Biochemical and morphological characterizations of DU-145 cell mortality in rabbit embryo-fetal fluid.

Abstract:

:Rabbit embryo-fetal fluid (EFF) contains regulatory factors of cell proliferation which increase the duration of the cell cycle, induce a quiescent status in some cells and lead up to cell death in others. The objective of this study was to demonstrate which of the two processes, namely necrosis or apoptosis, was responsible for the cell death. Inhibitors of protein synthesis, and nuclease and phospholipase A2 activities did not restore the viability of the cells treated with EFF. Using a combination of DNA labelling and extraction, it was possible to show that a large proportion of DNA was fragmented in the cells released in the supernatant while only a very small portion of DNA was fragmented in the monolayer cells. EFF did not induce fragmentation of DNA into nucleosome-sized subunits as analysed using polyacrylamide gel electrophoresis. Nevertheless, using cytofluorometric analysis, it was possible to demonstrate that 50% of the cells released in the supernatant contained a lower quantity of DNA per cell than in the control cells. This was also observed with EFF-treated monolayer cells but not in the control monolayer cells. The reduction of the DNA content per monolayer cell became significant at 48 h of treatment with EFF. Electron microscopic analysis did not reveal blebbing of the cells. However, depletion of glycogen, condensation of mitochondria and increasing number of lysosomes and residual bodies were observed upon treatment with EFF. From these experiments we conclude that the DU-145 cells treated with EFF do not die by apoptosis, but rather seem to die by necrosis.

journal_name

Cell Prolif

journal_title

Cell proliferation

authors

Lapointe J,Bergeron D,Dufour M,Dubé D,Govindan MV,Lambert RD

doi

10.1111/j.1365-2184.1993.tb00013.x

subject

Has Abstract

pub_date

1993-03-01 00:00:00

pages

125-38

issue

2

eissn

0960-7722

issn

1365-2184

journal_volume

26

pub_type

杂志文章
  • Application of a DNA double labelling method for the flow cytometric analysis of recruitment of non-cycling cells in a mixed population of P and Q cells.

    abstract::In this paper we describe the application of a non-radioactive DNA double labelling and staining method to an analysis of cell proliferation kinetics by flow cytometry, aimed at the direct measurement of recruitment rates in cell cultures. The method is based on the application of two halogenated deoxyuridines: iodode...

    journal_title:Cell proliferation

    pub_type: 杂志文章

    doi:10.1111/j.1365-2184.1993.tb00009.x

    authors: Bakker PJ,de Vries RJ,Tukker CJ,Hoebe RA,Barendsen GW

    更新日期:1993-01-01 00:00:00

  • The influence of antisense oligonucleotides against STAT5 on the regulation of normal haematopoiesis in a bone marrow model.

    abstract::Cytokines and growth factors that take part in the regulation of haematopoietic cell development activate many signalling pathways in target cells. The STAT5 (signal transducers and activators of transcription) proteins are members of a family of signal transducers and activators of transcription that can be activated...

    journal_title:Cell proliferation

    pub_type: 杂志文章

    doi:10.1111/j.1365-2184.2004.00308.x

    authors: Baśkiewicz-Masiuk M,Paczkowski M,Machaliński B

    更新日期:2004-06-01 00:00:00

  • SHP2 deficiency promotes Staphylococcus aureus pneumonia following influenza infection.

    abstract:OBJECTIVES:Secondary bacterial pneumonia is common following influenza infection. However, it remains unclear about the underlying molecular mechanisms. MATERIALS AND METHODS:We established a mouse model of post-influenza S aureus pneumonia using conditional Shp2 knockout mice (LysMCre/+ :Shp2flox/flox ). The survival...

    journal_title:Cell proliferation

    pub_type: 杂志文章

    doi:10.1111/cpr.12721

    authors: Ouyang W,Liu C,Pan Y,Han Y,Yang L,Xia J,Xu F

    更新日期:2020-01-01 00:00:00

  • Involvement of SIRT3-GSK3β deacetylation pathway in the effects of maternal diabetes on oocyte meiosis.

    abstract:OBJECTIVES:It has been widely reported that maternal diabetes impairs oocyte quality. However, the responsible mechanisms remain to be explored. In the present study, we focused on whether SIRT3-GSK3β pathway mediates the meiotic defects in oocytes from diabetic mice. MATERIALS AND METHODS:GSK3β functions in mouse ooc...

