Abstract:
:An ELISA was developed and optimized to measure cell proliferation using a monoclonal antibody to bromodeoxyuridine (BrdUrd). Incorporation of BrdUrd into myoblast monolayers, measured as the optical density at 492 nm, increased in response to fetal calf serum, IGF-I and EGF, the ELISA data correlated closely with data obtained by BrdUrd immunocytochemistry (r = 0.984), cell counting (r = 0.972) and tritiated thymidine uptake by liquid scintillation counting (r = 0.990). The BrdUrd ELISA is a useful alternative to measurement of tritiated thymidine uptake by scintillation counting, and has the added advantages of dispensing with the use of radioactivity and of being less labour intensive.
journal_name
Cell Prolifjournal_title
Cell proliferationauthors
Perros P,Weightman DRdoi
10.1111/j.1365-2184.1991.tb01179.xsubject
Has Abstractpub_date
1991-09-01 00:00:00pages
517-23issue
5eissn
0960-7722issn
1365-2184journal_volume
24pub_type
杂志文章abstract::Rabbit embryo-fetal fluid (EFF) contains regulatory factors of cell proliferation which increase the duration of the cell cycle, induce a quiescent status in some cells and lead up to cell death in others. The objective of this study was to demonstrate which of the two processes, namely necrosis or apoptosis, was resp...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.1993.tb00013.x
更新日期:1993-03-01 00:00:00
abstract:OBJECTIVES:Intracellular reactive oxygen species (ROS) induced by receptor activator of NF-kB ligand (RANKL) has been proven to be a critical factor in the development of osteoclasts. This study aimed to prove that schisandrin A (Sch), a novel anti-oxidant compound, is able to suppress osteoclastogenesis and prevent bo...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12882
更新日期:2020-10-01 00:00:00
abstract::A mathematical model for the cell cycle is proposed that incorporates the known biochemical reactions involving both cyclin A and cyclin B, the interactions of these cyclins with cdc2 and cdk2, and the controlling effects of cdc25 and weel. The model also postulates the existence of an as yet unknown phosphatase invol...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.1994.tb01409.x
更新日期:1994-02-01 00:00:00
abstract:OBJECTIVES:Postflight orthostatic intolerance has been regarded as a major adverse effect after microgravity exposure, in which cerebrovascular adaptation plays a critical role. Our previous finding suggested that dedifferentiation of vascular smooth muscle cells (VSMCs) might be one of the key contributors to cerebrov...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12774
更新日期:2020-03-01 00:00:00
abstract:OBJECTIVES:Vascular disorders are associated with phenotypical switching of vascular smooth muscle cells (VSMCs). We investigated the effect of bone morphogenetic protein (BMP)-2 in controlling VSMC phenotype and vascular disorder progression. Lysine (K)-specific demethylase 1A (KDM1A) has been identified to target BMP...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12711
更新日期:2020-01-01 00:00:00
abstract:OBJECTIVES:During human pregnancy, the endothelial cells of the uterine spiral arteries (SPA) are extensively replaced by a subtype of placental trophoblasts, endovascular extravillous trophoblasts (enEVTs), thus establishing a placental-maternal circulation. On this pathway, foetus-derived placental villi and enEVTs b...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12802
更新日期:2020-05-01 00:00:00
abstract:OBJECTIVES:Nonylphenol (NP) is present ubiquitously in both aquatic and terrestrial environments. This compound is considered an important endocrine disruptor and its toxic/oestrogenic activity has been investigated in a number of in vitro and in vivo studies. Human exposure to NP may occur by cutaneous absorption, ing...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.2011.00774.x
更新日期:2011-10-01 00:00:00
abstract:OBJECTIVES:Restoring a functional beta-cell mass is a fundamental goal in treating diabetes. A complex signalling pathway network coordinates the regulation of beta-cell proliferation, although a role for ERK5 in this network has not been reported. This question was addressed in this study. MATERIALS AND METHODS:We st...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12410
更新日期:2018-06-01 00:00:00
abstract::Sertoli cells (SeC) are responsible for the immunoprivileged status of the testis thanks to which allogeneic or xenogeneic engraftments can survive without pharmacological immune suppression if co-injected with SeC. This peculiar ability of SeC is dependent on secretion of a plethora of factors including maturation fa...
