Abstract：
:Pluripotent stem cells are defined by their capacity to differentiate into all three tissue layers that comprise the body. Chimera formation, generated by stem cell transplantation to the embryo, is a stringent assessment of stem cell pluripotency. However, the ability of human pluripotent stem cells (hPSCs) to form embryonic chimeras remains in question. Here we show using a stage-matching approach that human induced pluripotent stem cells (hiPSCs) and human embryonic stem cells (hESCs) have the capacity to participate in normal mouse development when transplanted into gastrula-stage embryos, providing in vivo functional validation of hPSC pluripotency. hiPSCs and hESCs form interspecies chimeras with high efficiency, colonize the embryo in a manner predicted from classical developmental fate mapping, and differentiate into each of the three primary tissue layers. This faithful recapitulation of tissue-specific fate post-transplantation underscores the functional potential of hPSCs and provides evidence that human-mouse interspecies developmental competency can occur.
journal_name
Cell Stem Celljournal_title
Cell stem cellauthors
Mascetti VL,Pedersen RAdoi
10.1016/j.stem.2015.11.017subject
Has Abstractpub_date
2016-01-07 00:00:00pages
67-72issue
1eissn
1934-5909issn
1875-9777pii
S1934-5909(15)00515-9journal_volume
18pub_type
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