Abstract:
:The present study was designed to analyse the effect of red blood cells on T-cell proliferation and expansion. A comparative study was done in peripheral blood cell cultures stimulated with phytohemagglutinin, with or without red blood cells. The presence of red blood cells had a consistent enhancing effect on T lymphocyte proliferation, as determined by an increase in both the mitotic index and thymidine uptake. Phenotypic characterization of T cell blasts by flow cytometry revealed that, in the presence of red blood cells, expanding cells were preferentially CD8+ cells. Accordingly, proliferation of CD8+ lymphocytes from two patients with CD8+ hyperlymphocytosis was dependent on the presence of red blood cells. In contrast, proliferation of CD4+ lymphocytes from two patients with CD4+ hyperlymphocytosis was strongly inhibited by the presence of red blood cells. This is the first reported evidence that human red blood cells have an enhancing effect on the expansion of CD8+ lymphocytes in vitro.
journal_name
Cell Prolifjournal_title
Cell proliferationauthors
Porto B,Fonseca AM,Godinho I,Arosa FA,Porto Gdoi
10.1046/j.1365-2184.2001.00222.xsubject
Has Abstractpub_date
2001-12-01 00:00:00pages
359-67issue
6eissn
0960-7722issn
1365-2184pii
222journal_volume
34pub_type
杂志文章abstract::Stem cells are regenerating medicine. Advances in stem cell biology, and bone marrow-derived mesenchymal stem cells in particular, are demonstrating that many clinical options once thought to be science fiction may be attainable as fact. The extra- and intra-cellular signalling used by stem cells as they differentiate...
journal_title:Cell proliferation
pub_type: 杂志文章,评审
doi:10.1111/j.1365-2184.2004.00303.x
更新日期:2004-02-01 00:00:00
abstract:OBJECTIVES:Limbal stem cells (LSC) are self-renewing, highly proliferative cells in vitro, which express a set of specific markers and in vivo have the capacity to reconstruct the entire corneal epithelium in cases of ocular surface injury. Currently, LSC transplantation is a commonly used procedure in patients with ei...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.2009.00623.x
更新日期:2009-10-01 00:00:00
abstract:OBJECTIVES:Vascular disorders are associated with phenotypical switching of vascular smooth muscle cells (VSMCs). We investigated the effect of bone morphogenetic protein (BMP)-2 in controlling VSMC phenotype and vascular disorder progression. Lysine (K)-specific demethylase 1A (KDM1A) has been identified to target BMP...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12711
更新日期:2020-01-01 00:00:00
abstract:OBJECTIVES:Long non-coding RNAs have identified to involve into the tumour cell proliferation, apoptosis and metastasis. We previously found that up-regulated LncRNA-SNHG7 (SNHG7) positively correlated to the Fas apoptosis inhibitory molecule 2 (FAIM2) in lung cancer cells with unclear mechanism. METHODS:Non-small cel...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12406
更新日期:2018-02-01 00:00:00
abstract:OBJECTIVES:Osteosarcoma is the most commonly diagnosed primary malignancy of bone and its overall survival rate is still very low. The molecular mechanisms underlying the progression of osteosarcoma have not been clearly illuminated. Heat shock transcription factor 1 (HSF1) is a key regulator of the heat shock response...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12346
更新日期:2017-08-01 00:00:00
abstract:OBJECTIVES:Silicate bioactive glass (BG) has been widely demonstrated to stimulate both of the hard and soft tissue regeneration, in which ion products released from BG play important roles. However, the mechanism by which ion products act on cells on cells is unclear. MATERIALS AND METHODS:Human umbilical vein endoth...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12906
更新日期:2020-11-01 00:00:00
abstract::Estimates of the clonogen content (number of microcolony-forming cells) of murine intestinal crypts using microcolony assays show an apparent dependence on the radiation dose used in the assay of clonogen content. Crypt radiation survival curves often show increased curvature beyond that expected on the basis of the c...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1046/j.1365-2184.2003.00279.x
更新日期:2003-08-01 00:00:00
abstract::Neural stem cells (NSCs) are early precursors of neuronal and glial cells. NSCs are capable of generating identical progeny through virtually unlimited numbers of cell divisions (cell proliferation), producing daughter cells committed to differentiation. Nuclear factor kappa B (NF-kappaB) is an inducible, ubiquitous t...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.2006.00403.x
更新日期:2006-12-01 00:00:00
abstract:OBJECTIVES:Oral cancer represents one of the most common malignancies in humans. Its prognosis is still poor, despite the most recent improvements in therapies. An increasing attention is placed on the role of programmed death ligand 1 (PD-L1) in the tumour immunity and its potential function as a marker for tumour pro...
