Abstract:
OBJECTIVES:Enterochromaffin (EC) cells have been associated with functional gastrointestinal disorders such as IBS. Recently, we found that glial cell-derived neurotrophic factor (GDNF)-rearranged during transfection (RET) localized in EC cells in human colonic epithelia. Here, we examine the role of GDNF-RET in the pathophysiology of diarrhoea-predominant irritable bowel syndrome (IBS-D). MATERIALS AND METHODS:GDNF was assessed by ELISA and immunohistochemistry in biopsies from IBS-D patients and healthy controls. Stress was induced by using a wrap-restraint stress (WRS) procedure to serve as an acute stress-induced IBS model. The function of GDNF-RET axis to intestinal stem cell (ISC) homeostasis, and EC cell numbers were assessed in vivo and in vitro. RESULTS:GDNF-RET was expressed in EC cells in human colon. GDNF was significantly increased in IBS-D patients. WRS mice showed increased GDNF-RET levels in colon. WRS induced visceral hypersensitivity by expanding of ISC and differentiation of EC cell via GDNF-RET. Furthermore, GDNF-treated mice recapitulated the phenotype of WRS mice. In vitro, GDNF treatment amplified Wnt signal and increased serotonin levels in colonic organoids in a dose-dependent manner. CONCLUSIONS:We identified GDNF-RET was presented in colonic epithelium of patients with IBS-D. GDNF-RET played important roles in regulating ISC and EC cell differentiation. Our findings, thus, provide RET inhibitor as new therapeutic targets for treatment of patients with IBS-D.
journal_name
Cell Prolifjournal_title
Cell proliferationauthors
Lin L,Feng B,Zhou R,Liu Y,Li L,Wang K,Yu Y,Liu C,Long X,Gu X,Li B,Wang X,Yang X,Cong Y,Zuo X,Li Ydoi
10.1111/cpr.12889subject
Has Abstractpub_date
2020-10-01 00:00:00pages
e12889issue
10eissn
0960-7722issn
1365-2184journal_volume
53pub_type
杂志文章abstract::Proliferation and multidrug resistance status are key predictors of therapeutic outcome in acute myeloid leukaemia (AML). Anthracyclines such as daunorubicin (DNR) are typically used to treat AML and can induce drug resistance. The goal of the studies described here was to select a combination of fluorescent probes th...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1046/j.1365-2184.1999.3240203.x
更新日期:1999-08-01 00:00:00
abstract:OBJECTIVES:Osteosarcoma is the most commonly diagnosed primary malignancy of bone and its overall survival rate is still very low. The molecular mechanisms underlying the progression of osteosarcoma have not been clearly illuminated. Heat shock transcription factor 1 (HSF1) is a key regulator of the heat shock response...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12346
更新日期:2017-08-01 00:00:00
abstract:OBJECTIVES:Intracellular reactive oxygen species (ROS) induced by receptor activator of NF-kB ligand (RANKL) has been proven to be a critical factor in the development of osteoclasts. This study aimed to prove that schisandrin A (Sch), a novel anti-oxidant compound, is able to suppress osteoclastogenesis and prevent bo...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12882
更新日期:2020-10-01 00:00:00
abstract::We previously found that the stable overexpression of oestrogen receptor-alpha in the human endothelial cell line ECV304* inhibits its growth in vitro, and that this inhibition is possibly mediated through a down-regulation of the vasoactive agents endothelin-1 and vascular endothelial growth factor. Here we show an i...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1046/j.0960-7722.2001.00207.x
更新日期:2001-08-01 00:00:00
abstract::The effect of various GnRH analogues, and their conjugates on proliferation, clonogenicity and cell cycle phase distribution of MCF-7 and Ishikawa human cancer cell lines was studied. GnRH-III, a sea lamprey GnRH analogue reduced cell proliferation by 35% and clonogenicity by 55%. Structural modifications either decre...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1046/j.1365-2184.2000.00180.x
更新日期:2000-10-01 00:00:00
abstract:OBJECTIVES:Bone marrow edema is a universal manifestation of rheumatoid arthritis (RA), and its pathological essence is a bone marrow lesion (BML) formed by various bone marrow (BM) immune cells. Neutrophils play an important role in inflammatory arthritis, but the role and mechanism of neutrophils in BML are not clear...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12824
更新日期:2020-07-01 00:00:00
abstract:OBJECTIVES:The use of platelets and platelet products has become increasingly popular clinically as a means of accelerating endosseous wound healing. It is likely that growth factors released by activated platelets at the site of injury play a role in periodontal regeneration by regulating cellular activity. The purpos...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.2007.00430.x
更新日期:2007-04-01 00:00:00
abstract:OBJECTIVES:The transfer of melanosomes from melanocytes to neighbouring keratinocytes is critical to protect the skin from the deleterious effects of ultraviolet A (UVA) and ultraviolet B (UVB) irradiation; however, the initial factor(s) that stimulates melanosome transfer remains unclear. In this study, we investigate...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12372
更新日期:2017-12-01 00:00:00
abstract::The CD133 glycoprotein is a controversial cancer stem cell marker in the field of neuro-oncology, based largely on the now considerable experimental evidence for the existence of both CD133+ve and CD133-ve populations as tumour-initiating cells. It is thought that decreasing oxygen tension enhances the complex regulat...
