Abstract:
OBJECTIVES:Induced pluripotent stem cell (iPSC)-derived cardiomyocytes are a promising source of cells for regenerative heart disease therapies, but progress towards their use has been limited by their low differentiation efficiency and high cellular heterogeneity. Previous studies have demonstrated expression of adrenergic receptors (ARs) in stem cells after differentiation; however, roles of ARs in fate specification of stem cells, particularly in cardiomyocyte differentiation and development, have not been characterized. MATERIALS AND METHODS:Murine-induced pluripotent stem cells (miPSCs) were cultured in hanging drops to form embryoid bodies, cells of which were then differentiated into cardiomyocytes. To determine whether ARs regulated miPSC differentiation into cardiac lineages, effects of the AR agonist, epinephrine (EPI), on miPSC differentiation and underlying signalling mechanisms, were evaluated. RESULTS:Treatment with EPI, robustly enhanced miPSC cardiac differentiation, as indicated by increased expression levels of cardiac-specific markers, GATA4, Nkx2.5 and Tnnt2. Although β-AR signalling is the foremost signalling pathway in cardiomyocytes, EPI-enhanced cardiac differentiation depended more on α-AR signalling than β-AR signalling. In addition, selective activation of α1 -AR signalling with specific agonists induced vigorous cardiomyocyte differentiation, whereas selective activation of α2 - or β-AR signalling induced no or less differentiation, respectively. EPI- and α1 -AR-dependent cardiomyocyte differentiation from miPSCs occurred through specific promotion of CPC proliferation via the MEK-ERK1/2 pathway and regulation of miPS cell-cycle progression. CONCLUSIONS:These results demonstrate that activation of ARs, particularly of α1 -ARs, promoted miPSC differentiation into cardiac lineages via MEK-ERK1/2 signalling.
journal_name
Cell Prolifjournal_title
Cell proliferationauthors
Li XL,Zeng D,Chen Y,Ding L,Li WJ,Wei T,Ou DB,Yan S,Wang B,Zheng QSdoi
10.1111/cpr.12310subject
Has Abstractpub_date
2017-02-01 00:00:00issue
1eissn
0960-7722issn
1365-2184journal_volume
50pub_type
杂志文章abstract:OBJECTIVES:This study aims to explore the roles of N-myc and caspase-8 in TRAIL-resistant IMR-32 cells which exhibit MYCN oncogene amplification and lack caspase-8 expression. MATERIALS AND METHODS:We established N-myc-downregulated IMR-32 cells using shRNA lentiviral particles targeting N-myc and examined the effect ...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12577
更新日期:2019-05-01 00:00:00
abstract::In situ presence of numerous DNA strand breaks is a typical feature of apoptotic cells. Selective DNA strand break induction by photolysis (SBIP) at sites that contain incorporated halogenated DNA precursors has recently been proposed as a method of analysing DNA replication. Detection of DNA strand breaks, thus, enab...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.1995.tb00045.x
更新日期:1995-11-01 00:00:00
abstract:OBJECTIVES:In this study, we have investigated whether secreted factors from embryonic stem cells (ESCs) could reprogramme keratinocytes and increase their potential to be directed into alternative cell lineages. MATERIALS AND METHODS:Contact and non-contact co-cultures of skin keratinocytes and murine ESCs were used ...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.2007.00464.x
更新日期:2007-10-01 00:00:00
abstract::In vitro cultured Ehrlich ascites tumour (EAT) cells were used because of the ease of their manipulation under different levels of hypoxia. They were used to clarify further the complex mechanism of oxygen-dependent cell proliferation. On reducing the oxygen concentration from 20% to lower levels (1-7%) an increase in...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.1992.tb01392.x
更新日期:1992-05-01 00:00:00
abstract::WR-1065 (2-[(aminopropyl)amino]ethanethiol) reduces cytotoxic and mutagenic effects caused by exposure of cells to radiation and chemotherapeutic drugs, but the mechanisms involved are not fully known. We have observed an accumulation of cells in G2 in WR-1065 treated Chinese hamster ovary cells grown in alpha-minimal...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.1992.tb01466.x
更新日期:1992-11-01 00:00:00
abstract:OBJECTIVES:Dental follicle cells (DFCs) provide the origin of periodontal tissues, and Runx2 is essential for bone formation and tooth development. In this study, pluripotency of DFCs was evaluated and effects of Runx2 on them were investigated. MATERIALS AND METHODS:The DFCs were induced to differentiate towards oste...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.2010.00670.x
