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Low incidence of DNA sequence variation in human induced pluripotent stem cells generated by nonintegrating plasmid expression.

Abstract：

:The utility of induced pluripotent stem cells (iPSCs) as models to study diseases and as sources for cell therapy depends on the integrity of their genomes. Despite recent publications of DNA sequence variations in the iPSCs, the true scope of such changes for the entire genome is not clear. Here we report the whole-genome sequencing of three human iPSC lines derived from two cell types of an adult donor by episomal vectors. The vector sequence was undetectable in the deeply sequenced iPSC lines. We identified 1,058-1,808 heterozygous single-nucleotide variants (SNVs), but no copy-number variants, in each iPSC line. Six to twelve of these SNVs were within coding regions in each iPSC line, but ~50% of them are synonymous changes and the remaining are not selectively enriched for known genes associated with cancers. Our data thus suggest that episome-mediated reprogramming is not inherently mutagenic during integration-free iPSC induction.

journal_name

Cell Stem Cell

journal_title

Cell stem cell

authors

Cheng L,Hansen NF,Zhao L,Du Y,Zou C,Donovan FX,Chou BK,Zhou G,Li S,Dowey SN,Ye Z,NISC Comparative Sequencing Program.,Chandrasekharappa SC,Yang H,Mullikin JC,Liu PP

doi

10.1016/j.stem.2012.01.005

subject

Has Abstract

pub_date

2012-03-02 00:00:00

pages

337-44

issue

3

eissn

1934-5909

issn

1875-9777

pii

S1934-5909(12)00007-0

journal_volume

10

pub_type

杂志文章

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