    journal_title:Cell proliferation

    pub_type: 杂志文章

    doi:10.1111/cpr.12940

    authors: Xin Y,Jin Y,Ge J,Huang Z,Han L,Li C,Wang D,Zhu S,Wang Q

    更新日期:2021-01-01 00:00:00

  • The need for dynamic methods for measuring cell cycle perturbations: a study in radiation-treated lymphoblastoid cell lines of varying p53 status.

    abstract::Reports on the p53-related cell cycle and apoptotic responses of EBV-transformed lymphoblastoid cell lines to DNA damage have led to some confusion. This may be due to differences in the nature of the specific p53 mutations under examination, but it can also be partly attributed to methodological and analytical proble...

    journal_title:Cell proliferation

    pub_type: 杂志文章

    doi:

    authors: Gilchrist R,Lomax ME,Camplejohn RS

    更新日期:1999-02-01 00:00:00

  • Bioglass could increase cell membrane fluidity with ion products to develop its bioactivity.

    abstract:OBJECTIVES:Silicate bioactive glass (BG) has been widely demonstrated to stimulate both of the hard and soft tissue regeneration, in which ion products released from BG play important roles. However, the mechanism by which ion products act on cells on cells is unclear. MATERIALS AND METHODS:Human umbilical vein endoth...

    journal_title:Cell proliferation

    pub_type: 杂志文章

    doi:10.1111/cpr.12906

    authors: Yan L,Li H,Xia W

    更新日期:2020-11-01 00:00:00

  • Streaming of labelled cells in the conjunctival epithelium.

    abstract::This study examines epithelial cell streaming and turnover in normal rat bulbar conjunctiva. Twenty seven male adult random-bred Hebrew rats weighing between 250-300 g, were injected i.p. with [3H]-thymidine. Three rats were killed at various times, thereafter from 1 h to 28 days. The enucleated eyes were fixed in for...

    journal_title:Cell proliferation

    pub_type: 杂志文章

    doi:10.1111/j.1365-2184.1995.tb00066.x

    authors: Zajicek G,Perry A,Pe'er J

    更新日期:1995-04-01 00:00:00

  • Downregulation of USP32 inhibits cell proliferation, migration and invasion in human small cell lung cancer.

    abstract:OBJECTIVES:Ubiquitin specific protease 32 (USP32) is a highly conserved but uncharacterized gene, which has been reported to be associated with growth of breast cancer cells. However, the role of USP32 in human small cell lung cancer (SCLC) has not been uncovered. The aim of this study was to investigate and evaluate t...

    journal_title:Cell proliferation

    pub_type: 杂志文章

    doi:10.1111/cpr.12343

    authors: Hu W,Wei H,Li K,Li P,Lin J,Feng R

    更新日期:2017-08-01 00:00:00

  • Cell kinetic studies in mouse tongue mucosa by autoradiographic, immunohistochemical and flow cytometric techniques.

    abstract::A number of techniques, including autoradiography after in vivo administration of tritiated thymidine ([3H]dT), immunohistochemistry after in vivo administration of bromodeoxyuridine (BrdUrd), and flow cytometry (FCM) with and without BrdUrd detection were compared in the epithelium of ventral mouse tongue. Investigat...

    journal_title:Cell proliferation

    pub_type: 杂志文章

    doi:10.1111/j.1365-2184.1994.tb01429.x

    authors: Dörr W,Arui J,Beisker W,Schultz-Hector S

    更新日期:1994-06-01 00:00:00

  • Chondrocyte proliferation in a new culture system.

    abstract:OBJECTIVE:This study has aimed to study different culture systems that might stimulate an increase in cell proliferation of normal and osteoarthritis chondrocytes from articular cartilage in rat model. MATERIAL AND METHODS:Three culture systems using chondrocytes embedded in alginate beads were tested: chondrocytes cu...

    journal_title:Cell proliferation

    pub_type: 杂志文章

    doi:10.1111/j.1365-2184.2008.00580.x

    authors: Gomez-Camarillo MA,Almonte-Becerril M,Vasquez Tort M,Tapia-Ramirez J,Kouri Flores JB

    更新日期:2009-04-01 00:00:00

  • Identification of genes deregulated during serum-free medium adaptation of a Burkitt's lymphoma cell line.

    abstract:OBJECTIVE:Serum is usually added to growth media when mammalian cells are cultured in vitro to supply the cells with growth factors, hormones, nutrients and trace elements. Defined proteins and metal ions, such as insulin, growth factors, transferrin and sodium selenite, are sometimes also included and can in some case...

