Abstract:
OBJECTIVES:Translational research using adult stem cells derived from various tissues has been highlighted in cell-based therapy. However, there are many limitations to using conventional culture systems of adult stem cells for clinically applicability, including limited combinations of cytokines and use of nutrients derived from animals. Here, we have investigated the effects of placental extract (PE) for culture of placenta-derived stem cells (PDSCs) as well as their potential for hepatogenic differentiation. MATERIALS AND METHODS:Placental extract, extracted using water-soluble methods, was used as a supplement for culture of PDSCs. Cell viability was determined using the MTT assay, and cytokine assay was performed using Luminex assay kit. Gene expression, indocyanine green (ICG) up-take, PAS (Periodic Acid-Schiff) staining and urea production were also analysed. RESULTS:The placental extract contained several types of cytokine and chemokine essential for maintenance and differentiation of stem cells. Expression of stemness markers in PDSCs cultured with PE is no different from that of PDSCs cultured with foetal bovine serum (FBS). After hepatogenic differentiation, expression patterns for hepatocyte-specific markers in PDSCs cultured with PE were consistent and potential for hepatogenic differentiation of PDSCs cultured with PE was similar to that of PDSCs cultured with FBS, as shown by PAS staining and urea production assays. CONCLUSIONS:Our findings revealed that placental extract could be used as a new component for culture of adult stem cells, as well as for development of human-based medium, in translational research for regenerative medicine.
journal_name
Cell Prolifjournal_title
Cell proliferationauthors
Shin KS,Lee HJ,Jung J,Cha DH,Kim GJdoi
10.1111/j.1365-2184.2010.00693.xsubject
Has Abstractpub_date
2010-10-01 00:00:00pages
435-44issue
5eissn
0960-7722issn
1365-2184pii
CPR693journal_volume
43pub_type
杂志文章abstract:OBJECTIVES:To understand if there exists a functional interaction between arsenic trioxide and paclitaxel in vitro. MATERIALS AND METHODS:HeLa and HCT116 (rho53(+/+) and rho53(-/-)) cells were treated with As2O3 and/or paclitaxel for various times. Treated cells were collected for analyses using a combination of flow ...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.2009.00606.x
更新日期:2009-06-01 00:00:00
abstract::Nerous system diseases, both central and peripheral, bring an incredible burden onto patients and enormously reduce their quality of life. Currently, there are still no effective treatments to repair nerve lesions that do not have side effects. Stem cell-based therapies, especially those using dental stem cells, bring...
journal_title:Cell proliferation
pub_type: 杂志文章,评审
doi:10.1111/cpr.12572
更新日期:2019-05-01 00:00:00
abstract:OBJECTIVES:Oestrogen receptor (ER) is a common nucleus receptor that is essential for the regulation of cell growth, proliferation and differentiation. This study was to examine whether ERα can affect the proliferation and odonto/osteogenic differentiation of stem cells from apical papilla (SCAPs). MATERIALS AND METHO...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12485
更新日期:2018-12-01 00:00:00
abstract::The R- cell line is a 3T3-like cell line originating from mouse embryos with a homozygous disruption of the type 1 insulin-like growth factor receptor (IGF-IR) genes. Although R- cells cannot grow at all in serum-free medium (SFM) supplemented by several known growth factors, either singly or in combination, they are ...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:
更新日期:1997-06-01 00:00:00
abstract:INTRODUCTION/OBJECTIVES:The serine/threonine kinase homeodomain-interacting protein kinase 2 (HIPK2) is a co-regulator of an increasing number of transcription factors and cofactors involved in DNA damage response and development. We and others have cloned HIPK2 as an interactor of the p53 oncosuppressor, and have stud...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.2009.00601.x
更新日期:2009-06-01 00:00:00
abstract:OBJECTIVE:Curcumin (capable of inhibiting angiogenic growth of human umbilical vein endothelial cells [HUVECs]), can be employed in vitro as a model of pathogenesis of corneal neovascularization (CRNV). The aim of this study was to explore regulatory mechanisms of microRNA (miR) levels after curcumin treatment. MATERI...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12289
更新日期:2016-12-01 00:00:00
abstract:OBJECTIVES:Recent data show that Imatinib mesylate (IM) also affects haematopoietic stem cells (HSC), T lymphocytes and dendritic cells that do not harbour constitutively active tyrosine kinases. MATERIALS AND METHODS:We evaluated possible effects of IM on human bone marrow-derived mesenchymal stem cells (MSC) in vitr...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.2007.00438.x
更新日期:2007-06-01 00:00:00
abstract::Estimates of the clonogen content (number of microcolony-forming cells) of murine intestinal crypts using microcolony assays show an apparent dependence on the radiation dose used in the assay of clonogen content. Crypt radiation survival curves often show increased curvature beyond that expected on the basis of the c...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1046/j.1365-2184.2003.00279.x
更新日期:2003-08-01 00:00:00
abstract:OBJECTIVES:Osteopetrosis is a rare inherited skeletal disease characterized by increased bone mineral density due to the loss of osteoclast function or differentiation potential. MATERIALS AND METHODS:The study involved a Chinese patient with osteopetrosis (the proband) and her immediate family members and 180 control...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12693
更新日期:2019-11-01 00:00:00
abstract::In the past few years, the paramount role of cancer stem cells (CSCs), in terms of cancer initiation, proliferation, metastasis, invasion and chemoresistance, has been revealed by accumulating studies. However, this level of cellular plasticity cannot be entirely explained by genetic mutations. Research on epigenetic ...
