Abstract:
OBJECTIVES:Restoring a functional beta-cell mass is a fundamental goal in treating diabetes. A complex signalling pathway network coordinates the regulation of beta-cell proliferation, although a role for ERK5 in this network has not been reported. This question was addressed in this study. MATERIALS AND METHODS:We studied the activation of extracellular-signal-regulated kinase 5 (ERK5) in pregnant mice, a well-known mouse model of increased beta-cell proliferation. A specific inhibitor of ERK5 activation, BIX02189, was intraperitoneally injected into the pregnant mice to suppress ERK5 signalling. Beta-cell proliferation was determined by quantification of Ki-67+ beta cells. Beta-cell apoptosis was determined by TUNEL assay. The extent of beta-cell proliferation was determined by beta-cell mass. The alteration of ERK5 activation and CyclinD1 levels in purified mouse islets was examined by Western blotting. RESULTS:Extracellular-signal-regulated kinase 5 phosphorylation, which represents ERK5 activation, was significantly upregulated in islets from pregnant mice. Suppression of ERK5 activation by BIX02189 in pregnant mice significantly reduced beta-cell proliferation, without affecting beta-cell apoptosis, resulting in increases in random blood glucose levels and impairment of glucose response of the mice. ERK5 seemed to activate CyclinD1 to promote gestational beta-cell proliferation. CONCLUSIONS:Extracellular-signal-regulated kinase 5 plays an essential role in the gestational augmentation of beta-cell proliferation. ERK5 may be a promising target for increasing beta-cell mass in diabetes patients.
journal_name
Cell Prolifjournal_title
Cell proliferationauthors
Chen C,Wu S,Lin X,Wu D,Fischbach S,Xiao Xdoi
10.1111/cpr.12410subject
Has Abstractpub_date
2018-06-01 00:00:00pages
e12410issue
3eissn
0960-7722issn
1365-2184journal_volume
51pub_type
杂志文章abstract::The present study was designed to analyse the effect of red blood cells on T-cell proliferation and expansion. A comparative study was done in peripheral blood cell cultures stimulated with phytohemagglutinin, with or without red blood cells. The presence of red blood cells had a consistent enhancing effect on T lymph...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1046/j.1365-2184.2001.00222.x
更新日期:2001-12-01 00:00:00
abstract:OBJECTIVES:Hyperinsulinemia is a risk factor for pancreatic cancer, but the function of insulin in carcinogenesis is unclear, so this study aimed to elucidate the carcinogenic effects of insulin and the synergistic effect with the KRAS mutation in the early stage of pancreatic cancer. MATERIALS AND METHODS:A pair of i...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12575
更新日期:2019-05-01 00:00:00
abstract:OBJECTIVE:Serum is usually added to growth media when mammalian cells are cultured in vitro to supply the cells with growth factors, hormones, nutrients and trace elements. Defined proteins and metal ions, such as insulin, growth factors, transferrin and sodium selenite, are sometimes also included and can in some case...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.2007.00500.x
更新日期:2008-02-01 00:00:00
abstract:OBJECTIVE:Five-year survival rate at early lung tumour was about 70%; however, its early diagnosis rate was still at a low level, so the enhancement of diagnosis level for early lung tumour is the key factor to increase the survival rate. Diagnosis and therapy of early lung tumour are still challenged. METHODS:The mag...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12486
更新日期:2018-12-01 00:00:00
abstract::Stem cells are regenerating medicine. Advances in stem cell biology, and bone marrow-derived mesenchymal stem cells in particular, are demonstrating that many clinical options once thought to be science fiction may be attainable as fact. The extra- and intra-cellular signalling used by stem cells as they differentiate...