    journal_title:Cell proliferation

    pub_type: 杂志文章

    doi:10.1111/j.1365-2184.2007.00500.x

    authors: Zander L,Bemark M

    更新日期:2008-02-01 00:00:00

  • Cell cycle analysis of asexual stages of erythrocytic malaria parasites.

    abstract::Intra-erythrocytic Plasmodium species can be stained with the DNA binding dye, Hoechst 33342, and the distribution of DNA content determined for parasite populations by flow cytometric measurement of fluorescence. Analysis of this distribution will determine the parasitaemia (percentage of erythrocytes infected), and ...

    journal_title:Cell proliferation

    pub_type: 杂志文章

    doi:10.1111/j.1365-2184.1992.tb01452.x

    authors: Jacobberger JW,Horan PK,Hare JD

    更新日期:1992-09-01 00:00:00

  • Role of alpha- and beta-adrenergic receptors in cardiomyocyte differentiation from murine-induced pluripotent stem cells.

    abstract:OBJECTIVES:Induced pluripotent stem cell (iPSC)-derived cardiomyocytes are a promising source of cells for regenerative heart disease therapies, but progress towards their use has been limited by their low differentiation efficiency and high cellular heterogeneity. Previous studies have demonstrated expression of adren...

    journal_title:Cell proliferation

    pub_type: 杂志文章

    doi:10.1111/cpr.12310

    authors: Li XL,Zeng D,Chen Y,Ding L,Li WJ,Wei T,Ou DB,Yan S,Wang B,Zheng QS

    更新日期:2017-02-01 00:00:00

  • Epstein-Barr virus-induced transformation of B cells for the diagnosis of genetic metabolic disorders--enumerative conditions for cryopreservation.

    abstract::Epstein-Barr virus (EBV) infection in vitro causes transformation of B cells and generates B lymphoblastoid cell lines (LCLs). These LCLs have been widely used for the diagnostic of several genetic metabolic disorders. However, up to now, efficiency of LCL generation has been based on misleading subjective analysis. I...

    journal_title:Cell proliferation

    pub_type: 杂志文章

    doi:10.1111/j.1365-2184.2006.00363.x

    authors: Mello AS,Burin MG,Michellin K,Viapiana M,Giugliani R,Coelho JC,Bauer ME

    更新日期:2006-02-01 00:00:00

  • Overproduced bone marrow neutrophils in collagen-induced arthritis are primed for NETosis: An ignored pathological cell involving inflammatory arthritis.

    abstract:OBJECTIVES:Bone marrow edema is a universal manifestation of rheumatoid arthritis (RA), and its pathological essence is a bone marrow lesion (BML) formed by various bone marrow (BM) immune cells. Neutrophils play an important role in inflammatory arthritis, but the role and mechanism of neutrophils in BML are not clear...

    journal_title:Cell proliferation

    pub_type: 杂志文章

    doi:10.1111/cpr.12824

    authors: Xu D,Lin Y,Shen J,Zhang J,Wang J,Zhang Y,Zhang H,Ning L,Liu P,Li S,Zeng H,Lin J,Yu C

    更新日期:2020-07-01 00:00:00

  • Possibility of selection of chondrogenic progenitor cells by telomere length in FGF-2-expanded mesenchymal stromal cells.

    abstract::Telomere length plays an important role in regulating the proliferative capacity of cells, and serves as a marker for cell cycle history and also for their remaining replicative potential. Mesenchymal stromal cells (MSC) are known to be a significant cell source for therapeutic intervention and tissue engineering. To ...

    journal_title:Cell proliferation

    pub_type: 杂志文章

    doi:10.1111/j.1365-2184.2006.00397.x

    authors: Yanada S,Ochi M,Kojima K,Sharman P,Yasunaga Y,Hiyama E

    更新日期:2006-12-01 00:00:00

  • Cytotoxicity of clove (Syzygium aromaticum) oil and its major components to human skin cells.

    abstract::The essential oil extracted from clove (Syzygium aromaticum) is used as a topical application to relieve pain and promote healing in herbal medicine and also finds use in the fragrance and flavouring industries. Clove oil has two major components, eugenol and beta-caryophyllene, which constitute 78% and 13% of the oil...

    journal_title:Cell proliferation

    pub_type: 杂志文章

    doi:10.1111/j.1365-2184.2006.00384.x

    authors: Prashar A,Locke IC,Evans CS

    更新日期:2006-08-01 00:00:00

  • Cell proliferation in human tumour xenografts: measurement using antibody labelling against bromodeoxyuridine and Ki-67.

