当前位置： SCI文献检索 > JOURNAL OF MICROBIOLOGICAL METHODS期刊下所有文献 > Comparison of procedures for the extraction of supernatants and cytotoxicity tests in Vero cells, applied to assess the toxigenic potential of Bacillus spp. and Lactobacillus spp., intended for use as probiotic strains.

Comparison of procedures for the extraction of supernatants and cytotoxicity tests in Vero cells, applied to assess the toxigenic potential of Bacillus spp. and Lactobacillus spp., intended for use as probiotic strains.

Abstract：

:Interest in using Bacillus strains as probiotic components of animal feeds has grown in recent years. However, some of these strains, especially those taxonomically related to the Bacillus cereus group, may have enterotoxigenic activity. Assessment of their toxigenic potential by well-established and robust protocols is required before authorizing their use in animal nutrition. Three methods of extraction and concentration of supernatants of Bacillus and Lactobacillus strains (methanol extraction, ammonium sulphate and ultrafiltration concentration) and three cytotoxic tests in Vero cells (WST-1, LDH and protein synthesis inhibition assays) for the assessment of the cytotoxicity activity of Lactobacillus strains (as probiotic strains in human and animal nutrition) and Bacillus toyonensis BCT-7112(T) (as animal probiotic strain in animal nutrition-Toyocerin®-) were evaluated in this study. Methanol extraction was not useful under any circumstances. The other two concentration methods (ammonium sulphate and ultrafiltration) were feasible, with slightly greater sensitivity achieved by ultrafiltration. The probiotic strain B. toyonensis BCT-7112(T) proved to be a non-cytotoxic strain in all the protocols tested. However, some Lactobacillus strains showed cytotoxicity activity, regardless of the protocols applied.

journal_name

J Microbiol Methods

journal_title

Journal of microbiological methods

authors

Blanch AR,Méndez J,Castel S,Reina M

doi

10.1016/j.mimet.2014.05.019

subject

Has Abstract

pub_date

2014-08-01 00:00:00

pages

64-9

eissn

0167-7012

issn

1872-8359

pii

S0167-7012(14)00149-3

journal_volume

103

pub_type

杂志文章

相关文献

JOURNAL OF MICROBIOLOGICAL METHODS文献大全
  • A PCR-based method to quantify fungal growth during pretreatment of lignocellulosic biomass.

    abstract：:Filamentous fungi have shown great potential in the pretreatment of lignocellulosic biomass and their use in bio-processes based on Solid State Fermentation (SSF) opens promising perspectives for plant biomass valorization. Obviously, quantification of the fungal biomass throughout the fermentation is a crucial parame...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2015.05.024

    authors： Simeng Z,Sacha G,Isabelle HG,Marie-Noëlle R

    更新日期：2015-08-01 00:00:00

  • Dead-end ultrafiltration concentration and IMS/ATP-bioluminescence detection of Escherichia coli O157:H7 in recreational water and produce wash.

    abstract：:The purpose of this study was to develop a detection method for viable E. coli O157:H7 in fresh produce and recreational water. The method was evaluated using eight samples of produce wash and recreational water with or without spiked E. coli O157:H7 at ≤10(2) CFU·ml(-1) and concentrated using dead-end ultrafiltration...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2011.09.010

    authors： Hunter DM,Leskinen SD,Magaña S,Schlemmer SM,Lim DV

    更新日期：2011-12-01 00:00:00

  • Numerical solution of a microbial growth model applied to dynamic environments.

    abstract：:The Baranyi and Roberts model is one of the most frequently used microbial growth models. It has been successfully applied to numerous studies of various microorganisms in different food products. Under dynamic conditions, the model is implicitly formulated as a set of two coupled differential equations which could be...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2015.03.003

    authors： Zhu S,Chen G

    更新日期：2015-05-01 00:00:00

  • Engineered bakers yeast as a sensitive bioassay indicator organism for the trichothecene toxin deoxynivalenol.

    abstract：:The aim of this study was to increase the sensitivity of Saccharomyces cerevisiae towards trichothecene toxins, in particular to deoxynivalenol (DON), in order to improve the utility of this yeast as a bioassay indicator organism. We report the construction of a strain with inactivated genes (PDR5, PDR10, PDR15) encod...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2007.12.013

    authors： Abolmaali S,Mitterbauer R,Spadiut O,Peruci M,Weindorfer H,Lucyshyn D,Ellersdorfer G,Lemmens M,Moll WD,Adam G

    更新日期：2008-03-01 00:00:00

  • Investigations of structure and metabolism within Shewanella oneidensis MR-1 biofilms.

