Relating growth dynamics and glucoamylase excretion of individual Saccharomyces cerevisiae cells.

Abstract:

:We have developed a novel flow cytometric procedure that allows determinations of properties of protein excretion in the growth medium on a cell-by-cell basis in Saccharomyces cerevisiae. The procedure is based on labelling of a periplasmically secreted protein with antibodies conjugated to a fluorescent marker such as fluorescein isothiocyanate (FITC). The staining conditions did not perturb cell growth after resuspension of stained cells in growth medium. Decrease in fluorescence was found to correlate with excretion of glucoamylase into the growth medium. The analysis of the staining pattern over time provides information on the behaviour of individual cells belonging to different cell-cycle phases and can be used to calculate the specific excretion rate of the overall population.

journal_name

J Microbiol Methods

authors

Porro D,Venturini M,Brambilla L,Alberghina L,Vanoni M

doi

10.1016/s0167-7012(00)00171-8

subject

Has Abstract

pub_date

2000-09-01 00:00:00

pages

49-55

issue

1

eissn

0167-7012

issn

1872-8359

pii

S0167-7012(00)00171-8

journal_volume

42

pub_type

杂志文章
  • Concentration of viruses from environmental waters using nanoalumina fiber filters.

    abstract::Human viral contamination in drinking and recreational water may persist for extensive periods of time and cause a significant health risk concern. The aim of this study is to evaluate a viral recovery method using a new electropositive charged nanoalumina filter and to compare results with the widely used negatively ...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章

    doi:10.1016/j.mimet.2010.01.018

    authors: Li D,Shi HC,Jiang SC

    更新日期:2010-04-01 00:00:00

  • A PCR-based method for detection of potato pathogen, Helminthosporium solani, in silver scurf infected tuber tissue and soils.

    abstract::Silver scurf caused by Helminthosporium solani causes significant economic losses in table stock, seed and processing potatoes. Specific polymerase chain reaction (PCR) primers, Hs1F1/Hs2R1, from H. solani were used for the amplification of a 447-bp product from 20 tissue samples and 54 single spore H. solani isolates...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章

    doi:10.1016/s0167-7012(00)00240-2

    authors: Errampalli D,Saunders J,Cullen D

    更新日期:2001-02-01 00:00:00

  • Quantifying fungal infection of plant leaves by digital image analysis using Scion Image software.

    abstract::A digital image analysis method previously used to evaluate leaf color changes due to nutritional changes was modified to measure the severity of several foliar fungal diseases. Images captured with a flatbed scanner or digital camera were analyzed with a freely available software package, Scion Image, to measure chan...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章

    doi:10.1016/j.mimet.2008.03.008

    authors: Wijekoon CP,Goodwin PH,Hsiang T

    更新日期:2008-08-01 00:00:00

  • A novel high-resolution melting analysis-based method for Salmonella genotyping.

    abstract::To establish a simple and rapid high-resolution melting curve (HRM) method, 5 different strains of Salmonella were identified by adding DNA denaturants at different concentrations into the HRM system to change the characteristics of DNA melting and to obtain different Tm (dissolving temperature) values of DNA from dif...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章

    doi:10.1016/j.mimet.2019.105806

    authors: Hu M,Yang D,Wu X,Luo M,Xu F

    更新日期:2020-05-01 00:00:00

  • Suppression subtractive hybridisation and real-time PCR for strain-specific quantification of the probiotic Bifidobacterium animalis BAN in broiler feed.

    abstract::To ensure quality management during the production processes of probiotics and for efficacy testing in vivo, accurate tools are needed for the identification and quantification of probiotic strains. In this study, a strain-specific qPCR assay based on Suppression Subtractive Hybridisation (SSH) for identifying unique ...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章

    doi:10.1016/j.mimet.2016.02.011

    authors: Fibi S,Klose V,Mohnl M,Weber B,Haslberger AG,Sattler VA

    更新日期:2016-04-01 00:00:00

  • Rapid detection of Haemophilus parasuis using cross-priming amplification and vertical flow visualization.

    abstract::Haemophilus parasuis infection is of considerable economic importance in the swine industry due to high morbidity and mortality in naive swine populations. Accurate detection and identification of the causative agent are difficult, yet necessary, for disease control. In this study, a simple and rapid method of cross-p...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章

    doi:10.1016/j.mimet.2017.11.005

    authors: Gou H,Li J,Cai R,Song S,Li M,Yang D,Jiang Z,Li Y,Chu P,Li C

    更新日期:2018-01-01 00:00:00

  • Quantitative cell wall protein profiling of invasive and non-invasive Saccharomyces cerevisiae strains.

