abstract：:Fluorescence in situ hybridization (FISH) can provide information on the morphology, spatial arrangement, and local environment of individual cells enabling the investigation of intact microbial communities. GeneFISH uses polynucleotide probes and enzymatic signal amplification to detect genes that are present in low ...

journal_title：Journal of microbiological methods

authors： Richards PM,Mattes TE

更新日期：2021-01-22 00:00:00

abstract：:Acidobacteriota are highly abundant in soils, however, few cultured representatives are available. The purity of the reagents can influence microbial growth in laboratory conditions and successful isolation. Here we investigated the impact of different agar brands in culture medium and advocate that agar origin should...

journal_title：Journal of microbiological methods

authors： Costa OYA,Kuramae EE

更新日期：2021-01-20 00:00:00

abstract：:Brucella melitensis and Brucella abortus account for almost all cases of brucellosis in Turkish population. We developed a fourplex quantitative real-time PCR (qPCR) assay for the electrophoresis-free, rapid and cost-effective differentiation of B. abortus and B. melitensis from the other Brucella spp. The 4-plex spec...

journal_title：Journal of microbiological methods

authors： Kılıç S,Çelebi B,Turan M

更新日期：2021-01-01 00:00:00

abstract：:Aquaculture is a fast growing industry with its development hampered by bacterial diseases. Vibriosis caused by Harveyi clade strains is known for causing heavy loss especially in shrimp aquaculture farms. For farm treatment and pathogen spread management, veterinarians and researchers need reliable bacterial identifi...

journal_title：Journal of microbiological methods

authors： Culot A,Grosset N,Bruey Q,Auzou M,Giard JC,Favard B,Wakatsuki A,Baron S,Frouel S,Techer C,Gautier M

更新日期：2021-01-01 00:00:00

abstract：:Rapid identification of bacterial pathogens facilitates earlier optimization of antibiotic treatment and reduces morbidity and mortality in sepsis patients. The aim of this research was to design an in-house chemical-free method for direct bacterial identification in positive blood culture (BC) broths and to compare t...

journal_title：Journal of microbiological methods

authors： Ko YJ,Kook JK,Lee CK

更新日期：2021-01-01 00:00:00

abstract：:Amplification of high GC content genes by PCR is a major challenge during the creation of recombinant GC-rich DNA constructs. This may be due to the difficulty in DNA denaturation or the possibility of forming secondary structures from DNA templates. Tools have been described to address the technical problems associat...

journal_title：Journal of microbiological methods

authors： Assal N,Lin M

更新日期：2020-12-13 00:00:00

abstract：BACKGROUND:Mycoplasma genitalium resistance to antibiotic treatments is increasing, with very limited treatment alternatives on the horizon. Surveillance via sequencing of multiple M. genitalium loci would allow: monitoring of known antibiotic resistance mutations, associations between resistance/treatment failure and ...

journal_title：Journal of microbiological methods

authors： Plummer EL,Murray GL,Bodiyabadu K,Su J,Garland SM,Bradshaw CS,Read TRH,Tabrizi SN,Danielewski JA

更新日期：2020-12-01 00:00:00

abstract：:Whole-cell bacterial biosensors can be applied for the screening of antibacterial properties of extracts. We constructed a biosensor panel consisting of four different bacterial biosensor strains: Escherichia, Staphylococcus, Acinetobacter and Pseudomonas for expanded screening potential. The functionality of the pane...

journal_title：Journal of microbiological methods

authors： Poikulainen E,Tienaho J,Sarjala T,Santala V

更新日期：2020-10-14 00:00:00

abstract：:Anthrax is one of the important diseases in humans and animals, caused by the gram-positive bacteria spores called Bacillus anthracis. The disease is still one of the health problems of developing countries. Due to fatigue and decreased visual acuity, microscopic diagnosis of diseases by humans may not be of good qual...

journal_title：Journal of microbiological methods

authors： Hoorali F,Khosravi H,Moradi B

更新日期：2020-10-01 00:00:00

abstract：:We prepared a newer growth medium, banana peel extract agar (BPEA) containing the extracts of chopped banana peels for the selective cultivation of Cryptococcus neoformans. Over the medium, the growth resulted in the development of light to the dark brown coloured colonies indicating the chromogenic potential of the B...

journal_title：Journal of microbiological methods

authors： Gupta MK,Mallick P,Pandey N,Shankar V,Chakravarty J,Tilak R

更新日期：2020-10-01 00:00:00

abstract：:Long-read sequencing technologies are having a major impact on our approaches to studying non-model organisms and microbial communities. By significantly reducing the cost and facilitating the genome assembly pipelines, any laboratory can now develop its own genomics program regardless of the complexity of the genome ...

