Abstract：
:Salmonella enterica serotype gallinarum biotype gallinarum and biotype pullorum are non-motile and pathogenic avian strains. Biotype gallinarum causes fowl typhoid and biotype pullorum is the cause of pullorum disease in chickens. The two biotypes could be differentiated based on biochemical characteristics. However, conventional culture and biochemical assays are time-consuming, laborious and need sterile laboratory practices. Although the two biotypes, gallinarum and pullorum are non-motile, they possess the phase 1 flagellin C gene. The variable regions of the flagellin C gene from 41 biotype pullorum and 52 biotype gallinarum were amplified by colony-PCR and analyzed by single strand conformational polymorphism (SSCP) method. Differences in SSCP electrophoretic patterns were confirmed by nucleotide sequencing. In addition, PCR-RFLP with Hinp1I was also successfully applied to differentiate the two biotypes. These results suggested that the variable regions of fliC could be used as a genetic marker to differentiate biotype gallinarum from biotype pullorum.
journal_name
J Microbiol Methodsjournal_title
Journal of microbiological methodsauthors
Kwon HJ,Park KY,Yoo HS,Park JY,Park YH,Kim SJdoi
10.1016/s0167-7012(99)00129-3subject
Has Abstractpub_date
2000-03-01 00:00:00pages
33-8issue
1eissn
0167-7012issn
1872-8359pii
S0167-7012(99)00129-3journal_volume
40pub_type
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