Abstract：
BACKGROUND/AIM:The outcome of patients with advanced hepatocellular carcinoma (HCC) remains poor and therapeutic options, including sorafenib, the first anti-cancer drug proved to prolong survival in patients with advanced HCC, are limited. However, no clinically useful predictive biomarker for sorafenib has been reported. MATERIALS AND METHODS:We exploited two-dimensional gel electrophoresis coupled with mass spectrometry to find de-regulated proteins by using conditioning of a sorafenib-resistant HCC cell line, Huh7. Tumor samples from 60 patients with HCC treated with sorafenib were analyzed and correlated with survival outcome. RESULTS:Comparative proteomics indicated three proteins including, 78 kDa glucose related protein (GRP78), 14-3-3ε, and heat shock protein 90β (HSP90β). The three proteins were over-expressed in sorafenib-resistant Huh7 cells. In HCC tumor samples from patients treated with sorafenib, 73% of tumor samples had a high expression of GRP78, 18% had high 14-3-3ε expression and 85% had high HSP90β expression. Among these, GRP78 was associated with the shortest progression-free survival of HCC patients treated with sorafenib. CONCLUSION:GRP78 can be a predictive biomarker in HCC patients treated with sorafenib. Strategies designed to inhibit the GRP78-related pathway may overcome sorafenib resistance.
journal_name
Cancer Genomics Proteomicsjournal_title
Cancer genomics & proteomicsauthors
Feng YH,Tung CL,Su YC,Tsao CJ,Wu TFdoi
10.21873/cgp.20159subject
Has Abstractpub_date
2019-11-01 00:00:00pages
569-576issue
6eissn
1109-6535issn
1790-6245pii
16/6/569journal_volume
16pub_type
杂志文章abstract：AIM:To identify and study targets of microRNA biomarkers of glioblastoma survival across events (death and recurrence) and phases (life expectancy or post-diagnostic) using functional and network analyses. MATERIALS AND METHODS:microRNAs associated with glioblastoma survival within and across race, gender, recurrence,...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章
doi：
更新日期：2011-07-01 00:00:00
abstract：BACKGROUND:Metastasis-associated protein 1 (MTA1) plays an important role in tumourigenesis and progression of certain cancer types. In the current study, we analyzed the relationship between MTA1 expression and disease progression of colorectal cancer (CRC). MATERIALS AND METHODS:CRC tissues (n=93) and adjacent norma...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章
doi：
更新日期：2015-11-01 00:00:00
abstract：:We recently reported that functional loss of p53 altered the responses of human HCT116 colon cancer cells to apoptosis triggers. To examine the molecular basis underlying the differential responses to drug treatment in the cancer cells, we performed a proteomic analysis in order to compare the protein expressions betw...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章
doi：
更新日期：2012-03-01 00:00:00
abstract：BACKGROUND:Identification and characterization of genes that are relevant to pancreatic cancer remains a priority for developing detection and diagnostic tests and identifying targets for treatment. MATERIALS AND METHODS:In order to discover relevant genes, we developed a microarray composed of 5763 pancreas and pancr...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章
doi：
更新日期：2004-09-01 00:00:00
abstract：:Despite the approval of several molecular therapies in the last years, breast cancer-associated death ranks as the second highest in women. This is due to metastatic disease, which represents a challenge for treatment. A better understanding of the molecular mechanisms of metastasis is, therefore, of paramount importa...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章,评审
doi：10.21873/cgp.20062
更新日期：2018-01-01 00:00:00
abstract：:The small heterodimer partner (SHP) is a key regulator of genes involved in cholesterol-bile acid homeostasis and functions as a specific transcription repressor. Differential protein expression in the liver of transgenic mice expressing the human SHP gene was compared with wild-type animals. Liver protein extracts we...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章
doi：
更新日期：2006-03-01 00:00:00
abstract：:We present possible applications of Atomic Force Microscopy (AFM) as a force spectroscopy tool in genomics and proteomics. AFM applications in these fields have opened new opportunities for studying the mechanical properties of biomolecules and their interactions in their native environment, as well as in determining ...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章,评审
doi：
更新日期：2004-01-01 00:00:00
abstract：BACKGROUND/AIM:Dedifferentiated liposarcoma (DDLPS) is recalcitrant type of sarcoma. DDLPS has a low survival rate with high recurrence and metastasis. In the present study, we evaluated the efficacy of several drugs against doxorubicin-resistant DDLPS in a patient-derived orthotopic xenograft (PDOX) model for precisio...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章
