Abstract:
:Testicular germ cell tumors (GCT) are the most frequent malignancy in young adults and arise from intratubular germ cell neoplasia undetermined (IGCNU, also referred to as carcinoma in situ, CIS). To determine the transcriptional programs involved in the transition from normal germ cells to GCT, and to further elucidate genetic differences between seminomas and non-seminomatous GCT the global expression profile of 12 neoplastic and 3 normal testicular tissues were investigated by whole genome cDNA microarrays. Transcriptional differences between seminomas and embryonal carcinomas were determined and gene signatures characterizing histological subtypes of GCT were identified. The most significant difference between seminomas and embryonal carcinomas was the expression of spermatogenesis-associated genes (PRAME, MAGEA4, SPAG1, HPX) in seminomas and regulatory genes DNMT3B and SOX2 as well as small molecular weight keratins KRT8, KRT18 in embryonal carcinomas. The expression of several selected genes (CK18, MAGE-A4, SOX2, DNMT3B, CD30, KIT) was studied by immunohistochemistry or reverse transcriptase-polymerase chain reaction (RT-PCR) in a large collective of GCT. In summary, our data identified tumor type-specific gene signatures of GCT and provided new insights into genetic pathways driving the transition to seminomas and embryonal carcinomas from their respective precursor lesions.
journal_name
Cancer Genomics Proteomicsjournal_title
Cancer genomics & proteomicsauthors
Biermann K,Heukamp LC,Steger K,Zhou H,Franke FE,Sonnack V,Brehm R,Berg J,Bastian PJ,Muller SC,Wang-Eckert L,Buettner Rsubject
Has Abstractpub_date
2007-09-01 00:00:00pages
359-67issue
5eissn
1109-6535issn
1790-6245journal_volume
4pub_type
杂志文章abstract::Tumorigenesis of glioblastoma multiforme (GBM), the most aggressive primary intracranial neoplasm, is associated with aberrant PI3K/AKT/mTOR signaling. Inhibitors of mTOR, such as rapamycin (RAPA) or its analogs, have provided limited benefit. Here, we aim to decipher the signaling pathways involved in RAPA resistance...
journal_title:Cancer genomics & proteomics
pub_type: 杂志文章
doi:
更新日期:2009-09-01 00:00:00
abstract:BACKGROUND:The progression of colorectal cancer (CRC) mainly stems from the occurrence of somatic mutation. However, there is little information that can be used to comprehensively analyse the importance of germline variants in CRC patients. PATIENTS AND METHODS:The candidate germline variants between tumor relapse an...
journal_title:Cancer genomics & proteomics
pub_type: 杂志文章
doi:10.21873/cgp.20189
更新日期:2020-05-01 00:00:00
abstract:BACKGROUND:Pancreatic cancer, has a very high mortality rate and requires novel molecular targets for diagnosis and therapy. Genetic association studies over databases offer an attractive starting point for gene discovery. MATERIALS AND METHODS:The National Center for Biotechnology Information (NCBI) Phenome Genome In...
journal_title:Cancer genomics & proteomics
pub_type: 杂志文章
doi:
更新日期:2015-01-01 00:00:00
abstract::Human telomerase reverse transcriptase (hTERT) is overexpressed in prostate cancer. Estrogen plays a central role in the development of prostate cancer. hTERT activity has been shown to be increased after estrogen treatment. Although significant efforts have been made to understand the role of estrogen, the telomerase...
journal_title:Cancer genomics & proteomics
pub_type: 杂志文章
doi:
更新日期:2009-11-01 00:00:00
abstract::Patients with pancreatic ductal adenocarcinoma have a dismall prognosis because at the time of diagnosis, in the vast majority of patients the tumor has already disseminated to distant organs and the therapeutic benefit of approved agents such as gemcitabine is limited. Therefore, the identification and preclinical an...
journal_title:Cancer genomics & proteomics
pub_type: 杂志文章,评审
doi:10.21873/cgp.20149
更新日期:2019-11-01 00:00:00
abstract:BACKGROUND:Oncogenic infection by HPV, eventually leads to cervical carcinogenesis, associated by deregulation of specific pathways and protein expression at the intracellular and secretome level. Thus, secretome analysis can elucidate the biological mechanisms contributing to cervical cancer. In the present study we s...
journal_title:Cancer genomics & proteomics
pub_type: 杂志文章,评审
doi:10.21873/cgp.20060
更新日期:2017-11-01 00:00:00
abstract:BACKGROUND/AIM:Medulloblastoma (MBL), an archetypal primitive neuroectodermal tumor of the cerebellum, is the most common pediatric central nervous system malignancy representing approximately 20% of all childhood brain tumors. Herein, we report on a new xenotransplantable tumor cell line, derived from a 6-year-old fem...
journal_title:Cancer genomics & proteomics
pub_type: 杂志文章
doi:10.21873/cgp.20037
更新日期:2017-07-01 00:00:00
abstract:BACKGROUND:Metastasis-associated protein 1 (MTA1) plays an important role in tumourigenesis and progression of certain cancer types. In the current study, we analyzed the relationship between MTA1 expression and disease progression of colorectal cancer (CRC). MATERIALS AND METHODS:CRC tissues (n=93) and adjacent norma...