journal_title:Cell proliferation
pub_type: 杂志文章,评审
doi:10.1111/j.1365-2184.2004.00303.x
更新日期:2004-02-01 00:00:00
abstract:OBJECTIVES:Generally, the secretory forms of FGF are known to regulate cell proliferation, differentiation and morphogenesis by binding to the extracellular domain of cell surface receptors. Intracellular FGFs (FGF11-14) are expressed principally in the nervous system. FGF13 is a microtubule-stabilizing protein that re...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12200
更新日期:2015-10-01 00:00:00
abstract:OBJECTIVES:It has been widely reported that maternal diabetes impairs oocyte quality. However, the responsible mechanisms remain to be explored. In the present study, we focused on whether SIRT3-GSK3β pathway mediates the meiotic defects in oocytes from diabetic mice. MATERIALS AND METHODS:GSK3β functions in mouse ooc...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12940
更新日期:2021-01-01 00:00:00
abstract:OBJECTIVES:Often, non-small cell lung cancers (NSCLC) respond only poorly to the tyrosine kinase inhibitor (TKI) gefitinib, which targets the epidermal growth factor receptor (EGFR), these poor responders EGFRs lacking activating mutations. In this study, we have attempted to improve TKI response of NSCLC cell lines (A...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12040
更新日期:2013-08-01 00:00:00
abstract::In vitro cultured Ehrlich ascites tumour (EAT) cells were used because of the ease of their manipulation under different levels of hypoxia. They were used to clarify further the complex mechanism of oxygen-dependent cell proliferation. On reducing the oxygen concentration from 20% to lower levels (1-7%) an increase in...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.1992.tb01392.x
更新日期:1992-05-01 00:00:00
abstract:OBJECTIVES:Limbal stem cells (LSC) are self-renewing, highly proliferative cells in vitro, which express a set of specific markers and in vivo have the capacity to reconstruct the entire corneal epithelium in cases of ocular surface injury. Currently, LSC transplantation is a commonly used procedure in patients with ei...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.2009.00623.x
更新日期:2009-10-01 00:00:00
abstract:OBJECTIVES:Ubiquitin specific protease 32 (USP32) is a highly conserved but uncharacterized gene, which has been reported to be associated with growth of breast cancer cells. However, the role of USP32 in human small cell lung cancer (SCLC) has not been uncovered. The aim of this study was to investigate and evaluate t...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12343
更新日期:2017-08-01 00:00:00
abstract:OBJECTIVES:Glucagon-like peptide 2 (GLP2) is involved in the regulation of energy absorption and metabolism. Despite the importance of the GLP2 signalling mechanisms on osteoclast, little has been studied on how GLP2 works during osteoclastogenesis. MATERIALS AND METHODS:RAW264.7 cells were infected with rLV-Green-GLP...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12443
更新日期:2018-08-01 00:00:00
abstract:OBJECTIVES:SAV1 is a human homologue of Salvador that contains two protein-protein interaction modules known as WW domains and acts as a scaffolding protein. SAV1 participates in the development of diverse types of cancer. We aimed to investigate the role of SAV1 in human colorectal cancer. MATERIALS AND METHODS:Human...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12351
更新日期:2017-08-01 00:00:00
abstract::The microenvironmental changes in the bone marrow, spleen and liver during progression of the transplantable promyelocytic leukaemia in the Brown Norwegian rat (BNML) have been studied. We used flow cytometry to estimate cellular hypoxia and proliferation based on in vivo pulse-labelling with a mixture of 2-nitroimida...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1046/j.1365-2184.2000.00183.x
更新日期:2000-12-01 00:00:00
abstract:OBJECTIVES:Osteosarcoma is the most commonly diagnosed primary malignancy of bone and its overall survival rate is still very low. The molecular mechanisms underlying the progression of osteosarcoma have not been clearly illuminated. Heat shock transcription factor 1 (HSF1) is a key regulator of the heat shock response...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12346
更新日期:2017-08-01 00:00:00
abstract:OBJECTIVES:Induced pluripotent stem cell (iPSC)-derived cardiomyocytes are a promising source of cells for regenerative heart disease therapies, but progress towards their use has been limited by their low differentiation efficiency and high cellular heterogeneity. Previous studies have demonstrated expression of adren...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12310
更新日期:2017-02-01 00:00:00
abstract:OBJECTIVES:Skin-derived precursors are recognized to be a potentially autologous and accessible source of neural precursor cells for drug screening or cell-based treatments, in many neurological disorders. Thus, it is necessary to investigate appropriate methods for cryopreservation of such human skin-derived precursor...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.2011.00803.x
更新日期:2012-04-01 00:00:00