journal_title:Cell proliferation
pub_type: 杂志文章,评审
doi:10.1111/cpr.12599
更新日期:2019-05-01 00:00:00
abstract:OBJECTIVES:MicroRNAs are powerful regulators in hepatocellular carcinoma (HCC) tumorigenesis. MicoRNA-191 (miR-191) has been reported to play an important role in HCC, However, the regulatory mechanism is still unclear. In this study, we investigated the role of miR-191 in HCC and studied its underlying mechanisms of a...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12635
更新日期:2019-09-01 00:00:00
abstract::During brain development, a population of uniform embryonic cells migrates and differentiates into a large number of neural phenotypes - origin of the enormous complexity of the adult nervous system. Processes of cell proliferation, differentiation and programmed death of no longer required cells, do not occur only du...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12087
更新日期:2014-02-01 00:00:00
abstract:OBJECTIVES:Osteopetrosis is a rare inherited skeletal disease characterized by increased bone mineral density due to the loss of osteoclast function or differentiation potential. MATERIALS AND METHODS:The study involved a Chinese patient with osteopetrosis (the proband) and her immediate family members and 180 control...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12693
更新日期:2019-11-01 00:00:00
abstract:OBJECTIVE:This study has aimed to study different culture systems that might stimulate an increase in cell proliferation of normal and osteoarthritis chondrocytes from articular cartilage in rat model. MATERIAL AND METHODS:Three culture systems using chondrocytes embedded in alginate beads were tested: chondrocytes cu...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.2008.00580.x
更新日期:2009-04-01 00:00:00
abstract::Reports on the p53-related cell cycle and apoptotic responses of EBV-transformed lymphoblastoid cell lines to DNA damage have led to some confusion. This may be due to differences in the nature of the specific p53 mutations under examination, but it can also be partly attributed to methodological and analytical proble...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:
更新日期:1999-02-01 00:00:00
abstract:OBJECTIVES:To investigate the role of hierarchical micro/nanoscale topography of direct metal laser sintering (DMLS) titanium surfaces in osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs), as well as the possible underlying epigenetic mechanism. MATERIALS AND METHODS:Three groups of titanium spe...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12460
更新日期:2018-08-01 00:00:00
abstract::Natural compounds derived from plant sources are well characterized as possessing a wide variety of remarkable anti-tumour properties, for example modulating programmed cell death, primarily referring to apoptosis, and autophagy. Distinct from apoptosis, autophagy (an evolutionarily conserved, multi-step lysosomal deg...
journal_title:Cell proliferation
pub_type: 杂志文章,评审
doi:10.1111/j.1365-2184.2012.00833.x
更新日期:2012-10-01 00:00:00
abstract:OBJECTIVES:Mesenchymal stem cells (MSCs) are frequently used for bone regeneration, however, they are limited in quantity. Moreover, their proliferation and differentiation capabilities reduce during cell culture expansion. Potential application of induced pluripotent stem cells (iPSCs) has been reported as a promising...
