Abstract:
OBJECTIVES:Pre-eclampsia is a leading cause of morbidity and mortality during pregnancy. Although the two forms of this disorder, early- (EOPE) and late-onset of pre-eclampsia (LOPE) are different, the underlying pathology remains elusive. We aim to unravel the difference and to identify novel biomarkers for EOPE and LOPE. MATERIALS AND METHODS:A complete comparison of both placental and peripheral blood transcriptomes was performed to investigate the pathology of pre-eclampsia. Single-cell transcriptomics of the maternal-fetal interface were integrated to identify novel biomarkers for EOPE and LOPE which were further verified at protein or mRNA level in patients. RESULTS:We found that the transcriptomes of placentae from EOPE, but not LOPE, were significantly different from their respective controls. Conversely, the transcriptomes of peripheral blood from LOPE were more different from their controls than EOPE. Importantly, we identified that several classical biomarkers of pre-eclampsia were expressed specifically in extravillous trophoblast and syncytiotrophoblast and only upregulated in EOPE, suggesting they should not be applied to all pre-eclampsia patients in general. We further identified novel biomarkers for EOPE and LOPE from differentially expressed genes (DEGs) of placental and peripheral blood, respectively. The new biomarkers EBI3, IGF2, ORMDL3, GATA2 and KIR2DL4 were experimentally verified with patient blood samples. CONCLUSION:Our data demonstrate distinct pathology of EOPE and LOPE, and uncover new biomarkers that can be applied in diagnosis for pre-eclampsia.
journal_name
Cell Prolifjournal_title
Cell proliferationauthors
Guo F,Zhang B,Yang H,Fu Y,Wang Y,Huang J,Cheng M,Li X,Shen Z,Li L,He P,Xiang AP,Wang S,Zhang Hdoi
10.1111/cpr.12968subject
Has Abstractpub_date
2020-12-17 00:00:00pages
e12968eissn
0960-7722issn
1365-2184pub_type
杂志文章abstract::Nerous system diseases, both central and peripheral, bring an incredible burden onto patients and enormously reduce their quality of life. Currently, there are still no effective treatments to repair nerve lesions that do not have side effects. Stem cell-based therapies, especially those using dental stem cells, bring...
journal_title:Cell proliferation
pub_type: 杂志文章,评审
doi:10.1111/cpr.12572
更新日期:2019-05-01 00:00:00
abstract::An ELISA was developed and optimized to measure cell proliferation using a monoclonal antibody to bromodeoxyuridine (BrdUrd). Incorporation of BrdUrd into myoblast monolayers, measured as the optical density at 492 nm, increased in response to fetal calf serum, IGF-I and EGF, the ELISA data correlated closely with dat...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.1991.tb01179.x
更新日期:1991-09-01 00:00:00
abstract:OBJECTIVES:This study aims to explore the roles of N-myc and caspase-8 in TRAIL-resistant IMR-32 cells which exhibit MYCN oncogene amplification and lack caspase-8 expression. MATERIALS AND METHODS:We established N-myc-downregulated IMR-32 cells using shRNA lentiviral particles targeting N-myc and examined the effect ...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12577
更新日期:2019-05-01 00:00:00
abstract:OBJECTIVES:The main purpose of current study was to explore the effects of tetrahedral DNA nanostructures (TDNs) on neuroectodermal (NE-4C) stem cells migration and unveil the potential mechanisms. MATERIALS AND METHODS:The successfully self-assembled TDNs were also determined by dynamic light scattering (DLS). A bidi...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12503
更新日期:2018-12-01 00:00:00
abstract:OBJECTIVES:Dental follicle cells (DFCs) provide the origin of periodontal tissues, and Runx2 is essential for bone formation and tooth development. In this study, pluripotency of DFCs was evaluated and effects of Runx2 on them were investigated. MATERIALS AND METHODS:The DFCs were induced to differentiate towards oste...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.2010.00670.x
更新日期:2010-06-01 00:00:00
abstract:OBJECTIVES:Cancer stem cells (CSCs) compose a subpopulation of cells within a tumour that can self-renew and proliferate. Growth factors such as epidermal growth factor (EGF) and basic fibroblast growth factor (b-FGF) promote cancer stem cell proliferation in many solid tumours. This study assesses whether EGF, bFGF an...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.2012.00837.x
更新日期:2012-10-01 00:00:00
abstract:OBJECTIVES:High mobility group protein2 (HMGA2) and epithelial-to-mesenchymal transition are both related to progress of bladder cancer, however, the relationship between HMGA2, E-cadherin and vimentin in bladder cancer is not yet known. Thus, this study has examined expression of HMGA2, E-cadherin and vimentin in blad...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12096
