当前位置： SCI文献检索 > JOURNAL OF MICROBIOLOGICAL METHODS期刊下所有文献 > YTOX: a rapid toxicity test based on the dehydrogenase activity of Saccharomyces cerevisiae for detection of contaminants in water samples.

YTOX: a rapid toxicity test based on the dehydrogenase activity of Saccharomyces cerevisiae for detection of contaminants in water samples.

Abstract：

:A simple generic toxicity method (test) is proposed using baker's yeast to mediate the reduction of the colourless triphenyltetrazolium chloride (TTC) to red, 1,3,5-triphenyl formazan, which can be extracted by dimethyl sulfoxide (DMSO), enabling the identification of reducible toxic compounds (e.g. cadmium, fipronil) in water for consumption.

journal_name

J Microbiol Methods

journal_title

Journal of microbiological methods

authors

Gomes LH,Duarte KMR,Kamogawa MY,Ferrarezi JA,Andrino FG,Tavares ACL,da Silva APM,Laguna SE,de Lira SP

doi

10.1016/j.mimet.2019.04.008

subject

Has Abstract

pub_date

2019-06-01 00:00:00

pages

43-46

eissn

0167-7012

issn

1872-8359

pii

S0167-7012(19)30108-3

journal_volume

161

pub_type

杂志文章

相关文献

JOURNAL OF MICROBIOLOGICAL METHODS文献大全
  • Exopolysaccharide assay in Escherichia coli microcolonies using a cleavable fusion protein of GFP-labeled carbohydrate-binding module.

    abstract：:A fused protein composed of a carbohydrate-binding module and green fluorescence protein (GFP) was developed to measure the exopolysaccharides (EPShs) present in Escherichia coli microcolonies. The cleavage of the GFP part of this protein using a site-specific protease allowed for the non-invasive and quantitative eva...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2015.05.013

    authors： Ojima Y,Suparman A,Nguyen MH,Sakka M,Sakka K,Taya M

    更新日期：2015-07-01 00:00:00

  • Bacterial identification using a SCIEX 5800 TOF/TOF MALDI research instrument and an external database.

    abstract：:In our current study we were identifying 26 bacterial isolates using a SCIEX 5800 TOF/TOF MALDI instrument and an external database. The results were compared with the results of a Vitek® MS system and in case of discrepancies at the species level 16s rRNA sequencing was performed for further verification. ...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2019.105685

    authors： Huber CA,Pflüger V,Reed S,Cottrell K,Sidjabat HE,Ranasinghe A,Zowawi HM,Harris P,Paterson DL

    更新日期：2019-09-01 00:00:00

  • Rapid antibiotic susceptibility phenotypic characterization of Staphylococcus aureus using automated microscopy of small numbers of cells.

    abstract：:Staphylococcus aureus remains a leading, virulent pathogen capable of expressing complex drug resistance that requires up to 2-4 days for laboratory analysis. In this study, we evaluate the ability of automated microscopy of immobilized live bacterial cells to differentiate susceptible from non-susceptible responses o...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2013.12.021

    authors： Price CS,Kon SE,Metzger S

    更新日期：2014-03-01 00:00:00

  • Improved detection of microbial DNA after bead-beating before DNA isolation.

    abstract：:Detection of microbial DNA in clinical samples by real-time PCR requires a universal extraction method with equally efficient lysis of cell walls of all possible microorganisms. We demonstrated that physical disruption by bead-beating with 0.1mm beads in combination with MagNA Pure DNA III extraction enhances microbia...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2009.11.009

    authors： de Boer R,Peters R,Gierveld S,Schuurman T,Kooistra-Smid M,Savelkoul P

    更新日期：2010-02-01 00:00:00

  • Stable isotope probing: technical considerations when resolving (15)N-labeled RNA in gradients.

    abstract：:RNA based stable isotope probing (SIP) facilitates the detection and identification of active members of microbial populations that are involved in the assimilation of an isotopically labeled compound. (15)N-RNA-SIP is a new method that has been discussed in recent literature but has not yet been tested. Herein, we de...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2009.11.002

    authors： Addison SL,McDonald IR,Lloyd-Jones G

    更新日期：2010-01-01 00:00:00

  • Quantitative characterization of different strains of Saccharomyces yeast by analysis of fluorescence microscopy images of cell populations.

    abstract：:Much of our knowledge concerned with microbial cells is based on population-based analysis of cultures, which give useful insights into average responses but neither on individual cells nor subpopulations. In this work we demonstrate how to access and utilise large amounts of valuable information concerned with cell p...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2009.01.011

    authors： Bhatta H,Goldys EM

    更新日期：2009-04-01 00:00:00

  • Advancement of a multiplex PCR for the differentiation of all currently described Brucella species.