    abstract：:Biofilms possess spatially and temporally varying metabolite concentration profiles at the macroscopic and microscopic scales. This results in varying growth environments that may ultimately drive species diversity, determine biofilm structure and the spatial distribution of the community members. Using non-invasive n...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2008.02.015

    authors： McLean JS,Majors PD,Reardon CL,Bilskis CL,Reed SB,Romine MF,Fredrickson JK

    更新日期：2008-07-01 00:00:00

  • Optimization of media components for production of antimicrobial compound by Brevibacillus brevis EGS9 isolated from mangrove ecosystem.

    abstract：:The aim of the present study was to show the efficiency of multi-factorial experimental design to investigate factors affecting the antimicrobial compound production by Brevibacillus brevis EGS9 isolated from mangrove sediment soil. The isolate utilizing different carbon and nitrogen sources showed different growth ch...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2017.09.010

    authors： T A,P SK

    更新日期：2017-11-01 00:00:00

  • Improved pH-ratiometry for the three-dimensional mapping of pH microenvironments in biofilms under flow conditions.

    abstract：:Confocal microscopy-based monitoring of pH in biofilms is gaining increasing interest, as it allows for a quick assessment of horizontal pH gradients without mechanically perturbing the biofilm. Ratiometric monitoring of pH with the fluorescent dye C-SNARF-4 has been used to reliably map extracellular pH in the basal ...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2018.08.007

    authors： Schlafer S,Baelum V,Dige I

    更新日期：2018-09-01 00:00:00

  • Evaluation of urine for Leishmania infantum DNA detection by real-time quantitative PCR.

    abstract：:The availability of some sorts of biological samples which require noninvasive collection methods has led to an even greater interest in applying molecular biology on visceral leishmaniasis (VL) diagnosis, since these samples increase the safety and comfort of both patients and health professionals. In this context, t...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2016.10.002

    authors： Pessoa-E-Silva R,Mendonça Trajano-Silva LA,Lopes da Silva MA,da Cunha Gonçalves-de-Albuquerque S,de Goes TC,Silva de Morais RC,Lopes de Melo F,de Paiva-Cavalcanti M

    更新日期：2016-12-01 00:00:00

  • Improved biovar test for Ralstonia solanacearum.

    abstract：:Race 3, biovar 2 strains of Ralstonia solanacearum are quarantined pathogens in Europe and Canada and Select Agent pathogens in the United States. The biovar classification of R. solanacearum strains is based on their biochemical abilities to utilize a carbohydrate panel. The standard biovar test uses bromothymol blue...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2011.12.007

    authors： Huang Q,Yan X,Wang JF

    更新日期：2012-02-01 00:00:00

  • Optimization of processing conditions for the quantification of enterococci biofilms using microtitre-plates.

    abstract：:We performed a comparison of four selected protocols from the literature using microtitre-plate and colorimetric biomass assay for evaluation of enterococci biofilm formation ability and optimized a protocol after the identification and stringent performance of biofilm formation steps. The optimized protocol uses a dy...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2010.11.007

    authors： Extremina CI,Costa L,Aguiar AI,Peixe L,Fonseca AP

    更新日期：2011-02-01 00:00:00

  • A selective medium for the isolation of Corynebacterium species in oral cavities.

    abstract：:Corynebacterium matruchotii is a microbial inhabitant in the oral cavity of humans and is associated with the formation of dental calculi. C. matruchotii forms highly specific morphological units, which are referred to as corn-cobs. Although other Corynebacterium species have frequently been isolated from the oral cav...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2014.06.005

    authors： Tsuzukibashi O,Uchibori S,Shinozaki-Kuwahara N,Kobayashi T,Takada K,Hirasawa M

    更新日期：2014-09-01 00:00:00

  • Subtle changes in host cell density cause a serious error in monitoring of the intracellular growth of Chlamydia trachomatis in a low-oxygen environment: Proposal for a standardized culture method.

    abstract：:We monitored Chlamydia trachomatis growth in HeLa cells cultured with either DMEM or RPMI medium containing 10% FCS under 2% or 21% O2 conditions for 2 days. Bacterial numbers, host cell numbers, and fibrosis-related gene expression in the host cells were estimated by an inclusion forming unit assay, a cell counting a...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2018.09.007

    authors： Sakai K,Matsuo J,Watanabe T,Okubo T,Nakamura S,Yamaguchi H

    更新日期：2018-10-01 00:00:00

  • Rapid detection of Haemophilus parasuis using cross-priming amplification and vertical flow visualization.

    abstract：:Haemophilus parasuis infection is of considerable economic importance in the swine industry due to high morbidity and mortality in naive swine populations. Accurate detection and identification of the causative agent are difficult, yet necessary, for disease control. In this study, a simple and rapid method of cross-p...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2017.11.005

    authors： Gou H,Li J,Cai R,Song S,Li M,Yang D,Jiang Z,Li Y,Chu P,Li C

    更新日期：2018-01-01 00:00:00

  • Optimal methods for evaluating antimicrobial activities from plant extracts.