更新日期:2014-04-01 00:00:00
abstract::Estimates of the clonogen content (number of microcolony-forming cells) of murine intestinal crypts using microcolony assays show an apparent dependence on the radiation dose used in the assay of clonogen content. Crypt radiation survival curves often show increased curvature beyond that expected on the basis of the c...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1046/j.1365-2184.2003.00279.x
更新日期:2003-08-01 00:00:00
abstract:OBJECTIVES:Hyperinsulinemia is a risk factor for pancreatic cancer, but the function of insulin in carcinogenesis is unclear, so this study aimed to elucidate the carcinogenic effects of insulin and the synergistic effect with the KRAS mutation in the early stage of pancreatic cancer. MATERIALS AND METHODS:A pair of i...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12575
更新日期:2019-05-01 00:00:00
abstract:OBJECTIVES:Bone marrow edema is a universal manifestation of rheumatoid arthritis (RA), and its pathological essence is a bone marrow lesion (BML) formed by various bone marrow (BM) immune cells. Neutrophils play an important role in inflammatory arthritis, but the role and mechanism of neutrophils in BML are not clear...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12824
更新日期:2020-07-01 00:00:00
abstract::Natural compounds derived from plant sources are well characterized as possessing a wide variety of remarkable anti-tumour properties, for example modulating programmed cell death, primarily referring to apoptosis, and autophagy. Distinct from apoptosis, autophagy (an evolutionarily conserved, multi-step lysosomal deg...
journal_title:Cell proliferation
pub_type: 杂志文章,评审
doi:10.1111/j.1365-2184.2012.00833.x
更新日期:2012-10-01 00:00:00
abstract:OBJECTIVES:This study investigated effects of reduced serum condition and vascular endothelial growth factor (VEGF) on angiogenic potential of adipose stromal cells (ASCs) in vitro. MATERIALS AND METHODS:Adipose stromal cells were cultured in three different types of medium: (i) F12/DMEM (FD) supplemented with 10% FBS...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12029
更新日期:2013-06-01 00:00:00
abstract:OBJECTIVES:BCL2 family proteins have been widely studied over the past decade due to their essential roles in apoptosis, oncogenesis and anti-cancer therapy. However, the similarities and differences in the spatial pattern of the BCL2 gene family within the context of chromatin have not been well characterized. We soug...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12826
更新日期:2020-06-01 00:00:00
abstract:OBJECTIVE:In this study, we generated an Rbm14 knockout mouse model to explore its functions during early mouse embryogenesis. MATERIALS AND METHODS:The Rbm14 knockout mouse model was generated by a combination of clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 and microinjection techniques. Th...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12724
更新日期:2020-01-01 00:00:00
abstract::The cell cycle is controlled by numerous mechanisms ensuring correct cell division. This review will focus on these mechanisms, i.e. regulation of cyclin-dependent kinases (CDK) by cyclins, CDK inhibitors and phosphorylating events. The quality checkpoints activated after DNA damage are also discussed. The complexity ...
journal_title:Cell proliferation
pub_type: 杂志文章,评审
doi:10.1046/j.1365-2184.2003.00266.x
更新日期:2003-06-01 00:00:00
abstract:OBJECTIVES:To understand if there exists a functional interaction between arsenic trioxide and paclitaxel in vitro. MATERIALS AND METHODS:HeLa and HCT116 (rho53(+/+) and rho53(-/-)) cells were treated with As2O3 and/or paclitaxel for various times. Treated cells were collected for analyses using a combination of flow ...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.2009.00606.x
更新日期:2009-06-01 00:00:00
abstract::Stem cell research is now a very broad field encompassing cells derived from all stages of life from the embryonic stem cells of the early blastocyst through to the adult stem cells of many tissues of the body. Adult stem cells from a variety of tissues are proving to be pluripotent and can differentiate into cell typ...
journal_title:Cell proliferation
pub_type: 杂志文章,评审
doi:10.1111/j.1365-2184.2008.00487.x
更新日期:2008-02-01 00:00:00
abstract::Autophagy follows a lysosomal degradation pathway in which a cell digests its own components. It is highly regulated by a limited number of autophagy-related genes (Atg) and the proteins they encode, that are crucial for cells to undergo the process via modulating autophagsome formation. Recently, accumulating evidenc...