    abstract::A new, simple approach for the isolation and quantitative analysis of the cell wall (CW) proteins from invasively growing Saccharomyces cerevisiae strains is described in this contribution. The proposed method was proved compatible with agar-invasion assays and was demonstrated to be useful as a screening tool for rap...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章

    doi:10.1016/j.mimet.2009.09.003

    authors: Zupan J,Mavri J,Raspor P

    更新日期:2009-12-01 00:00:00

  • Assessment of bacterial community structure in soil by polymerase chain reaction and denaturing gradient gel electrophoresis.

    abstract::Bacterial community structure was studied in a Flevo silt loam (FSL) soil microplot, as well as in 15 other soils, by using DNA extraction followed by molecular fingerprinting. Total community DNA was extracted and purified by a direct method, which yielded amplifiable DNA of high molecular weight for all soils. A var...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章

    doi:10.1016/s0167-7012(99)00054-8

    authors: Gelsomino A,Keijzer-Wolters AC,Cacco G,van Elsas JD

    更新日期:1999-10-01 00:00:00

  • Pictures of microbiology -- the biofilm imaging facility under Dr. David C. White.

    abstract::This contribution honoring David C. White (DC) summarizes the five years I interacted with him on a daily basis in his laboratory. Over this time we worked on many different projects all tied together by the unifying principle now recognized as central to bacterial life in nature: biofilms. My goal is to convey some o...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章

    doi:10.1016/j.mimet.2007.07.003

    authors: Palmer RJ Jr

    更新日期:2008-07-01 00:00:00

  • A development and an improvement of selectable markers in Pleurotus ostreatus transformation.

    abstract::Pleurotus ostreatus was transformed using the nourseothricin-resistant gene for the first time. The transformation efficiency was 1.3±0.6transformants/μg plasmid DNA. In addition, the transformation efficiency of the bialaphos-resistant gene was increased to 26.7±11.5transformants/μg plasmid DNA. ...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章

    doi:10.1016/j.mimet.2017.01.007

    authors: Matsunaga Y,Ando M,Izumitsu K,Suzuki K,Honda Y,Irie T

    更新日期:2017-03-01 00:00:00

  • Comparison of culture and biochemical tests with PCR for detection of Brachyspira hyodysenteriae and Brachyspira pilosicoli.

    abstract::Traditional culture and biochemical tests (CBT) were compared with PCR for sensitivity and detection of Brachyspira hyodysenteriae and Brachyspira pilosicoli in seeded faeces and clinical samples from diarrhoeic pigs. A duplex PCR system was developed based on primers detecting the tlyA-gene of B. hyodysenteriae and t...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章

    doi:10.1016/j.mimet.2005.12.008

    authors: Råsbäck T,Fellström C,Gunnarsson A,Aspán A

    更新日期:2006-08-01 00:00:00

  • Development of a L-rhamnose and D-arabitol supplemented MacConkey agar to identify pathogenic Yersinia enterocolitica among environmental Yersinias in swine production wastes.

    abstract::Supplemented MacConkey agar with L-rhamnose and D-arabitol distinguishes pathogenic from environmental strains of Yersinia enterocolitica recovered from swine production wastes. This medium has a sensitivity of 100% and a specificity of 97.4%. ...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章

    doi:10.1016/j.mimet.2004.01.014

    authors: Shehee MW,Sobsey MD

    更新日期:2004-05-01 00:00:00

  • Bacterial identification using a SCIEX 5800 TOF/TOF MALDI research instrument and an external database.

    abstract::In our current study we were identifying 26 bacterial isolates using a SCIEX 5800 TOF/TOF MALDI instrument and an external database. The results were compared with the results of a Vitek® MS system and in case of discrepancies at the species level 16s rRNA sequencing was performed for further verification. ...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章

    doi:10.1016/j.mimet.2019.105685

    authors: Huber CA,Pflüger V,Reed S,Cottrell K,Sidjabat HE,Ranasinghe A,Zowawi HM,Harris P,Paterson DL

    更新日期:2019-09-01 00:00:00

  • A colorimetric microtiter plate method for assessment of phage effect on Pseudomonas aeruginosa biofilm.

    abstract::Bacteriophages have a potential in biofilm control. The aim of the study was to develop a method for selection of the most effective Pseudomonas aeruginosa phages for inhibition of biofilm formation and its eradication. The microtiter plate method is based on crystal violet staining and measuring of optical density. ...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章

    doi:10.1016/j.mimet.2008.03.005

    authors: Knezevic P,Petrovic O

    更新日期:2008-08-01 00:00:00

  • Successful Leptospira genotyping strategy on DNA extracted from canine biological samples.