doi：10.21873/cgp.20194
更新日期：2020-07-01 00:00:00
abstract：BACKGROUND/AIM:Prostate cancer is the most common malignancy in US males. African American men have higher incidence and mortality rates than European Americans. Five single nucleotide polymorphisms are associated with PCa. We hypothesized haplotypes inferred from these SNPs are also associated with PCa. PATIENTS AND ...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章
doi：10.21873/cgp.20076
更新日期：2018-05-01 00:00:00
abstract：BACKGROUND:Clinical observations indicate that tumour cells can acquire tolerance when an anticancer drug is administered repeatedly. In the present study, the gene expression in cisplatin-resistant cells was analysed to identify early changes in gene expression in the course of cisplatin exposure. MATERIALS AND METHO...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章
doi：
更新日期：2006-11-01 00:00:00
abstract：:Functional genomics has been applied in the study of human malignancies since the inception of this field nearly a decade ago. Microarray analysis has been specifically used in an attempt to re-classify carcinomas at the molecular level, to aid in diagnosis/prognosis and to predict how various types of tumour respond ...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章
doi：
更新日期：2007-07-01 00:00:00
abstract：BACKGROUND/AIM:The aim of this study was to assess the incidence of MSI in a large series of human hepatocellular carcinomas (HCC) with various etiologies. MATERIALS AND METHODS:The MSI status was determined by polymerase chain reaction (PCR) using 5 mononucleotide and 13 CAn dinucleotide repeats. RESULTS:None of the...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章
doi：10.21873/cgp.20043
更新日期：2017-09-01 00:00:00
abstract：:The concept of individualized cancer chemotherapy emerged three decades ago from the observation that a small fraction of cells in primary tumors can form colonies in soft agar similar to stem cells of the hematopoietic system. In a series of retrospective and prospective clinical studies, clonogenic tumor growth and ...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章,评审
doi：
更新日期：2007-05-01 00:00:00
abstract：:Sepantronium bromide (YM-155) is believed to elicit apoptosis and mitotic arrest in tumor cells by reducing (BIRC5, survivin) mRNA. In this study, we monitored changes in survivin mRNA and protein after treating MDA-MB-231 cells with YM-155 concurrent with evaluation of whole transcriptomic (WT) mRNA and long intergen...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章
doi：10.21873/cgp.20083
更新日期：2018-07-01 00:00:00
abstract：BACKGROUND:Salivary gland cancer is a common type of head and neck cancer characterized by occasional deep invasion and lung metastasis. The precise role of sex steroid hormones in salivary gland cancer is unclear. To address this issue, we investigated whether the estrogen axis modulates salivary adenocarcinoma (SAC) ...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章
doi：
更新日期：2016-07-01 00:00:00
abstract：:Testicular germ cell tumors (GCT) are the most frequent malignancy in young adults and arise from intratubular germ cell neoplasia undetermined (IGCNU, also referred to as carcinoma in situ, CIS). To determine the transcriptional programs involved in the transition from normal germ cells to GCT, and to further elucida...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章
doi：
更新日期：2007-09-01 00:00:00
abstract：BACKGROUND/AIM:Mounting evidence has shown that miRNAs play a critical role in the regulation of hematopoiesis of cell proliferation and apoptosis as well as in tumorigenesis. The miR146a rs2910164 polymorphism, which is closely responsive for its expression, has been reported to associate with the risk of several soli...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章
doi：10.21873/cgp.20178
更新日期：2020-03-01 00:00:00
abstract：:Backgound/Aim: Malignant mesothelioma (MM) is an aggressive and fatal pleural cancer. The cell secretome offers information allowing insight into the pathogenesis of MM while offering the possibility to identify potential therapeutic targets and biomarkers. In the present study the secretome protein profile of MM cell...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章
doi：10.21873/cgp.20023
更新日期：2017-03-01 00:00:00
abstract：AIM:Alpha B-Crystallin (CRYAB) is purported to be a metastasis suppressor protein, and lack or lower CRYAB expression is a prognostic biomarker for several types of cancer, such as that of the prostate and head and neck. However, the association of genomic variation of CRYAB and breast cancer is not well studied. The a...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章