journal_title:Cell proliferation
pub_type: 杂志文章,评审
doi:10.1111/j.1365-2184.2012.00842.x
更新日期:2012-12-01 00:00:00
abstract::Laminin-2 (LN-2, alpha2beta1gamma1) is a basement membrane-associated laminin isoform usually considered in the context of muscle and nerve tissues. To test the hypothesis that LN-2 can additionally modulate epithelial cell biology, an analysis of the role of LN-2 in cell adhesion, activation of signalling intermediat...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.2004.00292.x
更新日期:2004-04-01 00:00:00
abstract:OBJECTIVES:Worldwide, lung cancer accounts for the majority of cancer-related deaths. Aberrant expression of miRNAs has increasingly been reported to be associated with tumour progression. This study aimed to explore the role of miR-301b in regulating apoptosis in lung cancer. MATERIALS AND METHODS:Expression of miR-3...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12264
更新日期:2016-08-01 00:00:00
abstract::Sertoli cells (SeC) are responsible for the immunoprivileged status of the testis thanks to which allogeneic or xenogeneic engraftments can survive without pharmacological immune suppression if co-injected with SeC. This peculiar ability of SeC is dependent on secretion of a plethora of factors including maturation fa...
journal_title:Cell proliferation
pub_type: 杂志文章,评审
doi:10.1111/cpr.12599
更新日期:2019-05-01 00:00:00
abstract:OBJECTIVES:During human pregnancy, the endothelial cells of the uterine spiral arteries (SPA) are extensively replaced by a subtype of placental trophoblasts, endovascular extravillous trophoblasts (enEVTs), thus establishing a placental-maternal circulation. On this pathway, foetus-derived placental villi and enEVTs b...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12802
更新日期:2020-05-01 00:00:00
abstract::Studies to examine the percentages of proliferating plasma cells (PPC) in the Harderian gland (HG) were carried out in chicks between 5 and 12 weeks of age. Two methods, 5-bromo-2'-deoxyuridine (BrdUrd) incorporation into DNA and flow cytometric analysis of propidium iodide (PI) stained cells, were employed in control...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.1992.tb01444.x
更新日期:1992-07-01 00:00:00
abstract:OBJECTIVES:To investigate the role of hierarchical micro/nanoscale topography of direct metal laser sintering (DMLS) titanium surfaces in osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs), as well as the possible underlying epigenetic mechanism. MATERIALS AND METHODS:Three groups of titanium spe...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12460
更新日期:2018-08-01 00:00:00
abstract::Chronic kidney disease (CKD) is a severe health problem worldwide, and vascular calcification (VC) contributes substantially to the cardiovascular morbidity and high mortality of CKD. CKD is often accompanied by a variety of pathophysiological states, such as inflammation, oxidative stress, hyperglycaemia, hyperparath...