    abstract::Cell proliferation was investigated in human tumour xenografts using bromodeoxyuridine (BrdUrd) labelling, evaluated either by flow cytometry or in tissue sections, and also using the proliferation marker Ki-67. BrdUrd labelling was found to increase when cryostat tumour sections were digested with an enzymic solution...

    journal_title:Cell proliferation

    pub_type: 杂志文章

    doi:10.1111/j.1365-2184.1991.tb01147.x

    authors: Parkins CS,Bush C,Price P,Steel GG

    更新日期:1991-03-01 00:00:00

  • A mathematical model of glioma growth: the effect of extent of surgical resection.

    abstract::We have developed a mathematical model based on proliferation and infiltration of neoplastic cells that allows predictions to be made concerning the life expectancies following various extents of surgical resection of gliomas of all grades of malignancy. The key model parameters are the growth rate and the diffusion r...

    journal_title:Cell proliferation

    pub_type: 杂志文章

    doi:10.1111/j.1365-2184.1996.tb01580.x

    authors: Woodward DE,Cook J,Tracqui P,Cruywagen GC,Murray JD,Alvord EC Jr

    更新日期:1996-06-01 00:00:00

  • Cytometry in the brain: studying differentiation to diagnostic applications in brain disease and regeneration therapy.

    abstract::During brain development, a population of uniform embryonic cells migrates and differentiates into a large number of neural phenotypes - origin of the enormous complexity of the adult nervous system. Processes of cell proliferation, differentiation and programmed death of no longer required cells, do not occur only du...

    journal_title:Cell proliferation

    pub_type: 杂志文章

    doi:10.1111/cpr.12087

    authors: Ulrich H,Bocsi J,Glaser T,Tárnok A

    更新日期:2014-02-01 00:00:00

  • Linc02349 promotes osteogenesis of human umbilical cord-derived stem cells by acting as a competing endogenous RNA for miR-25-3p and miR-33b-5p.

    abstract:OBJECTIVES:Increasing evidences suggest that inducing mesenchymal stem cells to differentiate into osteoblasts has been as an especially important component in the prevention and therapy for degenerative bone disease. Here, we identify a novel lncRNA, linc02349, which increases significantly during osteogenic different...

    journal_title:Cell proliferation

    pub_type: 杂志文章

    doi:10.1111/cpr.12814

    authors: Cao L,Liu W,Zhong Y,Zhang Y,Gao D,He T,Liu Y,Zou Z,Mo Y,Peng S,Shuai C

    更新日期:2020-05-01 00:00:00

  • Downregulation of cell survival signalling pathways and increased cell damage in hydrogen peroxide-treated human renal proximal tubular cells by alpha-erythropoietin.

    abstract:OBJECTIVE:Erythropoietin has been shown to have a protective effect in certain models of ischaemia-reperfusion, and in some cases the protection has been correlated with activation of signalling pathways known to play a role in cell survival and proliferation. We have studied whether erythropoietin would overcome direc...

    journal_title:Cell proliferation

    pub_type: 杂志文章

    doi:10.1111/j.1365-2184.2009.00617.x

    authors: Andreucci M,Fuiano G,Presta P,Lucisano G,Leone F,Fuiano L,Bisesti V,Esposito P,Russo D,Memoli B,Faga T,Michael A

    更新日期:2009-08-01 00:00:00

  • Labelling DNA strand breaks with BrdUTP. Detection of apoptosis and cell proliferation.

    abstract::In situ presence of numerous DNA strand breaks is a typical feature of apoptotic cells. Selective DNA strand break induction by photolysis (SBIP) at sites that contain incorporated halogenated DNA precursors has recently been proposed as a method of analysing DNA replication. Detection of DNA strand breaks, thus, enab...

    journal_title:Cell proliferation

    pub_type: 杂志文章

    doi:10.1111/j.1365-2184.1995.tb00045.x

    authors: Li X,Darzynkiewicz Z

    更新日期:1995-11-01 00:00:00

  • Cell growth and cholesterol metabolism in human glucose-6-phosphate dehydrogenase deficient lymphomononuclear cells.

    abstract::Atherosclerosis is an inflammatory-fibroproliferative response of the arterial wall involving a complex set of interconnected events where cell proliferation (lymphomonocytes, and endothelial and smooth-muscle cells) and substantial perturbations of intracellular cholesterol metabolism are considered to be among the m...

