Abstract:
OBJECTIVES:In peripheral neuropathy, the underlying mechanisms of nerve and muscle degeneration include chronic inflammation and oxidative stress in fibrotic tissues. (-)-Epigallocatechin gallate (EGCG) is a major, active component in green tea and may scavenge free radical oxygen and attenuate inflammation. Conservative treatments such as steroid injection only deal with early, asymptomatic, peripheral neuropathy. In contrast, neurolysis and nerve conduit implantation work effectively for treating advanced stages. MATERIALS AND METHODS:An EGCG-loaded polycaprolactone (PCL) porous scaffold was fabricated using an integrated moulding method. We evaluated proliferative, oxidative and inflammatory activity of rat Schwann cells (RSCs) and rat skeletal muscle cells (RSMCs) cultured on different scaffolds in vitro. In a rat radiation injury model, we assessed the morphological, electrophysiological and functional performance of regenerated sciatic nerves and gastrocnemius muscles, as well as oxidative stress and inflammation state. RESULTS:RSCs and RSMCs exhibited higher proliferative, anti-oxidant and anti-inflammatory states in an EGCG/PCL scaffold. In vivo studies showed improved nerve and muscle recovery in the EGCG/PCL group, with increased nerve myelination and muscle fibre proliferation and reduced macrophage infiltration, lipid peroxidation, inflammation and oxidative stress indicators. CONCLUSIONS:The EGCG-modified PCL porous nerve scaffold alleviates cellular oxidative stress and repairs peripheral nerve and muscle structure in rats. It attenuates oxidative stress and inflammation in vivo and may provide further insights into peripheral nerve repair in the future.
journal_name
Cell Prolifjournal_title
Cell proliferationauthors
Qian Y,Yao Z,Wang X,Cheng Y,Fang Z,Yuan WE,Fan C,Ouyang Ydoi
10.1111/cpr.12730subject
Has Abstractpub_date
2020-01-01 00:00:00pages
e12730issue
1eissn
0960-7722issn
1365-2184journal_volume
53pub_type
杂志文章abstract:OBJECTIVES:The present study aimed to investigate overall effect of quercetin on proliferation and osteogenic differentiation of mouse adipose stem cells (mASCs) in vitro. MATERIALS AND METHODS:Mouse adipose stem cells were isolated from subcutaneous fat pads and induced into the osteogenic lineage. Effects of quercet...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12097
更新日期:2014-04-01 00:00:00
abstract:OBJECTIVES:Cancer stem cells (CSCs) compose a subpopulation of cells within a tumour that can self-renew and proliferate. Growth factors such as epidermal growth factor (EGF) and basic fibroblast growth factor (b-FGF) promote cancer stem cell proliferation in many solid tumours. This study assesses whether EGF, bFGF an...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.2012.00837.x
更新日期:2012-10-01 00:00:00
abstract:OBJECTIVES:BCL2 family proteins have been widely studied over the past decade due to their essential roles in apoptosis, oncogenesis and anti-cancer therapy. However, the similarities and differences in the spatial pattern of the BCL2 gene family within the context of chromatin have not been well characterized. We soug...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12826
更新日期:2020-06-01 00:00:00
abstract::During brain development, a population of uniform embryonic cells migrates and differentiates into a large number of neural phenotypes - origin of the enormous complexity of the adult nervous system. Processes of cell proliferation, differentiation and programmed death of no longer required cells, do not occur only du...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12087
更新日期:2014-02-01 00:00:00
abstract:OBJECTIVES:Induced pluripotent stem cell (iPSC)-derived cardiomyocytes are a promising source of cells for regenerative heart disease therapies, but progress towards their use has been limited by their low differentiation efficiency and high cellular heterogeneity. Previous studies have demonstrated expression of adren...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12310
更新日期:2017-02-01 00:00:00
abstract::Neural stem cells (NSCs) are early precursors of neuronal and glial cells. NSCs are capable of generating identical progeny through virtually unlimited numbers of cell divisions (cell proliferation), producing daughter cells committed to differentiation. Nuclear factor kappa B (NF-kappaB) is an inducible, ubiquitous t...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.2006.00403.x
更新日期:2006-12-01 00:00:00
abstract:OBJECTIVES:Postflight orthostatic intolerance has been regarded as a major adverse effect after microgravity exposure, in which cerebrovascular adaptation plays a critical role. Our previous finding suggested that dedifferentiation of vascular smooth muscle cells (VSMCs) might be one of the key contributors to cerebrov...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12774
更新日期:2020-03-01 00:00:00