    abstract：:To facilitate routine laboratories in the effective diagnosis of brucellosis, we report a robust and rapid multiplex PCR assay, which allows for the differentiation of all nine currently recognised Brucella species. This includes the recently described species B. microti, B. inopinata, B. ceti and B. pinnipedialis. ...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2009.10.015

    authors： Mayer-Scholl A,Draeger A,Göllner C,Scholz HC,Nöckler K

    更新日期：2010-01-01 00:00:00

  • An efficient and simple method to increase the level of displayed protein on the yeast cell surface.

    abstract：BACKGROUND:The development of oral vaccines using yeast surface display technology is an area of intensive study in vaccine development, but the protein level displayed on yeast surfaces is not currently high enough to obtain a robust immune response. METHODS:To address this issue, we established an efficient and simp...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2017.02.002

    authors： Zhao JZ,Xu LM,Liu M,Cao YS,LaPatra SE,Yin JS,Liu HB,Lu TY

    更新日期：2017-04-01 00:00:00

  • Comparison of freeze-thaw cycles for nucleic acid extraction and molecular detection of Cryptosporidium parvum and Toxoplasma gondii oocysts in environmental matrices.

    abstract：:Freeze-thaw DNA extraction methods and PCR primers were compared to optimize detection of Cryptosporidium parvum and Toxoplasma gondii oocysts in different matrices. Increasing FT cycles did not increase parasite DNA detection, and primers targeting the 18S ssrRNA gene yielded the most sensitive detection of C. parvum...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2018.11.017

    authors： Manore AJW,Harper SL,Aguilar B,Weese JS,Shapiro K

    更新日期：2019-01-01 00:00:00

  • High resolution clear native electrophoresis is a good alternative to blue native electrophoresis for the characterization of the Escherichia coli membrane complexes.

    abstract：:Blue native electrophoresis (BNE) has become the most popular method for the global analysis of membrane protein complexes. Although it has been shown to be very useful for that purpose, it can produce the dissociation of complexes with weak interactions and, due to the use of Coomassie Brilliant Blue, does not allow ...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2014.05.003

    authors： Diéguez-Casal E,Freixeiro P,Costoya L,Criado MT,Ferreirós C,Sánchez S

    更新日期：2014-07-01 00:00:00

  • Strain development and optimized fermentation conditions for blood meal using Aspergillus niger and Aspergillus oryzae.

    abstract：:To hydrolyze blood meal (BM) effectively, two mutants were generated using ultra-violet mutagenesis. Single-factor experiments, the Plackett-Burman technique and response surface methodology were used to optimize the fermentation conditions. This study successfully generated a mutant and innovatively provided importan...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2014.03.017

    authors： Zheng Y,Zhang H,Wang D,Gao P,Shan A

    更新日期：2014-06-01 00:00:00

  • Differentiation of Salmonella enterica serotype gallinarum biotype pullorum from biotype gallinarum by analysis of phase 1 flagellin C gene (fliC).

    abstract：:Salmonella enterica serotype gallinarum biotype gallinarum and biotype pullorum are non-motile and pathogenic avian strains. Biotype gallinarum causes fowl typhoid and biotype pullorum is the cause of pullorum disease in chickens. The two biotypes could be differentiated based on biochemical characteristics. However, ...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/s0167-7012(99)00129-3

    authors： Kwon HJ,Park KY,Yoo HS,Park JY,Park YH,Kim SJ

    更新日期：2000-03-01 00:00:00

  • Development and validation of evolutionary algorithm software as an optimization tool for biological and environmental applications.

    abstract：:A flexible, extendable tool for the optimization of (micro)biological processes and protocols using evolutionary algorithms was developed. It has been tested using three different theoretical optimization problems: 2 two-dimensional problems, one with three maxima and one with five maxima and a river autopurification ...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2004.01.004

    authors： Sys K,Boon N,Verstraete W

    更新日期：2004-06-01 00:00:00

  • A new method for the preservation of axenic fungal cultures.

    abstract：:Microbiological work requires a reliable source of cultures that are not only well defined and taxonomically determined, but are also adequately preserved without changes in their morphological, physiological and genetic traits. Here we describe an easy, cost effective and rapid method for reliably preserving filament...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2014.02.009

    authors： Hu X,Webster G,Xie L,Yu C,Li Y,Liao X

    更新日期：2014-04-01 00:00:00

  • Comparative evaluation of two matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) systems, Vitek MS and Microflex LT, for the identification of Gram-positive cocci routinely isolated in clinical microbiology laborat

    abstract：:We evaluated the performance of two MALDI-TOF MS systems for the identification of clinically important Gram-positive cocci. Vitek MS and Microflex LT correctly identified 97.2% and 94.7%, respectively. Both systems offer reliable and rapid identification of clinically important Gram-positive cocci isolated in clinica...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2015.03.020

    authors： Lee M,Chung HS,Moon HW,Lee SH,Lee K

    更新日期：2015-06-01 00:00:00

  • Molecular detection and quantification of viable probiotic strains in animal feedstuffs using the commercial direct fed microbial Lactobacillus animalis NP51 as a model.