journal_title:Cell proliferation
pub_type: 杂志文章,meta分析
doi:10.1111/cpr.12537
更新日期:2019-03-01 00:00:00
abstract:OBJECTIVES:This study is to explore the role of Notch signalling during the regeneration of rat tracheal epithelium after injury induced by 5-fluorouracil (5-FU). MATERIALS AND METHODS:We developed an ex vivo model of rat tracheal epithelial regeneration using 5-FU to induce injury. Expression levels of members of the...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.2008.00569.x
更新日期:2009-02-01 00:00:00
abstract:OBJECTIVES:Pre-eclampsia is a leading cause of morbidity and mortality during pregnancy. Although the two forms of this disorder, early- (EOPE) and late-onset of pre-eclampsia (LOPE) are different, the underlying pathology remains elusive. We aim to unravel the difference and to identify novel biomarkers for EOPE and L...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12968
更新日期:2020-12-17 00:00:00
abstract::Rabbit embryo-fetal fluid (EFF) contains regulatory factors of cell proliferation which increase the duration of the cell cycle, induce a quiescent status in some cells and lead up to cell death in others. The objective of this study was to demonstrate which of the two processes, namely necrosis or apoptosis, was resp...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.1993.tb00013.x
更新日期:1993-03-01 00:00:00
abstract::Cell proliferation was investigated in human tumour xenografts using bromodeoxyuridine (BrdUrd) labelling, evaluated either by flow cytometry or in tissue sections, and also using the proliferation marker Ki-67. BrdUrd labelling was found to increase when cryostat tumour sections were digested with an enzymic solution...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.1991.tb01147.x
更新日期:1991-03-01 00:00:00
abstract::The human skin equivalent (HSE) is an in vitro reconstructed model that resembles skin morphologically and biochemically. The HSE is formed by overlaying a fibroblast-populated collagen matrix with a suspension of epidermal cells. Basal keratinocytes attach to the dermal equivalent via a newly formed basement membrane...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.1996.tb00981.x
更新日期:1996-12-01 00:00:00
abstract::We previously found that the stable overexpression of oestrogen receptor-alpha in the human endothelial cell line ECV304* inhibits its growth in vitro, and that this inhibition is possibly mediated through a down-regulation of the vasoactive agents endothelin-1 and vascular endothelial growth factor. Here we show an i...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1046/j.0960-7722.2001.00207.x
更新日期:2001-08-01 00:00:00
abstract:OBJECTIVES:We previously reported that Golgi phosphoprotein 3 (GOLPH3) promotes glioma progression by inhibiting EGFR endocytosis and degradation, leading to EGFR accumulation and PI3K-AKT pathway over-activation. In the current study, we examine whether GOLPH3 affects the response of glioma cells to gefitinib, an EGFR...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12636
更新日期:2019-07-01 00:00:00
abstract:OBJECTIVES:This study aims to explore the roles of N-myc and caspase-8 in TRAIL-resistant IMR-32 cells which exhibit MYCN oncogene amplification and lack caspase-8 expression. MATERIALS AND METHODS:We established N-myc-downregulated IMR-32 cells using shRNA lentiviral particles targeting N-myc and examined the effect ...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12577
更新日期:2019-05-01 00:00:00
abstract:OBJECTIVES:The main objective of this paper was to investigate whether the oxygen consumption rate (OCR) of cells entrapped in alginate hydrogels depends on presence of soluble factors present in foetal bovine serum (FBS). MATERIALS AND METHODS:Pericellular oxygen concentrations were measured using a photochemical oxy...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12178
更新日期:2015-06-01 00:00:00
abstract:OBJECTIVES:In order to provide a more comprehensive understanding of the effects of SARS-CoV-2 on oral health and possible saliva transmission, we performed RNA-seq profiles analysis from public databases and also a questionnaire survey on oral-related symptoms of COVID-19 patients. MATERIALS AND METHODS:To analyse AC...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12923
更新日期:2020-12-01 00:00:00