    abstract：:The search for antimicrobial agents from plants has been a growing interest in the last few decades. However, results generated from many of these studies cannot be directly compared due to the absence of standardization in particular antimicrobial methods employed. The need for established methods with consistent res...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2010.11.008

    authors： Othman M,Loh HS,Wiart C,Khoo TJ,Lim KH,Ting KN

    更新日期：2011-02-01 00:00:00

  • Multiplex and quantifiable detection of nucleic acid from pathogenic fungi using padlock probes, generic real time PCR and specific suspension array readout.

    abstract：:A new concept for multiplex detection and quantification of microbes is here demonstrated on a range of infectious fungal species. Padlock probe methodology in conjunction with qPCR and Luminex technology was used for simultaneous detection of ten fungal species in one single experiment. By combining the multiplexing ...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2009.05.016

    authors： Eriksson R,Jobs M,Ekstrand C,Ullberg M,Herrmann B,Landegren U,Nilsson M,Blomberg J

    更新日期：2009-08-01 00:00:00

  • Modification of A-stat for the characterization of microorganisms.

    abstract：:Two novel modifications of continuous culture with gradual change of dilution rate (A-stat): D-stat and auxo-accelerostat were evaluated in the studies of the effect of changing individual environmental parameters (T, pH, pO(2), substrate concentration, etc.) on growth characteristics of different microorganisms. Comm...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/s0167-7012(03)00143-x

    authors： Kasemets K,Drews M,Nisamedtinov I,Adamberg K,Paalme T

    更新日期：2003-10-01 00:00:00

  • Highly efficient transformation of a (hemi-)cellulases-producing fungus Eupenicillium parvum 4-14 by Agrobacterium tumefaciens.

    abstract：:The mesophilic fungus Eupenicillium parvum 4-14 is an important producer of thermotolerant hemicellulolytic and cellulolytic enzymes. The aim of this study was to establish a method for genetic manipulation of the fungus by Agrobacterium tumefaciens. The promotor PgpdA of a glyceraldehyde-3-phosphate dehydrogenase gen...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2018.01.013

    authors： Long L,Lin Q,Shi Y,Wang J,Ding S

    更新日期：2018-03-01 00:00:00

  • Rapid and simple detection of food poisoning bacteria by bead assay with a microfluidic chip-based system.

    abstract：:A rapid bead assay for detecting pathogenic bacteria with a simple microfluidic chip-based system was developed. Five oligonucleotide probes corresponding to the 16S rRNA of the targeted bacteria were coupled covalently to fluorescent beads. Four species of bacteria (Escherichia coli, Salmonella enterica subsp. enteri...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2006.03.014

    authors： Ikeda M,Yamaguchi N,Tani K,Nasu M

    更新日期：2006-11-01 00:00:00

  • Development and standardization of an in-house indirect ELISA for detection of duck antibody to fowl cholera.

    abstract：:Serological tests, such as agglutination and indirect hemagglutination assay (IHA), have been used to identify antibodies against Pasteurella multocida in poultry sera, but none are highly sensitive. An enzyme-linked immunosorbent assays (ELISA) has been used with varying degrees of success in attempts to monitor sero...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2017.08.018

    authors： Poolperm P,Varinrak T,Kataoka Y,Tragoolpua K,Sawada T,Sthitmatee N

    更新日期：2017-11-01 00:00:00

  • Development of culture medium using extruded bean as a nitrogen source for yeast growth.

    abstract：:In this study extruded bean was used as a nitrogen source substitute in culture medium formulation. A 3-factor simplex-lattice mixture design was used to establish better growth conditions. Completely substituted medium resulted in 43% of increase in the growth of Saccharomyces cerevisiae. Mixtures containing 1% extru...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2013.01.002

    authors： Batista KA,Bataus LA,Campos IT,Fernandes KF

    更新日期：2013-03-01 00:00:00

  • Whole-genome mapping for high-resolution genotyping of Pseudomonas aeruginosa.

    abstract：:A variety of molecular typing techniques have been developed to investigate the clonal relationship among bacterial isolates, including those associated with nosocomial infections. In this study, the authors evaluated whole-genome mapping as a tool to investigate the genetic relatedness between Pseudomonas aeruginosa ...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2014.07.020

    authors： Boers SA,Burggrave R,van Westreenen M,Goessens WHF,Hays JP

    更新日期：2014-11-01 00:00:00

  • Tryptic soy medium is feasible for the in situ preparation of standards containing small defined numbers of microbial cells.