abstract:OBJECTIVES:Limbal stem cells (LSC) are self-renewing, highly proliferative cells in vitro, which express a set of specific markers and in vivo have the capacity to reconstruct the entire corneal epithelium in cases of ocular surface injury. Currently, LSC transplantation is a commonly used procedure in patients with ei...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.2009.00623.x
更新日期:2009-10-01 00:00:00
abstract:OBJECTIVES: The focus of this study was to determine the dedicator of cytokinesis 2 (DOCK2), extracellular signal-regulated kinase 1/2 (ERK1/2), c-Jun N-terminal kinase-1 (JNK) and Akt signals involved in CXCL13-mediated prostate cancer (PCa) cell invasion and proliferation. MATERIALS AND METHODS: Androgen-sensitive...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.2011.00757.x
更新日期:2011-08-01 00:00:00
abstract::In situ presence of numerous DNA strand breaks is a typical feature of apoptotic cells. Selective DNA strand break induction by photolysis (SBIP) at sites that contain incorporated halogenated DNA precursors has recently been proposed as a method of analysing DNA replication. Detection of DNA strand breaks, thus, enab...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.1995.tb00045.x
更新日期:1995-11-01 00:00:00
abstract:OBJECTIVES:Both interleukin-6 (IL-6) and transforming growth factor-beta (TGF-beta) are crucially involved in fibrotic events that characterize interstitial lung diseases (ILD). Therefore, the aim of this study was to investigate in primary cultures of normal and fibrotic human lung fibroblasts (HLF), exposed to either...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.2008.00538.x
更新日期:2008-06-01 00:00:00
abstract:OBJECTIVE:MicroRNAs (miRNAs) are negative regulators of gene expression that play important roles in cell processes such as proliferation, development and differentiation. Recently, it has been reported that miRNAs are related to development of carcinogenesis. The aim of this study was to identify miRNAs associated wit...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.2010.00717.x
更新日期:2011-02-01 00:00:00
abstract:OBJECTIVES:Oestrogen is known to inhibit osteoclastogenesis, and numerous studies have identified it as an autophagic activator. To date, the role of oestrogen in the autophagy of osteoclast precursors (OCPs) during osteoclastogenesis remains unclear. This study aimed to determine the effect of autophagy regulated by t...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12789
更新日期:2020-04-01 00:00:00
abstract:OBJECTIVES:The aim of the current study was to investigate whether nicotine treatment would induce the proliferation of isolated rat primary pancreatic acinar cells in culture by activating mitogen-activated protein kinase (MAPK) signalling and exocrine secretion. MATERIALS AND METHODS:A nicotine dose- and time-respon...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.2007.00418.x
更新日期:2007-02-01 00:00:00
abstract::On 31 May 2010, 14 072 567 bone marrow/apheresis donors registered in 44 countries and 426 501 cord blood units banked in 26 countries for public use were available to treat candidates to haemopoietic stem cell transplant lacking a family related compatible donor. Despite these impressive numbers, additional efforts a...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.2010.00720.x
更新日期:2011-04-01 00:00:00
abstract::Laminin-2 (LN-2, alpha2beta1gamma1) is a basement membrane-associated laminin isoform usually considered in the context of muscle and nerve tissues. To test the hypothesis that LN-2 can additionally modulate epithelial cell biology, an analysis of the role of LN-2 in cell adhesion, activation of signalling intermediat...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.2004.00292.x
更新日期:2004-04-01 00:00:00
abstract:OBJECTIVES:miR-21 can promote osteoblast differentiation of periodontal ligament stem cells. However, the effect of miR-21 on bone remodelling in the midpalatal suture is unclear. This study aimed to elucidate the effects of miR-21 on the midpalatal suture bone remodelling by expanding the palatal sutures. MATERIALS A...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12697
更新日期:2020-01-01 00:00:00
abstract:OBJECTIVES:Beryllium salts (here, beryllium sulphate) can produce a cytostatic effect in some cell types. The basis for this effect may include increased expression of proliferation inhibitors, reduced expression of proliferation promoters, or both. This study sought to determine the role of p53, the tumour-suppressing...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12291
更新日期:2016-12-01 00:00:00
abstract::WR-1065 (2-[(aminopropyl)amino]ethanethiol) reduces cytotoxic and mutagenic effects caused by exposure of cells to radiation and chemotherapeutic drugs, but the mechanisms involved are not fully known. We have observed an accumulation of cells in G2 in WR-1065 treated Chinese hamster ovary cells grown in alpha-minimal...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.1992.tb01466.x
