Measurement of cell proliferation by enzyme-linked immunosorbent assay (ELISA) using a monoclonal antibody to bromodeoxyuridine.

Abstract:

:An ELISA was developed and optimized to measure cell proliferation using a monoclonal antibody to bromodeoxyuridine (BrdUrd). Incorporation of BrdUrd into myoblast monolayers, measured as the optical density at 492 nm, increased in response to fetal calf serum, IGF-I and EGF, the ELISA data correlated closely with data obtained by BrdUrd immunocytochemistry (r = 0.984), cell counting (r = 0.972) and tritiated thymidine uptake by liquid scintillation counting (r = 0.990). The BrdUrd ELISA is a useful alternative to measurement of tritiated thymidine uptake by scintillation counting, and has the added advantages of dispensing with the use of radioactivity and of being less labour intensive.

journal_name

Cell Prolif

journal_title

Cell proliferation

authors

Perros P,Weightman DR

doi

10.1111/j.1365-2184.1991.tb01179.x

subject

Has Abstract

pub_date

1991-09-01 00:00:00

pages

517-23

issue

5

eissn

0960-7722

issn

1365-2184

journal_volume

24

pub_type

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