Cytometry in the brain: studying differentiation to diagnostic applications in brain disease and regeneration therapy.

abstract：:In situ presence of numerous DNA strand breaks is a typical feature of apoptotic cells. Selective DNA strand break induction by photolysis (SBIP) at sites that contain incorporated halogenated DNA precursors has recently been proposed as a method of analysing DNA replication. Detection of DNA strand breaks, thus, enab...

journal_title：Cell proliferation

authors： Li X,Darzynkiewicz Z

更新日期：1995-11-01 00:00:00

abstract：OBJECTIVES:This study is to explore the role of Notch signalling during the regeneration of rat tracheal epithelium after injury induced by 5-fluorouracil (5-FU). MATERIALS AND METHODS:We developed an ex vivo model of rat tracheal epithelial regeneration using 5-FU to induce injury. Expression levels of members of the...

journal_title：Cell proliferation

authors： Ma XB,Jia XS,Liu YL,Wang LL,Sun SL,Song N,Wang EH,Li F

更新日期：2009-02-01 00:00:00

abstract：:Stem cell research is now a very broad field encompassing cells derived from all stages of life from the embryonic stem cells of the early blastocyst through to the adult stem cells of many tissues of the body. Adult stem cells from a variety of tissues are proving to be pluripotent and can differentiate into cell typ...

journal_title：Cell proliferation

authors： Mackay-Sim A,Silburn P

更新日期：2008-02-01 00:00:00

abstract：:Several methods to synchronize cultured cells in the cell cycle are based on temporary inhibition of DNA replication. Previously it has been reported that cells synchronized this way exhibited significant growth imbalance and unscheduled expression of cyclins A and B1. We have now observed that HL-60 cells exposed to ...

journal_title：Cell proliferation

authors： Kurose A,Tanaka T,Huang X,Traganos F,Darzynkiewicz Z

更新日期：2006-06-01 00:00:00

abstract：OBJECTIVES:The main objective of this paper was to investigate whether the oxygen consumption rate (OCR) of cells entrapped in alginate hydrogels depends on presence of soluble factors present in foetal bovine serum (FBS). MATERIALS AND METHODS:Pericellular oxygen concentrations were measured using a photochemical oxy...

journal_title：Cell proliferation

authors： Larsen BE,Pettersen EO,Tønnesen HH,Melvik JE

更新日期：2015-06-01 00:00:00

abstract：OBJECTIVES:Pre-eclampsia is a leading cause of morbidity and mortality during pregnancy. Although the two forms of this disorder, early- (EOPE) and late-onset of pre-eclampsia (LOPE) are different, the underlying pathology remains elusive. We aim to unravel the difference and to identify novel biomarkers for EOPE and L...

journal_title：Cell proliferation

authors： Guo F,Zhang B,Yang H,Fu Y,Wang Y,Huang J,Cheng M,Li X,Shen Z,Li L,He P,Xiang AP,Wang S,Zhang H

更新日期：2020-12-17 00:00:00

abstract：OBJECTIVE:This study has aimed to study different culture systems that might stimulate an increase in cell proliferation of normal and osteoarthritis chondrocytes from articular cartilage in rat model. MATERIAL AND METHODS:Three culture systems using chondrocytes embedded in alginate beads were tested: chondrocytes cu...

journal_title：Cell proliferation

authors： Gomez-Camarillo MA,Almonte-Becerril M,Vasquez Tort M,Tapia-Ramirez J,Kouri Flores JB

更新日期：2009-04-01 00:00:00

abstract：:Studies to examine the percentages of proliferating plasma cells (PPC) in the Harderian gland (HG) were carried out in chicks between 5 and 12 weeks of age. Two methods, 5-bromo-2'-deoxyuridine (BrdUrd) incorporation into DNA and flow cytometric analysis of propidium iodide (PI) stained cells, were employed in control...

journal_title：Cell proliferation

authors： Savage ML,Oláh I,Scott TR

更新日期：1992-07-01 00:00:00

abstract：:Atherosclerosis is an inflammatory-fibroproliferative response of the arterial wall involving a complex set of interconnected events where cell proliferation (lymphomonocytes, and endothelial and smooth-muscle cells) and substantial perturbations of intracellular cholesterol metabolism are considered to be among the m...

journal_title：Cell proliferation

authors： Batetta B,Bonatesta RR,Sanna F,Putzolu M,Mulas MF,Collu M,Dessì S

更新日期：2002-06-01 00:00:00

abstract：:We have developed a mathematical model based on proliferation and infiltration of neoplastic cells that allows predictions to be made concerning the life expectancies following various extents of surgical resection of gliomas of all grades of malignancy. The key model parameters are the growth rate and the diffusion r...