journal_title：Journal of microbiological methods

authors： Penouilh-Suzette C,Fourré S,Besnard G,Godiard L,Pecrix Y

更新日期：2020-09-12 00:00:00

abstract：:Biotransformation of natural products to the natural flavoring, gamma-decalactone (GDL), has attracted considerable attention. However, improving its yield is challenging due to its high feedback inhibition of yeast cells, which lowers the productivity of the biotransformation process. In this study, we compared two i...

journal_title：Journal of microbiological methods

authors： Rong S,Guan X,Li Q,Guan S,Cai B,Zhang S

更新日期：2020-09-03 00:00:00

abstract：:An acute hepatopancreatic necrosis disease (AHPND) causes serious losses to the global shrimp industry. The etiologic agent of AHPND is Vibrio spp. carrying a large plasmid which encodes a binary toxin, PirAB. Currently, AHPND is diagnosed by PCR based methods that detect the presences of both pirA and pirB genes. How...

journal_title：Journal of microbiological methods

authors： Mai HN,Cruz-Flores R,Dhar AK

更新日期：2020-09-01 00:00:00

abstract：:The objective of this study was to evaluate the effects of increased PCR cycle number on sequencing results from samples with low microbial biomass, including bovine milk, and murine pelage and blood. We hypothesized that subjecting DNA from such samples to higher PCR cycle numbers would increase 16S rRNA sequencing c...

journal_title：Journal of microbiological methods

authors： Witzke MC,Gullic A,Yang P,Bivens NJ,Adkins PRF,Ericsson AC

更新日期：2020-09-01 00:00:00

abstract：:This report describes the development, optimization, and validation of a ddPCR assay for the detection of Bartonella spp. DNA within several sample matrices, including clinical blood samples from patients with or without documented Bartonella spp. bacteremia. The Bartonella spp. ddPCR assay was developed based upon pr...

journal_title：Journal of microbiological methods

authors： Maggi RG,Richardson T,Breitschwerdt EB,Miller JC

更新日期：2020-09-01 00:00:00

abstract：:Leptospirosis is an emerging worldwide zoonosis with a changing epidemiology responsible for an acute disease in humans and dogs. A better knowledge of the responsible bacterium Leptospira and in particular its various serovars and serogroups prevalence is essential for better diagnosis and prevention of the disease. ...

journal_title：Journal of microbiological methods

authors： Le Guyader M,Fontana C,Simon-Dufay N,Balzer HJ,Pantchev N,Thibault JC,Cupillard L,Bomchil N,Kodjo A

更新日期：2020-09-01 00:00:00

abstract：:The latex agglutination test using single-chain antibody fragments (scFvStx1 and scFvStx2) coupled to latex particles, was compared with the gold standard Vero cell assay for Shiga toxin (Stx) detection, aiming to estimate the diagnosis potential of these scFv fragments in a rapid and straightforward test. The latex c...

journal_title：Journal of microbiological methods

authors： Shiga EA,Guth BEC,Piazza RMF,Luz D

更新日期：2020-08-01 00:00:00

abstract：:Preservation of strains is important to further studies and characterization; we have shown a technique of long-term preservation of Neisseria spp. and Haemophilus spp. by freezing cotton tip swabs impregnated with fastidious organisms at -80 °C without the addition of cryoprotectants. ...

journal_title：Journal of microbiological methods

authors： Villa L,Torreblanca A,Otero L,Carreño F,Campo R,López-Escobar M,Vazquez F

更新日期：2020-08-01 00:00:00

abstract：:Paper-based nucleic acid testing techniques are increasingly in demand. Hence, we have developed a simple and cheap paper fluidic device to detect multiple gene targets in Leptospira. Fluidic channels of the penta-clover device are drawn using a correction pen on Whatman filter paper 1. The fluid blocks the pores of t...

journal_title：Journal of microbiological methods

authors： Varsha V,Aishwarya S,Murchana S,Naveen G,Ramya M,Rathinasabapathi P

更新日期：2020-07-01 00:00:00

abstract：:Lactic acid bacteria (LAB) play important roles in the food industry, animal husbandry and medicine which are closely related to human life. Modern gene engineering technology is the major means to reveal gene functions, study metabolic pathways and metabolomes of strains and improve the properties of strains. However...

journal_title：Journal of microbiological methods

authors： Wang C,Cui Y,Qu X

更新日期：2020-07-01 00:00:00

abstract：:Brucellosis is a zoonotic disease transmitted to humans from infected animals. As a systemic disease, it can harm any organ or system of the host body. Human brucellosis presents with various clinical symptoms, which makes diagnosis challenging. Serological diagnosis of brucellosis is based on ELISA or agglutination t...