更新日期:1992-11-01 00:00:00
abstract::Intra-erythrocytic Plasmodium species can be stained with the DNA binding dye, Hoechst 33342, and the distribution of DNA content determined for parasite populations by flow cytometric measurement of fluorescence. Analysis of this distribution will determine the parasitaemia (percentage of erythrocytes infected), and ...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.1992.tb01452.x
更新日期:1992-09-01 00:00:00
abstract::Studies on growth regulation in vitro to a large extent rely on comparison of growth curves. However, these do not discriminate between the relative contributions of the mitotic rate and the apoptotic rate to the net growth rate. In the present study, differential effects of 17beta-oestradiol (E2, 10(-8) M) and/or tam...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1046/j.1365-2184.1999.3250289.x
更新日期:1999-10-01 00:00:00
abstract:OBJECTIVE:Curcumin (capable of inhibiting angiogenic growth of human umbilical vein endothelial cells [HUVECs]), can be employed in vitro as a model of pathogenesis of corneal neovascularization (CRNV). The aim of this study was to explore regulatory mechanisms of microRNA (miR) levels after curcumin treatment. MATERI...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12289
更新日期:2016-12-01 00:00:00
abstract::A model of intestinal crypt organization is suggested based on the assumption that stem cells have a finite replicative life span. The model assumes the existence in a crypt of a quiescent ('deep') stem cell and a few more actively cycling ('proximate') stem cells. Monte Carlo computer simulation of published intestin...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/j.1365-2184.2006.00395.x
更新日期:2006-10-01 00:00:00
abstract:OBJECTIVES:Creation of functional, durable vasculature remains an important goal within the field of regenerative medicine. Engineered biological vasculature has the potential to restore or improve human tissue function. We hypothesized that the pleotropic effects of insulin-like growth factor 1 (IGF1) would enhance th...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12387
更新日期:2018-02-01 00:00:00
abstract:OBJECTIVES:Worldwide, lung cancer accounts for the majority of cancer-related deaths. Aberrant expression of miRNAs has increasingly been reported to be associated with tumour progression. This study aimed to explore the role of miR-301b in regulating apoptosis in lung cancer. MATERIALS AND METHODS:Expression of miR-3...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12264
更新日期:2016-08-01 00:00:00
abstract::Proliferation and multidrug resistance status are key predictors of therapeutic outcome in acute myeloid leukaemia (AML). Anthracyclines such as daunorubicin (DNR) are typically used to treat AML and can induce drug resistance. The goal of the studies described here was to select a combination of fluorescent probes th...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1046/j.1365-2184.1999.3240203.x
更新日期:1999-08-01 00:00:00
abstract::Reports on the p53-related cell cycle and apoptotic responses of EBV-transformed lymphoblastoid cell lines to DNA damage have led to some confusion. This may be due to differences in the nature of the specific p53 mutations under examination, but it can also be partly attributed to methodological and analytical proble...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:
更新日期:1999-02-01 00:00:00
abstract:OBJECTIVES:MicroRNAs are powerful regulators in hepatocellular carcinoma (HCC) tumorigenesis. MicoRNA-191 (miR-191) has been reported to play an important role in HCC, However, the regulatory mechanism is still unclear. In this study, we investigated the role of miR-191 in HCC and studied its underlying mechanisms of a...
journal_title:Cell proliferation
pub_type: 杂志文章
doi:10.1111/cpr.12635
更新日期:2019-09-01 00:00:00
abstract::Aberrant crypt foci (ACF) have been identified on the colonic mucosal surface of rodents treated with colon carcinogens and of humans after methylene-blue staining and observation under a light microscope. Several lines of evidence strongly suggest that ACF with certain morphological, histological, cell kinetics, and ...
journal_title:Cell proliferation
pub_type: 杂志文章,评审
doi:10.1046/j.1365-2184.2000.00159.x
更新日期:2000-02-01 00:00:00
abstract::Extracellular vesicles (EVs), a heterogeneous group of vesicles differing in size and shape, cargo content and function, are membrane-bound and nano-sized vesicles that could be released by nearly all variations of cells. EVs have gained considerable attention in the past decades for their functions in modulating inte...
journal_title:Cell proliferation
pub_type: 杂志文章,评审
doi:10.1111/cpr.12659
更新日期:2019-11-01 00:00:00