journal_title：Cell proliferation

authors： Woodward DE,Cook J,Tracqui P,Cruywagen GC,Murray JD,Alvord EC Jr

更新日期：1996-06-01 00:00:00

abstract：:The human promyelocytic leukaemia cell line, HL-60, was investigated with regard to proliferation and terminal differentiation following irradiation. The cells were X-irradiated and induced with 1.25% dimethyl sulfoxide (DMSO) towards the granulocytic lineage. Proliferation was measured via cell growth, clonogenicity ...

journal_title：Cell proliferation

authors： Sendler A,Kaffenberger W,Nuyken I,van Beuningen D

更新日期：1993-11-01 00:00:00

abstract：OBJECTIVES:This study was designed to investigate the role of circ_0078767/miR-330-3p/RASSF1A in non-small-cell lung cancer (NSCLC). Bioinformatic analysis was performed to screen for the differentially expressed genes in NSCLC tissues from adjacent lung tissues. MATERIALS AND METHODS:qRT-PCR was used to detect the RN...

journal_title：Cell proliferation

authors： Chen T,Yang Z,Liu C,Wang L,Yang J,Chen L,Li W

更新日期：2019-03-01 00:00:00

abstract：OBJECTIVES:The aim of this study was to develop functionalized nanofibres as a simple delivery system for growth factors (GFs) and make nanofibre cell-seeded scaffold implants a one-step intervention. MATERIALS AND METHODS:We have functionalized polycaprolactone (PCL) nanofibres with thrombocytes adherent on them. Imm...

journal_title：Cell proliferation

authors： Jakubova R,Mickova A,Buzgo M,Rampichova M,Prosecka E,Tvrdik D,Amler E

更新日期：2011-04-01 00:00:00

abstract：:Studies on growth regulation in vitro to a large extent rely on comparison of growth curves. However, these do not discriminate between the relative contributions of the mitotic rate and the apoptotic rate to the net growth rate. In the present study, differential effects of 17beta-oestradiol (E2, 10(-8) M) and/or tam...

journal_title：Cell proliferation

authors： Budtz PE

更新日期：1999-10-01 00:00:00

abstract：OBJECTIVES:Autophagy, a highly conserved lysosomal degradation process in eukaryotic cells, can digest long-lived proteins and damaged organelles through vesicular trafficking pathways. Nowadays, mechanisms of autophagy have been gradually elucidated and thus the discovery of small-molecule drugs targeting autophagy ha...

journal_title：Cell proliferation

authors： Deng Y,Zhu L,Cai H,Wang G,Liu B

更新日期：2018-06-01 00:00:00

abstract：:Epstein-Barr virus (EBV) infection in vitro causes transformation of B cells and generates B lymphoblastoid cell lines (LCLs). These LCLs have been widely used for the diagnostic of several genetic metabolic disorders. However, up to now, efficiency of LCL generation has been based on misleading subjective analysis. I...

journal_title：Cell proliferation

authors： Mello AS,Burin MG,Michellin K,Viapiana M,Giugliani R,Coelho JC,Bauer ME

更新日期：2006-02-01 00:00:00

abstract：:In the past few years, the paramount role of cancer stem cells (CSCs), in terms of cancer initiation, proliferation, metastasis, invasion and chemoresistance, has been revealed by accumulating studies. However, this level of cellular plasticity cannot be entirely explained by genetic mutations. Research on epigenetic ...

journal_title：Cell proliferation

authors： Zhang S,Gong Y,Li C,Yang W,Li L

更新日期：2020-12-13 00:00:00

abstract：OBJECTIVES:To investigate the role of hierarchical micro/nanoscale topography of direct metal laser sintering (DMLS) titanium surfaces in osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs), as well as the possible underlying epigenetic mechanism. MATERIALS AND METHODS:Three groups of titanium spe...

journal_title：Cell proliferation

authors： Zheng G,Guan B,Hu P,Qi X,Wang P,Kong Y,Liu Z,Gao P,Li R,Zhang X,Wu X,Sui L

更新日期：2018-08-01 00:00:00

abstract：:Recent epidemiological studies have demonstrated a correlation between regular aspirin (acetylsalicylic acid) use and decrease risk for the development of fatal colorectal cancer. An increase in the size of the cell proliferation compartment in colorectal crypts has been correlated with an increased risk for the devel...

journal_title：Cell proliferation

authors： Barnes CJ,Lee M,Hardman WE,Cameron IL

更新日期：1995-02-01 00:00:00

abstract：OBJECTIVES:The aim of this study was to obtain antitumour molecules targeting to activate PKM2 through adequate computational methods combined with biological activity experiments. METHODS:The structure-based virtual screening was utilized to screen effective activator targeting PKM2 from ZINC database. Molecular dyna...

journal_title：Cell proliferation

authors： Li Y,Bao M,Yang C,Chen J,Zhou S,Sun R,Wu C,Li X,Bao J

更新日期：2018-12-01 00:00:00

abstract：OBJECTIVES:It has been widely reported that maternal diabetes impairs oocyte quality. However, the responsible mechanisms remain to be explored. In the present study, we focused on whether SIRT3-GSK3β pathway mediates the meiotic defects in oocytes from diabetic mice. MATERIALS AND METHODS:GSK3β functions in mouse ooc...

