Abstract:
OBJECTIVES:The aim of this study was to investigate the role of insulin-like growth factor-1 (IGF-1) and crosstalk between endothelial cells (ECs) and adipose-derived stem cells (ASCs) in the process of angiogenesis. METHODS:A three-dimensional collagen gel used to culture mouse ASCs and mouse ECs in vitro was established. The effects of angiogenesis after exposure to IGF-1 were observed by confocal laser scanning microscopy. Western blotting and qPCR were performed to elucidate the underlying mechanisms. RESULTS:IGF-1 treatment promoted the formation of vessel-like structures and the recruitment of ASCs in the three-dimensional collagen gel. The angiogenic genes and proteins in ECs were up-regulated by IGF-1 and in co-culture. Similar changes in the genes and in the proteins were detected in ASCs after exposure to IGF-1 and co-culture. p-Akt expression levels were high in ECs and ASCs after exposure to IGF-1 and co-culture. CONCLUSIONS:IGF-1 and co-culture between cells facilitate the process of angiogenesis via the PI3-kinase/Akt signalling pathway. In ECs, IGF-1 stimulates the expression of angiogenesis-related growth factors with the activation of the PI3-kinase/Akt signalling pathway. Co-cultured ECs exposed to excess VEGF-A and other angiogenesis-related growth factors para-secreted from ASCs exhibit high expression of angiogenesis-related genes and proteins. In ASCs, IGF-1 induces the recruitment and function of ASCs by up-regulating the expression of PDGFB, MMPs and α-SMA. Crosstalk with ECs further facilitates changes in ASCs.
journal_name
Cell Prolifjournal_title
Cell proliferationauthors
Lin S,Zhang Q,Shao X,Zhang T,Xue C,Shi S,Zhao D,Lin Ydoi
10.1111/cpr.12390subject
Has Abstractpub_date
2017-12-01 00:00:00issue
6eissn
0960-7722issn
1365-2184journal_volume
50pub_type
杂志文章abstract::The human promyelocytic leukaemia cell line, HL-60, was investigated with regard to proliferation and terminal differentiation following irradiation. The cells were X-irradiated and induced with 1.25% dimethyl sulfoxide (DMSO) towards the granulocytic lineage. Proliferation was measured via cell growth, clonogenicity ...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.1993.tb00031.x
更新日期:1993-11-01 00:00:00
abstract:OBJECTIVES:To evaluate the effects of rRNA synthesis inhibition on cell cycle progression and cell population growth according to the RB and p53 status. MATERIAL AND METHODS:RB- and p53-proficient U2OS cells and the RB- and p53-deficient SAOS-2 cells were used, rRNA transcription hindered by actinomycin D, and cell cy...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.2007.00448.x
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doi:10.1111/cpr.12503
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journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.1992.tb01466.x
更新日期:1992-11-01 00:00:00
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journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1046/j.1365-2184.2003.00288.x
更新日期:2003-12-01 00:00:00
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journal_title:Cell proliferation
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doi:10.1111/j.1365-2184.2012.00837.x
更新日期:2012-10-01 00:00:00
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journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12223
更新日期:2015-12-01 00:00:00
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journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.2008.00562.x
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journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.1993.tb00327.x
更新日期:1993-07-01 00:00:00
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journal_title:Cell proliferation
pub_type: 杂志文章,评审
doi:10.1111/cpr.12698
更新日期:2020-01-01 00:00:00
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journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12813
更新日期:2020-07-01 00:00:00
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journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12387
更新日期:2018-02-01 00:00:00
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journal_title:Cell proliferation
pub_type: 杂志文章,评审
doi:10.1111/j.1365-2184.2004.00303.x
更新日期:2004-02-01 00:00:00
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journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.2010.00684.x
更新日期:2010-08-01 00:00:00
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journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.2009.00623.x
更新日期:2009-10-01 00:00:00
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journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.2008.00548.x
更新日期:2008-10-01 00:00:00
abstract:OBJECTIVES:Panax ginseng, a well-known traditional Chinese medicine with multiple pharmacological activities, plays a crucial role in modulating mood disorders. Several recent studies have identified an underlying role of Panax ginseng in the prevention and treatment of depression. However, the cellular and molecular m...