journal_title：Journal of microbiological methods

authors： Yin D,Bai Q,Zhang J,Xu K,Li J

更新日期：2020-07-01 00:00:00

abstract：:DNA-SIP (DNA-based stable isotope probing) is increasingly being employed in soil microbial ecology to identify those microbes assimilating the 13C/15N labelled substrate. Isopycnic gradient centrifugation is the primary experimental process for conducting DNA-SIP. However, diverse centrifugal conditions have been use...

journal_title：Journal of microbiological methods

authors： Wang J,Zhang X,Yao H

更新日期：2020-06-01 00:00:00

abstract：:To prevent postoperative infectious complications, a urinary tract infection should be either diagnostically excluded or treated prior to ureterorenoscopy (URS). URS is a frequently performed endoscopic surgery for urological stone removal. Although the urinary dipstick test represents a simple and cost-effective meth...

journal_title：Journal of microbiological methods

authors： Grüne B,Viehweger V,Waldbillig F,Nientiedt M,Kriegmair MC,Rassweiler-Seyfried MC,Ritter M

更新日期：2020-06-01 00:00:00

abstract：:Fusarium guttiforme and Fusarium ananatum are the etiological agents of fusariosis and fruitlet core rot in pineapple, respectively, producing mycotoxins that are harmful to the health of consumers. These two fungi are morphologically similar and difficulty in obtaining macroconidia of the species limits their identif...

journal_title：Journal of microbiological methods

authors： Moura RD,de Castro LAM,Culik MP,Fernandes AAR,Fernandes PMB,Ventura JA

更新日期：2020-06-01 00:00:00

abstract：:Many methods are developed to assess antimicrobial action of ZnO nanoparticles (NPs). A large number of methods associated with the use of fluorescent probes are developed, including Spectrofluorometry, fluorescence microscopy, and cytometry. In this study, flowcytometry, Spectrofluorometry and fluorescent microscopy ...

journal_title：Journal of microbiological methods

authors： Khater M,Khater SS,Gholap H,Patil R,Kulkarni G

更新日期：2020-06-01 00:00:00

abstract：:To establish a simple and rapid high-resolution melting curve (HRM) method, 5 different strains of Salmonella were identified by adding DNA denaturants at different concentrations into the HRM system to change the characteristics of DNA melting and to obtain different Tm (dissolving temperature) values of DNA from dif...

journal_title：Journal of microbiological methods

authors： Hu M,Yang D,Wu X,Luo M,Xu F

更新日期：2020-05-01 00:00:00

abstract：:Any forms of valorization of microorganisms would require accurate identity recognition to ensure repeatability, reproducibility and quality assurance. This study aimed to evaluate the effectiveness of different primers for identifying cultured eukaryotic microalgae using a simple 18S rDNA approach. A total of 34 isol...

journal_title：Journal of microbiological methods

authors： Khaw YS,Khong NMH,Shaharuddin NA,Yusoff FM

更新日期：2020-05-01 00:00:00

abstract：:The limited availability of biological samples hinders phylogenetic efforts to define structural differences among various biological groups. A novel workflow enabling the analysis of protists in low cell numbers by electron microscopy (EM) is described with cysts of Giardia intestinalis, a single-celled eukaryotic pa...

journal_title：Journal of microbiological methods

authors： Tůmová P,Nohýnková E,Klingl A,Wanner G

更新日期：2020-05-01 00:00:00

abstract：:Treatment with 70% ethanol has been proposed as a safe and effective way to inactive bacteria for transport between laboratories prior to identification by MALDI-TOF MS. Ethanol alone does not inactivate spore-forming bacteria and additional chemical or physical treatment is necessary to guarantee inactivation of bact...

journal_title：Journal of microbiological methods

authors： Palombo EA

更新日期：2020-05-01 00:00:00

abstract：:The skin microbiota is characterized by high intra- and inter-variability among individuals, due to a multitude of intrinsic and extrinsic parameters such as genetics, lifestyles or pollution. This variability may be heightened due to sampling method as the skin is a multilayer organ and its outermost layer consists o...

journal_title：Journal of microbiological methods

authors： Boulesnane Y,Leloup J,Lerch TZ,Roynette A,Pensé-Lhéritier AM,Mielcarek C,Changey F