journal_title:Cell proliferation
pub_type: 杂志文章,评审
doi:10.1111/cpr.12963
更新日期:2020-12-13 00:00:00
abstract:OBJECTIVES:Often, non-small cell lung cancers (NSCLC) respond only poorly to the tyrosine kinase inhibitor (TKI) gefitinib, which targets the epidermal growth factor receptor (EGFR), these poor responders EGFRs lacking activating mutations. In this study, we have attempted to improve TKI response of NSCLC cell lines (A...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12040
更新日期:2013-08-01 00:00:00
abstract:OBJECTIVES:To evaluate the rapid repair potential of adipose-derived stem cells (ADSCs) co-overexpressing VEGF and GDNF on bilateral cavernous nerve injury (BCNI) in rat models. Progressive fibrosis of the penis that occurs shortly after BCNI is a key cause of clinical treatment difficulty of erectile dysfunction (ED)....
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12756
更新日期:2020-02-01 00:00:00
abstract:OBJECTIVES:Exposure to microgravity induces many adaptive and pathological changes in human bone marrow mesenchymal stem cells (hBMSCs). However, the underlying mechanisms of these changes are poorly understood. We revealed the gene expression patterns of hBMSCs under normal ground (NG) and simulated microgravity (SMG)...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12539
更新日期:2019-03-01 00:00:00
abstract:OBJECTIVES:MicroRNAs are powerful regulators in hepatocellular carcinoma (HCC) tumorigenesis. MicoRNA-191 (miR-191) has been reported to play an important role in HCC, However, the regulatory mechanism is still unclear. In this study, we investigated the role of miR-191 in HCC and studied its underlying mechanisms of a...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12635
更新日期:2019-09-01 00:00:00
abstract:OBJECTIVES:Accumulating studies have investigated the PM2.5-induced pulmonary toxicity, while gaps still remain in understanding its toxic mechanism. Due to its high specific surface area and adsorption capacity similar to nanoparticles, PM2.5 acts as a significant carrier of metals in air and then leads to altered tox...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12813
更新日期:2020-07-01 00:00:00
abstract:OBJECTIVES:Skin-derived precursors are recognized to be a potentially autologous and accessible source of neural precursor cells for drug screening or cell-based treatments, in many neurological disorders. Thus, it is necessary to investigate appropriate methods for cryopreservation of such human skin-derived precursor...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.2011.00803.x
更新日期:2012-04-01 00:00:00
abstract::During brain development, a population of uniform embryonic cells migrates and differentiates into a large number of neural phenotypes - origin of the enormous complexity of the adult nervous system. Processes of cell proliferation, differentiation and programmed death of no longer required cells, do not occur only du...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12087
更新日期:2014-02-01 00:00:00
abstract:OBJECTIVES:Somatic stem cells can be obtained from a variety of adult human tissues. However, it was not clear whether human parathyroid glands, which secrete parathyroid hormones and are essential in maintaining homeostasis levels of calcium ions in the circulation, contained stem cells. We aimed to investigate the po...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.2009.00614.x
更新日期:2009-08-01 00:00:00
abstract::The cell cycle is controlled by numerous mechanisms ensuring correct cell division. This review will focus on these mechanisms, i.e. regulation of cyclin-dependent kinases (CDK) by cyclins, CDK inhibitors and phosphorylating events. The quality checkpoints activated after DNA damage are also discussed. The complexity ...