journal_title:Cell proliferation
pub_type: 杂志文章,评审
doi:10.1111/cpr.12696
更新日期:2019-11-01 00:00:00
abstract::Electromagnetic ultra-wideband pulses (UWB) or nanopulses, are generated by a wide range of electronic devices used in communications and radar technology. However, the specific effects of nanopulse exposure on cell growth and function have not been extensively investigated. Here, studies have been conducted to determ...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.2005.00340.x
更新日期:2005-06-01 00:00:00
abstract::Nerous system diseases, both central and peripheral, bring an incredible burden onto patients and enormously reduce their quality of life. Currently, there are still no effective treatments to repair nerve lesions that do not have side effects. Stem cell-based therapies, especially those using dental stem cells, bring...
journal_title:Cell proliferation
pub_type: 杂志文章,评审
doi:10.1111/cpr.12572
更新日期:2019-05-01 00:00:00
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journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12166
更新日期:2015-04-01 00:00:00
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journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12017
更新日期:2013-04-01 00:00:00
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journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.2008.00514.x
更新日期:2008-04-01 00:00:00
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journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.2007.00464.x
更新日期:2007-10-01 00:00:00
abstract::Reports on the p53-related cell cycle and apoptotic responses of EBV-transformed lymphoblastoid cell lines to DNA damage have led to some confusion. This may be due to differences in the nature of the specific p53 mutations under examination, but it can also be partly attributed to methodological and analytical proble...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:
更新日期:1999-02-01 00:00:00
abstract:OBJECTIVES:MicroRNAs are powerful regulators in hepatocellular carcinoma (HCC) tumorigenesis. MicoRNA-191 (miR-191) has been reported to play an important role in HCC, However, the regulatory mechanism is still unclear. In this study, we investigated the role of miR-191 in HCC and studied its underlying mechanisms of a...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12635
更新日期:2019-09-01 00:00:00
abstract::This study examines epithelial cell streaming and turnover in normal rat bulbar conjunctiva. Twenty seven male adult random-bred Hebrew rats weighing between 250-300 g, were injected i.p. with [3H]-thymidine. Three rats were killed at various times, thereafter from 1 h to 28 days. The enucleated eyes were fixed in for...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.1995.tb00066.x
更新日期:1995-04-01 00:00:00
abstract:OBJECTIVES:Worldwide, lung cancer accounts for the majority of cancer-related deaths. Aberrant expression of miRNAs has increasingly been reported to be associated with tumour progression. This study aimed to explore the role of miR-301b in regulating apoptosis in lung cancer. MATERIALS AND METHODS:Expression of miR-3...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12264
更新日期:2016-08-01 00:00:00
abstract::This study focuses on analysis of in vitro cultures of chondrocytes from ovine articular cartilage. Isolated cells were seeded in Petri dishes, then expanded to confluence and phenotypically characterized by flow cytometry. The sigmoidal temporal profile of total counts was obtained by classic haemocytometry and corre...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.2010.00676.x
更新日期:2010-06-01 00:00:00
abstract:OBJECTIVES:Dental follicle cells (DFCs) provide the origin of periodontal tissues, and Runx2 is essential for bone formation and tooth development. In this study, pluripotency of DFCs was evaluated and effects of Runx2 on them were investigated. MATERIALS AND METHODS:The DFCs were induced to differentiate towards oste...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.2010.00670.x
更新日期:2010-06-01 00:00:00
abstract::Natural compounds derived from plant sources are well characterized as possessing a wide variety of remarkable anti-tumour properties, for example modulating programmed cell death, primarily referring to apoptosis, and autophagy. Distinct from apoptosis, autophagy (an evolutionarily conserved, multi-step lysosomal deg...
journal_title:Cell proliferation
pub_type: 杂志文章,评审
doi:10.1111/j.1365-2184.2012.00833.x
更新日期:2012-10-01 00:00:00