Abstract:
OBJECTIVES:Exposure to microgravity induces many adaptive and pathological changes in human bone marrow mesenchymal stem cells (hBMSCs). However, the underlying mechanisms of these changes are poorly understood. We revealed the gene expression patterns of hBMSCs under normal ground (NG) and simulated microgravity (SMG), which showed an interpretation for these changes by gene regulation and signal pathways analysis. MATERIALS AND METHODS:In this study, hBMSCs were osteogenically induced for 0, 2, 7 and 14 days under normal ground gravity and simulated microgravity, followed by analysis of the differences in transcriptome expression during osteogenic differentiation by RNA sequencing and some experimental verification for these results. RESULTS:The results indicated that 837, 399 and 894 differentially expressed genes (DEGs) were identified in 2, 7 and 14 days samples, respectively, out of which 13 genes were selected for qRT-PCR analysis to confirm the RNA-sequencing results. After analysis, we found that proliferation was inhibited in the early stage of induction. In the middle stage, osteogenic differentiation was inhibited, whereas adipogenic differentiation benefited from SMG. Moreover, SMG resulted in the up-regulation of genes specific for tumorigenesis in the later stage. CONCLUSION:Our data revealed that SMG inhibits the proliferation and inhibits the differentiation towards osteoblasts but promotes adipogenesis. SMG also selects highly tumorigenic cells for survival under prolonged SMG.
journal_name
Cell Prolifjournal_title
Cell proliferationauthors
Li L,Zhang C,Chen JL,Hong FF,Chen P,Wang JFdoi
10.1111/cpr.12539subject
Has Abstractpub_date
2019-03-01 00:00:00pages
e12539issue
2eissn
0960-7722issn
1365-2184journal_volume
52pub_type
杂志文章abstract::The expression of the 170-kDa (alpha) and the 180-kDa (beta) isoforms of DNA topoisomerase II (topo II) was investigated with two specific monoclonal antibodies (MoAbs) in human peripheral blood lymphocytes (PBL), before and after phytohaemoagglutinin (PHA) stimulation. Binding of each MoAb was detected by indirect im...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.1994.tb01423.x
更新日期:1994-05-01 00:00:00
abstract:OBJECTIVES:To investigate the role of hierarchical micro/nanoscale topography of direct metal laser sintering (DMLS) titanium surfaces in osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs), as well as the possible underlying epigenetic mechanism. MATERIALS AND METHODS:Three groups of titanium spe...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12460
更新日期:2018-08-01 00:00:00
abstract:OBJECTIVES:This study was designed to investigate the role of circ_0078767/miR-330-3p/RASSF1A in non-small-cell lung cancer (NSCLC). Bioinformatic analysis was performed to screen for the differentially expressed genes in NSCLC tissues from adjacent lung tissues. MATERIALS AND METHODS:qRT-PCR was used to detect the RN...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12548
更新日期:2019-03-01 00:00:00
abstract:OBJECTIVES:Secondary bacterial pneumonia is common following influenza infection. However, it remains unclear about the underlying molecular mechanisms. MATERIALS AND METHODS:We established a mouse model of post-influenza S aureus pneumonia using conditional Shp2 knockout mice (LysMCre/+ :Shp2flox/flox ). The survival...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12721
更新日期:2020-01-01 00:00:00
abstract:OBJECTIVES:This study is to explore the role of Notch signalling during the regeneration of rat tracheal epithelium after injury induced by 5-fluorouracil (5-FU). MATERIALS AND METHODS:We developed an ex vivo model of rat tracheal epithelial regeneration using 5-FU to induce injury. Expression levels of members of the...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.2008.00569.x
更新日期:2009-02-01 00:00:00
abstract:OBJECTIVE:Accumulating data show that dysregulation of long noncoding RNAs (lncRNAs) acts a critical role in a variety of malignancies. Among these lncRNAs, small nucleolar RNA host genes (SNHGs) are associated with tumour growth and progression. But, the molecular mechanisms by which SNHG4 contributes to osteosarcoma ...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12515
更新日期:2018-12-01 00:00:00
abstract:OBJECTIVES:The aim of this study was to investigate the role of insulin-like growth factor-1 (IGF-1) and crosstalk between endothelial cells (ECs) and adipose-derived stem cells (ASCs) in the process of angiogenesis. METHODS:A three-dimensional collagen gel used to culture mouse ASCs and mouse ECs in vitro was establi...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12390
更新日期:2017-12-01 00:00:00
abstract:OBJECTIVES:Accumulating evidence demonstrated that the long noncoding RNA (lncRNA) HOTAIR (Hox transcript antisense intergenic RNA) plays key role in renal cell carcinoma (RCC) malignancy, while microRNA-124 (miR-124) is a tumour suppressor in RCC. The aim of this work was to assess the biological function of HOTAIR an...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12507