    abstract：:A standard material comprising a few viable cells of a microorganism is essential for rational validation of microbiological methods. We propose a method of a flow cytometric sorting of cells stained with 6-carboxyfluorescein diacetate. The feasibility of tryptic soy medium in this method is demonstrated with 5 strain...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2013.01.021

    authors： Matsuoka H,Shigetomi T,Funabashi H,Saito M,Igimi S

    更新日期：2013-04-01 00:00:00

  • A comparative study of biofilm formation by Shiga toxigenic Escherichia coli using epifluorescence microscopy on stainless steel and a microtitre plate method.

    abstract：:Attachment of Shiga toxigenic Escherichia coli (STEC) to surfaces and the formation of biofilms may enhance persistence in a food processing environment and present a risk of contaminating products. Seven strains of STEC and three non-STEC strains were selected to compare two biofilm quantification methods; epifluores...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2006.11.014

    authors： Rivas L,Dykes GA,Fegan N

    更新日期：2007-04-01 00:00:00

  • Diabetic glucose meter for the determination of glucose in microbial cultures.

    abstract：:In wastewater, biological phosphate removal can fail because of the presence of glycogen accumulating organism (GAO), therefore measuring glycogen stored in microbial cultures provides information on the bacterial population type. Once glycogen is hydrolysed to glucose it was accurately measured using a human glucose ...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2014.03.002

    authors： Flavigny R

    更新日期：2014-05-01 00:00:00

  • The use of a rapid assay to detect the neuraminidase production in oral Porphyromonas spp. isolated from dogs and humans.

    abstract：:Neuraminidase was produced by 32.1% and 28.5% of Porphyromonas from dogs with and without periodontitis, respectively; and by 31.8% of bacteria from humans. The presence of neuraminidase in Porphyromonas spp. suggests that this enzyme can be involved with the pathogenesis of the periodontal disease, and the use of thi...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2013.06.017

    authors： de Assis PR,Nakano V,Senhorinho GN,Avila-Campos MJ

    更新日期：2013-09-01 00:00:00

  • TRICORE, a novel bead-based specimen concentration method for the culturing of Mycobacterium tuberculosis.

    abstract：:Centrifugation is a necessary concentrating step for the detection of Mycobacterium tuberculosis in a liquid culture. However, centrifugation is biologically hazardous and presents an obstacle in the development of an automated culture system. A bead-based bacterial concentration method, TRICORE, was recently develope...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2012.04.018

    authors： Mitarai S,Karinaga R,Yamada H,Mizuno K,Chikamatsu K,Aono A,Sugamoto T,Hatano T

    更新日期：2012-09-01 00:00:00

  • A PCR-based method for detection of potato pathogen, Helminthosporium solani, in silver scurf infected tuber tissue and soils.

    abstract：:Silver scurf caused by Helminthosporium solani causes significant economic losses in table stock, seed and processing potatoes. Specific polymerase chain reaction (PCR) primers, Hs1F1/Hs2R1, from H. solani were used for the amplification of a 447-bp product from 20 tissue samples and 54 single spore H. solani isolates...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/s0167-7012(00)00240-2

    authors： Errampalli D,Saunders J,Cullen D

    更新日期：2001-02-01 00:00:00

  • Enhancing recombinant protein solubility with ubiquitin-like small archeal modifying protein fusion partners.

    abstract：:A variety of protein expression tags with different biochemical properties has been used to enhance the yield and solubility of recombinant proteins. Ubiquitin, SUMO (small ubiquitin-like modifier) and prokaryotic ubiquitin like MoaD (molybdopterin synthase, small subunit) fusion tags are getting more popular because ...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2015.08.017

    authors： Varga S,Pathare GR,Baka E,Boicu M,Kriszt B,Székács A,Zinzula L,Kukolya J,Nagy I

    更新日期：2015-11-01 00:00:00

  • A simple method for high molecular-weight genomic DNA extraction suitable for long-read sequencing from spores of an obligate biotroph oomycete.

    abstract：:Long-read sequencing technologies are having a major impact on our approaches to studying non-model organisms and microbial communities. By significantly reducing the cost and facilitating the genome assembly pipelines, any laboratory can now develop its own genomics program regardless of the complexity of the genome ...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2020.106054

    authors： Penouilh-Suzette C,Fourré S,Besnard G,Godiard L,Pecrix Y

    更新日期：2020-09-12 00:00:00

  • A novel screening method based on menadione mediated rapid reduction of tetrazolium salt for testing of anti-mycobacterial agents.

    abstract：:A microplate-based rapid, inexpensive and robust technique is developed by using tetrazolium salt 2,3-bis[2-methyloxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-carboxanilide (XTT) and menadione to determine the viability of Mycobacterium tuberculosis, Mycobacterium bovis BCG and Mycobacterium smegmatis bacilli in microp...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2010.11.013

    authors： Singh U,Akhtar S,Mishra A,Sarkar D

    更新日期：2011-02-01 00:00:00