    abstract::Leptospirosis is an emerging worldwide zoonosis with a changing epidemiology responsible for an acute disease in humans and dogs. A better knowledge of the responsible bacterium Leptospira and in particular its various serovars and serogroups prevalence is essential for better diagnosis and prevention of the disease. ...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章

    doi:10.1016/j.mimet.2020.106007

    authors: Le Guyader M,Fontana C,Simon-Dufay N,Balzer HJ,Pantchev N,Thibault JC,Cupillard L,Bomchil N,Kodjo A

    更新日期:2020-09-01 00:00:00

  • Analysis of major band of Enterobacter sakazakii by ERIC-PCR and development of a species-specific PCR for detection of Ent. sakazakii in dry food samples.

    abstract::ERIC (Enterobacterial Repetitive Intergenic Consensus)-PCR was employed to generate stable and reproductive ERIC-PCR fingerprints of Ent. sakazakii ATCC51329. Moreover, this study also cloned and sequenced a major band of Ent. sakazakii (ATCC51329) ERIC-PCR fingerprints. The major band was amplified with primer ERIC2 ...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章

    doi:10.1016/j.mimet.2008.07.012

    authors: Ye Y,Wu Q,Zhou Y,Dong X,Zhang J

    更新日期:2008-12-01 00:00:00

  • Live imaging of the genetically intractable obligate intracellular bacteria Orientia tsutsugamushi using a panel of fluorescent dyes.

    abstract::Our understanding of the molecular mechanisms of bacterial infection and pathogenesis are disproportionally derived from a small number of well-characterised species and strains. One reason for this is the enormous time and resources required to develop a new organism into experimental system that can be interrogated ...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章

    doi:10.1016/j.mimet.2016.08.022

    authors: Atwal S,Giengkam S,VanNieuwenhze M,Salje J

    更新日期:2016-11-01 00:00:00

  • Fine visualization of filamentous structures in the bacterial cytoplasm.

    abstract::A new method was established for fine visualization of bacterial subcelluar filamentous structures by freezing the bacterial cells to displace cytoplasmic matrix granules to the periphery. This method was successfully applied in immunoelectron microscopy and electron microscopic tomography, and should be applicable fo...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章

    doi:10.1016/j.mimet.2012.03.016

    authors: Nakano T,Aoki H,Wu H,Fujioka Y,Nakazawa E,Sano K

    更新日期:2012-07-01 00:00:00

  • Use of viability staining in combination with flow cytometry for rapid viability assessment of Lactobacillus rhamnosus GG in complex protein matrices.

    abstract::The aim of this study was to demonstrate that flow cytometry (FACS) could potentially be employed for rapid viability assessment of probiotic bacteria immobilized or encapsulated in complex matrices. Lactobacillus rhamnosus GG was immobilized within six different protein environments using whey protein isolate (WPI) a...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章

    doi:10.1016/j.mimet.2010.07.003

    authors: Doherty SB,Wang L,Ross RP,Stanton C,Fitzgerald GF,Brodkorb A

    更新日期:2010-09-01 00:00:00

  • Hydrophysical correlation and water mass indication of optical physiological parameters of picophytoplankton in Prydz Bay during autumn 2008.

    abstract::Flow cytometry (FCM) is efficient in detecting both abundance and optical physiological parameters including cell size and cellular carbon content-side scatter (SSC), carotenoids-green and orange fluorescence (FL1 and FL2), and red fluorescence-chlorophylls (FL3) can be obtained by FCM. The utilization of these physio...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章

    doi:10.1016/j.mimet.2012.09.030

    authors: Zhang F,Ma Y,Lin L,He J

    更新日期:2012-12-01 00:00:00

  • Sterilization of biological pathogens using supercritical fluid carbon dioxide containing water and hydrogen peroxide.

    abstract::Novel noninvasive techniques for the removal of biological contaminants to generate clean or sterile materials are in demand by the medical, pharmaceutical and food industries. The sterilization method described here uses supercritical fluid carbon dioxide (SF-CO(2)) containing 3.3% water and 0.1% hydrogen peroxide (v...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章

    doi:10.1016/j.mimet.2011.07.008

    authors: Checinska A,Fruth IA,Green TL,Crawford RL,Paszczynski AJ

    更新日期:2011-10-01 00:00:00

  • Immobilization in alginate as a technique for the preservation of Bacillus thuringiensis var. israelensis for long-term preservation.

    abstract::Technique for immobilization using sodium alginate as the matrix to preserve Bacillus thuringiensis var. israelensis isolates for long time storage was developed. Two strains of B. thuringiensis var. israelensis viz., VCRC B-17 and WHO standard strain IPS-82 were immobilized in alginate matrix and preserved at 4 degre...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章

    doi:10.1016/j.mimet.2007.11.001

    authors: Prabakaran G,Hoti SL

    更新日期:2008-01-01 00:00:00

  • Isolation of Harveyi clade Vibrio spp. collected in aquaculture farms: How can the identification issue be addressed?