更新日期:2010-06-01 00:00:00
abstract:OBJECTIVES:To investigate behaviour and osteogenic cytokine expression of RAW264.7 macrophages grown on TiO2 nanotube layers. MATERIALS AND METHODS:The murine macrophage cell line RAW 264.7 was cultured on TiO2 nanotubes of varying diameter; macrophage morphology was examined using scanning electron microscopy. Cell a...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12072
更新日期:2013-12-01 00:00:00
abstract::Cell proliferation was investigated in human tumour xenografts using bromodeoxyuridine (BrdUrd) labelling, evaluated either by flow cytometry or in tissue sections, and also using the proliferation marker Ki-67. BrdUrd labelling was found to increase when cryostat tumour sections were digested with an enzymic solution...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.1991.tb01147.x
更新日期:1991-03-01 00:00:00
abstract::Two groups of male rats were treated orally for 60 days with ebrotidine and cimetidine, used as reference standard, respectively. The dose used of both drugs was 500 mg/kg. A third group, used as control, received 10 ml/kg of an aqueous agar suspension. After receiving the last dose, the animals were killed by inhalat...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.1995.tb00080.x
更新日期:1995-07-01 00:00:00
abstract:OBJECTIVES:MicroRNAs (miRNAs) as small non-coding RNA molecules act by negatively regulating their target genes. Recent studies have shown that protein phosphatase Mg2+/Mn2+-dependent 1F (PPM1F) plays a critical role in cancer metastasis. But, the regulation mechanisms of PPM1F by miRNAs in gastric cancer (GC) remain u...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12444
更新日期:2018-08-01 00:00:00
abstract:OBJECTIVE:Embryo implantation needs a reciprocal interaction between competent embryo and receptive endometrium. Adenosine triphosphate (ATP) produced by stressed or injured cells acts as an important signalling molecule. This study aims to investigate whether adenosine triphosphate (ATP) plays an important role in the...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12737
更新日期:2020-02-01 00:00:00
abstract::Reports on the p53-related cell cycle and apoptotic responses of EBV-transformed lymphoblastoid cell lines to DNA damage have led to some confusion. This may be due to differences in the nature of the specific p53 mutations under examination, but it can also be partly attributed to methodological and analytical proble...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:
更新日期:1999-02-01 00:00:00
abstract::This study examines epithelial cell streaming and turnover in normal rat bulbar conjunctiva. Twenty seven male adult random-bred Hebrew rats weighing between 250-300 g, were injected i.p. with [3H]-thymidine. Three rats were killed at various times, thereafter from 1 h to 28 days. The enucleated eyes were fixed in for...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.1995.tb00066.x
更新日期:1995-04-01 00:00:00
abstract::Neural stem cells (NSCs) are early precursors of neuronal and glial cells. NSCs are capable of generating identical progeny through virtually unlimited numbers of cell divisions (cell proliferation), producing daughter cells committed to differentiation. Nuclear factor kappa B (NF-kappaB) is an inducible, ubiquitous t...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.2006.00403.x
更新日期:2006-12-01 00:00:00
abstract:OBJECTIVES:Advanced head and neck carcinomas (HNCs) are aggressive tumours, mainly due to hypoxia and a cancer stem cell (CSC) subpopulation. The aim of this study was to simulate tumour growth and behaviour during radiotherapy of three HNC groups (governed by different growth kinetics, hypoxia levels and CSC division ...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12251
更新日期:2016-06-01 00:00:00
abstract:OBJECTIVE:Spinal cord tumours are highly malignant and often lead to paralysis and death due to their infiltrative nature, high recurrence rate and limited treatment options. In this study, we measured antitumour efficacy of the Salmonella typhimurium A1-R tumour-targeting bacterium strain, administered systemically or...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.2009.00652.x
更新日期:2010-02-01 00:00:00
abstract::Nerous system diseases, both central and peripheral, bring an incredible burden onto patients and enormously reduce their quality of life. Currently, there are still no effective treatments to repair nerve lesions that do not have side effects. Stem cell-based therapies, especially those using dental stem cells, bring...
