Abstract:
:Leishmaniasis is a disease, caused by Leishmania parasites, which infect humans and animals, posing a major social and economic burden worldwide. The need for accurate and sensitive disease diagnosis led to the widespread adoption of PCR amplification. Detection of the amplification products (i.e. gel electrophoresis) require time-consuming protocols performed by trained personnel, with high cost. Aim of the present study was the simplification of PCR product detection, using a nucleic acid lateral flow, combined with functionalized gold nanoparticles. Amplification reactions targeting kinetoplastid DNA of Leishmania spp were performed on canine blood samples and a positive signal was formed as a red test zone. The visual detection was completed in 20min. Extensive optimization enabled the detection of 100fmol of target DNA. Clinical samples of infected dog blood were analyzed with high specificity. Overall, the proposed lateral flow biosensor can be considered an appealing alternative platform for Leishmania-specific amplification products detection with low cost and attractive simplicity.
journal_name
J Microbiol Methodsjournal_title
Journal of microbiological methodsauthors
Toubanaki DK,Athanasiou E,Karagouni Edoi
10.1016/j.mimet.2016.05.027subject
Has Abstractpub_date
2016-08-01 00:00:00pages
51-58eissn
0167-7012issn
1872-8359pii
S0167-7012(16)30124-5journal_volume
127pub_type
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