Abstract：
:In-depth analysis of the cellular and molecular mechanisms regulating human HSC function will require a surrogate host that supports robust maintenance of transplanted human HSCs in vivo, but the currently available options are problematic. Previously we showed that mutations in the Kit receptor enhance engraftment of transplanted HSCs in the mouse. To generate an improved model for human HSC transplantation and analysis, we developed immune-deficient mouse strains containing Kit mutations. We found that mutation of the Kit receptor enables robust, uniform, sustained, and serially transplantable engraftment of human HSCs in adult mice without a requirement for irradiation conditioning. Using this model, we also showed that differential KIT expression identifies two functionally distinct subpopulations of human HSCs. Thus, we have found that the capacity of this Kit mutation to open up stem cell niches across species barriers has significant potential and broad applicability in human HSC research.
journal_name
Cell Stem Celljournal_title
Cell stem cellauthors
Cosgun KN,Rahmig S,Mende N,Reinke S,Hauber I,Schäfer C,Petzold A,Weisbach H,Heidkamp G,Purbojo A,Cesnjevar R,Platz A,Bornhäuser M,Schmitz M,Dudziak D,Hauber J,Kirberg J,Waskow Cdoi
10.1016/j.stem.2014.06.001subject
Has Abstractpub_date
2014-08-07 00:00:00pages
227-38issue
2eissn
1934-5909issn
1875-9777pii
S1934-5909(14)00249-5journal_volume
15pub_type
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