abstract::The microenvironmental changes in the bone marrow, spleen and liver during progression of the transplantable promyelocytic leukaemia in the Brown Norwegian rat (BNML) have been studied. We used flow cytometry to estimate cellular hypoxia and proliferation based on in vivo pulse-labelling with a mixture of 2-nitroimida...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1046/j.1365-2184.2000.00183.x
更新日期:2000-12-01 00:00:00
abstract:OBJECTIVES:Ubiquitin specific protease 32 (USP32) is a highly conserved but uncharacterized gene, which has been reported to be associated with growth of breast cancer cells. However, the role of USP32 in human small cell lung cancer (SCLC) has not been uncovered. The aim of this study was to investigate and evaluate t...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12343
更新日期:2017-08-01 00:00:00
abstract:OBJECTIVES:Somatic stem cells can be obtained from a variety of adult human tissues. However, it was not clear whether human parathyroid glands, which secrete parathyroid hormones and are essential in maintaining homeostasis levels of calcium ions in the circulation, contained stem cells. We aimed to investigate the po...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.2009.00614.x
更新日期:2009-08-01 00:00:00
abstract:OBJECTIVES:To investigate the function and regulatory mechanism of Krüppel-like factor 3 (KLF3) in lung cancer. MATERIALS AND METHODS:KLF3 expression was analysed by qRT-PCR and Western blot assays. The proliferation, migration, invasion, cycle and apoptosis were measured by CCK-8 and EdU, wound-healing and Transwell,...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12551
更新日期:2019-03-01 00:00:00
abstract:OBJECTIVES:Skin-derived precursors are recognized to be a potentially autologous and accessible source of neural precursor cells for drug screening or cell-based treatments, in many neurological disorders. Thus, it is necessary to investigate appropriate methods for cryopreservation of such human skin-derived precursor...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.2011.00803.x
更新日期:2012-04-01 00:00:00
abstract:OBJECTIVES:Topographic cues can modulate morphology and differentiation of mesenchymal stem cells. This study aimed to determine how topographic cues of a novel bilayered poly (lactic-co-glycolic acid) (PLGA) scaffold affect osteogenic/odontogenic differentiation of dental pulp stem cells (DPSCs). METHODS:The surface ...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12676
更新日期:2019-11-01 00:00:00
abstract::The human promyelocytic leukaemia cell line, HL-60, was investigated with regard to proliferation and terminal differentiation following irradiation. The cells were X-irradiated and induced with 1.25% dimethyl sulfoxide (DMSO) towards the granulocytic lineage. Proliferation was measured via cell growth, clonogenicity ...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.1993.tb00031.x
更新日期:1993-11-01 00:00:00
abstract::A model of intestinal crypt organization is suggested based on the assumption that stem cells have a finite replicative life span. The model assumes the existence in a crypt of a quiescent ('deep') stem cell and a few more actively cycling ('proximate') stem cells. Monte Carlo computer simulation of published intestin...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.2006.00395.x
更新日期:2006-10-01 00:00:00
abstract:OBJECTIVES:This study focuses on experimental analysis and corresponding mathematical simulation of in vitro HUVECs (human umbilical vein endothelial cells) proliferation in the presence of various types of drugs. MATERIALS AND METHODS:HUVECs, once seeded in Petri dishes, were expanded to confluence. Temporal profiles...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.2010.00711.x
更新日期:2010-12-01 00:00:00
abstract::Differential display polymerase chain reaction (DD-PCR) is a powerful technique for comparing gene expression between cell types, or between stages of development or differentiation. Differentially expressed genes may be cloned and analysed further. Here we extend the use of DD-PCR to analyse differences in gene expre...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:
更新日期:1997-10-01 00:00:00
abstract:OBJECTIVES:Histone deacetylase (HDAC) is an important therapeutic target in cancer. Two of the main anticancer mechanisms of HDAC inhibitors are induction of terminal differentiation and inhibition of cell proliferation. To investigate the role of HDAC in maintenance of self-renewal and cell proliferation, we treated m...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.2009.00633.x
更新日期:2009-12-01 00:00:00