journal_title：Cell proliferation

authors： Xin Y,Jin Y,Ge J,Huang Z,Han L,Li C,Wang D,Zhu S,Wang Q

更新日期：2021-01-01 00:00:00

abstract：OBJECTIVES:BCL2 family proteins have been widely studied over the past decade due to their essential roles in apoptosis, oncogenesis and anti-cancer therapy. However, the similarities and differences in the spatial pattern of the BCL2 gene family within the context of chromatin have not been well characterized. We soug...

journal_title：Cell proliferation

authors： Wang J,Li S,Lin S,Fu S,Qiu L,Ding K,Liang K,Du H

更新日期：2020-06-01 00:00:00

abstract：OBJECTIVES:Based on in vivo studies, low-intensity pulsed ultrasound (LIPUS) stimulation has been widely used in the clinic for advancing bone growth during healing of non-union alignment, fractures and other osseous defects. In this study, we have investigated osteogenic differentiation of adipose stem cells (ASCs) re...

journal_title：Cell proliferation

authors： Yue Y,Yang X,Wei X,Chen J,Fu N,Fu Y,Ba K,Li G,Yao Y,Liang C,Zhang J,Cai X,Wang M

更新日期：2013-06-01 00:00:00

abstract：OBJECTIVES:miR-21 can promote osteoblast differentiation of periodontal ligament stem cells. However, the effect of miR-21 on bone remodelling in the midpalatal suture is unclear. This study aimed to elucidate the effects of miR-21 on the midpalatal suture bone remodelling by expanding the palatal sutures. MATERIALS A...

journal_title：Cell proliferation

authors： Li M,Zhang Z,Gu X,Jin Y,Feng C,Yang S,Wei F

更新日期：2020-01-01 00:00:00

abstract：OBJECTIVES:Panax ginseng, a well-known traditional Chinese medicine with multiple pharmacological activities, plays a crucial role in modulating mood disorders. Several recent studies have identified an underlying role of Panax ginseng in the prevention and treatment of depression. However, the cellular and molecular m...

journal_title：Cell proliferation

authors： Jin Y,Cui R,Zhao L,Fan J,Li B

更新日期：2019-11-01 00:00:00

abstract：OBJECTIVES:Histone deacetylase (HDAC) is an important therapeutic target in cancer. Two of the main anticancer mechanisms of HDAC inhibitors are induction of terminal differentiation and inhibition of cell proliferation. To investigate the role of HDAC in maintenance of self-renewal and cell proliferation, we treated m...

journal_title：Cell proliferation

authors： Lee S,Park JR,Seo MS,Roh KH,Park SB,Hwang JW,Sun B,Seo K,Lee YS,Kang SK,Jung JW,Kang KS

更新日期：2009-12-01 00:00:00

abstract：:Breast cancer has seriously been threatening physical and mental health of women in the world, and its morbidity and mortality also show clearly upward trend in China over time. Through inquiry, we find that survival rate of patients with early-stage breast cancer is significantly higher than those with middle- and la...

journal_title：Cell proliferation

authors： He Z,Chen Z,Tan M,Elingarami S,Liu Y,Li T,Deng Y,He N,Li S,Fu J,Li W

更新日期：2020-07-01 00:00:00

abstract：:Autophagy follows a lysosomal degradation pathway in which a cell digests its own components. It is highly regulated by a limited number of autophagy-related genes (Atg) and the proteins they encode, that are crucial for cells to undergo the process via modulating autophagsome formation. Recently, accumulating evidenc...

journal_title：Cell proliferation

authors： Chen Y,Liu XR,Yin YQ,Lee CJ,Wang FT,Liu HQ,Wu XT,Liu J

更新日期：2014-04-01 00:00:00

abstract：OBJECTIVE:Curcumin (capable of inhibiting angiogenic growth of human umbilical vein endothelial cells [HUVECs]), can be employed in vitro as a model of pathogenesis of corneal neovascularization (CRNV). The aim of this study was to explore regulatory mechanisms of microRNA (miR) levels after curcumin treatment. MATERI...

journal_title：Cell proliferation

authors： Bai Y,Wang W,Sun G,Zhang M,Dong J

更新日期：2016-12-01 00:00:00

abstract：OBJECTIVES:Mesenchymal stem cells (MSCs) are frequently used for bone regeneration, however, they are limited in quantity. Moreover, their proliferation and differentiation capabilities reduce during cell culture expansion. Potential application of induced pluripotent stem cells (iPSCs) has been reported as a promising...

journal_title：Cell proliferation

authors： Bastami F,Nazeman P,Moslemi H,Rezai Rad M,Sharifi K,Khojasteh A

更新日期：2017-04-01 00:00:00