journal_title:Cell proliferation
pub_type: 杂志文章,评审
doi:10.1111/cpr.12980
更新日期:2021-01-27 00:00:00
abstract:OBJECTIVES:Both interleukin-6 (IL-6) and transforming growth factor-beta (TGF-beta) are crucially involved in fibrotic events that characterize interstitial lung diseases (ILD). Therefore, the aim of this study was to investigate in primary cultures of normal and fibrotic human lung fibroblasts (HLF), exposed to either...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.2008.00538.x
更新日期:2008-06-01 00:00:00
abstract::RAIDD (RIP-associated ICH-1 homologous protein with a death domain) is an adaptor molecule that mediates the action of cysteine proteases involved in apoptosis. To study the possibility of a novel system of cell ablation mediated by RAIDD, a preadipocyte cell line (3T3L1) was stably transfected with a plasmid containi...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1046/j.1365-2184.2003.00253.x
更新日期:2003-02-01 00:00:00
abstract:OBJECTIVES:This study focuses on experimental analysis and corresponding mathematical simulation of in vitro HUVECs (human umbilical vein endothelial cells) proliferation in the presence of various types of drugs. MATERIALS AND METHODS:HUVECs, once seeded in Petri dishes, were expanded to confluence. Temporal profiles...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.2010.00711.x
更新日期:2010-12-01 00:00:00
abstract::Rabbit embryo-fetal fluid (EFF) contains regulatory factors of cell proliferation which increase the duration of the cell cycle, induce a quiescent status in some cells and lead up to cell death in others. The objective of this study was to demonstrate which of the two processes, namely necrosis or apoptosis, was resp...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.1993.tb00013.x
更新日期:1993-03-01 00:00:00
abstract:OBJECTIVES:The bone tissue engineering primarily focuses on three-dimensional co-culture systems, which physical and biological properties resemble the cell matrix of actual tissues. The complex dialogue between bone-forming and endothelial cells (ECs) in a tissue-engineered construct will directly regulate angiogenesi...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12658
更新日期:2019-09-01 00:00:00
abstract::Human epidemiological reports and rodent experimental research data indicate a possible chemopreventive effect of regular aspirin use for decreasing risk of colon and rectum cancer incidence and mortality. We have previously demonstrated that aspirin can significantly suppress proliferative parameters in normal rat co...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.1996.tb00989.x
更新日期:1996-08-01 00:00:00
abstract:OBJECTIVES:To evaluate the effects of rRNA synthesis inhibition on cell cycle progression and cell population growth according to the RB and p53 status. MATERIAL AND METHODS:RB- and p53-proficient U2OS cells and the RB- and p53-deficient SAOS-2 cells were used, rRNA transcription hindered by actinomycin D, and cell cy...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.2007.00448.x
更新日期:2007-08-01 00:00:00
abstract::Cytokines and growth factors that take part in the regulation of haematopoietic cell development activate many signalling pathways in target cells. The STAT5 (signal transducers and activators of transcription) proteins are members of a family of signal transducers and activators of transcription that can be activated...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.2004.00308.x
更新日期:2004-06-01 00:00:00
abstract:OBJECTIVES:In peripheral neuropathy, the underlying mechanisms of nerve and muscle degeneration include chronic inflammation and oxidative stress in fibrotic tissues. (-)-Epigallocatechin gallate (EGCG) is a major, active component in green tea and may scavenge free radical oxygen and attenuate inflammation. Conservati...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12730
更新日期:2020-01-01 00:00:00
abstract:OBJECTIVES:The aim of the current study was to investigate whether nicotine treatment would induce the proliferation of isolated rat primary pancreatic acinar cells in culture by activating mitogen-activated protein kinase (MAPK) signalling and exocrine secretion. MATERIALS AND METHODS:A nicotine dose- and time-respon...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.2007.00418.x
更新日期:2007-02-01 00:00:00
abstract::Extracellular vesicles (EVs), a heterogeneous group of vesicles differing in size and shape, cargo content and function, are membrane-bound and nano-sized vesicles that could be released by nearly all variations of cells. EVs have gained considerable attention in the past decades for their functions in modulating inte...
journal_title:Cell proliferation
pub_type: 杂志文章,评审
doi:10.1111/cpr.12659
更新日期:2019-11-01 00:00:00
abstract::Several methods to synchronize cultured cells in the cell cycle are based on temporary inhibition of DNA replication. Previously it has been reported that cells synchronized this way exhibited significant growth imbalance and unscheduled expression of cyclins A and B1. We have now observed that HL-60 cells exposed to ...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.2006.00380.x
更新日期:2006-06-01 00:00:00
abstract:OBJECTIVE:Spinal cord tumours are highly malignant and often lead to paralysis and death due to their infiltrative nature, high recurrence rate and limited treatment options. In this study, we measured antitumour efficacy of the Salmonella typhimurium A1-R tumour-targeting bacterium strain, administered systemically or...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.2009.00652.x
更新日期:2010-02-01 00:00:00
abstract:OBJECTIVES:Pre-eclampsia is a leading cause of morbidity and mortality during pregnancy. Although the two forms of this disorder, early- (EOPE) and late-onset of pre-eclampsia (LOPE) are different, the underlying pathology remains elusive. We aim to unravel the difference and to identify novel biomarkers for EOPE and L...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12968
更新日期:2020-12-17 00:00:00