journal_title:Cell proliferation
pub_type: 杂志文章,评审
doi:10.1111/cpr.12321
更新日期:2017-04-01 00:00:00
abstract::Intra-erythrocytic Plasmodium species can be stained with the DNA binding dye, Hoechst 33342, and the distribution of DNA content determined for parasite populations by flow cytometric measurement of fluorescence. Analysis of this distribution will determine the parasitaemia (percentage of erythrocytes infected), and ...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.1992.tb01452.x
更新日期:1992-09-01 00:00:00
abstract:OBJECTIVES:In order to provide a more comprehensive understanding of the effects of SARS-CoV-2 on oral health and possible saliva transmission, we performed RNA-seq profiles analysis from public databases and also a questionnaire survey on oral-related symptoms of COVID-19 patients. MATERIALS AND METHODS:To analyse AC...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12923
更新日期:2020-12-01 00:00:00
abstract:OBJECTIVES:Yes-associated protein (YAP) has been reported to regulate cell proliferation and differentiation. We aimed to characterize the role of YAP in angiotensin II (Ang II)-induced hypertensive vascular remodelling (HVR) and vascular smooth muscle cells (VSMCs) phenotypic modulation and to explore the underlying m...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12517
更新日期:2018-12-01 00:00:00
abstract::The essential oil extracted from clove (Syzygium aromaticum) is used as a topical application to relieve pain and promote healing in herbal medicine and also finds use in the fragrance and flavouring industries. Clove oil has two major components, eugenol and beta-caryophyllene, which constitute 78% and 13% of the oil...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.2006.00384.x
更新日期:2006-08-01 00:00:00
abstract::The microenvironmental changes in the bone marrow, spleen and liver during progression of the transplantable promyelocytic leukaemia in the Brown Norwegian rat (BNML) have been studied. We used flow cytometry to estimate cellular hypoxia and proliferation based on in vivo pulse-labelling with a mixture of 2-nitroimida...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1046/j.1365-2184.2000.00183.x
更新日期:2000-12-01 00:00:00
abstract:OBJECTIVES:The use of platelets and platelet products has become increasingly popular clinically as a means of accelerating endosseous wound healing. It is likely that growth factors released by activated platelets at the site of injury play a role in periodontal regeneration by regulating cellular activity. The purpos...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.2007.00430.x
更新日期:2007-04-01 00:00:00
abstract:OBJECTIVES:It has been widely reported that maternal diabetes impairs oocyte quality. However, the responsible mechanisms remain to be explored. In the present study, we focused on whether SIRT3-GSK3β pathway mediates the meiotic defects in oocytes from diabetic mice. MATERIALS AND METHODS:GSK3β functions in mouse ooc...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12940
更新日期:2021-01-01 00:00:00
abstract:OBJECTIVES:Glucagon-like peptide 2 (GLP2) is involved in the regulation of energy absorption and metabolism. Despite the importance of the GLP2 signalling mechanisms on osteoclast, little has been studied on how GLP2 works during osteoclastogenesis. MATERIALS AND METHODS:RAW264.7 cells were infected with rLV-Green-GLP...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12443
更新日期:2018-08-01 00:00:00
abstract:OBJECTIVES:Increasing evidences suggest that inducing mesenchymal stem cells to differentiate into osteoblasts has been as an especially important component in the prevention and therapy for degenerative bone disease. Here, we identify a novel lncRNA, linc02349, which increases significantly during osteogenic different...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12814
更新日期:2020-05-01 00:00:00
abstract:OBJECTIVES:Both interleukin-6 (IL-6) and transforming growth factor-beta (TGF-beta) are crucially involved in fibrotic events that characterize interstitial lung diseases (ILD). Therefore, the aim of this study was to investigate in primary cultures of normal and fibrotic human lung fibroblasts (HLF), exposed to either...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.2008.00538.x
更新日期:2008-06-01 00:00:00
abstract:OBJECTIVE:In this study, we generated an Rbm14 knockout mouse model to explore its functions during early mouse embryogenesis. MATERIALS AND METHODS:The Rbm14 knockout mouse model was generated by a combination of clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 and microinjection techniques. Th...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12724
更新日期:2020-01-01 00:00:00
abstract:OBJECTIVES:Creation of functional, durable vasculature remains an important goal within the field of regenerative medicine. Engineered biological vasculature has the potential to restore or improve human tissue function. We hypothesized that the pleotropic effects of insulin-like growth factor 1 (IGF1) would enhance th...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12387
更新日期:2018-02-01 00:00:00
abstract:OBJECTIVES:Enterochromaffin (EC) cells have been associated with functional gastrointestinal disorders such as IBS. Recently, we found that glial cell-derived neurotrophic factor (GDNF)-rearranged during transfection (RET) localized in EC cells in human colonic epithelia. Here, we examine the role of GDNF-RET in the pa...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12889
更新日期:2020-10-01 00:00:00