更新日期：2020-04-01 00:00:00

abstract：:Uropathogenic Escherichia coli (UPEC) is the most common causal agent of urinary tract infections (UTIs) in humans. Currently, clinical detection methods take hours (dipsticks) to days (culturing methods), limiting rapid intervention. As an alternative, the use of molecular methods could improve speed and accuracy, bu...

journal_title：Journal of microbiological methods

authors： Brons JK,Vink SN,de Vos MGJ,Reuter S,Dobrindt U,van Elsas JD

更新日期：2020-02-01 00:00:00

abstract：:Virulence is the relative capacity of a pathogenic microorganism to cause damage in susceptible host cells such as those found in airway passages and the gut. In this study, the effect of clinical bacterial isolates on the monolayer integrity of cultured human alveolar basal epithelial cells (A549) was evaluated using...

journal_title：Journal of microbiological methods

authors： Nahid MA,Campbell CE,Fong KSK,Barnhill JC,Washington MA

更新日期：2020-02-01 00:00:00

abstract：:Watermelon peel waste (WPW) is a zero value material containing a high amount of nutrients that could be utilized in microbial growth medium production. In the present work, WPW was used to produce universal fungal growth medium with great nutritional profile to use instead of other expensive media particularly the Po...

journal_title：Journal of microbiological methods

authors： Hasanin MS,Hashem AH

更新日期：2020-01-01 00:00:00

abstract：:Neisseria gonorrhoeae (NG) has developed resistance to most antibiotics, making it increasingly difficult to treat. Previous studies have predicted antimicrobial NG susceptibility based on the antimicrobial gene target DNA gyrase subunit A (gyrA) codon serine 91 and the penicillin-binding protein 2 (penA) using Roche ...

journal_title：Journal of microbiological methods

authors： Qadir S,Ellis O,Keizur EM,Stafylis C,Cortado R,Klausner JD,Adolescent Trials Network (ATN) CARES Team.

更新日期：2020-01-01 00:00:00

abstract：:Bacterial endosymbionts such as Rickettsia and Wolbachia play prominent roles in the development and behaviour of their insect hosts, such as whiteflies, aphids, psyllids and mealybugs. Accumulating studies have emphasized the importance of establishing experimental insect populations that are either lacking or bearin...

journal_title：Journal of microbiological methods

authors： Liu Y,Fan ZY,An X,Shi PQ,Ahmed MZ,Qiu BL

更新日期：2020-01-01 00:00:00

abstract：:Nanoparticles synthesized through the green route deserve special mention because this green technology is not only energy-efficient and cost-effective but also amenable to the environment. Various biological resources have been used for the generation of these 'green nanoparticles'. Biological wastes have also been f...

journal_title：Journal of microbiological methods

authors： Das P,Karankar VS

更新日期：2019-12-01 00:00:00

abstract：:Rapid and sensitive detection of extended-spectrum β-lactamases (ESBLs) is essential for infection control and antimicrobial treatment. Recently, a matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS)-based MBT STAR-BL software module has been used for detecting β-lactamase activ...

journal_title：Journal of microbiological methods

authors： Ota Y,Furuhashi K,Nagao Y,Nanba T,Yamanaka K,Ishikawa J,Nagura O,Iwaizumi M,Hamada E,Maekawa M

更新日期：2019-12-01 00:00:00

abstract：:Staphylococcal Cassette Chromosome mec (SCCmec) element plays a key role in development of resistance by carrying different resistance factors. Therefore, routine and rapid diagnostic approach is considered advantageous for the easy detection of SCCmec elements. So, here we have described the use of three sets of mult...

journal_title：Journal of microbiological methods

authors： Bhowmik D,Das BJ,Pandey P,Chetri S,Chanda DD,Bhattacharjee A

更新日期：2019-11-01 00:00:00

abstract：:Leishmaniasis, caused by protozoa belonging to the genus Leishmania, is an important public health problem found in >90 countries and with still limited options for treatment. Development of new anti-leishmanial drugs is an urgent need and the identification of new active compounds is a limiting factor that can be acc...

journal_title：Journal of microbiological methods

authors： da Silva Santos AC,Moura DMN,Dos Santos TAR,de Melo Neto OP,Pereira VRA

更新日期：2019-11-01 00:00:00

abstract：:Industrial biotechnology aims to compete as a stronger alternative ensuring environmental friendly microbial-based production that seeks to curb the predicament of pollution. However, the high cost of bioprocessing is a severe drawback, and therefore, new approaches must be developed to overcome this challenge. Haloph...

journal_title：Journal of microbiological methods

authors： Liu C,Baffoe DK,Zhan Y,Zhang M,Li Y,Zhang G

更新日期：2019-11-01 00:00:00