journal_title:Cell proliferation
pub_type: 杂志文章,评审
doi:10.1111/cpr.12095
更新日期:2014-04-01 00:00:00
abstract:OBJECTIVES:Skin-derived precursors are recognized to be a potentially autologous and accessible source of neural precursor cells for drug screening or cell-based treatments, in many neurological disorders. Thus, it is necessary to investigate appropriate methods for cryopreservation of such human skin-derived precursor...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.2011.00803.x
更新日期:2012-04-01 00:00:00
abstract:OBJECTIVES:In order to provide a more comprehensive understanding of the effects of SARS-CoV-2 on oral health and possible saliva transmission, we performed RNA-seq profiles analysis from public databases and also a questionnaire survey on oral-related symptoms of COVID-19 patients. MATERIALS AND METHODS:To analyse AC...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12923
更新日期:2020-12-01 00:00:00
abstract:OBJECTIVES:Fibrotic cataract, including posterior capsule opacification (PCO) and anterior subcapsular cataract (ASC), renders millions of people visually impaired worldwide. However, the underlying mechanism remains poorly understood. Here, we report a miRNA-based regulatory pathway that controls pathological fibrosis...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12911
更新日期:2020-11-01 00:00:00
abstract:OBJECTIVES:Autophagy, a highly conserved lysosomal degradation process in eukaryotic cells, can digest long-lived proteins and damaged organelles through vesicular trafficking pathways. Nowadays, mechanisms of autophagy have been gradually elucidated and thus the discovery of small-molecule drugs targeting autophagy ha...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12403
更新日期:2018-06-01 00:00:00
abstract::We have studied hypoxia-induced cell cycle arrest in human cells where the retinoblastoma tumour suppressor protein (pRB) is either functional (T-47D cells) or abrogated by expression of the HPV18 E7 oncoprotein (NHIK 3025 cells). All cells in S phase are immediately arrested upon exposure to extreme hypoxia. During a...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1046/j.1365-2184.2003.00288.x
更新日期:2003-12-01 00:00:00
abstract::Sertoli cells (SeC) are responsible for the immunoprivileged status of the testis thanks to which allogeneic or xenogeneic engraftments can survive without pharmacological immune suppression if co-injected with SeC. This peculiar ability of SeC is dependent on secretion of a plethora of factors including maturation fa...
journal_title:Cell proliferation
pub_type: 杂志文章,评审
doi:10.1111/cpr.12599
更新日期:2019-05-01 00:00:00
abstract:OBJECTIVES:Ubiquitin specific protease 32 (USP32) is a highly conserved but uncharacterized gene, which has been reported to be associated with growth of breast cancer cells. However, the role of USP32 in human small cell lung cancer (SCLC) has not been uncovered. The aim of this study was to investigate and evaluate t...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12343
更新日期:2017-08-01 00:00:00
abstract:OBJECTIVES:Induced pluripotent stem cell (iPSC)-derived cardiomyocytes are a promising source of cells for regenerative heart disease therapies, but progress towards their use has been limited by their low differentiation efficiency and high cellular heterogeneity. Previous studies have demonstrated expression of adren...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12310
更新日期:2017-02-01 00:00:00
abstract:OBJECTIVES:We have evaluated the physiological roles of transforming growth factor-beta1 (TGF-beta1) on differentiation, migration, proliferation and anti-apoptosis characteristics of cultured spinal cord-derived neural progenitor cells. METHODS:We have used neural progenitor cells that had been isolated and cultured ...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.2008.00514.x
更新日期:2008-04-01 00:00:00
abstract:OBJECTIVES:Silicate bioactive glass (BG) has been widely demonstrated to stimulate both of the hard and soft tissue regeneration, in which ion products released from BG play important roles. However, the mechanism by which ion products act on cells on cells is unclear. MATERIALS AND METHODS:Human umbilical vein endoth...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12906
更新日期:2020-11-01 00:00:00
abstract:OBJECTIVES:Oestrogen receptor (ER) is a common nucleus receptor that is essential for the regulation of cell growth, proliferation and differentiation. This study was to examine whether ERα can affect the proliferation and odonto/osteogenic differentiation of stem cells from apical papilla (SCAPs). MATERIALS AND METHO...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12485
更新日期:2018-12-01 00:00:00
abstract::In situ presence of numerous DNA strand breaks is a typical feature of apoptotic cells. Selective DNA strand break induction by photolysis (SBIP) at sites that contain incorporated halogenated DNA precursors has recently been proposed as a method of analysing DNA replication. Detection of DNA strand breaks, thus, enab...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.1995.tb00045.x
更新日期:1995-11-01 00:00:00