journal_title:Cell proliferation
pub_type: 杂志文章,评审
doi:10.1046/j.1365-2184.2003.00266.x
更新日期:2003-06-01 00:00:00
abstract:OBJECTIVES:Ubiquitin specific protease 32 (USP32) is a highly conserved but uncharacterized gene, which has been reported to be associated with growth of breast cancer cells. However, the role of USP32 in human small cell lung cancer (SCLC) has not been uncovered. The aim of this study was to investigate and evaluate t...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12343
更新日期:2017-08-01 00:00:00
abstract:OBJECTIVES:It has been widely reported that maternal diabetes impairs oocyte quality. However, the responsible mechanisms remain to be explored. In the present study, we focused on whether SIRT3-GSK3β pathway mediates the meiotic defects in oocytes from diabetic mice. MATERIALS AND METHODS:GSK3β functions in mouse ooc...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12940
更新日期:2021-01-01 00:00:00
abstract::The prostate gland is the site of the most commonly diagnosed cancer in men in USA and UK, accounting for one in five of new cases of male cancer. Common with many other cancer types, prostate cancer is believed to arise from a stem cell that shares characteristics with the normal stem cell. Normal prostate epithelial...
journal_title:Cell proliferation
pub_type: 杂志文章,评审
doi:10.1111/j.1365-2184.2005.00356.x
更新日期:2005-12-01 00:00:00
abstract:OBJECTIVES:The bone tissue engineering primarily focuses on three-dimensional co-culture systems, which physical and biological properties resemble the cell matrix of actual tissues. The complex dialogue between bone-forming and endothelial cells (ECs) in a tissue-engineered construct will directly regulate angiogenesi...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12658
更新日期:2019-09-01 00:00:00
abstract::The proliferation rate of mammalian cells is regulated normally in the G1 phase of the cell cycle. During this phase, it is convenient to assign positive and negative roles to the molecular programs that regulate the duration of G1 and the phase transition from G1 to S phase. Density-dependent inhibition of cellular p...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.1995.tb00089.x
更新日期:1995-09-01 00:00:00
abstract::Reports on the p53-related cell cycle and apoptotic responses of EBV-transformed lymphoblastoid cell lines to DNA damage have led to some confusion. This may be due to differences in the nature of the specific p53 mutations under examination, but it can also be partly attributed to methodological and analytical proble...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:
更新日期:1999-02-01 00:00:00
abstract:OBJECTIVES:Cancer stem cells (CSCs) compose a subpopulation of cells within a tumour that can self-renew and proliferate. Growth factors such as epidermal growth factor (EGF) and basic fibroblast growth factor (b-FGF) promote cancer stem cell proliferation in many solid tumours. This study assesses whether EGF, bFGF an...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.2012.00837.x
更新日期:2012-10-01 00:00:00
abstract::The epithelium of the oral cavity and small intestine of the gastrointestinal tract have a high rate of cell renewal and as such, are sensitive to cytotoxic therapies that kill rapidly dividing cells. Mucositis is a complication of cancer therapy where impairment of the regenerative capacity of the epithelium leads to...
journal_title:Cell proliferation
pub_type: 杂志文章,评审
doi:10.1046/j.1365-2184.35.s1.8.x
更新日期:2002-08-01 00:00:00
abstract::Cell proliferation was investigated in human tumour xenografts using bromodeoxyuridine (BrdUrd) labelling, evaluated either by flow cytometry or in tissue sections, and also using the proliferation marker Ki-67. BrdUrd labelling was found to increase when cryostat tumour sections were digested with an enzymic solution...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.1991.tb01147.x
更新日期:1991-03-01 00:00:00
abstract:OBJECTIVES:Silicate bioactive glass (BG) has been widely demonstrated to stimulate both of the hard and soft tissue regeneration, in which ion products released from BG play important roles. However, the mechanism by which ion products act on cells on cells is unclear. MATERIALS AND METHODS:Human umbilical vein endoth...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12906
更新日期:2020-11-01 00:00:00
abstract:OBJECTIVE:Low levels of adiponectin (APN), a biomarker of diabetes mellitus, have been implicated in the poor outcome of intracerebral haemorrhage (ICH). Herein, we aimed to demonstrate the neuroprotective effects of a blood-brain barrier-permeable APN peptide (APNp) on ICH injury in diabetic mice and explore the under...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12759
更新日期:2020-02-01 00:00:00