    abstract：:Lactobacillus animalis NP51 is a direct-fed microbial strain (DFM) extensively used as a pre-harvest food safety mitigation in feedlot cattle due to its antagonistic effects against human foodborne pathogens such as Salmonella and Escherichia coli O157:H7. NP51 not only promotes overall gut health but interferes with ...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2018.04.012

    authors： Ayala DI,Chen JC,Bugarel M,Loneragan GH,den Bakker HC,Kottapalli KR,Brashears MM,Nightingale KK

    更新日期：2018-06-01 00:00:00

  • Building a molecular Listeria monocytogenes database to centralize and share PFGE typing data from food, environmental and animal strains throughout Europe.

    abstract：:The European Union Reference Laboratory (EURL) for Listeria monocytogenes (Lm) collaborates with a network of 35 National Reference Laboratories (NRLs) throughout Europe. Most of these NRLs are in charge of detecting and typing Lm strains from food, environment and animals, which are isolated nationally. The past few ...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2014.06.001

    authors： Félix B,Danan C,Van Walle I,Lailler R,Texier T,Lombard B,Brisabois A,Roussel S

    更新日期：2014-09-01 00:00:00

  • Assessment of bacterial community structure in soil by polymerase chain reaction and denaturing gradient gel electrophoresis.

    abstract：:Bacterial community structure was studied in a Flevo silt loam (FSL) soil microplot, as well as in 15 other soils, by using DNA extraction followed by molecular fingerprinting. Total community DNA was extracted and purified by a direct method, which yielded amplifiable DNA of high molecular weight for all soils. A var...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/s0167-7012(99)00054-8

    authors： Gelsomino A,Keijzer-Wolters AC,Cacco G,van Elsas JD

    更新日期：1999-10-01 00:00:00

  • A new chromogenic agar medium for detection of potentially virulent Yersinia enterocolitica.

    abstract：:Several outbreaks of foodborne yersiniosis have been documented and this disease continues to be source of infections transmitted through foods. The selective agars most commonly used to isolate Yersinia enterocolitica in clinical, food and environmental samples, cefsulodin-irgasan-novobiocin (CIN) and MacConkey (MAC)...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2007.11.019

    authors： Weagant SD

    更新日期：2008-02-01 00:00:00

  • Development of a surface plasmon resonance biosensor for the identification of Campylobacter jejuni.

    abstract：:The purpose of this study was to develop a biosensor based on surface plasmon resonance (SPR) for the rapid identification of C. jejuni in broiler samples. We examined the specificity and sensitivity of commercial antibodies against C. jejuni with six Campylobacter strains and six non-Campylobacter bacterial strains. ...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2006.12.002

    authors： Wei D,Oyarzabal OA,Huang TS,Balasubramanian S,Sista S,Simonian AL

    更新日期：2007-04-01 00:00:00

  • A new semi-nested PCR protocol to amplify large 18S rRNA gene fragments for PCR-DGGE analysis of soil fungal communities.

    abstract：:The analysis of soil fungal communities by molecular fingerprinting and subsequent identification of the underlying populations require the amplification of a phylogenetically informative gene fragment. In this study we tested the reliability and suitability of the previously published fungal primer combination (NS1/F...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2005.06.014

    authors： Oros-Sichler M,Gomes NC,Neuber G,Smalla K

    更新日期：2006-04-01 00:00:00

  • A chemical proteomics approach to identify c-di-GMP binding proteins in Pseudomonas aeruginosa.

    abstract：:In many bacteria, high levels of the ubiquitous second messenger c-di-GMP have been demonstrated to suppress motility and to promote the establishment of surface-adherent biofilm communities. While molecular mechanisms underlying the synthesis and degradation of c-di-GMP have been comprehensively characterized, little...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2011.11.015

    authors： Düvel J,Bertinetti D,Möller S,Schwede F,Morr M,Wissing J,Radamm L,Zimmermann B,Genieser HG,Jänsch L,Herberg FW,Häussler S

    更新日期：2012-02-01 00:00:00

  • A novel 96-well gel-based assay for determining antifungal activity against filamentous fungi.