abstract:OBJECTIVES:Stiffness of bone tissue differs response to its physiological or pathological status, such as osteoporosis or osteosclerosis. Consequently, the function of cells residing in bone tissue including osteoblasts (OBs), osteoclasts and osteocytes will be affected. However, to the best of our knowledge, the detai...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12338
更新日期:2017-06-01 00:00:00
abstract::Reports on the p53-related cell cycle and apoptotic responses of EBV-transformed lymphoblastoid cell lines to DNA damage have led to some confusion. This may be due to differences in the nature of the specific p53 mutations under examination, but it can also be partly attributed to methodological and analytical proble...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:
更新日期:1999-02-01 00:00:00
abstract::The expression of the 170-kDa (alpha) and the 180-kDa (beta) isoforms of DNA topoisomerase II (topo II) was investigated with two specific monoclonal antibodies (MoAbs) in human peripheral blood lymphocytes (PBL), before and after phytohaemoagglutinin (PHA) stimulation. Binding of each MoAb was detected by indirect im...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.1994.tb01423.x
更新日期:1994-05-01 00:00:00
abstract::Telomere length plays an important role in regulating the proliferative capacity of cells, and serves as a marker for cell cycle history and also for their remaining replicative potential. Mesenchymal stromal cells (MSC) are known to be a significant cell source for therapeutic intervention and tissue engineering. To ...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.2006.00397.x
更新日期:2006-12-01 00:00:00
abstract:OBJECTIVES:The transfer of melanosomes from melanocytes to neighbouring keratinocytes is critical to protect the skin from the deleterious effects of ultraviolet A (UVA) and ultraviolet B (UVB) irradiation; however, the initial factor(s) that stimulates melanosome transfer remains unclear. In this study, we investigate...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12372
更新日期:2017-12-01 00:00:00
abstract:OBJECTIVES:BCL2 family proteins have been widely studied over the past decade due to their essential roles in apoptosis, oncogenesis and anti-cancer therapy. However, the similarities and differences in the spatial pattern of the BCL2 gene family within the context of chromatin have not been well characterized. We soug...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12826
更新日期:2020-06-01 00:00:00
abstract:OBJECTIVE:Erythropoietin has been shown to have a protective effect in certain models of ischaemia-reperfusion, and in some cases the protection has been correlated with activation of signalling pathways known to play a role in cell survival and proliferation. We have studied whether erythropoietin would overcome direc...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.2009.00617.x
更新日期:2009-08-01 00:00:00
abstract:OBJECTIVE:Accumulating data show that dysregulation of long noncoding RNAs (lncRNAs) acts a critical role in a variety of malignancies. Among these lncRNAs, small nucleolar RNA host genes (SNHGs) are associated with tumour growth and progression. But, the molecular mechanisms by which SNHG4 contributes to osteosarcoma ...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12515
更新日期:2018-12-01 00:00:00
abstract::Estimates of the clonogen content (number of microcolony-forming cells) of murine intestinal crypts using microcolony assays show an apparent dependence on the radiation dose used in the assay of clonogen content. Crypt radiation survival curves often show increased curvature beyond that expected on the basis of the c...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1046/j.1365-2184.2003.00279.x
更新日期:2003-08-01 00:00:00
abstract:OBJECTIVE:Low levels of adiponectin (APN), a biomarker of diabetes mellitus, have been implicated in the poor outcome of intracerebral haemorrhage (ICH). Herein, we aimed to demonstrate the neuroprotective effects of a blood-brain barrier-permeable APN peptide (APNp) on ICH injury in diabetic mice and explore the under...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12759
更新日期:2020-02-01 00:00:00
abstract::Two groups of male rats were treated orally for 60 days with ebrotidine and cimetidine, used as reference standard, respectively. The dose used of both drugs was 500 mg/kg. A third group, used as control, received 10 ml/kg of an aqueous agar suspension. After receiving the last dose, the animals were killed by inhalat...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.1995.tb00080.x
更新日期:1995-07-01 00:00:00
abstract:OBJECTIVES:Topographic cues can modulate morphology and differentiation of mesenchymal stem cells. This study aimed to determine how topographic cues of a novel bilayered poly (lactic-co-glycolic acid) (PLGA) scaffold affect osteogenic/odontogenic differentiation of dental pulp stem cells (DPSCs). METHODS:The surface ...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12676
更新日期:2019-11-01 00:00:00
abstract:OBJECTIVES:We have evaluated the physiological roles of transforming growth factor-beta1 (TGF-beta1) on differentiation, migration, proliferation and anti-apoptosis characteristics of cultured spinal cord-derived neural progenitor cells. METHODS:We have used neural progenitor cells that had been isolated and cultured ...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.2008.00514.x
更新日期:2008-04-01 00:00:00