更新日期:2018-12-01 00:00:00
abstract:OBJECTIVE:Management of chondral defects has long been a challenge due to poor self-healing capacity of articular cartilage. Many approaches, ranging from symptomatic treatment to structural cartilage regeneration, have obtained very limited satisfactory results. Cartilage tissue engineering, which involves optimized c...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12295
更新日期:2016-12-01 00:00:00
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journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12035
更新日期:2013-06-01 00:00:00
abstract::Proliferation and multidrug resistance status are key predictors of therapeutic outcome in acute myeloid leukaemia (AML). Anthracyclines such as daunorubicin (DNR) are typically used to treat AML and can induce drug resistance. The goal of the studies described here was to select a combination of fluorescent probes th...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1046/j.1365-2184.1999.3240203.x
更新日期:1999-08-01 00:00:00
abstract:OBJECTIVES:Vascular disorders are associated with phenotypical switching of vascular smooth muscle cells (VSMCs). We investigated the effect of bone morphogenetic protein (BMP)-2 in controlling VSMC phenotype and vascular disorder progression. Lysine (K)-specific demethylase 1A (KDM1A) has been identified to target BMP...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12711
更新日期:2020-01-01 00:00:00
abstract::A model of intestinal crypt organization is suggested based on the assumption that stem cells have a finite replicative life span. The model assumes the existence in a crypt of a quiescent ('deep') stem cell and a few more actively cycling ('proximate') stem cells. Monte Carlo computer simulation of published intestin...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.2006.00395.x
更新日期:2006-10-01 00:00:00
abstract::Studies to examine the percentages of proliferating plasma cells (PPC) in the Harderian gland (HG) were carried out in chicks between 5 and 12 weeks of age. Two methods, 5-bromo-2'-deoxyuridine (BrdUrd) incorporation into DNA and flow cytometric analysis of propidium iodide (PI) stained cells, were employed in control...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.1992.tb01444.x
更新日期:1992-07-01 00:00:00
abstract:OBJECTIVES:Worldwide, lung cancer accounts for the majority of cancer-related deaths. Aberrant expression of miRNAs has increasingly been reported to be associated with tumour progression. This study aimed to explore the role of miR-301b in regulating apoptosis in lung cancer. MATERIALS AND METHODS:Expression of miR-3...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12264
更新日期:2016-08-01 00:00:00
abstract:OBJECTIVES:To assess the expression, prognostic value, and functionality of T-lymphokine-activated killer (T-LAK) cell-originated protein kinase (TOPK) in chordoma pathogenesis. MATERIALS AND METHODS:TOPK expression in chordoma was assessed via immunohistochemical staining of a tissue microarray (TMA) and correlated w...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12901
更新日期:2020-10-01 00:00:00
abstract::Several methods to synchronize cultured cells in the cell cycle are based on temporary inhibition of DNA replication. Previously it has been reported that cells synchronized this way exhibited significant growth imbalance and unscheduled expression of cyclins A and B1. We have now observed that HL-60 cells exposed to ...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.2006.00380.x
更新日期:2006-06-01 00:00:00
abstract::RAIDD (RIP-associated ICH-1 homologous protein with a death domain) is an adaptor molecule that mediates the action of cysteine proteases involved in apoptosis. To study the possibility of a novel system of cell ablation mediated by RAIDD, a preadipocyte cell line (3T3L1) was stably transfected with a plasmid containi...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1046/j.1365-2184.2003.00253.x
更新日期:2003-02-01 00:00:00
abstract::WR-1065 (2-[(aminopropyl)amino]ethanethiol) reduces cytotoxic and mutagenic effects caused by exposure of cells to radiation and chemotherapeutic drugs, but the mechanisms involved are not fully known. We have observed an accumulation of cells in G2 in WR-1065 treated Chinese hamster ovary cells grown in alpha-minimal...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.1992.tb01466.x
更新日期:1992-11-01 00:00:00
abstract::Extracellular vesicles (EVs), a heterogeneous group of vesicles differing in size and shape, cargo content and function, are membrane-bound and nano-sized vesicles that could be released by nearly all variations of cells. EVs have gained considerable attention in the past decades for their functions in modulating inte...