    abstract：:In recent years the global rise in antibiotic resistance and environmental consciousness lead to a renewed fervour to find and develop novel antibiotics, including antifungals. However, the influence of the environment on antifungal activity is often disregarded and many in vitro assays may cause the activity of certa...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2012.09.025

    authors： Troskie AM,Vlok NM,Rautenbach M

    更新日期：2012-12-01 00:00:00

  • The first performance report for the Bio-Rad Dx CT/NG/MG assay for simultaneous detection of Chlamydia trachomatis, Neisseria gonorrhoeae and Mycoplasma genitalium in urogenital samples.

    abstract：:We evaluated the clinical performance of the Bio-Rad Dx CT/NG/MG assay for the detection of Chlamydia trachomatis, Mycoplasma genitalium and Neisseria gonorrhoeae in urogenital samples in comparison with the Roche COBAS® TaqMan® CT assay for C. trachomatis and an in-house TaqMan PCR assay for M. genitalium. Swab speci...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2012.03.009

    authors： Le Roy C,Le Hen I,Clerc M,Arfel V,Normandin F,Bébéar C,de Barbeyrac B

    更新日期：2012-06-01 00:00:00

  • Digital recordings of gas-vesicle collapse used to measure turgor pressure and cell-water relations of cyanobacterial cells.

    abstract：:The gas vesicles of the cyanobacterium Microcystis sp. collapse under pressures ranging from 0.65-1.10 MPa, determined from turbidity changes in a pressure nephelometer. In turgid cells, collapse occurs at a lower range of pressures; the difference is equal to the cell turgor pressure. The turgor pressure decreases, h...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2009.02.005

    authors： Holland DP,Walsby AE

    更新日期：2009-05-01 00:00:00

  • A novel selective medium for the isolation and distribution of Rothia dentocariosa in oral cavities.

    abstract：:Rothia dentocariosa is an indigenous microbial flora of dental plaque. To examine the bacterium population in oral cavities, a novel selective medium, designated RDSM, was developed for the isolation of R. dentocariosa. RDSM was prepared by adding 0.5 μg/ml of lincomycin, 10 μg/ml of colistin, and 40% sucrose to Heart...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2012.07.004

    authors： Uchibori S,Tsudukibashi O,Goto H,Kobayashi T,Aida M

    更新日期：2012-10-01 00:00:00

  • Antigenicity and immunogenicity of fused B-subunit of heat labile toxin of Escherichia coli and colonization factor antigen I polyepitopes.

    abstract：:Linear B-cell epitopes ((93)AKEFEAAAL(101) and (66)PQLTDVLN(73)) of CfaB were genetically fused to ltb-(gly)5-cfaB(1-25). Sera of rabbits immunized with fusion proteins reacted strongly with solid-phase bound ETEC bacteria bearing CFA/I fimbriae. Sera failed to agglutinate or inhibit hemagglutination promoted by CFA/I...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2014.07.035

    authors： Savar NS,Dashti A,Darzi Eslam E,Jahanian-Najafabadi A,Jafari A

    更新日期：2014-11-01 00:00:00

  • Agrobacterium-mediated transformation of the white-rot fungus Physisporinus vitreus.

    abstract：:The biotechnologically important white-rot fungus Physisporinus vitreus was co-cultivated with Agrobacterium tumefaciens AGL-1 carrying plasmids with nourseothricin resistance as the selectable marker gene and red fluorescence protein as a visual marker. Mitotically stable transformed isolates were obtained showing re...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2013.09.001

    authors： Schubert M,Stührk C,Fuhr MJ,Schwarze FW

    更新日期：2013-11-01 00:00:00

  • A panel of bioluminescent whole-cell bacterial biosensors for the screening for new antibacterial substances from natural extracts.

    abstract：:Whole-cell bacterial biosensors can be applied for the screening of antibacterial properties of extracts. We constructed a biosensor panel consisting of four different bacterial biosensor strains: Escherichia, Staphylococcus, Acinetobacter and Pseudomonas for expanded screening potential. The functionality of the pane...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2020.106083

    authors： Poikulainen E,Tienaho J,Sarjala T,Santala V

    更新日期：2020-10-14 00:00:00

  • MALDI-TOF MS based identification of food-borne yeast isolates.

    abstract：:In this study, food-borne yeast isolates (n=96), comprising at least 33 species, were identified using MALDI-TOF MS and conventional methods (API ID 32 C and Phoenix Yeast ID). Discrepancies of both methods were resolved by sequencing the ITS1-5.8S-rRNA-ITS2 region. For ten isolates, mainly classified to Rhodotorula a...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2014.08.021

    authors： Pavlovic M,Mewes A,Maggipinto M,Schmidt W,Messelhäußer U,Balsliemke J,Hörmansdorfer S,Busch U,Huber I

    更新日期：2014-11-01 00:00:00