A strategy to verify the absence of the pgm locus in Yersinia pestis strain candidates for select agent exemption.

Abstract:

:Yersinia pestis is a Department of Health and Human Services select agent as defined in federal regulations. Certain attenuated strains of Y. pestis, such as the pgm(-) strain, are exempt from these regulations. Herein we describe a strategy to verify the absence of the pgm locus in Y. pestis strains being considered as candidates for select agent exemption by PCR analysis of virulence-associated genes.

journal_name

J Microbiol Methods

authors

Jenkins AL,Worsham PL,Welkos SL

doi

10.1016/j.mimet.2009.02.013

subject

Has Abstract

pub_date

2009-06-01 00:00:00

pages

316-9

issue

3

eissn

0167-7012

issn

1872-8359

pii

S0167-7012(09)00069-4

journal_volume

77

pub_type

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