journal_title:Cancer genomics & proteomics
pub_type: 杂志文章
doi:
更新日期:2015-11-01 00:00:00
abstract:BACKGROUND/AIM:The chromosome translocation t(14;21)(q11;q22) was reported in four pediatric T-cell lymphoblastic leukemias and was shown to activate the OLIG2 gene. MATERIALS AND METHODS:A pediatric T-cell lymphoblastic lymphoma was investigated using G-banding chromosome analysis, fluorescence in situ hybridization ...
journal_title:Cancer genomics & proteomics
pub_type: 杂志文章
doi:10.21873/cgp.20166
更新日期:2020-01-01 00:00:00
abstract::After the completion of sequencing for dozens of genomes, as well as the draft of human genome, a major challenge is to characterize genome-wide transcriptional regulation networks. Identification of regulatory functions for transcription factor binding sites in eukaryotes greatly enhances our understanding of the net...
journal_title:Cancer genomics & proteomics
pub_type: 杂志文章
doi:
更新日期:2008-01-01 00:00:00
abstract:BACKGROUND:Cancer is a major public health problem worldwide, and the number of incident cases increases every year expected to reach 17.1 million a year by 2020. There is evidence that people who adhere to the Mediterranean Diet (MediD) have lower incidence of cancer. However, cancers' location and culture studies see...
journal_title:Cancer genomics & proteomics
pub_type: 杂志文章,评审
doi:10.21873/cgp.20050
更新日期:2017-11-01 00:00:00
abstract:BACKGROUND/AIM:The altered cellular repair capacity plays a critical role in genomic instability and carcinogenesis. We aimed at evaluating the contribution of the polymorphic variant in apurinic/apyriminidinic endonuclease (APEX1) gene to its mRNA and protein levels and the risk of endometriosis. PATIENTS AND METHODS...
journal_title:Cancer genomics & proteomics
pub_type: 杂志文章
doi:
更新日期:2014-11-01 00:00:00
abstract:BACKGROUND:Inositol hexakisphosphate (IP6), a naturally occurring polyphosphorylated carbohydrate, has been reported to have significant in vivo and in vitro anticancer activity against numerous tumors. However, the molecular mechanism of the anticancer effect of IP6 has not been fully elucidated. MATERIALS AND METHOD...
journal_title:Cancer genomics & proteomics
pub_type: 杂志文章
doi:
更新日期:2007-01-01 00:00:00
abstract:BACKGROUND:The transcription factor SOX2 controls gene expression during development and has roles in both neurogenesis and gliogenesis. MATERIALS AND METHODS:The current study utilized immunohistochemistry, immunofluorescence, molecular genetics (gene copy number analysis) and Western blotting to examine SOX2 express...
journal_title:Cancer genomics & proteomics
pub_type: 杂志文章
doi:
更新日期:2011-05-01 00:00:00
abstract::The perception of long non-coding RNAs as chunk RNA and transcriptional noise has been steadily replaced by their role as validated targets for a diverse set of physiological processes in the past few years. However, for the vast majority of lncRNAs their precise mode of action and physiological function remain to be ...
journal_title:Cancer genomics & proteomics
pub_type: 杂志文章,评审
doi:10.21873/cgp.20027
更新日期:2017-05-01 00:00:00
abstract:BACKGROUND:Few studies have analyzed the association between human telomerase reverse transcriptase (hTERT) protein expression (nuclear and cytoplasmic localization), hTERT methylation status, and human papillomavirus (HPV) genotype infection in cervical cancer. PATIENTS AND METHODS:One hundred seventy-three patients ...
journal_title:Cancer genomics & proteomics
pub_type: 杂志文章
doi:10.21873/cgp.20218
更新日期:2020-09-01 00:00:00
abstract:BACKGROUND:Temozolomide-resistant (TMZ-R) glioblastoma is very difficult to treat, and a novel approach to overcome resistance is needed. MATERIALS AND METHODS:The efficacy of a combination treatment of STAT3 inhibitor, STX-0119, with rapamycin was investigated against our established TMZ-resistant U87 cell line. RES...
journal_title:Cancer genomics & proteomics
pub_type: 杂志文章
doi:10.21873/cgp.20021
更新日期:2017-01-02 00:00:00
abstract::We recently reported that functional loss of p53 altered the responses of human HCT116 colon cancer cells to apoptosis triggers. To examine the molecular basis underlying the differential responses to drug treatment in the cancer cells, we performed a proteomic analysis in order to compare the protein expressions betw...
journal_title:Cancer genomics & proteomics
pub_type: 杂志文章
doi:
更新日期:2012-03-01 00:00:00
abstract:BACKGROUND:Genetic differences between pulmonary typical carcinoids (TCs) and atypical carcinoids (ATCs) still remain controversial and no genetic marker is available for the evaluation of the malignant potential in pulmonary carcinoids. MATERIALS AND METHODS:Five TCs and 3 ATCs were analyzed concurrently by comparati...