journal_title:Cell proliferation
pub_type: 杂志文章,评审
doi:10.1111/cpr.12659
更新日期:2019-11-01 00:00:00
abstract::The validity of the use of the monoclonal antibodies Ki-67 and anti-BrdUrd to evaluate proliferative activity of human prostate tumour models was studied. Growth of the transplantable PC-82 and PC-EW prostate tumours, as assessed by tumour volume measurements, was significantly correlated with the proliferative activi...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.1993.tb00007.x
更新日期:1993-01-01 00:00:00
abstract::Epstein-Barr virus (EBV) infection in vitro causes transformation of B cells and generates B lymphoblastoid cell lines (LCLs). These LCLs have been widely used for the diagnostic of several genetic metabolic disorders. However, up to now, efficiency of LCL generation has been based on misleading subjective analysis. I...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.2006.00363.x
更新日期:2006-02-01 00:00:00
abstract::A number of techniques, including autoradiography after in vivo administration of tritiated thymidine ([3H]dT), immunohistochemistry after in vivo administration of bromodeoxyuridine (BrdUrd), and flow cytometry (FCM) with and without BrdUrd detection were compared in the epithelium of ventral mouse tongue. Investigat...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.1994.tb01429.x
更新日期:1994-06-01 00:00:00
abstract::The R- cell line is a 3T3-like cell line originating from mouse embryos with a homozygous disruption of the type 1 insulin-like growth factor receptor (IGF-IR) genes. Although R- cells cannot grow at all in serum-free medium (SFM) supplemented by several known growth factors, either singly or in combination, they are ...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:
更新日期:1997-06-01 00:00:00
abstract::The microenvironmental changes in the bone marrow, spleen and liver during progression of the transplantable promyelocytic leukaemia in the Brown Norwegian rat (BNML) have been studied. We used flow cytometry to estimate cellular hypoxia and proliferation based on in vivo pulse-labelling with a mixture of 2-nitroimida...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1046/j.1365-2184.2000.00183.x
更新日期:2000-12-01 00:00:00
abstract:OBJECTIVES:This study investigated effects of reduced serum condition and vascular endothelial growth factor (VEGF) on angiogenic potential of adipose stromal cells (ASCs) in vitro. MATERIALS AND METHODS:Adipose stromal cells were cultured in three different types of medium: (i) F12/DMEM (FD) supplemented with 10% FBS...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12029
更新日期:2013-06-01 00:00:00
abstract:OBJECTIVES:In order to provide a more comprehensive understanding of the effects of SARS-CoV-2 on oral health and possible saliva transmission, we performed RNA-seq profiles analysis from public databases and also a questionnaire survey on oral-related symptoms of COVID-19 patients. MATERIALS AND METHODS:To analyse AC...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12923
更新日期:2020-12-01 00:00:00
abstract:OBJECTIVE:Low levels of adiponectin (APN), a biomarker of diabetes mellitus, have been implicated in the poor outcome of intracerebral haemorrhage (ICH). Herein, we aimed to demonstrate the neuroprotective effects of a blood-brain barrier-permeable APN peptide (APNp) on ICH injury in diabetic mice and explore the under...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12759
更新日期:2020-02-01 00:00:00
abstract:OBJECTIVES:This study aims to explore the roles of N-myc and caspase-8 in TRAIL-resistant IMR-32 cells which exhibit MYCN oncogene amplification and lack caspase-8 expression. MATERIALS AND METHODS:We established N-myc-downregulated IMR-32 cells using shRNA lentiviral particles targeting N-myc and examined the effect ...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12577
更新日期:2019-05-01 00:00:00
abstract::Estimates of the clonogen content (number of microcolony-forming cells) of murine intestinal crypts using microcolony assays show an apparent dependence on the radiation dose used in the assay of clonogen content. Crypt radiation survival curves often show increased curvature beyond that expected on the basis of the c...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1046/j.1365-2184.2003.00279.x
更新日期:2003-08-01 00:00:00