Abstract:
OBJECTIVES:Skin-derived precursors are recognized to be a potentially autologous and accessible source of neural precursor cells for drug screening or cell-based treatments, in many neurological disorders. Thus, it is necessary to investigate appropriate methods for cryopreservation of such human skin-derived precursors (hSKPs). The aim of this study was to evaluate different cryopreservation techniques for retention of hSKPs to discover an optimized protocol. MATERIALS AND METHODS:We cryopreserved hSKPs treated with 0%, 10%, 20%, 30% and 40% foetal bovine serum (FBS) and three concentrations of dimethylsulphoxide (DMSO) 5%, 10% and 15%, with two different storage periods in liquid nitrogen (2 days: short-term storage; and 2 months: long-term storage). Then, we assessed survival and proliferation levels of the cells after freeze-thaw processes, by viability measurement and colony-forming assay. For detecting hSKPs, we used immunocytochemistry and RT-PCR assessments. RESULTS:Our findings indicated that hSKPs cryopreserved in 5% DMSO without FBS, had better survival and proliferation potentials compared to other working formulations. With various concentrations of cryoprotectants over different time periods, hSKPs retained their differentiation potentiality and were able to differentiate into neurons (NFM and βΙΙΙ tubulin-positive), glial cells (GFAP-positive) and smooth muscle cells (SMA-positive). CONCLUSIONS: Results revealed that in only 5% DMSO, hSKPs could be cryopreserved for long-term storage with considerable survival and proliferation levels, without losing multipotency.
journal_name
Cell Prolifjournal_title
Cell proliferationauthors
Bakhtiari M,Mansouri K,Sadeghi Y,Mostafaie Adoi
10.1111/j.1365-2184.2011.00803.xsubject
Has Abstractpub_date
2012-04-01 00:00:00pages
148-57issue
2eissn
0960-7722issn
1365-2184journal_volume
45pub_type
杂志文章abstract:OBJECTIVES:Based on in vivo studies, low-intensity pulsed ultrasound (LIPUS) stimulation has been widely used in the clinic for advancing bone growth during healing of non-union alignment, fractures and other osseous defects. In this study, we have investigated osteogenic differentiation of adipose stem cells (ASCs) re...
journal_title:Cell proliferation
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journal_title:Cell proliferation
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更新日期:2018-12-01 00:00:00
abstract::In this paper we describe the application of a non-radioactive DNA double labelling and staining method to an analysis of cell proliferation kinetics by flow cytometry, aimed at the direct measurement of recruitment rates in cell cultures. The method is based on the application of two halogenated deoxyuridines: iodode...
journal_title:Cell proliferation
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doi:10.1111/j.1365-2184.1993.tb00009.x
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journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.2010.00676.x
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journal_title:Cell proliferation
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journal_title:Cell proliferation
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journal_title:Cell proliferation
pub_type: 杂志文章
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更新日期:2021-01-11 00:00:00
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journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12737
更新日期:2020-02-01 00:00:00
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journal_title:Cell proliferation
pub_type: 杂志文章,评审
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更新日期:2020-11-01 00:00:00
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journal_title:Cell proliferation
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doi:10.1111/cpr.12460
更新日期:2018-08-01 00:00:00
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journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.1993.tb00013.x
更新日期:1993-03-01 00:00:00
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journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12726
更新日期:2020-01-01 00:00:00
abstract:OBJECTIVES:Recent data show that Imatinib mesylate (IM) also affects haematopoietic stem cells (HSC), T lymphocytes and dendritic cells that do not harbour constitutively active tyrosine kinases. MATERIALS AND METHODS:We evaluated possible effects of IM on human bone marrow-derived mesenchymal stem cells (MSC) in vitr...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.2007.00438.x
更新日期:2007-06-01 00:00:00
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journal_title:Cell proliferation
pub_type: 杂志文章,评审
doi:10.1111/j.1365-2184.2008.00487.x
更新日期:2008-02-01 00:00:00
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journal_title:Cell proliferation
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doi:10.1046/j.1365-2184.2002.00231.x
更新日期:2002-06-01 00:00:00
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更新日期:2018-06-01 00:00:00
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更新日期:2004-06-01 00:00:00
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journal_title:Cell proliferation
pub_type: 杂志文章
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更新日期:2017-12-01 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2017-04-01 00:00:00
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journal_title:Cell proliferation
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doi:10.1111/cpr.12575
更新日期:2019-05-01 00:00:00
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journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.1992.tb01433.x
更新日期:1992-01-01 00:00:00
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journal_title:Cell proliferation
pub_type: 杂志文章
doi:
更新日期:1997-06-01 00:00:00
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journal_title:Cell proliferation
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doi:10.1111/cpr.12485
更新日期:2018-12-01 00:00:00
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journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.2006.00397.x
更新日期:2006-12-01 00:00:00
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journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.2008.00514.x
更新日期:2008-04-01 00:00:00