    journal_title:Cell proliferation

    pub_type: 杂志文章

    doi:10.1046/j.1365-2184.2002.00231.x

    authors: Batetta B,Bonatesta RR,Sanna F,Putzolu M,Mulas MF,Collu M,Dessì S

    更新日期:2002-06-01 00:00:00

  • Increased cellular hypoxia and reduced proliferation of both normal and leukaemic cells during progression of acute myeloid leukaemia in rats.

    abstract::The microenvironmental changes in the bone marrow, spleen and liver during progression of the transplantable promyelocytic leukaemia in the Brown Norwegian rat (BNML) have been studied. We used flow cytometry to estimate cellular hypoxia and proliferation based on in vivo pulse-labelling with a mixture of 2-nitroimida...

    journal_title:Cell proliferation

    pub_type: 杂志文章

    doi:10.1046/j.1365-2184.2000.00183.x

    authors: Jensen PO,Mortensen BT,Hodgkiss RJ,Iversen PO,Christensen IJ,Helledie N,Larsen JK

    更新日期:2000-12-01 00:00:00

  • Plant natural compounds: targeting pathways of autophagy as anti-cancer therapeutic agents.

    abstract::Natural compounds derived from plant sources are well characterized as possessing a wide variety of remarkable anti-tumour properties, for example modulating programmed cell death, primarily referring to apoptosis, and autophagy. Distinct from apoptosis, autophagy (an evolutionarily conserved, multi-step lysosomal deg...

    journal_title:Cell proliferation

    pub_type: 杂志文章,评审

    doi:10.1111/j.1365-2184.2012.00833.x

    authors: Zhang X,Chen LX,Ouyang L,Cheng Y,Liu B

    更新日期:2012-10-01 00:00:00

  • MiR-22-3p inhibits fibrotic cataract through inactivation of HDAC6 and increase of α-tubulin acetylation.

    abstract:OBJECTIVES:Fibrotic cataract, including posterior capsule opacification (PCO) and anterior subcapsular cataract (ASC), renders millions of people visually impaired worldwide. However, the underlying mechanism remains poorly understood. Here, we report a miRNA-based regulatory pathway that controls pathological fibrosis...

    journal_title:Cell proliferation

    pub_type: 杂志文章

    doi:10.1111/cpr.12911

    authors: Wang X,Wang L,Sun Y,Chen B,Xiong L,Chen J,Huang M,Wu J,Tan X,Zheng Y,Huang S,Liu Y

    更新日期:2020-11-01 00:00:00

  • Topographic cues of a novel bilayered scaffold modulate dental pulp stem cells differentiation by regulating YAP signalling through cytoskeleton adjustments.

    abstract:OBJECTIVES:Topographic cues can modulate morphology and differentiation of mesenchymal stem cells. This study aimed to determine how topographic cues of a novel bilayered poly (lactic-co-glycolic acid) (PLGA) scaffold affect osteogenic/odontogenic differentiation of dental pulp stem cells (DPSCs). METHODS:The surface ...

    journal_title:Cell proliferation

    pub_type: 杂志文章

    doi:10.1111/cpr.12676

    authors: Du Y,Montoya C,Orrego S,Wei X,Ling J,Lelkes PI,Yang M

    更新日期:2019-11-01 00:00:00

  • Proliferation and differentiation potential of cryopreserved human skin-derived precursors.

    abstract:OBJECTIVES:Skin-derived precursors are recognized to be a potentially autologous and accessible source of neural precursor cells for drug screening or cell-based treatments, in many neurological disorders. Thus, it is necessary to investigate appropriate methods for cryopreservation of such human skin-derived precursor...

    journal_title:Cell proliferation

    pub_type: 杂志文章

    doi:10.1111/j.1365-2184.2011.00803.x

    authors: Bakhtiari M,Mansouri K,Sadeghi Y,Mostafaie A

    更新日期:2012-04-01 00:00:00

  • Nicotine-induced proliferation of isolated rat pancreatic acinar cells: effect on cell signalling and function.

    abstract:OBJECTIVES:The aim of the current study was to investigate whether nicotine treatment would induce the proliferation of isolated rat primary pancreatic acinar cells in culture by activating mitogen-activated protein kinase (MAPK) signalling and exocrine secretion. MATERIALS AND METHODS:A nicotine dose- and time-respon...

    journal_title:Cell proliferation

    pub_type: 杂志文章

    doi:10.1111/j.1365-2184.2007.00418.x

    authors: Chowdhury P,Bose C,Udupa KB

    更新日期:2007-02-01 00:00:00