reported that miRNAs are related to development of carcinogenesis. The aim of this study was to identify miRNAs associated wit...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.2010.00717.x
更新日期:2011-02-01 00:00:00
abstract:OBJECTIVES:Temozolomide (TMZ) is one of the most commonly used clinical drugs for glioblastoma (GBM) treatment, but its drug sensitivity needs to be improved. Gamabufotalin (CS-6), the primary component of the traditional Chinese medicine "ChanSu," was shown to have strong anti-cancer activity. However, more efforts sh...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12732
更新日期:2020-01-01 00:00:00
abstract:OBJECTIVES:Induced pluripotent stem cell (iPSC)-derived cardiomyocytes are a promising source of cells for regenerative heart disease therapies, but progress towards their use has been limited by their low differentiation efficiency and high cellular heterogeneity. Previous studies have demonstrated expression of adren...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12310
更新日期:2017-02-01 00:00:00
abstract::A model of intestinal crypt organization is suggested based on the assumption that stem cells have a finite replicative life span. The model assumes the existence in a crypt of a quiescent ('deep') stem cell and a few more actively cycling ('proximate') stem cells. Monte Carlo computer simulation of published intestin...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.2006.00395.x
更新日期:2006-10-01 00:00:00
abstract:OBJECTIVES:Bone marrow edema is a universal manifestation of rheumatoid arthritis (RA), and its pathological essence is a bone marrow lesion (BML) formed by various bone marrow (BM) immune cells. Neutrophils play an important role in inflammatory arthritis, but the role and mechanism of neutrophils in BML are not clear...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12824
更新日期:2020-07-01 00:00:00
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journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12548
更新日期:2019-03-01 00:00:00
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journal_title:Cell proliferation
pub_type: 杂志文章
doi:
更新日期:1999-02-01 00:00:00
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journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1046/j.1365-2184.2001.00222.x
更新日期:2001-12-01 00:00:00
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journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.2004.00308.x
更新日期:2004-06-01 00:00:00
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journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12035
更新日期:2013-06-01 00:00:00
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journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12443
更新日期:2018-08-01 00:00:00
abstract::This study focuses on analysis of in vitro cultures of chondrocytes from ovine articular cartilage. Isolated cells were seeded in Petri dishes, then expanded to confluence and phenotypically characterized by flow cytometry. The sigmoidal temporal profile of total counts was obtained by classic haemocytometry and corre...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.2010.00676.x
更新日期:2010-06-01 00:00:00
abstract::Studies on growth regulation in vitro to a large extent rely on comparison of growth curves. However, these do not discriminate between the relative contributions of the mitotic rate and the apoptotic rate to the net growth rate. In the present study, differential effects of 17beta-oestradiol (E2, 10(-8) M) and/or tam...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1046/j.1365-2184.1999.3250289.x
更新日期:1999-10-01 00:00:00
abstract:OBJECTIVES:BCL2 family proteins have been widely studied over the past decade due to their essential roles in apoptosis, oncogenesis and anti-cancer therapy. However, the similarities and differences in the spatial pattern of the BCL2 gene family within the context of chromatin have not been well characterized. We soug...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12826
更新日期:2020-06-01 00:00:00
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journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12693
更新日期:2019-11-01 00:00:00
abstract::Atherosclerosis is an inflammatory-fibroproliferative response of the arterial wall involving a complex set of interconnected events where cell proliferation (lymphomonocytes, and endothelial and smooth-muscle cells) and substantial perturbations of intracellular cholesterol metabolism are considered to be among the m...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1046/j.1365-2184.2002.00231.x
更新日期:2002-06-01 00:00:00
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journal_title:Cell proliferation
pub_type: 杂志文章,评审
doi:10.1111/cpr.12915
更新日期:2020-11-01 00:00:00
abstract:OBJECTIVES:The present study aimed to investigate overall effect of quercetin on proliferation and osteogenic differentiation of mouse adipose stem cells (mASCs) in vitro. MATERIALS AND METHODS:Mouse adipose stem cells were isolated from subcutaneous fat pads and induced into the osteogenic lineage. Effects of quercet...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12097
更新日期:2014-04-01 00:00:00
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journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1046/j.1365-2184.2003.00279.x
更新日期:2003-08-01 00:00:00
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journal_title:Cell proliferation
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更新日期:2017-12-01 00:00:00
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journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12346
更新日期:2017-08-01 00:00:00
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journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.2009.00652.x
更新日期:2010-02-01 00:00:00
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journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.1996.tb00981.x
更新日期:1996-12-01 00:00:00
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journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.2010.00684.x
更新日期:2010-08-01 00:00:00
abstract::Chronic kidney disease (CKD) is a severe health problem worldwide, and vascular calcification (VC) contributes substantially to the cardiovascular morbidity and high mortality of CKD. CKD is often accompanied by a variety of pathophysiological states, such as inflammation, oxidative stress, hyperglycaemia, hyperparath...
journal_title:Cell proliferation
pub_type: 杂志文章,评审
doi:10.1111/cpr.12980
更新日期:2021-01-27 00:00:00
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journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.2007.00448.x
更新日期:2007-08-01 00:00:00
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journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12917
更新日期:2020-11-01 00:00:00
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journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12087
更新日期:2014-02-01 00:00:00