Abstract:
:We have developed a novel flow cytometric procedure that allows determinations of properties of protein excretion in the growth medium on a cell-by-cell basis in Saccharomyces cerevisiae. The procedure is based on labelling of a periplasmically secreted protein with antibodies conjugated to a fluorescent marker such as fluorescein isothiocyanate (FITC). The staining conditions did not perturb cell growth after resuspension of stained cells in growth medium. Decrease in fluorescence was found to correlate with excretion of glucoamylase into the growth medium. The analysis of the staining pattern over time provides information on the behaviour of individual cells belonging to different cell-cycle phases and can be used to calculate the specific excretion rate of the overall population.
journal_name
J Microbiol Methodsjournal_title
Journal of microbiological methodsauthors
Porro D,Venturini M,Brambilla L,Alberghina L,Vanoni Mdoi
10.1016/s0167-7012(00)00171-8subject
Has Abstractpub_date
2000-09-01 00:00:00pages
49-55issue
1eissn
0167-7012issn
1872-8359pii
S0167-7012(00)00171-8journal_volume
42pub_type
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