doi：
更新日期：2011-09-01 00:00:00
abstract：BACKGROUND:Metastasis associated gene-1 (MTA1), was initially discovered in aggressive human cancer cell lines and has been subsequently associated with the invasiveness and metastatic potential of cancer cells. MATERIALS AND METHODS:In the present study, we evaluated the expression levels of MTA1 in a cohort of human...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章
doi：
更新日期：2015-05-01 00:00:00
abstract：:The perception of long non-coding RNAs as chunk RNA and transcriptional noise has been steadily replaced by their role as validated targets for a diverse set of physiological processes in the past few years. However, for the vast majority of lncRNAs their precise mode of action and physiological function remain to be ...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章,评审
doi：10.21873/cgp.20027
更新日期：2017-05-01 00:00:00
abstract：:Although the statistical probability of therapeutic success is known for larger groups of cancer patients, the clinical response to chemotherapy of the individual patient remains uncertain. It would be of great value to know whether or not an individual tumor responds to the proposed therapy. The concept of sensitivit...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章,评审
doi：
更新日期：2004-03-01 00:00:00
abstract：BACKGROUND/AIM:Epithelioid osteoblastoma is a rare benign tumor of the bone. Its pathogenesis is unknown and little is known regarding its genetic features. MATERIALS AND METHODS:Cytogenetic, RNA sequencing, reverse transcription polymerase chain reaction (RT-PCR), genomic PCR, and Sanger sequencing analyses were perf...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章
doi：10.21873/cgp.20141
更新日期：2019-09-01 00:00:00
abstract：BACKGROUND:The progression of colorectal cancer (CRC) mainly stems from the occurrence of somatic mutation. However, there is little information that can be used to comprehensively analyse the importance of germline variants in CRC patients. PATIENTS AND METHODS:The candidate germline variants between tumor relapse an...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章
doi：10.21873/cgp.20189
更新日期：2020-05-01 00:00:00
abstract：BACKGROUND:Fusion genes driving tumourigenesis have drawn the attention of researchers and oncologists. Despite the importance of such molecular alterations, there are no comprehensive reproducible methods for detecting fusion genes. MATERIALS AND METHODS:Nineteen paediatric brain tumours of five types, namely pilocyt...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章
doi：10.21873/cgp.20093
更新日期：2018-07-01 00:00:00
abstract：:Breast cancer in men (MBC) is an uncommon malignancy and accounts for only 1% of all diagnosed breast cancers. By using genomic and transcriptomic approaches, researchers have been able to expand our insight into the genetic basis of breast cancer, by providing new biomarkers. We currently know that gene analysis by i...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章,评审
doi：
更新日期：2016-05-01 00:00:00
abstract：BACKGROUND/AIM:The altered cellular repair capacity plays a critical role in genomic instability and carcinogenesis. We aimed at evaluating the contribution of the polymorphic variant in apurinic/apyriminidinic endonuclease (APEX1) gene to its mRNA and protein levels and the risk of endometriosis. PATIENTS AND METHODS...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章
doi：
更新日期：2014-11-01 00:00:00
abstract：:The expression profiles of the erythropoietin producing hepatocellular carcinoma (Eph) receptor family of tyrosine kinases have been previously shown to provide molecular signatures of normal breast cells, breast tumor cells and invasive breast carcinoma cells. In particular, the expression of EphB6 receptor is lost i...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章
doi：
更新日期：2010-09-01 00:00:00
abstract：:Breast cancer metastasis accounts for the majority of deaths from breast cancer. Detection of breast cancer metastasis at the earliest stage is important for the management and prediction of breast cancer progression. Emerging techniques using the analysis of circulating tumor cells show promising results in predictin...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章,评审
doi：
更新日期：2012-09-01 00:00:00
abstract：:TACC2 is a member of the transforming acidic coiled-coil-containing protein family and is associated with the centrosome-spindle apparatus during cell cycling. The TACC2 gene is expressed in various splice forms predominantly in postmitotic tissues, including heart, muscle, kidney, and brain. Recent work has shown tha...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章
doi：
更新日期：2010-03-01 00:00:00