journal_title:Cancer genomics & proteomics
pub_type: 杂志文章
doi:
更新日期:2006-01-01 00:00:00
abstract::Although several microscopic techniques are available for separation of small groups of cells from surrounding tissue, the high energy concentrated into a small area, the easy control of beam position and the lack of direct contact with the material to be dissected make lasers the best option for a large scale microdi...
journal_title:Cancer genomics & proteomics
pub_type: 杂志文章,评审
doi:
更新日期:2006-05-01 00:00:00
abstract:BACKGROUND:Cowden syndrome (CS) is a rare autosomal-dominant inherited disorder characterized by multiple hamartomas. While the hamartomas are benign, patients with CS have increased risk of osteosarcoma and of breast, thyroid, endometrial, soft-tissue and colonic neoplasms. Germline mutations of phosphatase and tensin...
journal_title:Cancer genomics & proteomics
pub_type: 杂志文章
doi:10.21873/cgp.20069
更新日期:2018-03-01 00:00:00
abstract:BACKGROUND/AIM:Pseudomyogenic hemangioendothelioma is a rare endothelial tumor. Previous genetic investigations have shown that the tumors carry either a SERPINE1-FOSB or an ACTB-FOSB fusion gene. The aim of the study was to identify FOSB fusions linked with pseudomyogenic hemangioendothelioma. MATERIALS AND METHODS:R...
journal_title:Cancer genomics & proteomics
pub_type: 杂志文章
doi:10.21873/cgp.20134
更新日期:2019-07-01 00:00:00
abstract:BACKGROUND:Cigarette smoke (CS) is the main inducer of oral cancer, increasing its prevalence by 4-7 times. MATERIALS AND METHODS:We examined the suitability of cell models SCC-25 and SCC-15 for studying effects of CS on oral cancer cells and whether CS significantly affects the cell cycle using fluorescence-activated...
journal_title:Cancer genomics & proteomics
pub_type: 杂志文章
doi:
更新日期:2015-03-01 00:00:00
abstract:BACKGROUND:Patients with metastazing malignant melanoma have a poor outcome and determination of thickness of the primary tumor remains as the most important prognostic predictor. The aim of this study was to use an antibody-based proteomics strategy to search for new molecular markers associated with melanoma progress...
journal_title:Cancer genomics & proteomics
pub_type: 杂志文章
doi:
更新日期:2008-11-01 00:00:00
abstract:BACKGROUND:Salivary gland cancer is a common type of head and neck cancer characterized by occasional deep invasion and lung metastasis. The precise role of sex steroid hormones in salivary gland cancer is unclear. To address this issue, we investigated whether the estrogen axis modulates salivary adenocarcinoma (SAC) ...
journal_title:Cancer genomics & proteomics
pub_type: 杂志文章
doi:
更新日期:2016-07-01 00:00:00
abstract:BACKGROUND/AIM:In a previous study we analyzed poliomyelitis incidence in US states, before the introduction of the polio vaccine. We noted an inverse correlation between polio incidence in US states, between 1937 and 1951, and colorectal cancer incidence (2005-2009). To further elucidate the role that poliovirus could...
journal_title:Cancer genomics & proteomics
pub_type: 杂志文章
doi:10.21873/cgp.20112
更新日期:2019-01-01 00:00:00
abstract::TACC2 is a member of the transforming acidic coiled-coil-containing protein family and is associated with the centrosome-spindle apparatus during cell cycling. The TACC2 gene is expressed in various splice forms predominantly in postmitotic tissues, including heart, muscle, kidney, and brain. Recent work has shown tha...
journal_title:Cancer genomics & proteomics
pub_type: 杂志文章
doi:
更新日期:2010-03-01 00:00:00
abstract::The concept of individualized cancer chemotherapy emerged three decades ago from the observation that a small fraction of cells in primary tumors can form colonies in soft agar similar to stem cells of the hematopoietic system. In a series of retrospective and prospective clinical studies, clonogenic tumor growth and ...
journal_title:Cancer genomics & proteomics
pub_type: 杂志文章,评审
doi:
更新日期:2007-05-01 00:00:00
abstract::Epidemiological studies have established a causal relationship between cigarette smoking and respiratory tract cancer. The knowledge about the interaction of tobacco constituents with cellular systems is, however, still incomplete. Therefore, we analyzed 36 factors with known or assumed relevance and found that 8 prot...
journal_title:Cancer genomics & proteomics
pub_type: 杂志文章
doi:
更新日期:2004-05-01 00:00:00
abstract::Cellular signal transduction pathways and gene expression are tightly regulated to accommodate changes in response to physiological environments. In the current study, molecules were identified that are activated as a result of intracellular signaling and immediately expressed as mRNA in MCF-7 breast cancer cells shor...
journal_title:Cancer genomics & proteomics
pub_type: 杂志文章
doi:
更新日期:2008-05-01 00:00:00