journal_title:Cell proliferation
pub_type: 杂志文章,评审
doi:10.1111/cpr.12572
更新日期:2019-05-01 00:00:00
abstract:OBJECTIVES:Translational research using adult stem cells derived from various tissues has been highlighted in cell-based therapy. However, there are many limitations to using conventional culture systems of adult stem cells for clinically applicability, including limited combinations of cytokines and use of nutrients d...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.2010.00693.x
更新日期:2010-10-01 00:00:00
abstract:OBJECTIVES:This study is to explore the role of Notch signalling during the regeneration of rat tracheal epithelium after injury induced by 5-fluorouracil (5-FU). MATERIALS AND METHODS:We developed an ex vivo model of rat tracheal epithelial regeneration using 5-FU to induce injury. Expression levels of members of the...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.2008.00569.x
更新日期:2009-02-01 00:00:00
abstract::L-type voltage-gated calcium ion channels (L-VGCCs) have been demonstrated to be the mediator of several significant intracellular activities in excitable cells, such as neurons, chromaffin cells and myocytes. Recently, an increasing number of studies have investigated the function of L-VGCCs in non-excitable cells, p...
journal_title:Cell proliferation
pub_type: 杂志文章,评审
doi:10.1111/cpr.12623
更新日期:2019-07-01 00:00:00
abstract:OBJECTIVES:Often, non-small cell lung cancers (NSCLC) respond only poorly to the tyrosine kinase inhibitor (TKI) gefitinib, which targets the epidermal growth factor receptor (EGFR), these poor responders EGFRs lacking activating mutations. In this study, we have attempted to improve TKI response of NSCLC cell lines (A...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12040
更新日期:2013-08-01 00:00:00
abstract:OBJECTIVES:Restoring a functional beta-cell mass is a fundamental goal in treating diabetes. A complex signalling pathway network coordinates the regulation of beta-cell proliferation, although a role for ERK5 in this network has not been reported. This question was addressed in this study. MATERIALS AND METHODS:We st...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12410
更新日期:2018-06-01 00:00:00
abstract::Rabbit embryo-fetal fluid (EFF) contains regulatory factors of cell proliferation which increase the duration of the cell cycle, induce a quiescent status in some cells and lead up to cell death in others. The objective of this study was to demonstrate which of the two processes, namely necrosis or apoptosis, was resp...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.1993.tb00013.x
更新日期:1993-03-01 00:00:00
abstract::Differential display polymerase chain reaction (DD-PCR) is a powerful technique for comparing gene expression between cell types, or between stages of development or differentiation. Differentially expressed genes may be cloned and analysed further. Here we extend the use of DD-PCR to analyse differences in gene expre...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:
更新日期:1997-10-01 00:00:00
abstract:OBJECTIVES:Yes-associated protein (YAP) has been reported to regulate cell proliferation and differentiation. We aimed to characterize the role of YAP in angiotensin II (Ang II)-induced hypertensive vascular remodelling (HVR) and vascular smooth muscle cells (VSMCs) phenotypic modulation and to explore the underlying m...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12517
更新日期:2018-12-01 00:00:00
abstract:OBJECTIVES:Histone deacetylase (HDAC) is an important therapeutic target in cancer. Two of the main anticancer mechanisms of HDAC inhibitors are induction of terminal differentiation and inhibition of cell proliferation. To investigate the role of HDAC in maintenance of self-renewal and cell proliferation, we treated m...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.2009.00633.x
更新日期:2009-12-01 00:00:00
abstract:OBJECTIVES:Exposure to microgravity induces many adaptive and pathological changes in human bone marrow mesenchymal stem cells (hBMSCs). However, the underlying mechanisms of these changes are poorly understood. We revealed the gene expression patterns of hBMSCs under normal ground (NG) and simulated microgravity (SMG)...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12539
更新日期:2019-03-01 00:00:00
abstract:OBJECTIVES:Osteosarcoma is the most commonly diagnosed primary malignancy of bone and its overall survival rate is still very low. The molecular mechanisms underlying the progression of osteosarcoma have not been clearly illuminated. Heat shock transcription factor 1 (HSF1) is a key regulator of the heat shock response...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12346
更新日期:2017-08-01 00:00:00
abstract::Autophagy follows a lysosomal degradation pathway in which a cell digests its own components. It is highly regulated by a limited number of autophagy-related genes (Atg) and the proteins they encode, that are crucial for cells to undergo the process via modulating autophagsome formation. Recently, accumulating evidenc...
journal_title:Cell proliferation
pub_type: 杂志文章,评审
doi:10.1111/cpr.12095
更新日期:2014-04-01 00:00:00
abstract:OBJECTIVES:The use of platelets and platelet products has become increasingly popular clinically as a means of accelerating endosseous wound healing. It is likely that growth factors released by activated platelets at the site of injury play a role in periodontal regeneration by regulating cellular activity. The purpos...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.2007.00430.x
更新日期:2007-04-01 00:00:00