    abstract::Aquaculture is a fast growing industry with its development hampered by bacterial diseases. Vibriosis caused by Harveyi clade strains is known for causing heavy loss especially in shrimp aquaculture farms. For farm treatment and pathogen spread management, veterinarians and researchers need reliable bacterial identifi...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章

    doi:10.1016/j.mimet.2020.106106

    authors: Culot A,Grosset N,Bruey Q,Auzou M,Giard JC,Favard B,Wakatsuki A,Baron S,Frouel S,Techer C,Gautier M

    更新日期:2021-01-01 00:00:00

  • Mass spectrometry and tandem mass spectrometry characterization of protein patterns, protein markers and whole proteomes for pathogenic bacteria.

    abstract::There have been many recent reviews published on MALDI-TOF MS (matrix assisted laser desorption/ionization time-of-flight) MS (mass spectrometry) for identification of bacteria particularly with relevance to clinical microbiology. MALDI-TOF MS is now a mature technique for bacterial identification with great promise. ...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章,评审

    doi:10.1016/j.mimet.2013.01.004

    authors: Intelicato-Young J,Fox A

    更新日期:2013-03-01 00:00:00

  • Biotransformation of 12-hydroxystearic acid to gamma-decalactone: Comparison of two separation systems.

    abstract::Biotransformation of natural products to the natural flavoring, gamma-decalactone (GDL), has attracted considerable attention. However, improving its yield is challenging due to its high feedback inhibition of yeast cells, which lowers the productivity of the biotransformation process. In this study, we compared two i...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章

    doi:10.1016/j.mimet.2020.106041

    authors: Rong S,Guan X,Li Q,Guan S,Cai B,Zhang S

    更新日期:2020-09-03 00:00:00

  • An evaluation of the impact of clinical bacterial isolates on epithelial cell monolayer integrity by the electric Cell-Substrate Impedance Sensing (ECIS) method.

    abstract::Virulence is the relative capacity of a pathogenic microorganism to cause damage in susceptible host cells such as those found in airway passages and the gut. In this study, the effect of clinical bacterial isolates on the monolayer integrity of cultured human alveolar basal epithelial cells (A549) was evaluated using...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章

    doi:10.1016/j.mimet.2020.105833

    authors: Nahid MA,Campbell CE,Fong KSK,Barnhill JC,Washington MA

    更新日期:2020-02-01 00:00:00

  • PCR procedures to amplify GC-rich DNA sequences of Mycobacterium bovis.

    abstract::Amplification of high GC content genes by PCR is a major challenge during the creation of recombinant GC-rich DNA constructs. This may be due to the difficulty in DNA denaturation or the possibility of forming secondary structures from DNA templates. Tools have been described to address the technical problems associat...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章

    doi:10.1016/j.mimet.2020.106121

    authors: Assal N,Lin M

    更新日期:2020-12-13 00:00:00

  • Permeabilization and hybridization protocols for rapid detection of Bacillus spores using fluorescence in situ hybridization.

    abstract:BACKGROUND:Fluorescence in situ hybridization (FISH) is not adapted for the detection of bacterial spores because of their resistance to conventional permeabilization treatments. Since spore-forming bacteria have important ecological, economical, and medical roles, their in situ detection needs to be improved. The aim ...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章

    doi:10.1016/j.mimet.2008.12.009

    authors: Filion G,Laflamme C,Turgeon N,Ho J,Duchaine C

    更新日期:2009-04-01 00:00:00

  • Detection of respiratory enzyme activity in Giardia cysts and Cryptosporidium oocysts using redox dyes and immunofluorescence techniques.

    abstract::The fluorescent redox dye 5-cyano-2,3-ditolyl tetrazolium chloride (CTC), combined with fluorescein-labeled antibodies, was tested for the simultaneous detection of the respiratory electron transport system (ETS) activity and enumeration of Giardia cysts and Cryptosporidium oocysts by spectral microfluorometry and epi...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章

    doi:10.1016/s0167-7012(01)00249-4

    authors: Iturriaga R,Zhang S,Sonek GJ,Stibbs H

    更新日期:2001-07-30 00:00:00

  • Optimal methods for evaluating antimicrobial activities from plant extracts.

    abstract::The search for antimicrobial agents from plants has been a growing interest in the last few decades. However, results generated from many of these studies cannot be directly compared due to the absence of standardization in particular antimicrobial methods employed. The need for established methods with consistent res...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章

    doi:10.1016/j.mimet.2010.11.008

    authors: Othman M,Loh HS,Wiart C,Khoo TJ,Lim KH,Ting KN

    更新日期:2011-02-01 00:00:00